Reviewed, UniProtKB/Swiss-Prot P24923 (CDC21_MEDSA)

Names and origin

Protein names

Recommended name:
    Cell division control protein 2 homolog 1
    EC=2.7.11.22
    EC=2.7.11.23

Gene names

Name:

CDC2A

Organism

Medicago sativa (Alfalfa)

Taxonomic identifier

3879 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	291 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a key role in the control of the eukaryotic cell cycle. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Enzyme regulation	Phosphorylation at Thr-11 or Tyr-12 inactivates the enzyme, while phosphorylation at Thr-158 activates it By similarity.
Tissue specificity	Found in most organs including root, young leaf, stem, vegetative meristem and flower bud.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase subunit kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 291	›291	Cell division control protein 2 homolog 1		PRO_0000085753
Regions
	Domain	1 – 284	284	Protein kinase
	Nucleotide binding	7 – 15	9	ATP By similarity
Sites
	Active site	124	1	Proton acceptor By similarity
	Binding site	30	1	ATP By similarity
Amino acid modifications
	Modified residue	11	1	Phosphothreonine By similarity
	Modified residue	12	1	Phosphotyrosine By similarity
	Modified residue	158	1	Phosphothreonine; by CAK By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P24923-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: E5D4F43E592D22B5	FASTA	291	33,481
	10 20 30 40 50 60 GENVEKIGEG TYGVVYKARD RVTNETIALK KIRLEQEDEG VPSTAIREIS LLKEMQHRNI 70 80 90 100 110 120 VRLQDVVHSD KRLYLVFEYL DLDLKKHMDS SPEFIKDPRQ VKMFLYQMLC GIAYCHSHRV 130 140 150 160 170 180 LHRDLKPQNL LIDRRTNSLK LADFGLARAF GIPVRTFTHE VVTLWYRAPE ILLGSRHYST 190 200 210 220 230 240 PVDVWSVGCI FAEMANRRPL SPGDSEIDEL FKIFRILGTP NEDTWPGVTS LPDFKSTFPR 250 260 270 280 290 WPSKDLATVV PNLEPAGLDL LNSMLCLDPT KRITARSAVE HEYFKDIKFV P

References

[1]

"Complementation of a yeast cell cycle mutant by an alfalfa cDNA encoding a protein kinase homologous to p34cdc2." Hirt H., Pay A., Gyoergyey J., Bako L., Nemeth K., Boegre L., Schweyen R.J., Heberle-Bors E., Dudits D. Proc. Natl. Acad. Sci. U.S.A. 88:1636-1640(1991) [PubMed: 2000373] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M58365 mRNA. Translation: AAB41817.1.
PIR	A39107.
3D structure databases
HSSP	HSSP built from PDB template 1H06 based on UniProtKB P24941.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.22. 815. 2.7.11.23. 815.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDC21_MEDSA

Accession

Primary (citable) accession number: P24923

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)