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P24823

- PPBN_MOUSE

UniProt

P24823 - PPBN_MOUSE

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Protein

Alkaline phosphatase, placental-like

Gene

Alppl2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg(2+) ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn(2+) ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601MagnesiumBy similarity
Metal bindingi60 – 601Zinc 1By similarity
Active sitei110 – 1101Phosphoserine intermediate
Metal bindingi110 – 1101Zinc 1By similarity
Metal bindingi173 – 1731MagnesiumBy similarity
Metal bindingi329 – 3291MagnesiumBy similarity
Metal bindingi334 – 3341Zinc 2By similarity
Metal bindingi338 – 3381Zinc 2; via tele nitrogenBy similarity
Metal bindingi375 – 3751Zinc 1By similarity
Metal bindingi376 – 3761Zinc 1; via tele nitrogenBy similarity
Metal bindingi450 – 4501Zinc 2; via tele nitrogenBy similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: MGI
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, placental-like (EC:3.1.3.1)
Short name:
EAP
Short name:
Embryonic alkaline phosphatase
Alternative name(s):
Alkaline phosphatase 5
Embryonic-type alkaline phosphatase
Gene namesi
Name:Alppl2
Synonyms:Akp5, Eap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:108009. Alppl2.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. myofibril Source: MGI
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 502484Alkaline phosphatase, placental-likePRO_0000024045Add
BLAST
Propeptidei503 – 52927Removed in mature formBy similarityPRO_0000024046Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi139 ↔ 201By similarity
Glycosylationi140 – 1401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi485 ↔ 492By similarity
Lipidationi502 – 5021GPI-anchor amidated serineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PRIDEiP24823.

PTM databases

PhosphoSiteiP24823.

Expressioni

Tissue specificityi

Embryo and testis.

Gene expression databases

BgeeiP24823.
CleanExiMM_AKP5.
ExpressionAtlasiP24823. baseline and differential.
GenevestigatoriP24823.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027455.

Structurei

3D structure databases

ProteinModelPortaliP24823.
SMRiP24823. Positions 19-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1785.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP24823.
KOiK01077.
OMAiKTNMKVI.
OrthoDBiEOG7SN8CH.
TreeFamiTF323513.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24823-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWGACLLLLG LSLQVCPSVI PVEEENPAFW NRKAAEALDA AKKLKPIQTS
60 70 80 90 100
AKNLVILMGD GMGVSTVTAT RILKGQQQGH LGPETQLAMD RFPHMALSKT
110 120 130 140 150
YNTDKQIPDS AGTGTAFLCG VKTNMKVIGL SAAARFNQCN TTWGNEVVSV
160 170 180 190 200
MHRAKKAGKS VGVVTTTSVQ HASPAGTYAH TVNRGWYSDA QMPASALQDG
210 220 230 240 250
CKDISTQLIS NMDIDVILGG GRKFMFPKGT PDQEYPTDTK QAGTRLDGRN
260 270 280 290 300
LVQEWLAKHQ GARYVWNRSE LIQASLNRSV THLMGLFEPN DMKYEIHRDP
310 320 330 340 350
AQDPSLAEMT EVAVRMLSRN PKGFYLFVEG GRIDHGHHET VAYRALTEAV
360 370 380 390 400
MFDSAVDKAD KLTSEQDTMI LVTADHSHVF SFGGYTQRGA SIFGLAPFKA
410 420 430 440 450
EDGKSFTSIL YGNGPGYKLH NGARADVTEE ESSNPTYQQQ AAVPLSSETH
460 470 480 490 500
SGEDVAIFAR GPQAHLVHGV QEQNYIAHVM AFAACLEPYT DCGLASPAGQ
510 520
SSAVSPGYMS TLLCLLAGKM LMLMAAAEP
Length:529
Mass (Da):57,202
Last modified:July 27, 2011 - v2
Checksum:i540A95D21D90BDEC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti80 – 812HL → LS in AAA37531. (PubMed:2286375)Curated
Sequence conflicti361 – 3622KL → IR in AAA37531. (PubMed:2286375)Curated
Sequence conflicti442 – 4421A → C in AAA37531. (PubMed:2286375)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61704 Genomic DNA. Translation: AAA37531.1.
AK145571 mRNA. Translation: BAE26520.1.
CH466520 Genomic DNA. Translation: EDL40194.1.
CCDSiCCDS15126.1.
RefSeqiNP_031459.3. NM_007433.3.
XP_006529145.1. XM_006529082.1.
UniGeneiMm.195224.

Genome annotation databases

EnsembliENSMUST00000027455; ENSMUSP00000027455; ENSMUSG00000026246.
ENSMUST00000188310; ENSMUSP00000139887; ENSMUSG00000026246.
GeneIDi11650.
KEGGimmu:11650.
UCSCiuc007bvy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61704 Genomic DNA. Translation: AAA37531.1 .
AK145571 mRNA. Translation: BAE26520.1 .
CH466520 Genomic DNA. Translation: EDL40194.1 .
CCDSi CCDS15126.1.
RefSeqi NP_031459.3. NM_007433.3.
XP_006529145.1. XM_006529082.1.
UniGenei Mm.195224.

3D structure databases

ProteinModelPortali P24823.
SMRi P24823. Positions 19-500.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000027455.

Chemistry

ChEMBLi CHEMBL3112374.

PTM databases

PhosphoSitei P24823.

Proteomic databases

PRIDEi P24823.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027455 ; ENSMUSP00000027455 ; ENSMUSG00000026246 .
ENSMUST00000188310 ; ENSMUSP00000139887 ; ENSMUSG00000026246 .
GeneIDi 11650.
KEGGi mmu:11650.
UCSCi uc007bvy.1. mouse.

Organism-specific databases

CTDi 251.
MGIi MGI:108009. Alppl2.

Phylogenomic databases

eggNOGi COG1785.
GeneTreei ENSGT00390000008704.
HOGENOMi HOG000099118.
HOVERGENi HBG007345.
InParanoidi P24823.
KOi K01077.
OMAi KTNMKVI.
OrthoDBi EOG7SN8CH.
TreeFami TF323513.

Miscellaneous databases

NextBioi 279253.
PROi P24823.
SOURCEi Search...

Gene expression databases

Bgeei P24823.
CleanExi MM_AKP5.
ExpressionAtlasi P24823. baseline and differential.
Genevestigatori P24823.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 1 hit.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic structure and comparison of mouse tissue-specific alkaline phosphatase genes."
    Manes T., Glade K., Ziomek C.A., Millan J.L.
    Genomics 8:541-554(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiPPBN_MOUSE
AccessioniPrimary (citable) accession number: P24823
Secondary accession number(s): Q3ULC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3