P24870 (CG23_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G2/mitotic-specific cyclin-3

Gene names

Name:	CLB3
Ordered Locus Names:	YDL155W
ORF Names:	D1539

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the CDC2 protein kinase to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.
Miscellaneous	Present with 892 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the cyclin family. Cyclin AB subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Molecular function	Cyclin
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G2/M transition of mitotic cell cycle Inferred from mutant phenotype Ref.1. Source: SGD S phase of mitotic cell cycle Inferred from genetic interaction Ref.1. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of G2/M transition of mitotic cell cycle Inferred from electronic annotation. Source: InterPro regulation of cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	protein kinase binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC14	Q00684	2	EBI-4522,EBI-4192
CKS1	P20486	5	EBI-4522,EBI-4746

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	G2/mitotic-specific cyclin-3		PRO_0000080405
Amino acid modifications
	Modified residue	33	1	Phosphoserine Ref.9
Experimental info
	Sequence conflict	48	1	D → R in CAA49201. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24870 [UniParc]. Last modified October 25, 2004. Version 3. Checksum: 91EE26655A5EC72D	FASTA	427	49,476
	10 20 30 40 50 60 MHHNSQSLSS GHIRSPEDEN VAPIGNLKHR TGSLSHISSA HPRVALSDVT NIVATNSSNN 70 80 90 100 110 120 SISKPKVAPI KERLDSAAII EEERLDANSV AQRKEADHND LLTDREQEEP VEDDGESEED 130 140 150 160 170 180 EEEDQEPLLL QHYASDTLVW EHAFRTYYRT TLDPNDDDVY DVVMVAELSN EIFEYMRKLE 190 200 210 220 230 240 DLYKPNPYYM DKQPELRWSF RSTLIDWIVQ VHEKFQLLPE TLYLCINIID RYLCKEVVPV 250 260 270 280 290 300 NKFQLVGAAS LFIAAKYEEI NCPTIKDFVY MSENCYSRND LLDAERTILN GLEFELGWPG 310 320 330 340 350 360 PMSFLRRISK ADDYEHDTRT LAKYLLESTI MDHRLVSAQP SWLAAGAYFL SKIILGQNQW 370 380 390 400 410 420 SLAHVYYSNY TQEQILPLAT IILENCRYAS KRHNAIWRKY SSRRYLHSSQ IVAKWIALAE HRVERSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cyclin-B homologs in Saccharomyces cerevisiae function in S phase and in G2." Richardson H., Lew D.J., Henze M., Sugimoto K., Reed S.I. Genes Dev. 6:2021-2034(1992) [PubMed: 1427070] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae." Fitch I., Dahmann C., Surana U., Amon A., Nasmyth K., Goetsch L., Byers B., Futcher B. Mol. Biol. Cell 3:805-818(1992) [PubMed: 1387566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Analysis of a 23 kb region on the left arm of yeast chromosome IV." Delaveau T.T.D., Blugeon C., Jacq C., Perea J. Yeast 12:1587-1592(1996) [PubMed: 8972581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae." Surana U., Robitsch H., Price C., Schuster T., Fitch I., Futcher A.B., Nasmyth K. Cell 65:145-161(1991) [PubMed: 1849457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 194-383.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69425 Genomic DNA. Translation: CAA49201.1. M80302 Genomic DNA. Translation: AAA34765.1. X97751 Genomic DNA. Translation: CAA66336.1. Z74203 Genomic DNA. Translation: CAA98729.1. AY692856 Genomic DNA. Translation: AAT92875.1. BK006938 Genomic DNA. Translation: DAA11705.1.
PIR	A60048.
RefSeq	NP_010126.1. NM_001180215.1.
3D structure databases
ProteinModelPortal	P24870.
SMR	P24870. Positions 165-413.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1266N.
IntAct	P24870. 31 interactions.
MINT	MINT-411020.
STRING	P24870.
Proteomic databases
PeptideAtlas	P24870.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDL155W; YDL155W; YDL155W.
GeneID	851400.
KEGG	sce:YDL155W.
NMPDR	fig|4932.3.peg.863.
Organism-specific databases
CYGD	YDL155w.
SGD	S000002314. CLB3.
Phylogenomic databases
eggNOG	fuNOG04497.
GeneTree	EFGT00050000003593.
HOGENOM	HBG750484.
OMA	YLLESTI.
OrthoDB	EOG4QNR55.
Gene expression databases
ArrayExpress	P24870.
Genevestigator	P24870.
GermOnline	YDL155W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013763. Cyclin-like. IPR014400. Cyclin_A/B/D/E. IPR015454. Cyclin_B. IPR004367. Cyclin_C. IPR006671. Cyclin_N. [Graphical view]
Gene3D	G3DSA:1.10.472.10. Cyclin_related. 2 hits.
KO	K06659.
PANTHER	PTHR10177:SF27. CycB. 1 hit.
Pfam	PF02984. Cyclin_C. 1 hit. PF00134. Cyclin_N. 1 hit. [Graphical view]
PIRSF	PIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMART	SM00385. CYCLIN. 2 hits. [Graphical view]
SUPFAM	SSF47954. Cyclin_like. 2 hits.
PROSITE	PS00292. CYCLINS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968567.

Entry information

Entry name

CG23_YEAST

Accession

Primary (citable) accession number: P24870
Secondary accession number(s): D6VRJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 106 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program