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P24823

- PPBN_MOUSE

UniProt

P24823 - PPBN_MOUSE

Protein

Alkaline phosphatase, placental-like

Gene

Alppl2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi60 – 601MagnesiumBy similarity
    Metal bindingi60 – 601Zinc 1By similarity
    Active sitei110 – 1101Phosphoserine intermediate
    Metal bindingi110 – 1101Zinc 1By similarity
    Metal bindingi173 – 1731MagnesiumBy similarity
    Metal bindingi329 – 3291MagnesiumBy similarity
    Metal bindingi334 – 3341Zinc 2By similarity
    Metal bindingi338 – 3381Zinc 2; via tele nitrogenBy similarity
    Metal bindingi375 – 3751Zinc 1By similarity
    Metal bindingi376 – 3761Zinc 1; via tele nitrogenBy similarity
    Metal bindingi450 – 4501Zinc 2; via tele nitrogenBy similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: MGI
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase, placental-like (EC:3.1.3.1)
    Short name:
    EAP
    Short name:
    Embryonic alkaline phosphatase
    Alternative name(s):
    Alkaline phosphatase 5
    Embryonic-type alkaline phosphatase
    Gene namesi
    Name:Alppl2
    Synonyms:Akp5, Eap
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:108009. Alppl2.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. myofibril Source: MGI
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 502484Alkaline phosphatase, placental-likePRO_0000024045Add
    BLAST
    Propeptidei503 – 52927Removed in mature formBy similarityPRO_0000024046Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi139 ↔ 201By similarity
    Glycosylationi140 – 1401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi485 ↔ 492By similarity
    Lipidationi502 – 5021GPI-anchor amidated serineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiP24823.

    PTM databases

    PhosphoSiteiP24823.

    Expressioni

    Tissue specificityi

    Embryo and testis.

    Gene expression databases

    ArrayExpressiP24823.
    BgeeiP24823.
    CleanExiMM_AKP5.
    GenevestigatoriP24823.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000027455.

    Structurei

    3D structure databases

    ProteinModelPortaliP24823.
    SMRiP24823. Positions 19-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1785.
    GeneTreeiENSGT00390000008704.
    HOGENOMiHOG000099118.
    HOVERGENiHBG007345.
    InParanoidiQ3ULC9.
    KOiK01077.
    OMAiKTNMKVI.
    OrthoDBiEOG7SN8CH.
    TreeFamiTF323513.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24823-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWGACLLLLG LSLQVCPSVI PVEEENPAFW NRKAAEALDA AKKLKPIQTS    50
    AKNLVILMGD GMGVSTVTAT RILKGQQQGH LGPETQLAMD RFPHMALSKT 100
    YNTDKQIPDS AGTGTAFLCG VKTNMKVIGL SAAARFNQCN TTWGNEVVSV 150
    MHRAKKAGKS VGVVTTTSVQ HASPAGTYAH TVNRGWYSDA QMPASALQDG 200
    CKDISTQLIS NMDIDVILGG GRKFMFPKGT PDQEYPTDTK QAGTRLDGRN 250
    LVQEWLAKHQ GARYVWNRSE LIQASLNRSV THLMGLFEPN DMKYEIHRDP 300
    AQDPSLAEMT EVAVRMLSRN PKGFYLFVEG GRIDHGHHET VAYRALTEAV 350
    MFDSAVDKAD KLTSEQDTMI LVTADHSHVF SFGGYTQRGA SIFGLAPFKA 400
    EDGKSFTSIL YGNGPGYKLH NGARADVTEE ESSNPTYQQQ AAVPLSSETH 450
    SGEDVAIFAR GPQAHLVHGV QEQNYIAHVM AFAACLEPYT DCGLASPAGQ 500
    SSAVSPGYMS TLLCLLAGKM LMLMAAAEP 529
    Length:529
    Mass (Da):57,202
    Last modified:July 27, 2011 - v2
    Checksum:i540A95D21D90BDEC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti80 – 812HL → LS in AAA37531. (PubMed:2286375)Curated
    Sequence conflicti361 – 3622KL → IR in AAA37531. (PubMed:2286375)Curated
    Sequence conflicti442 – 4421A → C in AAA37531. (PubMed:2286375)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M61704 Genomic DNA. Translation: AAA37531.1.
    AK145571 mRNA. Translation: BAE26520.1.
    CH466520 Genomic DNA. Translation: EDL40194.1.
    CCDSiCCDS15126.1.
    RefSeqiNP_031459.3. NM_007433.3.
    XP_006529145.1. XM_006529082.1.
    UniGeneiMm.195224.

    Genome annotation databases

    EnsembliENSMUST00000027455; ENSMUSP00000027455; ENSMUSG00000026246.
    GeneIDi11650.
    KEGGimmu:11650.
    UCSCiuc007bvy.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M61704 Genomic DNA. Translation: AAA37531.1 .
    AK145571 mRNA. Translation: BAE26520.1 .
    CH466520 Genomic DNA. Translation: EDL40194.1 .
    CCDSi CCDS15126.1.
    RefSeqi NP_031459.3. NM_007433.3.
    XP_006529145.1. XM_006529082.1.
    UniGenei Mm.195224.

    3D structure databases

    ProteinModelPortali P24823.
    SMRi P24823. Positions 19-500.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000027455.

    PTM databases

    PhosphoSitei P24823.

    Proteomic databases

    PRIDEi P24823.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027455 ; ENSMUSP00000027455 ; ENSMUSG00000026246 .
    GeneIDi 11650.
    KEGGi mmu:11650.
    UCSCi uc007bvy.1. mouse.

    Organism-specific databases

    CTDi 251.
    MGIi MGI:108009. Alppl2.

    Phylogenomic databases

    eggNOGi COG1785.
    GeneTreei ENSGT00390000008704.
    HOGENOMi HOG000099118.
    HOVERGENi HBG007345.
    InParanoidi Q3ULC9.
    KOi K01077.
    OMAi KTNMKVI.
    OrthoDBi EOG7SN8CH.
    TreeFami TF323513.

    Miscellaneous databases

    NextBioi 279253.
    PROi P24823.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P24823.
    Bgeei P24823.
    CleanExi MM_AKP5.
    Genevestigatori P24823.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic structure and comparison of mouse tissue-specific alkaline phosphatase genes."
      Manes T., Glade K., Ziomek C.A., Millan J.L.
      Genomics 8:541-554(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiPPBN_MOUSE
    AccessioniPrimary (citable) accession number: P24823
    Secondary accession number(s): Q3ULC9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3