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Protein

G2/mitotic-specific cyclin-1

Gene

CLB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the CDC2 protein kinase to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.

GO - Molecular functioni

  • cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • G2/M transition of mitotic cell cycle Source: SGD
  • meiotic G2/MI transition Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • mitotic spindle organization in nucleus Source: SGD
  • positive regulation of spindle pole body separation Source: SGD
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30817-MONOMER.
ReactomeiREACT_276885. Cyclin B2 mediated events.
REACT_284037. Condensation of Prometaphase Chromosomes.
REACT_292147. Regulation of APC/C activators between G1/S and early anaphase.
REACT_293633. E2F-enabled inhibition of pre-replication complex formation.
REACT_305381. Cyclin A/B1 associated events during G2/M transition.
REACT_324018. Depolymerisation of the Nuclear Lamina.
REACT_354841. G2/M DNA replication checkpoint.

Names & Taxonomyi

Protein namesi
Recommended name:
G2/mitotic-specific cyclin-1
Gene namesi
Name:CLB1
Synonyms:SCB1
Ordered Locus Names:YGR108W
ORF Names:G5967
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

CYGDiYGR108w.
EuPathDBiFungiDB:YGR108W.
SGDiS000003340. CLB1.

Subcellular locationi

GO - Cellular componenti

  • cyclin-dependent protein kinase holoenzyme complex Source: SGD
  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471G2/mitotic-specific cyclin-1PRO_0000080403Add
BLAST

Proteomic databases

PaxDbiP24868.

Expressioni

Developmental stagei

Maximally expressed before mitosis. The levels peak late in the G2 phase of the cell cycle and are at a minimum in G1 phase.

Gene expression databases

GenevestigatoriP24868.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CKS1P204863EBI-4508,EBI-4746

Protein-protein interaction databases

BioGridi33352. 68 interactions.
DIPiDIP-1261N.
IntActiP24868. 5 interactions.
MINTiMINT-410951.
STRINGi4932.YGR108W.

Structurei

3D structure databases

ProteinModelPortaliP24868.
SMRiP24868. Positions 207-451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family. Cyclin AB subfamily.Curated

Phylogenomic databases

eggNOGiCOG5024.
GeneTreeiENSGT00760000118939.
HOGENOMiHOG000167672.
InParanoidiP24868.
KOiK02220.
OrthoDBiEOG7FNCJX.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24868-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRSLLVENS RTINSNEEKG VNESQYILQK RNVPRTILGN VTNNANILQE
60 70 80 90 100
ISMNRKIGMK NFSKLNNFFP LKDDVSRADD FTSSFNDSRQ GVKQEVLNNK
110 120 130 140 150
ENIPEYGYSE QEKQQCSNDD SFHTNSTALS CNRLIYSENK SISTQMEWQK
160 170 180 190 200
KIMREDSKKK RPISTLVEQD DQKKFKLHEL TTEEEVLEEY EWDDLDEEDC
210 220 230 240 250
DDPLMVSEEV NDIFDYLHHL EIITLPNKAN LYKHKNIKQN RDILVNWIIK
260 270 280 290 300
IHNKFGLLPE TLYLAINIMD RFLCEEVVQL NRLQLVGTSC LFIASKYEEI
310 320 330 340 350
YSPSIKHFAY ETDGACSVED IKEGERFILE KLDFQISFAN PMNFLRRISK
360 370 380 390 400
ADDYDIQSRT LAKFLMEISI VDFKFIGILP SLCASAAMFL SRKMLGKGTW
410 420 430 440 450
DGNLIHYSGG YTKAKLYPVC QLLMDYLVGS TIHDEFLKKY QSRRFLKASI
460 470
ISIEWALKVR KNGYDIMTLH E
Length:471
Mass (Da):54,871
Last modified:March 1, 1992 - v1
Checksum:i4B347E96DD188735
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65069 Genomic DNA. Translation: AAA34501.1.
M62389 Genomic DNA. Translation: AAA35019.1.
Z72893 Genomic DNA. Translation: CAA97112.1.
BK006941 Genomic DNA. Translation: DAA08200.1.
PIRiS14165.
RefSeqiNP_011622.1. NM_001181237.1.

Genome annotation databases

EnsemblFungiiYGR108W; YGR108W; YGR108W.
GeneIDi853002.
KEGGisce:YGR108W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65069 Genomic DNA. Translation: AAA34501.1.
M62389 Genomic DNA. Translation: AAA35019.1.
Z72893 Genomic DNA. Translation: CAA97112.1.
BK006941 Genomic DNA. Translation: DAA08200.1.
PIRiS14165.
RefSeqiNP_011622.1. NM_001181237.1.

3D structure databases

ProteinModelPortaliP24868.
SMRiP24868. Positions 207-451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33352. 68 interactions.
DIPiDIP-1261N.
IntActiP24868. 5 interactions.
MINTiMINT-410951.
STRINGi4932.YGR108W.

Proteomic databases

PaxDbiP24868.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR108W; YGR108W; YGR108W.
GeneIDi853002.
KEGGisce:YGR108W.

Organism-specific databases

CYGDiYGR108w.
EuPathDBiFungiDB:YGR108W.
SGDiS000003340. CLB1.

Phylogenomic databases

eggNOGiCOG5024.
GeneTreeiENSGT00760000118939.
HOGENOMiHOG000167672.
InParanoidiP24868.
KOiK02220.
OrthoDBiEOG7FNCJX.

Enzyme and pathway databases

BioCyciYEAST:G3O-30817-MONOMER.
ReactomeiREACT_276885. Cyclin B2 mediated events.
REACT_284037. Condensation of Prometaphase Chromosomes.
REACT_292147. Regulation of APC/C activators between G1/S and early anaphase.
REACT_293633. E2F-enabled inhibition of pre-replication complex formation.
REACT_305381. Cyclin A/B1 associated events during G2/M transition.
REACT_324018. Depolymerisation of the Nuclear Lamina.
REACT_354841. G2/M DNA replication checkpoint.

Miscellaneous databases

NextBioi972846.
PROiP24868.

Gene expression databases

GenevestigatoriP24868.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae."
    Surana U., Robitsch H., Price C., Schuster T., Fitch I., Futcher A.B., Nasmyth K.
    Cell 65:145-161(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis."
    Ghiara J.B., Richardson H.E., Sugimoto K., Henze M., Lew D.J., Wittenberg C., Reed S.I.
    Cell 65:163-174(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCG21_YEAST
AccessioniPrimary (citable) accession number: P24868
Secondary accession number(s): D6VUN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: May 27, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.