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Protein

NADH-ubiquinone oxidoreductase 51 kDa subunit, mitochondrial

Gene

nuo-51

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • FMNCuratedNote: Binds 1 FMN.Curated
  • [4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi391Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi394Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi397Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi437Iron-sulfur (4Fe-4S)Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi96 – 105NADBy similarity10
Nucleotide bindingi212 – 259FMNBy similarityAdd BLAST48

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Protein family/group databases

TCDBi3.D.1.6.2 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 51 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-51kD
Short name:
CI-51kD
Gene namesi
Name:nuo-51
ORF Names:B10H18.210, NCU04044
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 5, Linkage Group VI

Organism-specific databases

EuPathDBiFungiDB:NCU04044

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionAdd BLAST27
ChainiPRO_000001998028 – 493NADH-ubiquinone oxidoreductase 51 kDa subunit, mitochondrialAdd BLAST466

Proteomic databases

PRIDEiP24917

Interactioni

Subunit structurei

Complex I is composed of about 40 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.

Structurei

3D structure databases

ProteinModelPortaliP24917
SMRiP24917
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 51 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000251534
InParanoidiP24917
KOiK03942
OMAiSGMKWSF
OrthoDBiEOG092C2XKH

Family and domain databases

Gene3Di1.20.1440.230, 1 hit
3.40.50.11540, 1 hit
InterProiView protein in InterPro
IPR001949 NADH-UbQ_OxRdtase_51kDa_CS
IPR011537 NADH-UbQ_OxRdtase_suF
IPR011538 Nuo51_FMN-bd
IPR037225 Nuo51_FMN-bd_sf
IPR019575 Nuop51_4Fe4S-bd
IPR037207 Nuop51_4Fe4S-bd_sf
IPR019554 Soluble_ligand-bd
PfamiView protein in Pfam
PF01512 Complex1_51K, 1 hit
PF10589 NADH_4Fe-4S, 1 hit
PF10531 SLBB, 1 hit
SMARTiView protein in SMART
SM00928 NADH_4Fe-4S, 1 hit
SUPFAMiSSF140490 SSF140490, 1 hit
SSF142019 SSF142019, 1 hit
TIGRFAMsiTIGR01959 nuoF_fam, 1 hit
PROSITEiView protein in PROSITE
PS00644 COMPLEX1_51K_1, 1 hit
PS00645 COMPLEX1_51K_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24917-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSRTAAPTK ASARTLSRAA AEQCRTFATV QDGSANPVRH YGGLKDQDRI
60 70 80 90 100
FQNLYGRYPP DLKHAKKMGD WHKTKEILLK GHDWIIGEVK ASGLRGRGGA
110 120 130 140 150
GFPSGLKWSF MNFKDWDKDD KPRYLVVNAD EGEPGTCKDR EIMRKDPHKL
160 170 180 190 200
VEGCLVAGRA MNATAAYIYI RGEFIQEAAI LQNAINEAYA DGLIGKNACG
210 220 230 240 250
SGYDFDVYLH RGAGAYVCGE ETSLIESLEG KPGKPRLKPP FPAAVGLFGC
260 270 280 290 300
PSTVANVETV AVAPTICRRG GNWFAGFGRE RNQGTKLFCI SGHVNNPCTV
310 320 330 340 350
EEEMSIPMRE LIDKHCGGVR GGWDNLLAVI PGGSSTPILP KHICDTQLMD
360 370 380 390 400
FDALKDSQSG LGTAALIVMD KSTDVVRAIS RLSHFYRHES CGQCTPCREG
410 420 430 440 450
SKWTEQIMKR FEKGQGRERE IDMLQELTKQ VEGHTICALG EAFAWPIQGL
460 470 480 490
IRHFRPELEA RIRKFAQENG GEALAGGWQR NARQQGKLVS PGM
Length:493
Mass (Da):54,241
Last modified:June 7, 2005 - v2
Checksum:i351B01F00602B06E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti260 – 261VA → AV in CAA39676 (PubMed:1832016).Curated2
Sequence conflicti442A → R in CAA39676 (PubMed:1832016).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56227 mRNA Translation: CAA39676.1
BX897678 Genomic DNA Translation: CAE85605.1
CM002241 Genomic DNA Translation: EAA28423.1
PIRiS17663
RefSeqiXP_957659.1, XM_952566.2

Genome annotation databases

EnsemblFungiiEAA28423; EAA28423; NCU04044
GeneIDi3873830
KEGGincr:NCU04044

Similar proteinsi

Entry informationi

Entry nameiNDUV1_NEUCR
AccessioniPrimary (citable) accession number: P24917
Secondary accession number(s): Q7RV82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: June 7, 2005
Last modified: March 28, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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