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Protein

G2/mitotic-specific cyclin-4

Gene

CLB4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the CDC2 protein kinase to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.

GO - Molecular functioni

  • cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • G2/M transition of mitotic cell cycle Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • positive regulation of spindle pole body separation Source: SGD
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD
  • regulation of mitotic spindle assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32327-MONOMER.
ReactomeiR-SCE-113507. E2F-enabled inhibition of pre-replication complex formation.
R-SCE-113510. E2F mediated regulation of DNA replication.
R-SCE-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-SCE-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-SCE-3214858. RMTs methylate histone arginines.
R-SCE-4419969. Depolymerisation of the Nuclear Lamina.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-SCE-69202. Cyclin E associated events during G1/S transition.
R-SCE-69205. G1/S-Specific Transcription.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.
R-SCE-69478. G2/M DNA replication checkpoint.
R-SCE-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-SCE-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
G2/mitotic-specific cyclin-4
Gene namesi
Name:CLB4
Ordered Locus Names:YLR210W
ORF Names:L8167.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR210W.
SGDiS000004200. CLB4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460G2/mitotic-specific cyclin-4PRO_0000080406Add
BLAST

PTM databases

iPTMnetiP24871.

Interactioni

Protein-protein interaction databases

BioGridi31478. 63 interactions.
DIPiDIP-2268N.
IntActiP24871. 3 interactions.
MINTiMINT-486096.

Structurei

3D structure databases

ProteinModelPortaliP24871.
SMRiP24871. Positions 206-450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family. Cyclin AB subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00760000118939.
HOGENOMiHOG000167672.
InParanoidiP24871.
KOiK06659.
OMAiIQDENAN.
OrthoDBiEOG092C2S0G.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 2 hits.
SM01332. Cyclin_C. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLEGYTVQP PQSTLIGDIE IQDENANQEV KNVLYQGVQK GIKRLEKRQR
60 70 80 90 100
RVALGDVTSQ KANKIHNAIH NKFHQTKNNF EIENIRSSAL VKEQQRDVRH
110 120 130 140 150
EDSDYFLIDS SEGSSTDDEQ VNEDAIDDLL SRRVNDQQIQ ADEVYEDFDG
160 170 180 190 200
EMQDVIEEDV DSQIEPLSPI NNDEIQTELD RAFEKYFRSV PNPLDDDTHD
210 220 230 240 250
VVMVVEYASD IFYYLRELEV KYRPNPYYMQ NQVELTWPFR RTMIDWLVQL
260 270 280 290 300
HFRFQLLPET LYLTINIVDR FLSKKTVTLN RFQLVGVSAL FIAAKFEEIN
310 320 330 340 350
CPTLDDLVYM LENTYTRDDI IRAEQYMIDT LEFEIGWPGP MPFLRRISKA
360 370 380 390 400
DDYDFEPRTL AKYLLETTIV EPKLVAAAPS WLAAGAYFLS RTILGSNDWS
410 420 430 440 450
LKHVFYSGYT SSQIIPLASL ILENCKNASR RHHSIWKKYF DQKHYRCSQI
460
VEEWIVSTEA
Length:460
Mass (Da):53,853
Last modified:October 1, 1993 - v2
Checksum:i2BAFCEC706F2FBA2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti255 – 2551Q → R no nucleotide entry (PubMed:1849457).Curated
Sequence conflicti318 – 3181D → S no nucleotide entry (PubMed:1849457).Curated
Sequence conflicti328 – 3281I → T no nucleotide entry (PubMed:1849457).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69426 Genomic DNA. Translation: CAA49202.1.
M80303 Genomic DNA. Translation: AAA73136.1.
U14913 Genomic DNA. Translation: AAB67425.1.
BK006945 Genomic DNA. Translation: DAA09527.1.
PIRiB60048.
RefSeqiNP_013311.1. NM_001182097.1.

Genome annotation databases

EnsemblFungiiYLR210W; YLR210W; YLR210W.
GeneIDi850907.
KEGGisce:YLR210W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69426 Genomic DNA. Translation: CAA49202.1.
M80303 Genomic DNA. Translation: AAA73136.1.
U14913 Genomic DNA. Translation: AAB67425.1.
BK006945 Genomic DNA. Translation: DAA09527.1.
PIRiB60048.
RefSeqiNP_013311.1. NM_001182097.1.

3D structure databases

ProteinModelPortaliP24871.
SMRiP24871. Positions 206-450.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31478. 63 interactions.
DIPiDIP-2268N.
IntActiP24871. 3 interactions.
MINTiMINT-486096.

PTM databases

iPTMnetiP24871.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR210W; YLR210W; YLR210W.
GeneIDi850907.
KEGGisce:YLR210W.

Organism-specific databases

EuPathDBiFungiDB:YLR210W.
SGDiS000004200. CLB4.

Phylogenomic databases

GeneTreeiENSGT00760000118939.
HOGENOMiHOG000167672.
InParanoidiP24871.
KOiK06659.
OMAiIQDENAN.
OrthoDBiEOG092C2S0G.

Enzyme and pathway databases

BioCyciYEAST:G3O-32327-MONOMER.
ReactomeiR-SCE-113507. E2F-enabled inhibition of pre-replication complex formation.
R-SCE-113510. E2F mediated regulation of DNA replication.
R-SCE-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-SCE-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-SCE-3214858. RMTs methylate histone arginines.
R-SCE-4419969. Depolymerisation of the Nuclear Lamina.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-SCE-69202. Cyclin E associated events during G1/S transition.
R-SCE-69205. G1/S-Specific Transcription.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.
R-SCE-69478. G2/M DNA replication checkpoint.
R-SCE-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-SCE-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Miscellaneous databases

PROiP24871.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 2 hits.
SM01332. Cyclin_C. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCG24_YEAST
AccessioniPrimary (citable) accession number: P24871
Secondary accession number(s): D6VYL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 1, 1993
Last modified: September 7, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 98 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.