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P24869

- CG22_YEAST

UniProt

P24869 - CG22_YEAST

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Protein

G2/mitotic-specific cyclin-2

Gene

CLB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the CDC2 protein kinase to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD

GO - Biological processi

  1. G2/M transition of mitotic cell cycle Source: SGD
  2. mitotic nuclear division Source: UniProtKB-KW
  3. negative regulation of protein dephosphorylation Source: SGD
  4. positive regulation of spindle pole body separation Source: SGD
  5. regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD
  6. regulation of mitotic spindle elongation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-34258-MONOMER.
ReactomeiREACT_188466. Regulation of APC/C activators between G1/S and early anaphase.
REACT_188487. Cyclin A/B1 associated events during G2/M transition.
REACT_188488. G2/M DNA replication checkpoint.
REACT_188908. Condensation of Prometaphase Chromosomes.
REACT_188916. MASTL Facilitates Mitotic Progression.
REACT_205363. Cyclin B2 mediated events.
REACT_211772. E2F-enabled inhibition of pre-replication complex formation.
REACT_224510. Depolymerisation of the Nuclear Lamina.

Names & Taxonomyi

Protein namesi
Recommended name:
G2/mitotic-specific cyclin-2
Gene namesi
Name:CLB2
Ordered Locus Names:YPR119W
ORF Names:P9642.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR119w.
SGDiS000006323. CLB2.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cytoplasm Source: SGD
  3. nucleus Source: SGD
  4. spindle Source: SGD
  5. spindle pole body Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 491490G2/mitotic-specific cyclin-2PRO_0000080404Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP24869.
PaxDbiP24869.
PeptideAtlasiP24869.

Expressioni

Developmental stagei

Maximally expressed before mitosis. The levels peak late in the G2 phase of the cell cycle and are at a minimum in G1 phase.

Gene expression databases

GenevestigatoriP24869.

Interactioni

Subunit structurei

Interacts with NAP1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ASK1P357342EBI-4515,EBI-26682
FIN1Q038982EBI-4515,EBI-32941
ORC6P388262EBI-4515,EBI-12588
SLI15P382832EBI-4515,EBI-20842

Protein-protein interaction databases

BioGridi36287. 211 interactions.
DIPiDIP-658N.
IntActiP24869. 24 interactions.
MINTiMINT-384557.
STRINGi4932.YPR119W.

Structurei

3D structure databases

ProteinModelPortaliP24869.
SMRiP24869. Positions 227-471.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family. Cyclin AB subfamily.Curated

Phylogenomic databases

eggNOGiCOG5024.
GeneTreeiENSGT00760000118939.
HOGENOMiHOG000167672.
InParanoidiP24869.
KOiK02220.
OMAiEEYEWED.
OrthoDBiEOG7FNCJX.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR014400. Cyclin_A/B/D/E/F.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24869-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNPIENTEN SQNTSSSRFL RNVQRLALNN VTNTTFQKSN ANNPALTNFK
60 70 80 90 100
STLNSVKKEG SRIPQFTRES VSRSTAAQEE KRTLKENGIQ LPKNNLLDDK
110 120 130 140 150
ENQDPSSQQF GALTSIKEGR AELPANISLQ ESSSAKEIIQ HDPLKGVGSS
160 170 180 190 200
TEVVHNSVEN EKLHPARSQL QVRNTESETD SGKKRPISTI VEQELPKKFK
210 220 230 240 250
VCDENGKEEY EWEDLDAEDV NDPFMVSEYV NDIFEYLHQL EVITLPKKED
260 270 280 290 300
LYQHRNIHQN RDILVNWLVK IHNKFGLLPE TLYLAINIMD RFLGKELVQL
310 320 330 340 350
DKLQLVGTSC LFIASKYEEV YSPSIKHFAS ETDGACTEDE IKEGEKFILK
360 370 380 390 400
TLKFNLNYPN PMNFLRRISK ADDYDIQSRT LAKFLLEISL VDFRFIGILP
410 420 430 440 450
SLCAAAAMFM SRKMLGKGKW DGNLIHYSGG YTKEELAPVC HMIMDYLVSP
460 470 480 490
IVHDEFHRKY QSRRFMKASI ISVQWALKVR KNGYDIMTLH E
Length:491
Mass (Da):56,247
Last modified:March 1, 1992 - v1
Checksum:iB68FF888871022A0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M65070 Genomic DNA. Translation: AAA34502.1.
X62319 Genomic DNA. Translation: CAA44195.1.
U40828 Genomic DNA. Translation: AAB68060.1.
BK006949 Genomic DNA. Translation: DAA11534.1.
PIRiS14166.
RefSeqiNP_015444.1. NM_001184216.1.

Genome annotation databases

EnsemblFungiiYPR119W; YPR119W; YPR119W.
GeneIDi856236.
KEGGisce:YPR119W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M65070 Genomic DNA. Translation: AAA34502.1 .
X62319 Genomic DNA. Translation: CAA44195.1 .
U40828 Genomic DNA. Translation: AAB68060.1 .
BK006949 Genomic DNA. Translation: DAA11534.1 .
PIRi S14166.
RefSeqi NP_015444.1. NM_001184216.1.

3D structure databases

ProteinModelPortali P24869.
SMRi P24869. Positions 227-471.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36287. 211 interactions.
DIPi DIP-658N.
IntActi P24869. 24 interactions.
MINTi MINT-384557.
STRINGi 4932.YPR119W.

Proteomic databases

MaxQBi P24869.
PaxDbi P24869.
PeptideAtlasi P24869.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR119W ; YPR119W ; YPR119W .
GeneIDi 856236.
KEGGi sce:YPR119W.

Organism-specific databases

CYGDi YPR119w.
SGDi S000006323. CLB2.

Phylogenomic databases

eggNOGi COG5024.
GeneTreei ENSGT00760000118939.
HOGENOMi HOG000167672.
InParanoidi P24869.
KOi K02220.
OMAi EEYEWED.
OrthoDBi EOG7FNCJX.

Enzyme and pathway databases

BioCyci YEAST:G3O-34258-MONOMER.
Reactomei REACT_188466. Regulation of APC/C activators between G1/S and early anaphase.
REACT_188487. Cyclin A/B1 associated events during G2/M transition.
REACT_188488. G2/M DNA replication checkpoint.
REACT_188908. Condensation of Prometaphase Chromosomes.
REACT_188916. MASTL Facilitates Mitotic Progression.
REACT_205363. Cyclin B2 mediated events.
REACT_211772. E2F-enabled inhibition of pre-replication complex formation.
REACT_224510. Depolymerisation of the Nuclear Lamina.

Miscellaneous databases

NextBioi 981493.

Gene expression databases

Genevestigatori P24869.

Family and domain databases

Gene3Di 1.10.472.10. 2 hits.
InterProi IPR013763. Cyclin-like.
IPR014400. Cyclin_A/B/D/E/F.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view ]
Pfami PF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMARTi SM00385. CYCLIN. 2 hits.
[Graphical view ]
SUPFAMi SSF47954. SSF47954. 2 hits.
PROSITEi PS00292. CYCLINS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae."
    Surana U., Robitsch H., Price C., Schuster T., Fitch I., Futcher A.B., Nasmyth K.
    Cell 65:145-161(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cyclin-B homologs in Saccharomyces cerevisiae function in S phase and in G2."
    Richardson H., Lew D.J., Henze M., Sugimoto K., Reed S.I.
    Genes Dev. 6:2021-2034(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Members of the NAP/SET family of proteins interact specifically with B-type cyclins."
    Kellogg D.R., Kikuchi A., Fujii-Nakata T., Turck C.W., Murray A.W.
    J. Cell Biol. 130:661-673(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NAP1.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCG22_YEAST
AccessioniPrimary (citable) accession number: P24869
Secondary accession number(s): D6W4B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: October 29, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 339 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3