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P24822

- PPBI_MOUSE

UniProt

P24822 - PPBI_MOUSE

Protein

Intestinal-type alkaline phosphatase

Gene

Iap

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi61 – 611MagnesiumBy similarity
    Metal bindingi61 – 611Zinc 1By similarity
    Active sitei111 – 1111Phosphoserine intermediate
    Metal bindingi111 – 1111Zinc 1By similarity
    Metal bindingi174 – 1741MagnesiumBy similarity
    Metal bindingi330 – 3301MagnesiumBy similarity
    Metal bindingi335 – 3351Zinc 2By similarity
    Metal bindingi339 – 3391Zinc 2; via tele nitrogenBy similarity
    Metal bindingi376 – 3761Zinc 1By similarity
    Metal bindingi377 – 3771Zinc 1; via tele nitrogenBy similarity
    Metal bindingi450 – 4501Zinc 2; via tele nitrogenBy similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. dephosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Intestinal-type alkaline phosphatase (EC:3.1.3.1)
    Short name:
    IAP
    Short name:
    Intestinal alkaline phosphatase
    Alternative name(s):
    Alkaline phosphatase 3
    Gene namesi
    Name:Iap
    Synonyms:Akp-3, Akp3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:87984. Akp3.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 528509Intestinal-type alkaline phosphatasePRO_0000024039Add
    BLAST
    Propeptidei529 – 55931Removed in mature formSequence AnalysisPRO_0000024040Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi140 ↔ 202By similarity
    Glycosylationi141 – 1411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi241 – 2411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi426 – 4261N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi485 ↔ 492By similarity
    Lipidationi528 – 5281GPI-anchor amidated asparagineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP24822.
    PaxDbiP24822.
    PRIDEiP24822.

    PTM databases

    PhosphoSiteiP24822.

    Expressioni

    Tissue specificityi

    Intestine and thymus.

    Gene expression databases

    GenevestigatoriP24822.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000037497.

    Structurei

    3D structure databases

    ProteinModelPortaliP24822.
    SMRiP24822. Positions 20-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi503 – 5075Poly-Thr
    Compositional biasi510 – 52415Poly-ThrAdd
    BLAST

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1785.
    HOGENOMiHOG000099118.
    HOVERGENiHBG007345.
    InParanoidiP24822.
    PhylomeDBiP24822.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24822-1 [UniParc]FASTAAdd to Basket

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    MQGPWVLLLL GLRLQLSLSV IPVEEENPAF WNKKAAEALD AAKKLQPIQT    50
    SAKNLIIFLG DGMGVPTVTA TRILKGQLEG HLGPETPLAM DRFPYMALSK 100
    TYSVDRQVPD SASTATAYLC GVKTNYKTIG LSAAARFDQC NTTFGNEVFS 150
    VMYRAKKAGK SVGVVTTTRV QHASPSGTYV HTVNRNWYGD ADMPASALRE 200
    GCKDIATQLI SNMDINVILG GGRKYMFPAG TPDPEYPNDA NETGTRLDGR 250
    NLVQEWLSKH QGSQYVWNRE QLIQKAQDPS VTYLMGLFEP VDTKFDIQRD 300
    PLMDPSLKDM TETAVKVLSR NPKGFYLFVE GGRIDRGHHL GTAYLALTEA 350
    VMFDLAIERA SQLTSERDTL TIVTADHSHV FSFGGYTLRG TSIFGLAPLN 400
    ALDGKPYTSI LYGNGPGYVG TGERPNVTAA ESSGSSYRRQ AAVPVKSETH 450
    GGEDVAIFAR GPQAHLVHGV QEQNYIAHVM ASAGCLEPYT DCGLAPPADE 500
    SQTTTTTRQT TITTTTTTTT TTTTPVHNSA RSLGPATAPL ALALLAGMLM 550
    LLLGAPAES 559
    Length:559
    Mass (Da):60,285
    Last modified:March 1, 1992 - v1
    Checksum:i019EBB4363950878
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M61705 Genomic DNA. Translation: AAA37873.1.
    CCDSiCCDS15127.1.
    PIRiB36307.
    UniGeneiMm.377076.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M61705 Genomic DNA. Translation: AAA37873.1 .
    CCDSi CCDS15127.1.
    PIRi B36307.
    UniGenei Mm.377076.

    3D structure databases

    ProteinModelPortali P24822.
    SMRi P24822. Positions 20-500.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000037497.

    Chemistry

    ChEMBLi CHEMBL3151.

    PTM databases

    PhosphoSitei P24822.

    Proteomic databases

    MaxQBi P24822.
    PaxDbi P24822.
    PRIDEi P24822.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:87984. Akp3.

    Phylogenomic databases

    eggNOGi COG1785.
    HOGENOMi HOG000099118.
    HOVERGENi HBG007345.
    InParanoidi P24822.
    PhylomeDBi P24822.

    Miscellaneous databases

    SOURCEi Search...

    Gene expression databases

    Genevestigatori P24822.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic structure and comparison of mouse tissue-specific alkaline phosphatase genes."
      Manes T., Glade K., Ziomek C.A., Millan J.L.
      Genomics 8:541-554(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiPPBI_MOUSE
    AccessioniPrimary (citable) accession number: P24822
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3