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P24903 (CP2F1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2F1

EC=1.14.14.1
Alternative name(s):
CYPIIF1
Gene names
Name:CYP2F1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in the metabolism of various pneumotoxicants including naphthalene. Is able to dealkylate ethoxycoumarin, propoxycoumarin, and pentoxyresorufin but possesses no activity toward ethoxyresorufin and only trace dearylation activity toward benzyloxyresorufin. Bioactivates 3-methylindole (3MI) by dehydrogenation to the putative electrophile 3-methylene-indolenine. Ref.1

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. Ref.1 Ref.5 Ref.6

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein Probable. Microsome membrane; Peripheral membrane protein Ref.6.

Tissue specificity

Expressed in lung. Rarely detected in liver and placenta. Ref.1 Ref.4

Polymorphism

Eight non disease-associated alleles are known: CYP2F1*1, CYP2F1*2A, CYP2F1*2B, CYP2F1*3, CYP2F1*4, CYP2F1*5A, CYP2F1*5B and CYP2F1*6. The sequence shown corresponds to allele CYP2F1*1.

Sequence similarities

Belongs to the cytochrome P450 family.

Biophysicochemical properties

Kinetic parameters:

KM=0.018 mM for 3-methylindole Ref.1 Ref.6

Vmax=51.2 pmol/min/mg enzyme toward ethoxy-coumarin

Vmax=24.3 pmol/min/mg enzyme toward propoxy-coumarin

Vmax=22 pmol/min/mg enzyme toward pentoxy-resorufin

Vmax=2.29 pmol/min/mg enzyme toward benzyloxy-resorufin

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   Coding sequence diversityPolymorphism
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnaphthalene metabolic process

Inferred from direct assay Ref.6. Source: UniProtKB

response to toxic substance

Inferred from electronic annotation. Source: Ensembl

small molecule metabolic process

Traceable author statement. Source: Reactome

trichloroethylene metabolic process

Inferred from electronic annotation. Source: Ensembl

xenobiotic metabolic process

Traceable author statement. Source: Reactome

   Cellular_componentendoplasmic reticulum membrane

Traceable author statement. Source: Reactome

intracellular membrane-bounded organelle

Inferred from direct assay Ref.6. Source: UniProtKB

   Molecular_functionaromatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

monooxygenase activity

Traceable author statement Ref.1. Source: ProtInc

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen

Inferred from direct assay Ref.6. Source: UniProtKB

oxygen binding

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Cytochrome P450 2F1
PRO_0000051759

Sites

Metal binding4361Iron (heme axial ligand) By similarity

Natural variations

Natural variant381S → P in allele CYP2F1*4.
Corresponds to variant rs58285195 [ dbSNP | Ensembl ].
VAR_058863
Natural variant981R → P in allele CYP2F1*6.
Corresponds to variant rs57670668 [ dbSNP | Ensembl ].
VAR_058864
Natural variant2181D → N in allele CYP2F1*3 and in allele CYP2F1*4.
Corresponds to variant rs80319612 [ dbSNP | Ensembl ].
VAR_058865
Natural variant2661Q → H in allele CYP2F1*3. Ref.2
VAR_058866
Natural variant3911L → P in allele CYP2F1*5A and in allele CYP2F1*5B.
Corresponds to variant rs144315434 [ dbSNP | Ensembl ].
VAR_058867
Natural variant4901P → L in allele CYP2F1*3.
Corresponds to variant rs7246981 [ dbSNP | Ensembl ].
VAR_058868

Experimental info

Sequence conflict156 – 1572EL → DV in AAA52156. Ref.1
Sequence conflict2191I → ILDPR in AAA52156. Ref.1
Sequence conflict2191I → ILDPR in AAL69652. Ref.2
Sequence conflict256 – 2594Missing in AAA52156. Ref.1
Sequence conflict4401S → L in AAA52156. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24903 [UniParc].

Last modified September 22, 2009. Version 2.
Checksum: D8022271FC0B6D06

FASTA49155,501
        10         20         30         40         50         60 
MDSISTAILL LLLALVCLLL TLSSRDKGKL PPGPRPLSIL GNLLLLCSQD MLTSLTKLSK 

        70         80         90        100        110        120 
EYGSMYTVHL GPRRVVVLSG YQAVKEALVD QGEEFSGRGD YPAFFNFTKG NGIAFSSGDR 

       130        140        150        160        170        180 
WKVLRQFSIQ ILRNFGMGKR SIEERILEEG SFLLAELRKT EGEPFDPTFV LSRSVSNIIC 

       190        200        210        220        230        240 
SVLFGSRFDY DDERLLTIIR LINDNFQIMS SPWGELYDIF PSLLDWVPGP HQRIFQNFKC 

       250        260        270        280        290        300 
LRDLIAHSVH DHQASLDPRS PRDFIQCFLT KMAEEKEDPL SHFHMDTLLM TTHNLLFGGT 

       310        320        330        340        350        360 
KTVSTTLHHA FLALMKYPKV QARVQEEIDL VVGRARLPAL KDRAAMPYTD AVIHEVQRFA 

       370        380        390        400        410        420 
DIIPMNLPHR VTRDTAFRGF LIPKGTDVIT LLNTVHYDPS QFLTPQEFNP EHFLDANQSF 

       430        440        450        460        470        480 
KKSPAFMPFS AGRRLCLGES LARMELFLYL TAILQSFSLQ PLGAPEDIDL TPLSSGLGNL 

       490 
PRPFQLCLRP R 

« Hide

References

[1]"The human CYP2F gene subfamily: identification of a cDNA encoding a new cytochrome P450, cDNA-directed expression, and chromosome mapping."
Nhamburo P.T., Kimura S., McBride O.W., Kozak C.A., Gelboin H.V., Gonzalez F.J.
Biochemistry 29:5491-5499(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
Tissue: Lung.
[2]"Identification and cross-species comparisons of CYP2F subfamily genes in mammals."
Chen N., Whitehead S.E., Caillat A.W., Gavit K., Isphording D.R., Kovacevic D., McCreary M.B., Hoffman S.M.G.
Mutat. Res. 499:155-161(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT HIS-266.
[3]"Molecular analysis of the CYP2F1 gene: identification of a frequent non-functional allelic variant."
Tournel G., Cauffiez C., Billaut-Laden I., Allorge D., Chevalier D., Bonnifet F., Mensier E., Lafitte J.-J., Lhermitte M., Broly F., Lo-Guidice J.-M.
Mutat. Res. 617:79-89(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELES CYP2F1*1; CYP2F1*2A; CYP2F1*2B; CYP2F1*3; CYP2F1*4; CYP2F1*5A; CYP2F1*5B AND CYP2F1*6).
[4]"Expression of xenobiotic-metabolizing cytochrome P450 forms in human full-term placenta."
Hakkola J., Pasanen M., Hukkanen J., Pelkonen O., Maeenpaeae J., Edwards R.J., Boobis A.R., Raunio H.
Biochem. Pharmacol. 51:403-411(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Metabolism of 3-methylindole by vaccinia-expressed P450 enzymes: correlation of 3-methyleneindolenine formation and protein-binding."
Thornton-Manning J., Appleton M.L., Gonzalez F.J., Yost G.S.
J. Pharmacol. Exp. Ther. 276:21-29(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY.
[6]"Specific dehydrogenation of 3-methylindole and epoxidation of naphthalene by recombinant human CYP2F1 expressed in lymphoblastoid cells."
Lanza D.L., Code E., Crespi C.L., Gonzalez F.J., Yost G.S.
Drug Metab. Dispos. 27:798-803(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02906 mRNA. Translation: AAA52156.1.
AF372573 expand/collapse EMBL AC list , AF372570, AF372571, AF372572 Genomic DNA. Translation: AAL69652.1.
EF122241 Genomic DNA. Translation: ABO41976.1.
EF122242 Genomic DNA. Translation: ABO41977.1.
EF122243 Genomic DNA. Translation: ABO41978.1.
EF122244 Genomic DNA. Translation: ABO41979.1.
EF122245 Genomic DNA. Translation: ABO41980.1.
CCDSCCDS12572.1.
PIRA36036.
B36036.
RefSeqNP_000765.2. NM_000774.3.
UniGeneHs.558318.

3D structure databases

ProteinModelPortalP24903.
SMRP24903. Positions 27-491.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000333534.

PTM databases

PhosphoSiteP24903.

Polymorphism databases

DMDM259016202.

Proteomic databases

PaxDbP24903.
PRIDEP24903.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000331105; ENSP00000333534; ENSG00000197446.
GeneID1572.
KEGGhsa:1572.
UCSCuc002opu.1. human.

Organism-specific databases

CTD1572.
GeneCardsGC19P041620.
H-InvDBHIX0202929.
HGNCHGNC:2632. CYP2F1.
MIM124070. gene.
neXtProtNX_P24903.
PharmGKBPA27109.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2124.
HOVERGENHBG015789.
InParanoidP24903.
KOK07416.
OMALRHAFLV.
OrthoDBEOG7RBZ85.
PhylomeDBP24903.
TreeFamTF352043.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.

Gene expression databases

BgeeP24903.
CleanExHS_CYP2F1.
GenevestigatorP24903.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR020469. Cyt_P450_CYP2_fam.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01957. EP450ICYP2F.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCYP2F1.
GenomeRNAi1572.
NextBio6465.
PROP24903.
SOURCESearch...

Entry information

Entry nameCP2F1_HUMAN
AccessionPrimary (citable) accession number: P24903
Secondary accession number(s): A7KAU6 expand/collapse secondary AC list , A7KAU7, A7KAU8, A7KAU9, A7KAV0, Q8WWJ2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: September 22, 2009
Last modified: July 9, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM