Reviewed, UniProtKB/Swiss-Prot P24822 (PPBI_MOUSE)

Names and origin

Protein names

Recommended name:
    Intestinal alkaline phosphatase
      Short name=IAP
    EC=3.1.3.1

Gene names

Name:	Iap
Synonyms:	Akp-3, Akp3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion By similarity. Binds 2 zinc ions By similarity.
Subunit structure	Homodimer.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Potential.
Tissue specificity	Intestine and thymus.
Miscellaneous	In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).
Sequence similarities	Belongs to the alkaline phosphatase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Magnesium Metal-binding Zinc
Molecular function	Hydrolase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro multicellular organism growth Inferred from mutant phenotype. Source: MGI neural tube development Inferred from mutant phenotype. Source: MGI neurogenesis Inferred from mutant phenotype. Source: MGI tail morphogenesis Inferred from mutant phenotype. Source: MGI
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alkaline phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 528	509	Intestinal alkaline phosphatase		PRO_0000024039
	Propeptide	529 – 559	31	Removed in mature form Potential		PRO_0000024040
Regions
	Compositional bias	503 – 507	5	Poly-Thr
	Compositional bias	510 – 524	15	Poly-Thr
Sites
	Active site	111	1	Phosphoserine intermediate
	Metal binding	61	1	Magnesium Potential
	Metal binding	61	1	Zinc 2 Potential
	Metal binding	330	1	Magnesium Potential
	Metal binding	335	1	Zinc 1 Potential
	Metal binding	339	1	Zinc 1 Potential
	Metal binding	376	1	Zinc 2 Potential
	Metal binding	377	1	Zinc 2 Potential
	Metal binding	450	1	Zinc 1 Potential
Amino acid modifications
	Modified residue	111	1	Phosphoserine By similarity
	Lipidation	528	1	GPI-anchor amidated asparagine Potential
	Glycosylation	141	1	N-linked (GlcNAc...) Potential
	Glycosylation	241	1	N-linked (GlcNAc...) Potential
	Glycosylation	426	1	N-linked (GlcNAc...) Potential
	Disulfide bond	140 ↔ 202		By similarity
	Disulfide bond	485 ↔ 492		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24822-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 019EBB4363950878	FASTA	559	60,285
	10 20 30 40 50 60 MQGPWVLLLL GLRLQLSLSV IPVEEENPAF WNKKAAEALD AAKKLQPIQT SAKNLIIFLG 70 80 90 100 110 120 DGMGVPTVTA TRILKGQLEG HLGPETPLAM DRFPYMALSK TYSVDRQVPD SASTATAYLC 130 140 150 160 170 180 GVKTNYKTIG LSAAARFDQC NTTFGNEVFS VMYRAKKAGK SVGVVTTTRV QHASPSGTYV 190 200 210 220 230 240 HTVNRNWYGD ADMPASALRE GCKDIATQLI SNMDINVILG GGRKYMFPAG TPDPEYPNDA 250 260 270 280 290 300 NETGTRLDGR NLVQEWLSKH QGSQYVWNRE QLIQKAQDPS VTYLMGLFEP VDTKFDIQRD 310 320 330 340 350 360 PLMDPSLKDM TETAVKVLSR NPKGFYLFVE GGRIDRGHHL GTAYLALTEA VMFDLAIERA 370 380 390 400 410 420 SQLTSERDTL TIVTADHSHV FSFGGYTLRG TSIFGLAPLN ALDGKPYTSI LYGNGPGYVG 430 440 450 460 470 480 TGERPNVTAA ESSGSSYRRQ AAVPVKSETH GGEDVAIFAR GPQAHLVHGV QEQNYIAHVM 490 500 510 520 530 540 ASAGCLEPYT DCGLAPPADE SQTTTTTRQT TITTTTTTTT TTTTPVHNSA RSLGPATAPL 550 ALALLAGMLM LLLGAPAES

References

[1]	"Genomic structure and comparison of mouse tissue-specific alkaline phosphatase genes." Manes T., Glade K., Ziomek C.A., Millan J.L. Genomics 8:541-554(1990) [PubMed: 2286375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M61705 Genomic DNA. Translation: AAA37873.1.
IPI	IPI00111121.
PIR	B36307.
UniGene	Mm.377076
3D structure databases
HSSP	HSSP built from PDB template 1EW2 based on UniProtKB P05187.
SMR	P24822. Positions 20-497.
ModBase	Search...
PTM databases
PhosphoSite	P24822.
Proteomic databases
PRIDE	P24822.
Genome annotation databases
Ensembl	ENSMUSG00000036500. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:87984. Akp3.
Phylogenomic databases
HOGENOM	P24822.
HOVERGEN	P24822.
Enzyme and pathway databases
BRENDA	3.1.3.1. 244.
Gene expression databases
ArrayExpress	P24822.
Bgee	P24822.
GermOnline	ENSMUSG00000036500. Mus musculus.
Family and domain databases
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR001952. Alkaline_phosphatase. IPR018299. Alkaline_phosphatase_AS. [Graphical view]
Gene3D	G3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
Pfam	PF00245. Alk_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00113. ALKPHPHTASE.
SMART	SM00098. alkPPc. 1 hit. [Graphical view]
PROSITE	PS00123. ALKALINE_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

PPBI_MOUSE

Accession

Primary (citable) accession number: P24822

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)