Reviewed, UniProtKB/Swiss-Prot P24823 (PPBE_MOUSE)

Names and origin

Protein names

Recommended name:
    Embryonic alkaline phosphatase
      Short name=EAP
    EC=3.1.3.1

Gene names

Name:	Akp5
Synonyms:	Eap

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion By similarity. Binds 2 zinc ions By similarity.
Subunit structure	Homodimer.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Embryo and testis.
Miscellaneous	In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).
Sequence similarities	Belongs to the alkaline phosphatase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane
Ligand	Magnesium Metal-binding Zinc
Molecular function	Hydrolase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alkaline phosphatase activity Inferred from direct assay. Source: MGI magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 502	484	Embryonic alkaline phosphatase		PRO_0000024045
	Propeptide	503 – 529	27	Removed in mature form By similarity		PRO_0000024046
Sites
	Active site	110	1	Phosphoserine intermediate
	Metal binding	60	1	Magnesium Potential
	Metal binding	60	1	Zinc 2 Potential
	Metal binding	329	1	Magnesium Potential
	Metal binding	334	1	Zinc 1 Potential
	Metal binding	338	1	Zinc 1 Potential
	Metal binding	375	1	Zinc 2 Potential
	Metal binding	376	1	Zinc 2 Potential
	Metal binding	450	1	Zinc 1 Potential
Amino acid modifications
	Lipidation	502	1	GPI-anchor amidated serine By similarity
	Glycosylation	140	1	N-linked (GlcNAc...) Potential
	Glycosylation	267	1	N-linked (GlcNAc...) Potential
	Glycosylation	277	1	N-linked (GlcNAc...) Potential
	Disulfide bond	139 ↔ 201		By similarity
	Disulfide bond	485 ↔ 492		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24823-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 2E0CD655AD4B30BA	FASTA	529	57,212
	10 20 30 40 50 60 MWGACLLLLG LSLQVCPSVI PVEEENPAFW NRKAAEALDA AKKLKPIQTS AKNLVILMGD 70 80 90 100 110 120 GMGVSTVTAT RILKGQQQGL SGPETQLAMD RFPHMALSKT YNTDKQIPDS AGTGTAFLCG 130 140 150 160 170 180 VKTNMKVIGL SAAARFNQCN TTWGNEVVSV MHRAKKAGKS VGVVTTTSVQ HASPAGTYAH 190 200 210 220 230 240 TVNRGWYSDA QMPASALQDG CKDISTQLIS NMDIDVILGG GRKFMFPKGT PDQEYPTDTK 250 260 270 280 290 300 QAGTRLDGRN LVQEWLAKHQ GARYVWNRSE LIQASLNRSV THLMGLFEPN DMKYEIHRDP 310 320 330 340 350 360 AQDPSLAEMT EVAVRMLSRN PKGFYLFVEG GRIDHGHHET VAYRALTEAV MFDSAVDKAD 370 380 390 400 410 420 IRTSEQDTMI LVTADHSHVF SFGGYTQRGA SIFGLAPFKA EDGKSFTSIL YGNGPGYKLH 430 440 450 460 470 480 NGARADVTEE ESSNPTYQQQ ACVPLSSETH SGEDVAIFAR GPQAHLVHGV QEQNYIAHVM 490 500 510 520 AFAACLEPYT DCGLASPAGQ SSAVSPGYMS TLLCLLAGKM LMLMAAAEP

References

[1]	"Genomic structure and comparison of mouse tissue-specific alkaline phosphatase genes." Manes T., Glade K., Ziomek C.A., Millan J.L. Genomics 8:541-554(1990) [PubMed: 2286375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M61704 Genomic DNA. Translation: AAA37531.1.
IPI	IPI00111122.
UniGene	Mm.195224
3D structure databases
HSSP	HSSP built from PDB template 1EW2 based on UniProtKB P05187.
SMR	P24823. Positions 19-497.
ModBase	Search...
PTM databases
PhosphoSite	P24823.
Genome annotation databases
Ensembl	ENSMUSG00000026246. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:108009. Akp5.
Phylogenomic databases
HOGENOM	P24823.
HOVERGEN	P24823.
Enzyme and pathway databases
BRENDA	3.1.3.1. 244.
Gene expression databases
ArrayExpress	P24823.
Bgee	P24823.
CleanEx	MM_AKP5.
GermOnline	ENSMUSG00000026246. Mus musculus.
Family and domain databases
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR001952. Alkaline_phosphatase. IPR018299. Alkaline_phosphatase_AS. [Graphical view]
Gene3D	G3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
Pfam	PF00245. Alk_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00113. ALKPHPHTASE.
SMART	SM00098. alkPPc. 1 hit. [Graphical view]
PROSITE	PS00123. ALKALINE_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

PPBE_MOUSE

Accession

Primary (citable) accession number: P24823

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)