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P24822 (PPBI_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Intestinal-type alkaline phosphatase
Short name=IAP
Short name=Intestinal alkaline phosphatase
EC=3.1.3.1
Alternative name(s):
Alkaline phosphatase 3
Gene names
Name:Iap
Synonyms:Akp-3, Akp3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length559 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Subunit structure

Homodimer.

Subcellular location

Cell membrane; Lipid-anchor › GPI-anchor Potential.

Tissue specificity

Intestine and thymus.

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Sequence similarities

Belongs to the alkaline phosphatase family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainSignal
   LigandMagnesium
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMDisulfide bond
GPI-anchor
Glycoprotein
Lipoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdephosphorylation

Inferred from electronic annotation. Source: GOC

   Cellular_componentanchored to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionalkaline phosphatase activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 528509Intestinal-type alkaline phosphatase
PRO_0000024039
Propeptide529 – 55931Removed in mature form Potential
PRO_0000024040

Regions

Compositional bias503 – 5075Poly-Thr
Compositional bias510 – 52415Poly-Thr

Sites

Active site1111Phosphoserine intermediate
Metal binding611Magnesium Potential
Metal binding611Zinc 2 Potential
Metal binding3301Magnesium Potential
Metal binding3351Zinc 1 Potential
Metal binding3391Zinc 1 Potential
Metal binding3761Zinc 2 Potential
Metal binding3771Zinc 2 Potential
Metal binding4501Zinc 1 Potential

Amino acid modifications

Lipidation5281GPI-anchor amidated asparagine Potential
Glycosylation1411N-linked (GlcNAc...) Potential
Glycosylation2411N-linked (GlcNAc...) Potential
Glycosylation4261N-linked (GlcNAc...) Potential
Disulfide bond140 ↔ 202 By similarity
Disulfide bond485 ↔ 492 By similarity

Sequences

Sequence LengthMass (Da)Tools
P24822 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 019EBB4363950878

FASTA55960,285
        10         20         30         40         50         60 
MQGPWVLLLL GLRLQLSLSV IPVEEENPAF WNKKAAEALD AAKKLQPIQT SAKNLIIFLG 

        70         80         90        100        110        120 
DGMGVPTVTA TRILKGQLEG HLGPETPLAM DRFPYMALSK TYSVDRQVPD SASTATAYLC 

       130        140        150        160        170        180 
GVKTNYKTIG LSAAARFDQC NTTFGNEVFS VMYRAKKAGK SVGVVTTTRV QHASPSGTYV 

       190        200        210        220        230        240 
HTVNRNWYGD ADMPASALRE GCKDIATQLI SNMDINVILG GGRKYMFPAG TPDPEYPNDA 

       250        260        270        280        290        300 
NETGTRLDGR NLVQEWLSKH QGSQYVWNRE QLIQKAQDPS VTYLMGLFEP VDTKFDIQRD 

       310        320        330        340        350        360 
PLMDPSLKDM TETAVKVLSR NPKGFYLFVE GGRIDRGHHL GTAYLALTEA VMFDLAIERA 

       370        380        390        400        410        420 
SQLTSERDTL TIVTADHSHV FSFGGYTLRG TSIFGLAPLN ALDGKPYTSI LYGNGPGYVG 

       430        440        450        460        470        480 
TGERPNVTAA ESSGSSYRRQ AAVPVKSETH GGEDVAIFAR GPQAHLVHGV QEQNYIAHVM 

       490        500        510        520        530        540 
ASAGCLEPYT DCGLAPPADE SQTTTTTRQT TITTTTTTTT TTTTPVHNSA RSLGPATAPL 

       550 
ALALLAGMLM LLLGAPAES

« Hide

References

[1]"Genomic structure and comparison of mouse tissue-specific alkaline phosphatase genes."
Manes T., Glade K., Ziomek C.A., Millan J.L.
Genomics 8:541-554(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M61705 Genomic DNA. Translation: AAA37873.1.
IPIIPI00111121.
PIRB36307.
UniGeneMm.377076.

3D structure databases

ProteinModelPortalP24822.
SMRP24822. Positions 20-498.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000037497.

PTM databases

PhosphoSiteP24822.

Proteomic databases

PaxDbP24822.
PRIDEP24822.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:87984. Akp3.

Phylogenomic databases

eggNOGCOG1785.
HOGENOMHOG000099118.
HOVERGENHBG007345.
InParanoidP24822.
OrthoDBEOG4GB765.

Gene expression databases

GenevestigatorP24822.
GermOnlineENSMUSG00000036500. Mus musculus.

Family and domain databases

Gene3D3.40.720.10. 1 hit.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMSSF53649. Alkaline_phosphatase_core. 1 hit.
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChEMBLCHEMBL3151.
SOURCESearch...

Entry information

Entry namePPBI_MOUSE
AccessionPrimary (citable) accession number: P24822
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: April 3, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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