Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P24869

- CG22_YEAST

UniProt

P24869 - CG22_YEAST

Protein

G2/mitotic-specific cyclin-2

Gene

CLB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the CDC2 protein kinase to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.

    GO - Molecular functioni

    1. cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. G2/M transition of mitotic cell cycle Source: SGD
    2. mitotic nuclear division Source: UniProtKB-KW
    3. negative regulation of protein dephosphorylation Source: SGD
    4. positive regulation of spindle pole body separation Source: SGD
    5. regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD
    6. regulation of mitotic spindle elongation Source: SGD

    Keywords - Molecular functioni

    Cyclin

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34258-MONOMER.
    ReactomeiREACT_188466. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_188487. Cyclin A/B1 associated events during G2/M transition.
    REACT_188488. G2/M DNA replication checkpoint.
    REACT_188908. Condensation of Prometaphase Chromosomes.
    REACT_188916. MASTL Facilitates Mitotic Progression.
    REACT_205363. Cyclin B2 mediated events.
    REACT_211772. E2F-enabled inhibition of pre-replication complex formation.
    REACT_224510. Depolymerisation of the Nuclear Lamina.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G2/mitotic-specific cyclin-2
    Gene namesi
    Name:CLB2
    Ordered Locus Names:YPR119W
    ORF Names:P9642.6
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR119w.
    SGDiS000006323. CLB2.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cytoplasm Source: SGD
    3. nucleus Source: SGD
    4. spindle Source: SGD
    5. spindle pole body Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 491490G2/mitotic-specific cyclin-2PRO_0000080404Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP24869.
    PaxDbiP24869.
    PeptideAtlasiP24869.

    Expressioni

    Developmental stagei

    Maximally expressed before mitosis. The levels peak late in the G2 phase of the cell cycle and are at a minimum in G1 phase.

    Gene expression databases

    GenevestigatoriP24869.

    Interactioni

    Subunit structurei

    Interacts with NAP1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ASK1P357342EBI-4515,EBI-26682
    FIN1Q038982EBI-4515,EBI-32941
    ORC6P388262EBI-4515,EBI-12588
    SLI15P382832EBI-4515,EBI-20842

    Protein-protein interaction databases

    BioGridi36287. 211 interactions.
    DIPiDIP-658N.
    IntActiP24869. 24 interactions.
    MINTiMINT-384557.
    STRINGi4932.YPR119W.

    Structurei

    3D structure databases

    ProteinModelPortaliP24869.
    SMRiP24869. Positions 227-471.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cyclin family. Cyclin AB subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG5024.
    GeneTreeiENSGT00750000117276.
    HOGENOMiHOG000167672.
    KOiK02220.
    OMAiEEYEWED.
    OrthoDBiEOG7FNCJX.

    Family and domain databases

    Gene3Di1.10.472.10. 2 hits.
    InterProiIPR013763. Cyclin-like.
    IPR014400. Cyclin_A/B/D/E/F.
    IPR004367. Cyclin_C-dom.
    IPR006671. Cyclin_N.
    [Graphical view]
    PfamiPF02984. Cyclin_C. 1 hit.
    PF00134. Cyclin_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001771. Cyclin_A_B_D_E. 1 hit.
    SMARTiSM00385. CYCLIN. 2 hits.
    [Graphical view]
    SUPFAMiSSF47954. SSF47954. 2 hits.
    PROSITEiPS00292. CYCLINS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24869-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNPIENTEN SQNTSSSRFL RNVQRLALNN VTNTTFQKSN ANNPALTNFK    50
    STLNSVKKEG SRIPQFTRES VSRSTAAQEE KRTLKENGIQ LPKNNLLDDK 100
    ENQDPSSQQF GALTSIKEGR AELPANISLQ ESSSAKEIIQ HDPLKGVGSS 150
    TEVVHNSVEN EKLHPARSQL QVRNTESETD SGKKRPISTI VEQELPKKFK 200
    VCDENGKEEY EWEDLDAEDV NDPFMVSEYV NDIFEYLHQL EVITLPKKED 250
    LYQHRNIHQN RDILVNWLVK IHNKFGLLPE TLYLAINIMD RFLGKELVQL 300
    DKLQLVGTSC LFIASKYEEV YSPSIKHFAS ETDGACTEDE IKEGEKFILK 350
    TLKFNLNYPN PMNFLRRISK ADDYDIQSRT LAKFLLEISL VDFRFIGILP 400
    SLCAAAAMFM SRKMLGKGKW DGNLIHYSGG YTKEELAPVC HMIMDYLVSP 450
    IVHDEFHRKY QSRRFMKASI ISVQWALKVR KNGYDIMTLH E 491
    Length:491
    Mass (Da):56,247
    Last modified:March 1, 1992 - v1
    Checksum:iB68FF888871022A0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M65070 Genomic DNA. Translation: AAA34502.1.
    X62319 Genomic DNA. Translation: CAA44195.1.
    U40828 Genomic DNA. Translation: AAB68060.1.
    BK006949 Genomic DNA. Translation: DAA11534.1.
    PIRiS14166.
    RefSeqiNP_015444.1. NM_001184216.1.

    Genome annotation databases

    EnsemblFungiiYPR119W; YPR119W; YPR119W.
    GeneIDi856236.
    KEGGisce:YPR119W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M65070 Genomic DNA. Translation: AAA34502.1 .
    X62319 Genomic DNA. Translation: CAA44195.1 .
    U40828 Genomic DNA. Translation: AAB68060.1 .
    BK006949 Genomic DNA. Translation: DAA11534.1 .
    PIRi S14166.
    RefSeqi NP_015444.1. NM_001184216.1.

    3D structure databases

    ProteinModelPortali P24869.
    SMRi P24869. Positions 227-471.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36287. 211 interactions.
    DIPi DIP-658N.
    IntActi P24869. 24 interactions.
    MINTi MINT-384557.
    STRINGi 4932.YPR119W.

    Proteomic databases

    MaxQBi P24869.
    PaxDbi P24869.
    PeptideAtlasi P24869.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR119W ; YPR119W ; YPR119W .
    GeneIDi 856236.
    KEGGi sce:YPR119W.

    Organism-specific databases

    CYGDi YPR119w.
    SGDi S000006323. CLB2.

    Phylogenomic databases

    eggNOGi COG5024.
    GeneTreei ENSGT00750000117276.
    HOGENOMi HOG000167672.
    KOi K02220.
    OMAi EEYEWED.
    OrthoDBi EOG7FNCJX.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34258-MONOMER.
    Reactomei REACT_188466. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_188487. Cyclin A/B1 associated events during G2/M transition.
    REACT_188488. G2/M DNA replication checkpoint.
    REACT_188908. Condensation of Prometaphase Chromosomes.
    REACT_188916. MASTL Facilitates Mitotic Progression.
    REACT_205363. Cyclin B2 mediated events.
    REACT_211772. E2F-enabled inhibition of pre-replication complex formation.
    REACT_224510. Depolymerisation of the Nuclear Lamina.

    Miscellaneous databases

    NextBioi 981493.

    Gene expression databases

    Genevestigatori P24869.

    Family and domain databases

    Gene3Di 1.10.472.10. 2 hits.
    InterProi IPR013763. Cyclin-like.
    IPR014400. Cyclin_A/B/D/E/F.
    IPR004367. Cyclin_C-dom.
    IPR006671. Cyclin_N.
    [Graphical view ]
    Pfami PF02984. Cyclin_C. 1 hit.
    PF00134. Cyclin_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001771. Cyclin_A_B_D_E. 1 hit.
    SMARTi SM00385. CYCLIN. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47954. SSF47954. 2 hits.
    PROSITEi PS00292. CYCLINS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae."
      Surana U., Robitsch H., Price C., Schuster T., Fitch I., Futcher A.B., Nasmyth K.
      Cell 65:145-161(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cyclin-B homologs in Saccharomyces cerevisiae function in S phase and in G2."
      Richardson H., Lew D.J., Henze M., Sugimoto K., Reed S.I.
      Genes Dev. 6:2021-2034(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Members of the NAP/SET family of proteins interact specifically with B-type cyclins."
      Kellogg D.R., Kikuchi A., Fujii-Nakata T., Turck C.W., Murray A.W.
      J. Cell Biol. 130:661-673(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NAP1.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCG22_YEAST
    AccessioniPrimary (citable) accession number: P24869
    Secondary accession number(s): D6W4B8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 339 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3