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Protein

Cytochrome P450 2F1

Gene

CYP2F1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the metabolism of various pneumotoxicants including naphthalene. Is able to dealkylate ethoxycoumarin, propoxycoumarin, and pentoxyresorufin but possesses no activity toward ethoxyresorufin and only trace dearylation activity toward benzyloxyresorufin. Bioactivates 3-methylindole (3MI) by dehydrogenation to the putative electrophile 3-methylene-indolenine.1 Publication

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.3 Publications

Cofactori

hemeBy similarity

Kineticsi

  1. KM=0.018 mM for 3-methylindole2 Publications
  1. Vmax=51.2 pmol/min/mg enzyme toward ethoxy-coumarin2 Publications
  2. Vmax=24.3 pmol/min/mg enzyme toward propoxy-coumarin2 Publications
  3. Vmax=22 pmol/min/mg enzyme toward pentoxy-resorufin2 Publications
  4. Vmax=2.29 pmol/min/mg enzyme toward benzyloxy-resorufin2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi436Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:HS05409-MONOMER.
ReactomeiR-HSA-211935. Fatty acids.
R-HSA-211981. Xenobiotics.
R-HSA-211999. CYP2E1 reactions.
SABIO-RKP24903.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2F1 (EC:1.14.14.13 Publications)
Alternative name(s):
CYPIIF1
Gene namesi
Name:CYP2F1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:2632. CYP2F1.

Subcellular locationi

  • Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Microsome membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

DisGeNETi1572.
OpenTargetsiENSG00000197446.
PharmGKBiPA27109.

Polymorphism and mutation databases

BioMutaiCYP2F1.
DMDMi259016202.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000517591 – 491Cytochrome P450 2F1Add BLAST491

Proteomic databases

PaxDbiP24903.
PeptideAtlasiP24903.
PRIDEiP24903.

PTM databases

iPTMnetiP24903.
PhosphoSitePlusiP24903.

Expressioni

Tissue specificityi

Expressed in lung. Rarely detected in liver and placenta.2 Publications

Gene expression databases

BgeeiENSG00000197446.
CleanExiHS_CYP2F1.
ExpressionAtlasiP24903. baseline and differential.
GenevisibleiP24903. HS.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000333534.

Structurei

3D structure databases

ProteinModelPortaliP24903.
SMRiP24903.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118775.
HOVERGENiHBG015789.
InParanoidiP24903.
KOiK07416.
OMAiWGEASEI.
OrthoDBiEOG091G0BT8.
PhylomeDBiP24903.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR020469. Cyt_P450_CYP2_fam.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01957. EP450ICYP2F.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P24903-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSISTAILL LLLALVCLLL TLSSRDKGKL PPGPRPLSIL GNLLLLCSQD
60 70 80 90 100
MLTSLTKLSK EYGSMYTVHL GPRRVVVLSG YQAVKEALVD QGEEFSGRGD
110 120 130 140 150
YPAFFNFTKG NGIAFSSGDR WKVLRQFSIQ ILRNFGMGKR SIEERILEEG
160 170 180 190 200
SFLLAELRKT EGEPFDPTFV LSRSVSNIIC SVLFGSRFDY DDERLLTIIR
210 220 230 240 250
LINDNFQIMS SPWGELYDIF PSLLDWVPGP HQRIFQNFKC LRDLIAHSVH
260 270 280 290 300
DHQASLDPRS PRDFIQCFLT KMAEEKEDPL SHFHMDTLLM TTHNLLFGGT
310 320 330 340 350
KTVSTTLHHA FLALMKYPKV QARVQEEIDL VVGRARLPAL KDRAAMPYTD
360 370 380 390 400
AVIHEVQRFA DIIPMNLPHR VTRDTAFRGF LIPKGTDVIT LLNTVHYDPS
410 420 430 440 450
QFLTPQEFNP EHFLDANQSF KKSPAFMPFS AGRRLCLGES LARMELFLYL
460 470 480 490
TAILQSFSLQ PLGAPEDIDL TPLSSGLGNL PRPFQLCLRP R
Length:491
Mass (Da):55,501
Last modified:September 22, 2009 - v2
Checksum:iD8022271FC0B6D06
GO
Isoform 2 (identifier: P24903-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     303-320: VSTTLHHAFLALMKYPKV → PACRRRSTSWWDARGCRR
     321-491: Missing.

Note: No experimental confirmation available.
Show »
Length:320
Mass (Da):36,410
Checksum:i19FA98DD0E534DBB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156 – 157EL → DV in AAA52156 (PubMed:1974816).Curated2
Sequence conflicti219I → ILDPR in AAA52156 (PubMed:1974816).Curated1
Sequence conflicti219I → ILDPR in AAL69652 (PubMed:11827709).Curated1
Sequence conflicti256 – 259Missing in AAA52156 (PubMed:1974816).Curated4
Sequence conflicti440S → L in AAA52156 (PubMed:1974816).Curated1

Polymorphismi

Eight non disease-associated alleles are known: CYP2F1*1, CYP2F1*2A, CYP2F1*2B, CYP2F1*3, CYP2F1*4, CYP2F1*5A, CYP2F1*5B and CYP2F1*6. The sequence shown corresponds to allele CYP2F1*1.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05886338S → P in allele CYP2F1*4. Corresponds to variant rs58285195dbSNPEnsembl.1
Natural variantiVAR_05886498R → P in allele CYP2F1*6. Corresponds to variant rs57670668dbSNPEnsembl.1
Natural variantiVAR_058865218D → N in allele CYP2F1*3 and in allele CYP2F1*4. Corresponds to variant rs80319612dbSNPEnsembl.1
Natural variantiVAR_058866266Q → H in allele CYP2F1*3. 1 PublicationCorresponds to variant rs75405062dbSNPEnsembl.1
Natural variantiVAR_058867391L → P in allele CYP2F1*5A and in allele CYP2F1*5B. Corresponds to variant rs144315434dbSNPEnsembl.1
Natural variantiVAR_058868490P → L in allele CYP2F1*3. Corresponds to variant rs7246981dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055575303 – 320VSTTL…KYPKV → PACRRRSTSWWDARGCRR in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_055576321 – 491Missing in isoform 2. 1 PublicationAdd BLAST171

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02906 mRNA. Translation: AAA52156.1.
AF372573
, AF372570, AF372571, AF372572 Genomic DNA. Translation: AAL69652.1.
EF122241 Genomic DNA. Translation: ABO41976.1.
EF122242 Genomic DNA. Translation: ABO41977.1.
EF122243 Genomic DNA. Translation: ABO41978.1.
EF122244 Genomic DNA. Translation: ABO41979.1.
EF122245 Genomic DNA. Translation: ABO41980.1.
AC008962 Genomic DNA. No translation available.
BC109056 mRNA. Translation: AAI09057.1.
CCDSiCCDS12572.1. [P24903-1]
PIRiA36036.
B36036.
RefSeqiNP_000765.2. NM_000774.4. [P24903-1]
UniGeneiHs.558318.
Hs.676472.

Genome annotation databases

EnsembliENST00000331105; ENSP00000333534; ENSG00000197446. [P24903-1]
ENST00000532164; ENSP00000471416; ENSG00000197446. [P24903-2]
GeneIDi1572.
KEGGihsa:1572.
UCSCiuc002opu.2. human. [P24903-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Cytochrome P450 Allele Nomenclature Committee

CYP2F1 alleles

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02906 mRNA. Translation: AAA52156.1.
AF372573
, AF372570, AF372571, AF372572 Genomic DNA. Translation: AAL69652.1.
EF122241 Genomic DNA. Translation: ABO41976.1.
EF122242 Genomic DNA. Translation: ABO41977.1.
EF122243 Genomic DNA. Translation: ABO41978.1.
EF122244 Genomic DNA. Translation: ABO41979.1.
EF122245 Genomic DNA. Translation: ABO41980.1.
AC008962 Genomic DNA. No translation available.
BC109056 mRNA. Translation: AAI09057.1.
CCDSiCCDS12572.1. [P24903-1]
PIRiA36036.
B36036.
RefSeqiNP_000765.2. NM_000774.4. [P24903-1]
UniGeneiHs.558318.
Hs.676472.

3D structure databases

ProteinModelPortaliP24903.
SMRiP24903.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000333534.

PTM databases

iPTMnetiP24903.
PhosphoSitePlusiP24903.

Polymorphism and mutation databases

BioMutaiCYP2F1.
DMDMi259016202.

Proteomic databases

PaxDbiP24903.
PeptideAtlasiP24903.
PRIDEiP24903.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000331105; ENSP00000333534; ENSG00000197446. [P24903-1]
ENST00000532164; ENSP00000471416; ENSG00000197446. [P24903-2]
GeneIDi1572.
KEGGihsa:1572.
UCSCiuc002opu.2. human. [P24903-1]

Organism-specific databases

CTDi1572.
DisGeNETi1572.
GeneCardsiCYP2F1.
H-InvDBHIX0202929.
HGNCiHGNC:2632. CYP2F1.
MIMi124070. gene.
neXtProtiNX_P24903.
OpenTargetsiENSG00000197446.
PharmGKBiPA27109.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118775.
HOVERGENiHBG015789.
InParanoidiP24903.
KOiK07416.
OMAiWGEASEI.
OrthoDBiEOG091G0BT8.
PhylomeDBiP24903.
TreeFamiTF352043.

Enzyme and pathway databases

BioCyciZFISH:HS05409-MONOMER.
ReactomeiR-HSA-211935. Fatty acids.
R-HSA-211981. Xenobiotics.
R-HSA-211999. CYP2E1 reactions.
SABIO-RKP24903.

Miscellaneous databases

GeneWikiiCYP2F1.
GenomeRNAii1572.
PROiP24903.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000197446.
CleanExiHS_CYP2F1.
ExpressionAtlasiP24903. baseline and differential.
GenevisibleiP24903. HS.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR020469. Cyt_P450_CYP2_fam.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01957. EP450ICYP2F.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP2F1_HUMAN
AccessioniPrimary (citable) accession number: P24903
Secondary accession number(s): A7KAU6
, A7KAU7, A7KAU8, A7KAU9, A7KAV0, Q32MN5, Q8WWJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: September 22, 2009
Last modified: November 2, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.