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Protein

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Gene

Hsd3b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Catalytic activityi

A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH.
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

Pathway:isteroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei155 – 1551Proton acceptorBy similarity
Binding sitei159 – 1591NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_295803. Mineralocorticoid biosynthesis.
REACT_297756. Glucocorticoid biosynthesis.
REACT_305222. Androgen biosynthesis.
SABIO-RKP24815.
UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
Short name:
3-beta-HSD I
Including the following 2 domains:
3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)
Alternative name(s):
3-beta-hydroxy-5-ene steroid dehydrogenase
Progesterone reductase
Steroid Delta-isomerase (EC:5.3.3.1)
Alternative name(s):
Delta-5-3-ketosteroid isomerase
Gene namesi
Name:Hsd3b1
Synonyms:Hsd3b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:96233. Hsd3b1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei288 – 30821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 3733723 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1PRO_0000087780Add
BLAST

Proteomic databases

MaxQBiP24815.
PaxDbiP24815.
PRIDEiP24815.

PTM databases

PhosphoSiteiP24815.

Expressioni

Tissue specificityi

Steroidogenic tissues (includes testes, ovaries and adrenal glands).

Gene expression databases

BgeeiP24815.
ExpressionAtlasiP24815. baseline and differential.
GenevisibleiP24815. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102630.

Structurei

3D structure databases

ProteinModelPortaliP24815.
SMRiP24815. Positions 4-129.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0451.
GeneTreeiENSGT00550000074557.
HOVERGENiHBG000014.
InParanoidiP24815.
KOiK00070.
OMAiDSRMSLP.
TreeFamiTF343138.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24815-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGWSCLVTG AGGFVGQRII KMLVQEKELQ EVRALDKVFR PETKEEFSKL
60 70 80 90 100
QTKTKVTVLE GDILDAQCLR RACQGISVVI HTAAVIDVTG VIPRQTILDV
110 120 130 140 150
NLKGTQNLLE ACVQASVPAF IFCSSVDVAG PNSYKKIVLN GHEEQNHEST
160 170 180 190 200
WSDPYPYSKK MAEKAVLAAN GSMLKNGGTL NTCALRPMYI YGERSPFIFN
210 220 230 240 250
AIIRALKNKG ILCVTGKFSI ANPVYVENVA WAHILAARGL RDPKKSTSIQ
260 270 280 290 300
GQFYYISDDT PHQSYDDLNY TLSKEWGLRP NASWSLPLPL LYWLAFLLET
310 320 330 340 350
VSFLLRPVYR YRPLFNRHLI TLSNSTFTFS YKKAQRDLGY EPLVNWEEAK
360 370
QKTSEWIGTI VEQHREILDT KCQ
Length:373
Mass (Da):42,062
Last modified:January 23, 2007 - v3
Checksum:i395F9D786C7BA010
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti128 – 1281V → A in AAH52659 (PubMed:15489334).Curated
Sequence conflicti319 – 3191L → S in AAH52659 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58567 mRNA. Translation: AAA37860.1.
BC052659 mRNA. Translation: AAH52659.1.
CCDSiCCDS17670.1.
PIRiI49762.
RefSeqiNP_001291729.1. NM_001304800.1.
NP_032319.1. NM_008293.4.
XP_006501099.1. XM_006501036.1.
UniGeneiMm.140811.

Genome annotation databases

EnsembliENSMUST00000029465; ENSMUSP00000029465; ENSMUSG00000027871.
ENSMUST00000107016; ENSMUSP00000102630; ENSMUSG00000027871.
GeneIDi15492.
KEGGimmu:15492.
UCSCiuc008qqh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58567 mRNA. Translation: AAA37860.1.
BC052659 mRNA. Translation: AAH52659.1.
CCDSiCCDS17670.1.
PIRiI49762.
RefSeqiNP_001291729.1. NM_001304800.1.
NP_032319.1. NM_008293.4.
XP_006501099.1. XM_006501036.1.
UniGeneiMm.140811.

3D structure databases

ProteinModelPortaliP24815.
SMRiP24815. Positions 4-129.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102630.

PTM databases

PhosphoSiteiP24815.

Proteomic databases

MaxQBiP24815.
PaxDbiP24815.
PRIDEiP24815.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029465; ENSMUSP00000029465; ENSMUSG00000027871.
ENSMUST00000107016; ENSMUSP00000102630; ENSMUSG00000027871.
GeneIDi15492.
KEGGimmu:15492.
UCSCiuc008qqh.2. mouse.

Organism-specific databases

CTDi3283.
MGIiMGI:96233. Hsd3b1.

Phylogenomic databases

eggNOGiCOG0451.
GeneTreeiENSGT00550000074557.
HOVERGENiHBG000014.
InParanoidiP24815.
KOiK00070.
OMAiDSRMSLP.
TreeFamiTF343138.

Enzyme and pathway databases

UniPathwayiUPA00062.
ReactomeiREACT_295803. Mineralocorticoid biosynthesis.
REACT_297756. Glucocorticoid biosynthesis.
REACT_305222. Androgen biosynthesis.
SABIO-RKP24815.

Miscellaneous databases

NextBioi288366.
PROiP24815.
SOURCEiSearch...

Gene expression databases

BgeeiP24815.
ExpressionAtlasiP24815. baseline and differential.
GenevisibleiP24815. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple forms of mouse 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase and differential expression in gonads, adrenal glands, liver, and kidneys of both sexes."
    Bain P.A., Yoo M., Clarke T., Hammond S.H., Payne A.H.
    Proc. Natl. Acad. Sci. U.S.A. 88:8870-8874(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD-1.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Egg.

Entry informationi

Entry namei3BHS1_MOUSE
AccessioniPrimary (citable) accession number: P24815
Secondary accession number(s): Q7TQ00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.