P24847 (DAPA2_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Dihydrodipicolinate synthase 2, chloroplastic Short name=DHDPS 2 EC=4.2.1.52
Organism	Triticum aestivum (Wheat)
Taxonomic identifier	4565 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O.
Enzyme regulation	Sensitive to lysine inhibition. This inhibition increase in an allosteric manner with increasing concentration of the inhibitor.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.
Subunit structure	Tetramer of modified subunits derived from two genes in different combinations.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Schiff base
Molecular function	Lyase
Technical term	Allosteric enzyme Direct protein sequencing
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 51	51	Chloroplast Ref.1
	Chain	52 – 377	326	Dihydrodipicolinate synthase 2, chloroplastic		PRO_0000007204
Sites
	Active site	234	1	Schiff-base intermediate with substrate By similarity
	Binding site	181	1	Pyruvate By similarity
	Site	206	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24847 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: B0C08FC482BA6CDC	FASTA	377	40,876
	10 20 30 40 50 60 MMAAQPTANP GVRLGWKAPG ALASPPRLAL SRSAAAPLAS HRVGRGKFSA AAITTDDYLP 70 80 90 100 110 120 MRSTEVKNRT SVDGIKSLRL ITAVKTPYLP DGRFDLEAYD SLINTQINGG AEGVIVGGTT 130 140 150 160 170 180 GEGHLMSWDE HIMLIGHTVN CFGTNIKVIG NTGSNSTREA IHASEQGFAV GMHAALHVNP 190 200 210 220 230 240 YYGKTSTAGL ISHFDEVLPM GPTIIYNVPS RTGQDIPPAV IEALSTYPNM AGVKECVGHE 250 260 270 280 290 300 RVKCYTDKGI TIWSGNDDEC HDSRWKYGAT GVISVTSNLV PGLMRSLMFE GENAALNEKL 310 320 330 340 350 360 LPLMKWLFSE PNPIGLNTAL AQLGVVRPVF RRPYAPLSLE KRTEFVRIVE AIGRENFVGQ 370 KEVRVLDDDD FVLISRY

References

[1]	"Molecular cloning of wheat dihydrodipicolinate synthase." Kaneko T., Hashimoto T., Kumpaisal R., Yamada Y. J. Biol. Chem. 265:17451-17455(1990) [PubMed: 2211639] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 52-68. Strain: cv. Chinese Spring.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60599 mRNA. Translation: AAA34264.1.
PIR	WZWTH6. B39213.
UniGene	Ta.152.
3D structure databases
ProteinModelPortal	P24847.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P24847.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA2_WHEAT

Accession

Primary (citable) accession number: P24847

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	September 21, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program