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P24923

- CDC21_MEDSA

UniProt

P24923 - CDC21_MEDSA

Protein

Cell division control protein 2 homolog 1

Gene

CDC2A

Organism
Medicago sativa (Alfalfa)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    Plays a key role in the control of the eukaryotic cell cycle. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Enzyme regulationi

    Phosphorylation at Thr-11 or Tyr-12 inactivates the enzyme, while phosphorylation at Thr-158 activates it.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei30 – 301ATPPROSITE-ProRule annotation
    Active sitei124 – 1241Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 159ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
    3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell division control protein 2 homolog 1 (EC:2.7.11.22, EC:2.7.11.23)
    Gene namesi
    Name:CDC2A
    OrganismiMedicago sativa (Alfalfa)
    Taxonomic identifieri3879 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 291›291Cell division control protein 2 homolog 1PRO_0000085753Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei11 – 111PhosphothreonineBy similarity
    Modified residuei12 – 121PhosphotyrosineBy similarity
    Modified residuei158 – 1581Phosphothreonine; by CAKBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP24923.

    Expressioni

    Tissue specificityi

    Found in most organs including root, young leaf, stem, vegetative meristem and flower bud.

    Structurei

    3D structure databases

    ProteinModelPortaliP24923.
    SMRiP24923. Positions 2-287.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 284284Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    P24923-1 [UniParc]FASTAAdd to Basket

    « Hide

    GENVEKIGEG TYGVVYKARD RVTNETIALK KIRLEQEDEG VPSTAIREIS    50
    LLKEMQHRNI VRLQDVVHSD KRLYLVFEYL DLDLKKHMDS SPEFIKDPRQ 100
    VKMFLYQMLC GIAYCHSHRV LHRDLKPQNL LIDRRTNSLK LADFGLARAF 150
    GIPVRTFTHE VVTLWYRAPE ILLGSRHYST PVDVWSVGCI FAEMANRRPL 200
    SPGDSEIDEL FKIFRILGTP NEDTWPGVTS LPDFKSTFPR WPSKDLATVV 250
    PNLEPAGLDL LNSMLCLDPT KRITARSAVE HEYFKDIKFV P 291
    Length:291
    Mass (Da):33,481
    Last modified:March 1, 1992 - v1
    Checksum:iE5D4F43E592D22B5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58365 mRNA. Translation: AAB41817.1.
    PIRiA39107.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58365 mRNA. Translation: AAB41817.1 .
    PIRi A39107.

    3D structure databases

    ProteinModelPortali P24923.
    SMRi P24923. Positions 2-287.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P24923.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complementation of a yeast cell cycle mutant by an alfalfa cDNA encoding a protein kinase homologous to p34cdc2."
      Hirt H., Pay A., Gyoergyey J., Bako L., Nemeth K., Boegre L., Schweyen R.J., Heberle-Bors E., Dudits D.
      Proc. Natl. Acad. Sci. U.S.A. 88:1636-1640(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiCDC21_MEDSA
    AccessioniPrimary (citable) accession number: P24923
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3