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Protein

G2/mitotic-specific cyclin-3

Gene

CLB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the CDC2 protein kinase to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.

GO - Molecular functioni

  • cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • G2/M transition of mitotic cell cycle Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • positive regulation of spindle pole body separation Source: SGD
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD
  • regulation of mitotic spindle assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-29550-MONOMER.
ReactomeiR-SCE-113507. E2F-enabled inhibition of pre-replication complex formation.
R-SCE-113510. E2F mediated regulation of DNA replication.
R-SCE-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-SCE-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-SCE-3214858. RMTs methylate histone arginines.
R-SCE-4419969. Depolymerisation of the Nuclear Lamina.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-SCE-69202. Cyclin E associated events during G1/S transition.
R-SCE-69205. G1/S-Specific Transcription.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.
R-SCE-69478. G2/M DNA replication checkpoint.
R-SCE-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-SCE-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
G2/mitotic-specific cyclin-3
Gene namesi
Name:CLB3
Ordered Locus Names:YDL155W
ORF Names:D1539
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL155W.
SGDiS000002314. CLB3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427G2/mitotic-specific cyclin-3PRO_0000080405Add
BLAST

PTM databases

iPTMnetiP24870.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC14Q006842EBI-4522,EBI-4192
CKS1P204864EBI-4522,EBI-4746

Protein-protein interaction databases

BioGridi31908. 107 interactions.
DIPiDIP-1266N.
IntActiP24870. 31 interactions.
MINTiMINT-411020.

Structurei

3D structure databases

ProteinModelPortaliP24870.
SMRiP24870. Positions 166-411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family. Cyclin AB subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00760000118939.
HOGENOMiHOG000167672.
InParanoidiP24870.
KOiK06659.
OMAiYLLESTI.
OrthoDBiEOG7FNCJX.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 2 hits.
SM01332. Cyclin_C. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHHNSQSLSS GHIRSPEDEN VAPIGNLKHR TGSLSHISSA HPRVALSDVT
60 70 80 90 100
NIVATNSSNN SISKPKVAPI KERLDSAAII EEERLDANSV AQRKEADHND
110 120 130 140 150
LLTDREQEEP VEDDGESEED EEEDQEPLLL QHYASDTLVW EHAFRTYYRT
160 170 180 190 200
TLDPNDDDVY DVVMVAELSN EIFEYMRKLE DLYKPNPYYM DKQPELRWSF
210 220 230 240 250
RSTLIDWIVQ VHEKFQLLPE TLYLCINIID RYLCKEVVPV NKFQLVGAAS
260 270 280 290 300
LFIAAKYEEI NCPTIKDFVY MSENCYSRND LLDAERTILN GLEFELGWPG
310 320 330 340 350
PMSFLRRISK ADDYEHDTRT LAKYLLESTI MDHRLVSAQP SWLAAGAYFL
360 370 380 390 400
SKIILGQNQW SLAHVYYSNY TQEQILPLAT IILENCRYAS KRHNAIWRKY
410 420
SSRRYLHSSQ IVAKWIALAE HRVERSN
Length:427
Mass (Da):49,476
Last modified:October 25, 2004 - v3
Checksum:i91EE26655A5EC72D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481D → R in CAA49201 (PubMed:1427070).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69425 Genomic DNA. Translation: CAA49201.1.
M80302 Genomic DNA. Translation: AAA34765.1.
X97751 Genomic DNA. Translation: CAA66336.1.
Z74203 Genomic DNA. Translation: CAA98729.1.
AY692856 Genomic DNA. Translation: AAT92875.1.
BK006938 Genomic DNA. Translation: DAA11705.1.
PIRiA60048.
RefSeqiNP_010126.1. NM_001180215.1.

Genome annotation databases

EnsemblFungiiYDL155W; YDL155W; YDL155W.
GeneIDi851400.
KEGGisce:YDL155W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69425 Genomic DNA. Translation: CAA49201.1.
M80302 Genomic DNA. Translation: AAA34765.1.
X97751 Genomic DNA. Translation: CAA66336.1.
Z74203 Genomic DNA. Translation: CAA98729.1.
AY692856 Genomic DNA. Translation: AAT92875.1.
BK006938 Genomic DNA. Translation: DAA11705.1.
PIRiA60048.
RefSeqiNP_010126.1. NM_001180215.1.

3D structure databases

ProteinModelPortaliP24870.
SMRiP24870. Positions 166-411.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31908. 107 interactions.
DIPiDIP-1266N.
IntActiP24870. 31 interactions.
MINTiMINT-411020.

PTM databases

iPTMnetiP24870.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL155W; YDL155W; YDL155W.
GeneIDi851400.
KEGGisce:YDL155W.

Organism-specific databases

EuPathDBiFungiDB:YDL155W.
SGDiS000002314. CLB3.

Phylogenomic databases

GeneTreeiENSGT00760000118939.
HOGENOMiHOG000167672.
InParanoidiP24870.
KOiK06659.
OMAiYLLESTI.
OrthoDBiEOG7FNCJX.

Enzyme and pathway databases

BioCyciYEAST:G3O-29550-MONOMER.
ReactomeiR-SCE-113507. E2F-enabled inhibition of pre-replication complex formation.
R-SCE-113510. E2F mediated regulation of DNA replication.
R-SCE-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-SCE-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-SCE-3214858. RMTs methylate histone arginines.
R-SCE-4419969. Depolymerisation of the Nuclear Lamina.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-SCE-69202. Cyclin E associated events during G1/S transition.
R-SCE-69205. G1/S-Specific Transcription.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.
R-SCE-69478. G2/M DNA replication checkpoint.
R-SCE-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-SCE-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Miscellaneous databases

PROiP24870.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 2 hits.
SM01332. Cyclin_C. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cyclin-B homologs in Saccharomyces cerevisiae function in S phase and in G2."
    Richardson H., Lew D.J., Henze M., Sugimoto K., Reed S.I.
    Genes Dev. 6:2021-2034(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae."
    Fitch I., Dahmann C., Surana U., Amon A., Nasmyth K., Goetsch L., Byers B., Futcher B.
    Mol. Biol. Cell 3:805-818(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Analysis of a 23 kb region on the left arm of yeast chromosome IV."
    Delaveau T.T.D., Blugeon C., Jacq C., Perea J.
    Yeast 12:1587-1592(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae."
    Surana U., Robitsch H., Price C., Schuster T., Fitch I., Futcher A.B., Nasmyth K.
    Cell 65:145-161(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 194-383.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCG23_YEAST
AccessioniPrimary (citable) accession number: P24870
Secondary accession number(s): D6VRJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 25, 2004
Last modified: July 6, 2016
This is version 153 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 892 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.