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P24815

- 3BHS1_MOUSE

UniProt

P24815 - 3BHS1_MOUSE

Protein

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Gene

Hsd3b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

    Catalytic activityi

    A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH.
    A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei155 – 1551Proton acceptorBy similarity
    Binding sitei159 – 1591NADBy similarity

    GO - Molecular functioni

    1. 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: UniProtKB-EC
    2. steroid delta-isomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. steroid biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    ReactomeiREACT_204887. Mineralocorticoid biosynthesis.
    REACT_207160. Glucocorticoid biosynthesis.
    REACT_220332. Androgen biosynthesis.
    SABIO-RKP24815.
    UniPathwayiUPA00062.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
    Alternative name(s):
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
    Short name:
    3-beta-HSD I
    Including the following 2 domains:
    3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)
    Alternative name(s):
    3-beta-hydroxy-5-ene steroid dehydrogenase
    Progesterone reductase
    Steroid Delta-isomerase (EC:5.3.3.1)
    Alternative name(s):
    Delta-5-3-ketosteroid isomerase
    Gene namesi
    Name:Hsd3b1
    Synonyms:Hsd3b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:96233. Hsd3b1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. mitochondrial membrane Source: UniProtKB-SubCell
    4. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 3733723 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1PRO_0000087780Add
    BLAST

    Proteomic databases

    MaxQBiP24815.
    PaxDbiP24815.
    PRIDEiP24815.

    PTM databases

    PhosphoSiteiP24815.

    Expressioni

    Tissue specificityi

    Steroidogenic tissues (includes testes, ovaries and adrenal glands).

    Gene expression databases

    BgeeiP24815.
    GenevestigatoriP24815.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000102630.

    Structurei

    3D structure databases

    ProteinModelPortaliP24815.
    SMRiP24815. Positions 7-234.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei288 – 30821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the 3-beta-HSD family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0451.
    HOVERGENiHBG000014.
    InParanoidiP24815.
    KOiK00070.
    OMAiMAGWSCL.
    TreeFamiTF343138.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01073. 3Beta_HSD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24815-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGWSCLVTG AGGFVGQRII KMLVQEKELQ EVRALDKVFR PETKEEFSKL    50
    QTKTKVTVLE GDILDAQCLR RACQGISVVI HTAAVIDVTG VIPRQTILDV 100
    NLKGTQNLLE ACVQASVPAF IFCSSVDVAG PNSYKKIVLN GHEEQNHEST 150
    WSDPYPYSKK MAEKAVLAAN GSMLKNGGTL NTCALRPMYI YGERSPFIFN 200
    AIIRALKNKG ILCVTGKFSI ANPVYVENVA WAHILAARGL RDPKKSTSIQ 250
    GQFYYISDDT PHQSYDDLNY TLSKEWGLRP NASWSLPLPL LYWLAFLLET 300
    VSFLLRPVYR YRPLFNRHLI TLSNSTFTFS YKKAQRDLGY EPLVNWEEAK 350
    QKTSEWIGTI VEQHREILDT KCQ 373
    Length:373
    Mass (Da):42,062
    Last modified:January 23, 2007 - v3
    Checksum:i395F9D786C7BA010
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti128 – 1281V → A in AAH52659. (PubMed:15489334)Curated
    Sequence conflicti319 – 3191L → S in AAH52659. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58567 mRNA. Translation: AAA37860.1.
    BC052659 mRNA. Translation: AAH52659.1.
    CCDSiCCDS17670.1.
    PIRiI49762.
    RefSeqiNP_032319.1. NM_008293.3.
    XP_006501098.1. XM_006501035.1.
    XP_006501099.1. XM_006501036.1.
    XP_006501100.1. XM_006501037.1.
    UniGeneiMm.140811.

    Genome annotation databases

    EnsembliENSMUST00000107016; ENSMUSP00000102630; ENSMUSG00000027871.
    GeneIDi15492.
    KEGGimmu:15492.
    UCSCiuc008qqh.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58567 mRNA. Translation: AAA37860.1 .
    BC052659 mRNA. Translation: AAH52659.1 .
    CCDSi CCDS17670.1.
    PIRi I49762.
    RefSeqi NP_032319.1. NM_008293.3.
    XP_006501098.1. XM_006501035.1.
    XP_006501099.1. XM_006501036.1.
    XP_006501100.1. XM_006501037.1.
    UniGenei Mm.140811.

    3D structure databases

    ProteinModelPortali P24815.
    SMRi P24815. Positions 7-234.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000102630.

    PTM databases

    PhosphoSitei P24815.

    Proteomic databases

    MaxQBi P24815.
    PaxDbi P24815.
    PRIDEi P24815.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000107016 ; ENSMUSP00000102630 ; ENSMUSG00000027871 .
    GeneIDi 15492.
    KEGGi mmu:15492.
    UCSCi uc008qqh.1. mouse.

    Organism-specific databases

    CTDi 3283.
    MGIi MGI:96233. Hsd3b1.

    Phylogenomic databases

    eggNOGi COG0451.
    HOVERGENi HBG000014.
    InParanoidi P24815.
    KOi K00070.
    OMAi MAGWSCL.
    TreeFami TF343138.

    Enzyme and pathway databases

    UniPathwayi UPA00062 .
    Reactomei REACT_204887. Mineralocorticoid biosynthesis.
    REACT_207160. Glucocorticoid biosynthesis.
    REACT_220332. Androgen biosynthesis.
    SABIO-RK P24815.

    Miscellaneous databases

    NextBioi 288366.
    PROi P24815.
    SOURCEi Search...

    Gene expression databases

    Bgeei P24815.
    Genevestigatori P24815.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002225. 3Beta_OHSteriod_DH/Estase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01073. 3Beta_HSD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Multiple forms of mouse 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase and differential expression in gonads, adrenal glands, liver, and kidneys of both sexes."
      Bain P.A., Yoo M., Clarke T., Hammond S.H., Payne A.H.
      Proc. Natl. Acad. Sci. U.S.A. 88:8870-8874(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: CD-1.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Egg.

    Entry informationi

    Entry namei3BHS1_MOUSE
    AccessioniPrimary (citable) accession number: P24815
    Secondary accession number(s): Q7TQ00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 123 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3