P24869 (CG22_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G2/mitotic-specific cyclin-2

Gene names

Name:	CLB2
Ordered Locus Names:	YPR119W
ORF Names:	P9642.6

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the CDC2 protein kinase to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.
Subunit structure	Interacts with NAP1. Ref.5
Developmental stage	Maximally expressed before mitosis. The levels peak late in the G2 phase of the cell cycle and are at a minimum in G1 phase.
Miscellaneous	Present with 339 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the cyclin family. Cyclin AB subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Molecular function	Cyclin
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G2/M transition of mitotic cell cycle Inferred from expression pattern. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of G2/M transition of mitotic cell cycle Inferred from electronic annotation. Source: InterPro regulation of cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: InterPro
Cellular component	cellular bud neck Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD spindle pole body Inferred from direct assay. Source: SGD
Molecular function	protein kinase binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC28	P00546	3	EBI-4515,EBI-4253

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	G2/mitotic-specific cyclin-2		PRO_0000080404

Sequences

	Sequence		Length	Mass (Da)
	P24869 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: B68FF888871022A0	FASTA	491	56,247
	10 20 30 40 50 60 MSNPIENTEN SQNTSSSRFL RNVQRLALNN VTNTTFQKSN ANNPALTNFK STLNSVKKEG 70 80 90 100 110 120 SRIPQFTRES VSRSTAAQEE KRTLKENGIQ LPKNNLLDDK ENQDPSSQQF GALTSIKEGR 130 140 150 160 170 180 AELPANISLQ ESSSAKEIIQ HDPLKGVGSS TEVVHNSVEN EKLHPARSQL QVRNTESETD 190 200 210 220 230 240 SGKKRPISTI VEQELPKKFK VCDENGKEEY EWEDLDAEDV NDPFMVSEYV NDIFEYLHQL 250 260 270 280 290 300 EVITLPKKED LYQHRNIHQN RDILVNWLVK IHNKFGLLPE TLYLAINIMD RFLGKELVQL 310 320 330 340 350 360 DKLQLVGTSC LFIASKYEEV YSPSIKHFAS ETDGACTEDE IKEGEKFILK TLKFNLNYPN 370 380 390 400 410 420 PMNFLRRISK ADDYDIQSRT LAKFLLEISL VDFRFIGILP SLCAAAAMFM SRKMLGKGKW 430 440 450 460 470 480 DGNLIHYSGG YTKEELAPVC HMIMDYLVSP IVHDEFHRKY QSRRFMKASI ISVQWALKVR 490 KNGYDIMTLH E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae." Surana U., Robitsch H., Price C., Schuster T., Fitch I., Futcher A.B., Nasmyth K. Cell 65:145-161(1991) [PubMed: 1849457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cyclin-B homologs in Saccharomyces cerevisiae function in S phase and in G2." Richardson H., Lew D.J., Henze M., Sugimoto K., Reed S.I. Genes Dev. 6:2021-2034(1992) [PubMed: 1427070] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Members of the NAP/SET family of proteins interact specifically with B-type cyclins." Kellogg D.R., Kikuchi A., Fujii-Nakata T., Turck C.W., Murray A.W. J. Cell Biol. 130:661-673(1995) [PubMed: 7622566] [Abstract] Cited for: INTERACTION WTIH NAP1.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M65070 Genomic DNA. Translation: AAA34502.1. X62319 Genomic DNA. Translation: CAA44195.1. U40828 Genomic DNA. Translation: AAB68060.1. BK006949 Genomic DNA. Translation: DAA11534.1.
PIR	S14166.
RefSeq	NP_015444.1. NM_001184216.1.
3D structure databases
ProteinModelPortal	P24869.
SMR	P24869. Positions 226-472.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-658N.
IntAct	P24869. 21 interactions.
MINT	MINT-384557.
STRING	P24869.
Proteomic databases
PeptideAtlas	P24869.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR119W; YPR119W; YPR119W.
GeneID	856236.
KEGG	sce:YPR119W.
NMPDR	fig|4932.3.peg.6581.
Organism-specific databases
CYGD	YPR119w.
SGD	S000006323. CLB2.
Phylogenomic databases
eggNOG	fuNOG06119.
GeneTree	EFGT00050000003593.
HOGENOM	HBG324636.
OMA	EEYEWED.
OrthoDB	EOG4X9BRK.
Gene expression databases
ArrayExpress	P24869.
Genevestigator	P24869.
GermOnline	YPR119W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013763. Cyclin-like. IPR014400. Cyclin_A/B/D/E. IPR015454. Cyclin_B. IPR004367. Cyclin_C. IPR006671. Cyclin_N. [Graphical view]
Gene3D	G3DSA:1.10.472.10. Cyclin_related. 2 hits.
KO	K02220.
PANTHER	PTHR10177:SF27. CycB. 1 hit.
Pfam	PF02984. Cyclin_C. 1 hit. PF00134. Cyclin_N. 1 hit. [Graphical view]
PIRSF	PIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMART	SM00385. CYCLIN. 2 hits. [Graphical view]
SUPFAM	SSF47954. Cyclin_like. 2 hits.
PROSITE	PS00292. CYCLINS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981493.

Entry information

Entry name

CG22_YEAST

Accession

Primary (citable) accession number: P24869
Secondary accession number(s): D6W4B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	December 14, 2011
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program