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Protein

NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial

Gene

nuo78

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi68Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi79Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi82Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi96Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi128Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi132Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi135Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi141Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi182Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi185Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi188Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi232Iron-sulfur 3 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Protein family/group databases

TCDBi3.D.1.6.2 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-78kD
Short name:
CI-78kD
Gene namesi
Name:nuo78
ORF Names:B17C10.90, NCU01765
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 6, Linkage Group II

Organism-specific databases

EuPathDBiFungiDB:NCU01765

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33Mitochondrion1 PublicationAdd BLAST33
ChainiPRO_000001997434 – 744NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrialAdd BLAST711

Interactioni

Subunit structurei

Complex I is composed of about 40 different subunits.

Structurei

3D structure databases

ProteinModelPortaliP24918
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 1122Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST79
Domaini112 – 1514Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40
Domaini251 – 3074Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000031442
InParanoidiP24918
KOiK03934
OrthoDBiEOG092C0T2O

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
IPR015405 NuoG_C
PfamiView protein in Pfam
PF00384 Molybdopterin, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
PF09326 NADH_dhqG_C, 1 hit
SMARTiView protein in SMART
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24918-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRSTLSRSA WRTGRHQAAR NASRAFSATA QRPAEVELTI DGKKVSIEAG
60 70 80 90 100
SALIQACEKA GVTIPRYCYH EKLMIAGNCR MCLVEVEKVP KPVASCAWPV
110 120 130 140 150
QPGMVVKTNS PLTHKAREGV MEFLLANHPL DCPICDQGGE CDLQDQSMRY
160 170 180 190 200
GGDRGRFHEV GGKRAVEDKN MGPLIKTSMN RCIQCTRCVR FANDIAGAPE
210 220 230 240 250
LGSTGRGNDL QIGTYLEKNL DSELSGNVID LCPVGALTSK PYAFRARPWE
260 270 280 290 300
LKKTESIDVL DGLGSNIRVD TRGLEVMRIL PRLNDEVNEE WINDKTRFAC
310 320 330 340 350
DGLKTQRLTI PLVRREGKFE PASWDQALTE IAHAYQTLNP QGNEFKAIAG
360 370 380 390 400
QLTEVESLVA MKDLANRLGS ENLALDMPSG HKPLAHGVDV RSNYIFNSSI
410 420 430 440 450
VGIESADVIL LVGTNPRHEA AVLNARIRKQ WLRSDLEIGV VGQTWDSTFE
460 470 480 490 500
FEHLGTDHAA LQKALEGDFG KKLQSAKNPM IIVGSGVTDH GDANAFYETV
510 520 530 540 550
GKFVDSNASN FLTEEWNGYN VLQRAASRVG AFEVGFTVPS AEIAQTKPKF
560 570 580 590 600
VWLLGADEFN EADIPKDAFI VYQGHHGDRG AQIADIVLPG AAYTEKAGTY
610 620 630 640 650
VNTEGRVQMT RAATGLPGAA RTDWKILRAV SEYLGVRLPY DDVAQLRDRM
660 670 680 690 700
VEISPALSSY DIIEPPSLQQ LSKVQLVEQN QGATATNEPL KKVIENFYFT
710 720 730 740
DAISRSSPTM ARCSAAKKTG DPRTNFMAPG MEEDRPMGQI AYGA
Length:744
Mass (Da):81,602
Last modified:June 1, 2001 - v2
Checksum:iD842DDCE80510929
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti125L → P in CAA40828 (PubMed:1832016).Curated1
Sequence conflicti152G → R in CAA40828 (PubMed:1832016).Curated1
Sequence conflicti164R → Q in CAA40828 (PubMed:1832016).Curated1
Sequence conflicti340P → A in CAA40828 (PubMed:1832016).Curated1
Sequence conflicti379 – 388SGHKPLAHGV → FGPQTSCSWC in CAA40828 (PubMed:1832016).Curated10
Sequence conflicti493A → R in CAA40828 (PubMed:1832016).Curated1
Sequence conflicti527 – 534SRVGAFEV → PESAPSRL in CAA40828 (PubMed:1832016).Curated8
Sequence conflicti666 – 667PS → SL in CAA40828 (PubMed:1832016).Curated2
Sequence conflicti722P → S in CAA40828 (PubMed:1832016).Curated1
Sequence conflicti727 – 729MAP → IGS in CAA40828 (PubMed:1832016).Curated3
Sequence conflicti740I → Y in CAA40828 (PubMed:1832016).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57602 mRNA Translation: CAA40828.1
AL355926 Genomic DNA Translation: CAB91229.1
CM002237 Genomic DNA Translation: EAA27952.3
PIRiS17664
T49428
RefSeqiXP_957188.3, XM_952095.3

Genome annotation databases

EnsemblFungiiEAA27952; EAA27952; NCU01765
GeneIDi3873340
KEGGincr:NCU01765

Similar proteinsi

Entry informationi

Entry nameiNDUS1_NEUCR
AccessioniPrimary (citable) accession number: P24918
Secondary accession number(s): Q7RV66, Q9P6E0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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