Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P24844

- MYL9_HUMAN

UniProt

P24844 - MYL9_HUMAN

Protein

Myosin regulatory light polypeptide 9

Gene

MYL9

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi42 – 5312Add
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. structural constituent of muscle Source: ProtInc

    GO - Biological processi

    1. axon guidance Source: Reactome
    2. muscle contraction Source: Reactome
    3. regulation of muscle contraction Source: ProtInc

    Keywords - Molecular functioni

    Motor protein, Muscle protein, Myosin

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_19277. Sema4D induced cell migration and growth-cone collapse.
    REACT_20558. Smooth Muscle Contraction.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin regulatory light polypeptide 9
    Alternative name(s):
    20 kDa myosin light chain
    Short name:
    LC20
    MLC-2C
    Myosin RLC
    Myosin regulatory light chain 2, smooth muscle isoform
    Myosin regulatory light chain 9
    Myosin regulatory light chain MRLC1
    Gene namesi
    Name:MYL9
    Synonyms:MLC2, MRLC1, MYRL2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:15754. MYL9.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. muscle myosin complex Source: ProtInc
    3. stress fiber Source: Ensembl
    4. Z disc Source: Ensembl

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31387.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 172171Myosin regulatory light polypeptide 9PRO_0000198735Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei19 – 191Phosphothreonine; by MLCK, CIT and ROCK22 Publications
    Modified residuei20 – 201Phosphoserine; by CDC42BP, CIT, MLCK, PAK1, ROCK1, ROCK2, DAPK1; DAPK2 and ZIPK/DAPK35 Publications

    Post-translational modificationi

    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP24844.
    PaxDbiP24844.
    PeptideAtlasiP24844.
    PRIDEiP24844.

    2D gel databases

    OGPiP24844.

    PTM databases

    PhosphoSiteiP24844.

    Expressioni

    Tissue specificityi

    Smooth muscle tissues and in some, but not all, nonmuscle cells.

    Gene expression databases

    BgeeiP24844.
    CleanExiHS_MYL9.
    GenevestigatoriP24844.

    Organism-specific databases

    HPAiHPA045244.

    Interactioni

    Subunit structurei

    Myosin is a hexamer of 2 heavy chains and 4 light chains.

    Protein-protein interaction databases

    BioGridi115670. 10 interactions.
    DIPiDIP-60525N.
    IntActiP24844. 4 interactions.
    MINTiMINT-1376455.
    STRINGi9606.ENSP00000279022.

    Structurei

    3D structure databases

    ProteinModelPortaliP24844.
    SMRiP24844. Positions 26-168.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 6436EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini98 – 13336EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini134 – 16936EF-hand 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 3 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5126.
    HOGENOMiHOG000233018.
    HOVERGENiHBG012180.
    InParanoidiP24844.
    KOiK12755.
    OMAiITPSKTM.
    OrthoDBiEOG7992RX.
    PhylomeDBiP24844.
    TreeFamiTF314218.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PfamiPF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 2 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 3 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P24844-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSKRAKAKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID    50
    KEDLHDMLAS LGKNPTDEYL EGMMSEAPGP INFTMFLTMF GEKLNGTDPE 100
    DVIRNAFACF DEEASGFIHE DHLRELLTTM GDRFTDEEVD EMYREAPIDK 150
    KGNFNYVEFT RILKHGAKDK DD 172
    Length:172
    Mass (Da):19,827
    Last modified:January 23, 2007 - v4
    Checksum:i4C9839E85154CDA8
    GO
    Isoform 2 (identifier: P24844-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         62-115: Missing.

    Show »
    Length:118
    Mass (Da):13,866
    Checksum:i37186D25DFC6E8CF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 101T → A in AAA59852. (PubMed:2526655)Curated
    Sequence conflicti81 – 811I → Y in AAA59852. (PubMed:2526655)Curated
    Sequence conflicti114 – 1141A → S in AAA59852. (PubMed:2526655)Curated
    Sequence conflicti125 – 1251E → K in AAA59852. (PubMed:2526655)Curated
    Sequence conflicti148 – 1481I → V in AAA59852. (PubMed:2526655)Curated
    Sequence conflicti171 – 1711D → H in AAA59852. (PubMed:2526655)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei62 – 11554Missing in isoform 2. 2 PublicationsVSP_042834Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02854 mRNA. Translation: AAA59852.1.
    AF176043 mRNA. Translation: AAQ13654.1.
    D82057 mRNA. Translation: BAB88917.1.
    AL050318 Genomic DNA. Translation: CAB75369.1.
    AL050318 Genomic DNA. Translation: CAC34440.2.
    CH471077 Genomic DNA. Translation: EAW76129.1.
    CH471077 Genomic DNA. Translation: EAW76131.1.
    CH471077 Genomic DNA. Translation: EAW76132.1.
    CH471077 Genomic DNA. Translation: EAW76133.1.
    CH471077 Genomic DNA. Translation: EAW76134.1.
    BC002648 mRNA. Translation: AAH02648.1.
    CCDSiCCDS13276.1. [P24844-1]
    CCDS13277.1. [P24844-2]
    PIRiA32031.
    RefSeqiNP_006088.2. NM_006097.4. [P24844-1]
    NP_852667.1. NM_181526.2. [P24844-2]
    UniGeneiHs.504687.

    Genome annotation databases

    EnsembliENST00000279022; ENSP00000279022; ENSG00000101335. [P24844-1]
    ENST00000346786; ENSP00000217313; ENSG00000101335. [P24844-2]
    GeneIDi10398.
    KEGGihsa:10398.
    UCSCiuc002xfl.2. human. [P24844-1]
    uc002xfm.2. human. [P24844-2]

    Polymorphism databases

    DMDMi20141521.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02854 mRNA. Translation: AAA59852.1 .
    AF176043 mRNA. Translation: AAQ13654.1 .
    D82057 mRNA. Translation: BAB88917.1 .
    AL050318 Genomic DNA. Translation: CAB75369.1 .
    AL050318 Genomic DNA. Translation: CAC34440.2 .
    CH471077 Genomic DNA. Translation: EAW76129.1 .
    CH471077 Genomic DNA. Translation: EAW76131.1 .
    CH471077 Genomic DNA. Translation: EAW76132.1 .
    CH471077 Genomic DNA. Translation: EAW76133.1 .
    CH471077 Genomic DNA. Translation: EAW76134.1 .
    BC002648 mRNA. Translation: AAH02648.1 .
    CCDSi CCDS13276.1. [P24844-1 ]
    CCDS13277.1. [P24844-2 ]
    PIRi A32031.
    RefSeqi NP_006088.2. NM_006097.4. [P24844-1 ]
    NP_852667.1. NM_181526.2. [P24844-2 ]
    UniGenei Hs.504687.

    3D structure databases

    ProteinModelPortali P24844.
    SMRi P24844. Positions 26-168.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115670. 10 interactions.
    DIPi DIP-60525N.
    IntActi P24844. 4 interactions.
    MINTi MINT-1376455.
    STRINGi 9606.ENSP00000279022.

    PTM databases

    PhosphoSitei P24844.

    Polymorphism databases

    DMDMi 20141521.

    2D gel databases

    OGPi P24844.

    Proteomic databases

    MaxQBi P24844.
    PaxDbi P24844.
    PeptideAtlasi P24844.
    PRIDEi P24844.

    Protocols and materials databases

    DNASUi 10398.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000279022 ; ENSP00000279022 ; ENSG00000101335 . [P24844-1 ]
    ENST00000346786 ; ENSP00000217313 ; ENSG00000101335 . [P24844-2 ]
    GeneIDi 10398.
    KEGGi hsa:10398.
    UCSCi uc002xfl.2. human. [P24844-1 ]
    uc002xfm.2. human. [P24844-2 ]

    Organism-specific databases

    CTDi 10398.
    GeneCardsi GC20P035169.
    HGNCi HGNC:15754. MYL9.
    HPAi HPA045244.
    MIMi 609905. gene.
    neXtProti NX_P24844.
    PharmGKBi PA31387.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5126.
    HOGENOMi HOG000233018.
    HOVERGENi HBG012180.
    InParanoidi P24844.
    KOi K12755.
    OMAi ITPSKTM.
    OrthoDBi EOG7992RX.
    PhylomeDBi P24844.
    TreeFami TF314218.

    Enzyme and pathway databases

    Reactomei REACT_19277. Sema4D induced cell migration and growth-cone collapse.
    REACT_20558. Smooth Muscle Contraction.

    Miscellaneous databases

    GeneWikii MYL9.
    GenomeRNAii 10398.
    NextBioi 39398.
    PROi P24844.
    SOURCEi Search...

    Gene expression databases

    Bgeei P24844.
    CleanExi HS_MYL9.
    Genevestigatori P24844.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    Pfami PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 2 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization and differential expression of human vascular smooth muscle myosin light chain 2 isoform in nonmuscle cells."
      Kumar C.C., Mohan S.R., Zavodny P.J., Narula S.K., Leibowitz P.J.
      Biochemistry 28:4027-4035(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Umbilical artery.
    2. "Splice variant of regulatory myosin light chain, short version."
      Pan J.Y., Li S., Silberstein D.S.
      Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    3. "Diphosphorylated MRLC is required for organization of stress fibers in interphase cells and the contractile ring in dividing cells."
      Iwasaki T., Murata-Hori M., Ishitobi S., Hosoya H.
      Cell Struct. Funct. 26:677-683(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PHOSPHORYLATION AT THR-19 AND SER-20.
    4. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Uterus.
    7. "A tripartite complex containing MRCK modulates lamellar actomyosin retrograde flow."
      Tan I., Yong J., Dong J.M., Lim L., Leung T.
      Cell 135:123-136(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-20.
    8. "Caspase-activated ROCK-1 allows erythroblast terminal maturation independently of cytokine-induced Rho signaling."
      Gabet A.S., Coulon S., Fricot A., Vandekerckhove J., Chang Y., Ribeil J.A., Lordier L., Zermati Y., Asnafi V., Belaid Z., Debili N., Vainchenker W., Varet B., Hermine O., Courtois G.
      Cell Death Differ. 18:678-689(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-20.
    9. "Chelerythrine perturbs lamellar actomyosin filaments by selective inhibition of myotonic dystrophy kinase-related Cdc42-binding kinase."
      Tan I., Lai J., Yong J., Li S.F., Leung T.
      FEBS Lett. 585:1260-1268(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT THR-19 AND SER-20.
    10. "New modularity of DAP-kinases: alternative splicing of the DRP-1 gene produces a ZIPk-like isoform."
      Shoval Y., Berissi H., Kimchi A., Pietrokovski S.
      PLoS ONE 6:E17344-E17344(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-20 BY DAPK1; DAPK2 AND ZIPK/DAPK3.

    Entry informationi

    Entry nameiMYL9_HUMAN
    AccessioniPrimary (citable) accession number: P24844
    Secondary accession number(s): E1P5T6
    , Q9BQL9, Q9BUF9, Q9H136
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 133 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    This chain binds calcium.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3