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Protein

Alkaline phosphatase, placental-like

Gene

Alppl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60MagnesiumBy similarity1
Metal bindingi60Zinc 1By similarity1
Active sitei110Phosphoserine intermediate1
Metal bindingi110Zinc 1By similarity1
Metal bindingi173MagnesiumBy similarity1
Metal bindingi329MagnesiumBy similarity1
Metal bindingi334Zinc 2By similarity1
Metal bindingi338Zinc 2; via tele nitrogenBy similarity1
Metal bindingi375Zinc 1By similarity1
Metal bindingi376Zinc 1; via tele nitrogenBy similarity1
Metal bindingi450Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • alkaline phosphatase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1483166. Synthesis of PA.
R-MMU-6811438. Intra-Golgi traffic.
R-MMU-8935690. Miscellaneous digestion events.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, placental-like (EC:3.1.3.1)
Alternative name(s):
Alkaline phosphatase 5
Embryonic alkaline phosphatase
Short name:
EAP
Embryonic-type alkaline phosphatase
Gene namesi
Name:Alppl2
Synonyms:Akp5, Eap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:108009. Alppl2.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • myofibril Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3112374.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000002404519 – 502Alkaline phosphatase, placental-likeAdd BLAST484
PropeptideiPRO_0000024046503 – 529Removed in mature formBy similarityAdd BLAST27

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi139 ↔ 201By similarity
Glycosylationi140N-linked (GlcNAc...)Sequence analysis1
Glycosylationi267N-linked (GlcNAc...)Sequence analysis1
Glycosylationi277N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi485 ↔ 492By similarity
Lipidationi502GPI-anchor amidated serineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP24823.
PaxDbiP24823.
PeptideAtlasiP24823.
PRIDEiP24823.

PTM databases

PhosphoSitePlusiP24823.

Expressioni

Tissue specificityi

Embryo and testis.

Gene expression databases

BgeeiENSMUSG00000026246.
CleanExiMM_AKP5.
GenevisibleiP24823. MM.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027455.

Chemistry databases

BindingDBiP24823.

Structurei

3D structure databases

ProteinModelPortaliP24823.
SMRiP24823.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP24823.
KOiK01077.
OMAiHSPERNW.
OrthoDBiEOG091G067H.
TreeFamiTF323513.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24823-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGACLLLLG LSLQVCPSVI PVEEENPAFW NRKAAEALDA AKKLKPIQTS
60 70 80 90 100
AKNLVILMGD GMGVSTVTAT RILKGQQQGH LGPETQLAMD RFPHMALSKT
110 120 130 140 150
YNTDKQIPDS AGTGTAFLCG VKTNMKVIGL SAAARFNQCN TTWGNEVVSV
160 170 180 190 200
MHRAKKAGKS VGVVTTTSVQ HASPAGTYAH TVNRGWYSDA QMPASALQDG
210 220 230 240 250
CKDISTQLIS NMDIDVILGG GRKFMFPKGT PDQEYPTDTK QAGTRLDGRN
260 270 280 290 300
LVQEWLAKHQ GARYVWNRSE LIQASLNRSV THLMGLFEPN DMKYEIHRDP
310 320 330 340 350
AQDPSLAEMT EVAVRMLSRN PKGFYLFVEG GRIDHGHHET VAYRALTEAV
360 370 380 390 400
MFDSAVDKAD KLTSEQDTMI LVTADHSHVF SFGGYTQRGA SIFGLAPFKA
410 420 430 440 450
EDGKSFTSIL YGNGPGYKLH NGARADVTEE ESSNPTYQQQ AAVPLSSETH
460 470 480 490 500
SGEDVAIFAR GPQAHLVHGV QEQNYIAHVM AFAACLEPYT DCGLASPAGQ
510 520
SSAVSPGYMS TLLCLLAGKM LMLMAAAEP
Length:529
Mass (Da):57,202
Last modified:July 27, 2011 - v2
Checksum:i540A95D21D90BDEC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80 – 81HL → LS in AAA37531 (PubMed:2286375).Curated2
Sequence conflicti361 – 362KL → IR in AAA37531 (PubMed:2286375).Curated2
Sequence conflicti442A → C in AAA37531 (PubMed:2286375).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61704 Genomic DNA. Translation: AAA37531.1.
AK145571 mRNA. Translation: BAE26520.1.
CH466520 Genomic DNA. Translation: EDL40194.1.
CCDSiCCDS15126.1.
RefSeqiNP_031459.3. NM_007433.3.
XP_006529145.1. XM_006529082.3.
XP_011246206.1. XM_011247904.2.
UniGeneiMm.195224.

Genome annotation databases

EnsembliENSMUST00000027455; ENSMUSP00000027455; ENSMUSG00000026246.
ENSMUST00000188310; ENSMUSP00000139887; ENSMUSG00000026246.
GeneIDi11650.
KEGGimmu:11650.
UCSCiuc007bvy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61704 Genomic DNA. Translation: AAA37531.1.
AK145571 mRNA. Translation: BAE26520.1.
CH466520 Genomic DNA. Translation: EDL40194.1.
CCDSiCCDS15126.1.
RefSeqiNP_031459.3. NM_007433.3.
XP_006529145.1. XM_006529082.3.
XP_011246206.1. XM_011247904.2.
UniGeneiMm.195224.

3D structure databases

ProteinModelPortaliP24823.
SMRiP24823.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027455.

Chemistry databases

BindingDBiP24823.
ChEMBLiCHEMBL3112374.

PTM databases

PhosphoSitePlusiP24823.

Proteomic databases

MaxQBiP24823.
PaxDbiP24823.
PeptideAtlasiP24823.
PRIDEiP24823.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027455; ENSMUSP00000027455; ENSMUSG00000026246.
ENSMUST00000188310; ENSMUSP00000139887; ENSMUSG00000026246.
GeneIDi11650.
KEGGimmu:11650.
UCSCiuc007bvy.1. mouse.

Organism-specific databases

CTDi251.
MGIiMGI:108009. Alppl2.

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP24823.
KOiK01077.
OMAiHSPERNW.
OrthoDBiEOG091G067H.
TreeFamiTF323513.

Enzyme and pathway databases

ReactomeiR-MMU-1483166. Synthesis of PA.
R-MMU-6811438. Intra-Golgi traffic.
R-MMU-8935690. Miscellaneous digestion events.

Miscellaneous databases

PROiP24823.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026246.
CleanExiMM_AKP5.
GenevisibleiP24823. MM.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPBN_MOUSE
AccessioniPrimary (citable) accession number: P24823
Secondary accession number(s): Q3ULC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.