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Protein

Intestinal-type alkaline phosphatase

Gene

Iap

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61MagnesiumBy similarity1
Metal bindingi61Zinc 1By similarity1
Active sitei111Phosphoserine intermediate1
Metal bindingi111Zinc 1By similarity1
Metal bindingi174MagnesiumBy similarity1
Metal bindingi330MagnesiumBy similarity1
Metal bindingi335Zinc 2By similarity1
Metal bindingi339Zinc 2; via tele nitrogenBy similarity1
Metal bindingi376Zinc 1By similarity1
Metal bindingi377Zinc 1; via tele nitrogenBy similarity1
Metal bindingi450Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

  • dephosphorylation Source: UniProtKB
  • multicellular organism growth Source: MGI
  • neural tube development Source: MGI
  • neurogenesis Source: MGI
  • post-anal tail morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Intestinal-type alkaline phosphatase (EC:3.1.3.1)
Short name:
IAP
Short name:
Intestinal alkaline phosphatase
Alternative name(s):
Alkaline phosphatase 3
Gene namesi
Name:Iap
Synonyms:Akp-3, Akp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:87984. Akp3.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • brush border Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3151.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000002403920 – 528Intestinal-type alkaline phosphataseAdd BLAST509
PropeptideiPRO_0000024040529 – 559Removed in mature formSequence analysisAdd BLAST31

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi140 ↔ 202By similarity
Glycosylationi141N-linked (GlcNAc...)Sequence analysis1
Glycosylationi241N-linked (GlcNAc...)Sequence analysis1
Glycosylationi426N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi485 ↔ 492By similarity
Lipidationi528GPI-anchor amidated asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP24822.
PaxDbiP24822.
PeptideAtlasiP24822.
PRIDEiP24822.

PTM databases

iPTMnetiP24822.
PhosphoSitePlusiP24822.

Expressioni

Tissue specificityi

Intestine and thymus.

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037497.

Chemistry databases

BindingDBiP24822.

Structurei

3D structure databases

ProteinModelPortaliP24822.
SMRiP24822.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi503 – 507Poly-Thr5
Compositional biasi510 – 524Poly-ThrAdd BLAST15

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP24822.
PhylomeDBiP24822.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGPWVLLLL GLRLQLSLSV IPVEEENPAF WNKKAAEALD AAKKLQPIQT
60 70 80 90 100
SAKNLIIFLG DGMGVPTVTA TRILKGQLEG HLGPETPLAM DRFPYMALSK
110 120 130 140 150
TYSVDRQVPD SASTATAYLC GVKTNYKTIG LSAAARFDQC NTTFGNEVFS
160 170 180 190 200
VMYRAKKAGK SVGVVTTTRV QHASPSGTYV HTVNRNWYGD ADMPASALRE
210 220 230 240 250
GCKDIATQLI SNMDINVILG GGRKYMFPAG TPDPEYPNDA NETGTRLDGR
260 270 280 290 300
NLVQEWLSKH QGSQYVWNRE QLIQKAQDPS VTYLMGLFEP VDTKFDIQRD
310 320 330 340 350
PLMDPSLKDM TETAVKVLSR NPKGFYLFVE GGRIDRGHHL GTAYLALTEA
360 370 380 390 400
VMFDLAIERA SQLTSERDTL TIVTADHSHV FSFGGYTLRG TSIFGLAPLN
410 420 430 440 450
ALDGKPYTSI LYGNGPGYVG TGERPNVTAA ESSGSSYRRQ AAVPVKSETH
460 470 480 490 500
GGEDVAIFAR GPQAHLVHGV QEQNYIAHVM ASAGCLEPYT DCGLAPPADE
510 520 530 540 550
SQTTTTTRQT TITTTTTTTT TTTTPVHNSA RSLGPATAPL ALALLAGMLM

LLLGAPAES
Length:559
Mass (Da):60,285
Last modified:March 1, 1992 - v1
Checksum:i019EBB4363950878
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61705 Genomic DNA. Translation: AAA37873.1.
CCDSiCCDS15127.1.
PIRiB36307.
UniGeneiMm.377076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61705 Genomic DNA. Translation: AAA37873.1.
CCDSiCCDS15127.1.
PIRiB36307.
UniGeneiMm.377076.

3D structure databases

ProteinModelPortaliP24822.
SMRiP24822.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037497.

Chemistry databases

BindingDBiP24822.
ChEMBLiCHEMBL3151.

PTM databases

iPTMnetiP24822.
PhosphoSitePlusiP24822.

Proteomic databases

MaxQBiP24822.
PaxDbiP24822.
PeptideAtlasiP24822.
PRIDEiP24822.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:87984. Akp3.

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP24822.
PhylomeDBiP24822.

Miscellaneous databases

PROiP24822.
SOURCEiSearch...

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPBI_MOUSE
AccessioniPrimary (citable) accession number: P24822
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.