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Protein

G2/mitotic-specific cyclin-2

Gene

CLB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the CDC2 protein kinase to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.

GO - Molecular functioni

  • cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • G2/M transition of mitotic cell cycle Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • negative regulation of protein dephosphorylation Source: SGD
  • positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD
  • positive regulation of spindle pole body separation Source: SGD
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD
  • regulation of mitotic spindle elongation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-34258-MONOMER.
ReactomeiR-SCE-113507. E2F-enabled inhibition of pre-replication complex formation.
R-SCE-113510. E2F mediated regulation of DNA replication.
R-SCE-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-SCE-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-SCE-3214858. RMTs methylate histone arginines.
R-SCE-4419969. Depolymerisation of the Nuclear Lamina.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-5689880. Ub-specific processing proteases.
R-SCE-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-69202. Cyclin E associated events during G1/S transition.
R-SCE-69205. G1/S-Specific Transcription.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.
R-SCE-69478. G2/M DNA replication checkpoint.
R-SCE-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-SCE-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
G2/mitotic-specific cyclin-2
Gene namesi
Name:CLB2
Ordered Locus Names:YPR119W
ORF Names:P9642.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR119W.
SGDiS000006323. CLB2.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cytoplasm Source: SGD
  • nucleus Source: SGD
  • spindle Source: SGD
  • spindle pole body Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000804042 – 491G2/mitotic-specific cyclin-2Add BLAST490

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP24869.
PRIDEiP24869.

PTM databases

iPTMnetiP24869.

Expressioni

Developmental stagei

Maximally expressed before mitosis. The levels peak late in the G2 phase of the cell cycle and are at a minimum in G1 phase.

Interactioni

Subunit structurei

Interacts with NAP1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ASK1P357342EBI-4515,EBI-26682
FIN1Q038982EBI-4515,EBI-32941
ORC6P388262EBI-4515,EBI-12588
SLI15P382832EBI-4515,EBI-20842

Protein-protein interaction databases

BioGridi36287. 214 interactors.
DIPiDIP-658N.
IntActiP24869. 24 interactors.
MINTiMINT-384557.

Structurei

3D structure databases

ProteinModelPortaliP24869.
SMRiP24869.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family. Cyclin AB subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00760000118939.
HOGENOMiHOG000167672.
InParanoidiP24869.
KOiK02220.
OMAiEEYEWED.
OrthoDBiEOG092C2S0G.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 2 hits.
SM01332. Cyclin_C. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24869-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNPIENTEN SQNTSSSRFL RNVQRLALNN VTNTTFQKSN ANNPALTNFK
60 70 80 90 100
STLNSVKKEG SRIPQFTRES VSRSTAAQEE KRTLKENGIQ LPKNNLLDDK
110 120 130 140 150
ENQDPSSQQF GALTSIKEGR AELPANISLQ ESSSAKEIIQ HDPLKGVGSS
160 170 180 190 200
TEVVHNSVEN EKLHPARSQL QVRNTESETD SGKKRPISTI VEQELPKKFK
210 220 230 240 250
VCDENGKEEY EWEDLDAEDV NDPFMVSEYV NDIFEYLHQL EVITLPKKED
260 270 280 290 300
LYQHRNIHQN RDILVNWLVK IHNKFGLLPE TLYLAINIMD RFLGKELVQL
310 320 330 340 350
DKLQLVGTSC LFIASKYEEV YSPSIKHFAS ETDGACTEDE IKEGEKFILK
360 370 380 390 400
TLKFNLNYPN PMNFLRRISK ADDYDIQSRT LAKFLLEISL VDFRFIGILP
410 420 430 440 450
SLCAAAAMFM SRKMLGKGKW DGNLIHYSGG YTKEELAPVC HMIMDYLVSP
460 470 480 490
IVHDEFHRKY QSRRFMKASI ISVQWALKVR KNGYDIMTLH E
Length:491
Mass (Da):56,247
Last modified:March 1, 1992 - v1
Checksum:iB68FF888871022A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65070 Genomic DNA. Translation: AAA34502.1.
X62319 Genomic DNA. Translation: CAA44195.1.
U40828 Genomic DNA. Translation: AAB68060.1.
BK006949 Genomic DNA. Translation: DAA11534.1.
PIRiS14166.
RefSeqiNP_015444.1. NM_001184216.1.

Genome annotation databases

EnsemblFungiiYPR119W; YPR119W; YPR119W.
GeneIDi856236.
KEGGisce:YPR119W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65070 Genomic DNA. Translation: AAA34502.1.
X62319 Genomic DNA. Translation: CAA44195.1.
U40828 Genomic DNA. Translation: AAB68060.1.
BK006949 Genomic DNA. Translation: DAA11534.1.
PIRiS14166.
RefSeqiNP_015444.1. NM_001184216.1.

3D structure databases

ProteinModelPortaliP24869.
SMRiP24869.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36287. 214 interactors.
DIPiDIP-658N.
IntActiP24869. 24 interactors.
MINTiMINT-384557.

PTM databases

iPTMnetiP24869.

Proteomic databases

MaxQBiP24869.
PRIDEiP24869.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR119W; YPR119W; YPR119W.
GeneIDi856236.
KEGGisce:YPR119W.

Organism-specific databases

EuPathDBiFungiDB:YPR119W.
SGDiS000006323. CLB2.

Phylogenomic databases

GeneTreeiENSGT00760000118939.
HOGENOMiHOG000167672.
InParanoidiP24869.
KOiK02220.
OMAiEEYEWED.
OrthoDBiEOG092C2S0G.

Enzyme and pathway databases

BioCyciYEAST:G3O-34258-MONOMER.
ReactomeiR-SCE-113507. E2F-enabled inhibition of pre-replication complex formation.
R-SCE-113510. E2F mediated regulation of DNA replication.
R-SCE-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-SCE-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-SCE-3214858. RMTs methylate histone arginines.
R-SCE-4419969. Depolymerisation of the Nuclear Lamina.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-5689880. Ub-specific processing proteases.
R-SCE-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-69202. Cyclin E associated events during G1/S transition.
R-SCE-69205. G1/S-Specific Transcription.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.
R-SCE-69478. G2/M DNA replication checkpoint.
R-SCE-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-SCE-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Miscellaneous databases

PROiP24869.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 2 hits.
SM01332. Cyclin_C. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCG22_YEAST
AccessioniPrimary (citable) accession number: P24869
Secondary accession number(s): D6W4B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 30, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 339 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.