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P24823 (PPBN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alkaline phosphatase, placental-like

Short name=EAP
Short name=Embryonic alkaline phosphatase
EC=3.1.3.1
Alternative name(s):
Alkaline phosphatase 5
Embryonic-type alkaline phosphatase
Gene names
Name:Alppl2
Synonyms:Akp5, Eap
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length529 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Subunit structure

Homodimer.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor.

Tissue specificity

Embryo and testis.

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Sequence similarities

Belongs to the alkaline phosphatase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 502484Alkaline phosphatase, placental-like
PRO_0000024045
Propeptide503 – 52927Removed in mature form By similarity
PRO_0000024046

Sites

Active site1101Phosphoserine intermediate
Metal binding601Magnesium By similarity
Metal binding601Zinc 1 By similarity
Metal binding1101Zinc 1 By similarity
Metal binding1731Magnesium By similarity
Metal binding3291Magnesium By similarity
Metal binding3341Zinc 2 By similarity
Metal binding3381Zinc 2; via tele nitrogen By similarity
Metal binding3751Zinc 1 By similarity
Metal binding3761Zinc 1; via tele nitrogen By similarity
Metal binding4501Zinc 2; via tele nitrogen By similarity

Amino acid modifications

Lipidation5021GPI-anchor amidated serine By similarity
Glycosylation1401N-linked (GlcNAc...) Potential
Glycosylation2671N-linked (GlcNAc...) Potential
Glycosylation2771N-linked (GlcNAc...) Potential
Disulfide bond139 ↔ 201 By similarity
Disulfide bond485 ↔ 492 By similarity

Experimental info

Sequence conflict80 – 812HL → LS in AAA37531. Ref.1
Sequence conflict361 – 3622KL → IR in AAA37531. Ref.1
Sequence conflict4421A → C in AAA37531. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24823 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 540A95D21D90BDEC

FASTA52957,202
        10         20         30         40         50         60 
MWGACLLLLG LSLQVCPSVI PVEEENPAFW NRKAAEALDA AKKLKPIQTS AKNLVILMGD 

        70         80         90        100        110        120 
GMGVSTVTAT RILKGQQQGH LGPETQLAMD RFPHMALSKT YNTDKQIPDS AGTGTAFLCG 

       130        140        150        160        170        180 
VKTNMKVIGL SAAARFNQCN TTWGNEVVSV MHRAKKAGKS VGVVTTTSVQ HASPAGTYAH 

       190        200        210        220        230        240 
TVNRGWYSDA QMPASALQDG CKDISTQLIS NMDIDVILGG GRKFMFPKGT PDQEYPTDTK 

       250        260        270        280        290        300 
QAGTRLDGRN LVQEWLAKHQ GARYVWNRSE LIQASLNRSV THLMGLFEPN DMKYEIHRDP 

       310        320        330        340        350        360 
AQDPSLAEMT EVAVRMLSRN PKGFYLFVEG GRIDHGHHET VAYRALTEAV MFDSAVDKAD 

       370        380        390        400        410        420 
KLTSEQDTMI LVTADHSHVF SFGGYTQRGA SIFGLAPFKA EDGKSFTSIL YGNGPGYKLH 

       430        440        450        460        470        480 
NGARADVTEE ESSNPTYQQQ AAVPLSSETH SGEDVAIFAR GPQAHLVHGV QEQNYIAHVM 

       490        500        510        520 
AFAACLEPYT DCGLASPAGQ SSAVSPGYMS TLLCLLAGKM LMLMAAAEP 

« Hide

References

« Hide 'large scale' references
[1]"Genomic structure and comparison of mouse tissue-specific alkaline phosphatase genes."
Manes T., Glade K., Ziomek C.A., Millan J.L.
Genomics 8:541-554(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M61704 Genomic DNA. Translation: AAA37531.1.
AK145571 mRNA. Translation: BAE26520.1.
CH466520 Genomic DNA. Translation: EDL40194.1.
RefSeqNP_031459.3. NM_007433.3.
XP_006529145.1. XM_006529082.1.
UniGeneMm.195224.

3D structure databases

ProteinModelPortalP24823.
SMRP24823. Positions 19-500.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000027455.

PTM databases

PhosphoSiteP24823.

Proteomic databases

PRIDEP24823.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027455; ENSMUSP00000027455; ENSMUSG00000026246.
GeneID11650.
KEGGmmu:11650.
UCSCuc007bvy.1. mouse.

Organism-specific databases

CTD251.
MGIMGI:108009. Alppl2.

Phylogenomic databases

eggNOGCOG1785.
GeneTreeENSGT00390000008704.
HOGENOMHOG000099118.
HOVERGENHBG007345.
InParanoidQ3ULC9.
KOK01077.
OMATTWGNEV.
OrthoDBEOG7SN8CH.
TreeFamTF323513.

Gene expression databases

ArrayExpressP24823.
BgeeP24823.
CleanExMM_AKP5.
GenevestigatorP24823.

Family and domain databases

Gene3D3.40.720.10. 1 hit.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMSSF53649. SSF53649. 1 hit.
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279253.
PROP24823.
SOURCESearch...

Entry information

Entry namePPBN_MOUSE
AccessionPrimary (citable) accession number: P24823
Secondary accession number(s): Q3ULC9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 27, 2011
Last modified: March 19, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot