P24920 (TRPC_PHYPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan biosynthesis protein TRP1

Including the following 2 domains:

1. Indole-3-glycerol phosphate synthase
   Short name=IGPS
   EC=4.1.1.48
2. N-(5'-phospho-ribosyl)anthranilate isomerase
   Short name=PRAI
   EC=5.3.1.24

Gene names

Name:

TRP1

Organism

Phytophthora parasitica (Potato buckeye rot agent)

Taxonomic identifier

4792 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Stramenopiles › Oomycetes › Peronosporales › Phytophthora

Protein attributes

Sequence length	531 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway.
Catalytic activity	N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate. 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5. Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.
Sequence similarities	In the N-terminal section; belongs to the TrpC family. In the C-terminal section; belongs to the TrpF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Decarboxylase Isomerase Lyase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	indole-3-glycerol-phosphate synthase activity Inferred from electronic annotation. Source: EC phosphoribosylanthranilate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 531	531	Tryptophan biosynthesis protein TRP1		PRO_0000154306
Regions
	Region	1 – 254	254	Indole-3-glycerol phosphate synthase
	Region	255 – 531	277	N-(5'-phosphoribosyl)anthranilate isomerase

Sequences

	Sequence		Length	Mass (Da)
	P24920 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: C154137A6A419ED1	FASTA	531	57,375
	10 20 30 40 50 60 MGNILEEIAA QRRLDVAAAK QVVSTDDLAK KIEHTESVYG PALPVLERLN APAEQVQAYA 70 80 90 100 110 120 NAGASMISVL TEPKWFKGSL DDMMEAREVV EGMSQRPAIL RKDFIIDVYQ LLEARAYGAD 130 140 150 160 170 180 CVLLIVTLLS KEQLIELIDA THNLGMCALV EVNSVQELDI ALAAKARLIG VNNRDLRTFK 190 200 210 220 230 240 VDMNTTARVA DAIRERGLSL GRDGVALFAL SGIRSHTDVV KYEKCGARGI LVGEYLMKSG 250 260 270 280 290 300 DIATTVKDLL QNVTRHSESG EFALLPPLAK VCGITTVEYA LAALRNGANM IGIIMAEHSP 310 320 330 340 350 360 RYVEKEEAKA IAKAVREYGE RTGPILSDIL ESHLDDKSDW FHRNVLALRE ACSRAPLVVG 370 380 390 400 410 420 VFVNKTATEM NAAAEEIGLD LVQLHGDEGF EICKDIKYPT IRALHLPDTA QCDGVDAEAV 430 440 450 460 470 480 LQQVSEGLAN YILLDTTVKG QQGGTGVAFD WKIAAIFTQA RLPCLMAGGL TPENVVKALS 490 500 510 520 530 VGHPVGVDVS SGVEVKGSPG VKDLDKVAAF LKAVKDHLSV ATLKIDEETE N

References

[1]

"The TRP1 gene of Phytophthora parasitica encoding indole-3-glycerolphosphate synthase-N-(5'-phosphoribosyl)anthranilate isomerase: structure and evolutionary distance from homologous fungal genes." Karlovsky P., Prell H.H. Gene 109:161-165(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64473 Genomic DNA. Translation: AAA33751.1.
3D structure databases
ProteinModelPortal	P24920.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	5.3.1.24. 4813.
UniPathway	UPA00035; UER00042. UPA00035; UER00043.
Family and domain databases
Gene3D	3.20.20.70. 3 hits.
InterPro	IPR013785. Aldolase_TIM. IPR013798. Indole-3-glycerol_P_synth. IPR001240. PRAI. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00218. IGPS. 1 hit. PF00697. PRAI. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 2 hits.
PROSITE	PS00614. IGPS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPC_PHYPR

Accession

Primary (citable) accession number: P24920

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)