Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Intestinal-type alkaline phosphatase

Gene

Iap

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611MagnesiumBy similarity
Metal bindingi61 – 611Zinc 1By similarity
Active sitei111 – 1111Phosphoserine intermediate
Metal bindingi111 – 1111Zinc 1By similarity
Metal bindingi174 – 1741MagnesiumBy similarity
Metal bindingi330 – 3301MagnesiumBy similarity
Metal bindingi335 – 3351Zinc 2By similarity
Metal bindingi339 – 3391Zinc 2; via tele nitrogenBy similarity
Metal bindingi376 – 3761Zinc 1By similarity
Metal bindingi377 – 3771Zinc 1; via tele nitrogenBy similarity
Metal bindingi450 – 4501Zinc 2; via tele nitrogenBy similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Intestinal-type alkaline phosphatase (EC:3.1.3.1)
Short name:
IAP
Short name:
Intestinal alkaline phosphatase
Alternative name(s):
Alkaline phosphatase 3
Gene namesi
Name:Iap
Synonyms:Akp-3, Akp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:87984. Akp3.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 528509Intestinal-type alkaline phosphatasePRO_0000024039Add
BLAST
Propeptidei529 – 55931Removed in mature formSequence AnalysisPRO_0000024040Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi140 ↔ 202By similarity
Glycosylationi141 – 1411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi241 – 2411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi426 – 4261N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi485 ↔ 492By similarity
Lipidationi528 – 5281GPI-anchor amidated asparagineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiP24822.
PaxDbiP24822.
PRIDEiP24822.

PTM databases

PhosphoSiteiP24822.

Expressioni

Tissue specificityi

Intestine and thymus.

Gene expression databases

GenevestigatoriP24822.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037497.

Structurei

3D structure databases

ProteinModelPortaliP24822.
SMRiP24822. Positions 20-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi503 – 5075Poly-Thr
Compositional biasi510 – 52415Poly-ThrAdd
BLAST

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1785.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP24822.
PhylomeDBiP24822.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGPWVLLLL GLRLQLSLSV IPVEEENPAF WNKKAAEALD AAKKLQPIQT
60 70 80 90 100
SAKNLIIFLG DGMGVPTVTA TRILKGQLEG HLGPETPLAM DRFPYMALSK
110 120 130 140 150
TYSVDRQVPD SASTATAYLC GVKTNYKTIG LSAAARFDQC NTTFGNEVFS
160 170 180 190 200
VMYRAKKAGK SVGVVTTTRV QHASPSGTYV HTVNRNWYGD ADMPASALRE
210 220 230 240 250
GCKDIATQLI SNMDINVILG GGRKYMFPAG TPDPEYPNDA NETGTRLDGR
260 270 280 290 300
NLVQEWLSKH QGSQYVWNRE QLIQKAQDPS VTYLMGLFEP VDTKFDIQRD
310 320 330 340 350
PLMDPSLKDM TETAVKVLSR NPKGFYLFVE GGRIDRGHHL GTAYLALTEA
360 370 380 390 400
VMFDLAIERA SQLTSERDTL TIVTADHSHV FSFGGYTLRG TSIFGLAPLN
410 420 430 440 450
ALDGKPYTSI LYGNGPGYVG TGERPNVTAA ESSGSSYRRQ AAVPVKSETH
460 470 480 490 500
GGEDVAIFAR GPQAHLVHGV QEQNYIAHVM ASAGCLEPYT DCGLAPPADE
510 520 530 540 550
SQTTTTTRQT TITTTTTTTT TTTTPVHNSA RSLGPATAPL ALALLAGMLM

LLLGAPAES
Length:559
Mass (Da):60,285
Last modified:March 1, 1992 - v1
Checksum:i019EBB4363950878
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61705 Genomic DNA. Translation: AAA37873.1.
CCDSiCCDS15127.1.
PIRiB36307.
UniGeneiMm.377076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61705 Genomic DNA. Translation: AAA37873.1.
CCDSiCCDS15127.1.
PIRiB36307.
UniGeneiMm.377076.

3D structure databases

ProteinModelPortaliP24822.
SMRiP24822. Positions 20-500.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037497.

Chemistry

BindingDBiP24822.
ChEMBLiCHEMBL3151.

PTM databases

PhosphoSiteiP24822.

Proteomic databases

MaxQBiP24822.
PaxDbiP24822.
PRIDEiP24822.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:87984. Akp3.

Phylogenomic databases

eggNOGiCOG1785.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP24822.
PhylomeDBiP24822.

Miscellaneous databases

SOURCEiSearch...

Gene expression databases

GenevestigatoriP24822.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic structure and comparison of mouse tissue-specific alkaline phosphatase genes."
    Manes T., Glade K., Ziomek C.A., Millan J.L.
    Genomics 8:541-554(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPPBI_MOUSE
AccessioniPrimary (citable) accession number: P24822
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: January 7, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.