P24868 (CG21_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G2/mitotic-specific cyclin-1

Gene names

Name:	CLB1
Synonyms:	SCB1
Ordered Locus Names:	YGR108W
ORF Names:	G5967

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the CDC2 protein kinase to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.
Developmental stage	Maximally expressed before mitosis. The levels peak late in the G2 phase of the cell cycle and are at a minimum in G1 phase.
Miscellaneous	Present with 300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the cyclin family. Cyclin AB subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Molecular function	Cyclin
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G2/M transition of mitotic cell cycle Inferred from genetic interaction PubMed 1427070. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW meiotic G2/MI transition Inferred from expression pattern PubMed 8455600. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic spindle organization in nucleus Inferred from mutant phenotype PubMed 1427070. Source: SGD positive regulation of spindle pole body separation Inferred from genetic interaction PubMed 1387566. Source: SGD regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from mutant phenotype Ref.2. Source: SGD
Cellular_component	cyclin-dependent protein kinase holoenzyme complex Inferred from direct assay Ref.2. Source: SGD cytoplasm Inferred from direct assay PubMed 12972503. Source: SGD nucleus Inferred from direct assay PubMed 12972503PubMed 14562095. Source: SGD
Molecular_function	cyclin-dependent protein serine/threonine kinase regulator activity Inferred from mutant phenotype Ref.2. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CKS1	P20486	3	EBI-4508,EBI-4746

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	G2/mitotic-specific cyclin-1		PRO_0000080403

Sequences

	Sequence		Length	Mass (Da)
	P24868 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 4B347E96DD188735	FASTA	471	54,871
	10 20 30 40 50 60 MSRSLLVENS RTINSNEEKG VNESQYILQK RNVPRTILGN VTNNANILQE ISMNRKIGMK 70 80 90 100 110 120 NFSKLNNFFP LKDDVSRADD FTSSFNDSRQ GVKQEVLNNK ENIPEYGYSE QEKQQCSNDD 130 140 150 160 170 180 SFHTNSTALS CNRLIYSENK SISTQMEWQK KIMREDSKKK RPISTLVEQD DQKKFKLHEL 190 200 210 220 230 240 TTEEEVLEEY EWDDLDEEDC DDPLMVSEEV NDIFDYLHHL EIITLPNKAN LYKHKNIKQN 250 260 270 280 290 300 RDILVNWIIK IHNKFGLLPE TLYLAINIMD RFLCEEVVQL NRLQLVGTSC LFIASKYEEI 310 320 330 340 350 360 YSPSIKHFAY ETDGACSVED IKEGERFILE KLDFQISFAN PMNFLRRISK ADDYDIQSRT 370 380 390 400 410 420 LAKFLMEISI VDFKFIGILP SLCASAAMFL SRKMLGKGTW DGNLIHYSGG YTKAKLYPVC 430 440 450 460 470 QLLMDYLVGS TIHDEFLKKY QSRRFLKASI ISIEWALKVR KNGYDIMTLH E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae." Surana U., Robitsch H., Price C., Schuster T., Fitch I., Futcher A.B., Nasmyth K. Cell 65:145-161(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis." Ghiara J.B., Richardson H.E., Sugimoto K., Henze M., Lew D.J., Wittenberg C., Reed S.I. Cell 65:163-174(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M65069 Genomic DNA. Translation: AAA34501.1. M62389 Genomic DNA. Translation: AAA35019.1. Z72893 Genomic DNA. Translation: CAA97112.1. BK006941 Genomic DNA. Translation: DAA08200.1.
PIR	S14165.
RefSeq	NP_011622.1. NM_001181237.1.
3D structure databases
ProteinModelPortal	P24868.
SMR	P24868. Positions 207-451.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1261N.
IntAct	P24868. 5 interactions.
MINT	MINT-410951.
STRING	4932.YGR108W.
Proteomic databases
PaxDb	P24868.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR108W; YGR108W; YGR108W.
GeneID	853002.
KEGG	sce:YGR108W.
Organism-specific databases
CYGD	YGR108w.
SGD	S000003340. CLB1.
Phylogenomic databases
eggNOG	COG5024.
GeneTree	ENSGT00560000076692.
HOGENOM	HOG000167672.
KO	K02220.
OrthoDB	EOG4X9BRK.
Gene expression databases
Genevestigator	P24868.
GermOnline	YGR108W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.472.10. 2 hits.
InterPro	IPR013763. Cyclin-like. IPR014400. Cyclin_A/B/D/E. IPR004367. Cyclin_C-dom. IPR006671. Cyclin_N. [Graphical view]
Pfam	PF02984. Cyclin_C. 1 hit. PF00134. Cyclin_N. 1 hit. [Graphical view]
PIRSF	PIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMART	SM00385. CYCLIN. 2 hits. [Graphical view]
SUPFAM	SSF47954. Cyclin_like. 2 hits.
PROSITE	PS00292. CYCLINS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972846.

Entry information

Entry name

CG21_YEAST

Accession

Primary (citable) accession number: P24868
Secondary accession number(s): D6VUN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program