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P24815

- 3BHS1_MOUSE

UniProt

P24815 - 3BHS1_MOUSE

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Protein

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Gene

Hsd3b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Catalytic activityi

A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH.
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei155 – 1551Proton acceptorBy similarity
Binding sitei159 – 1591NADBy similarity

GO - Molecular functioni

  1. 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: UniProtKB-EC
  2. steroid delta-isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. steroid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_204887. Mineralocorticoid biosynthesis.
REACT_207160. Glucocorticoid biosynthesis.
REACT_220332. Androgen biosynthesis.
SABIO-RKP24815.
UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
Short name:
3-beta-HSD I
Including the following 2 domains:
3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)
Alternative name(s):
3-beta-hydroxy-5-ene steroid dehydrogenase
Progesterone reductase
Steroid Delta-isomerase (EC:5.3.3.1)
Alternative name(s):
Delta-5-3-ketosteroid isomerase
Gene namesi
Name:Hsd3b1
Synonyms:Hsd3b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:96233. Hsd3b1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 3733723 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1PRO_0000087780Add
BLAST

Proteomic databases

MaxQBiP24815.
PaxDbiP24815.
PRIDEiP24815.

PTM databases

PhosphoSiteiP24815.

Expressioni

Tissue specificityi

Steroidogenic tissues (includes testes, ovaries and adrenal glands).

Gene expression databases

BgeeiP24815.
ExpressionAtlasiP24815. baseline and differential.
GenevestigatoriP24815.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102630.

Structurei

3D structure databases

ProteinModelPortaliP24815.
SMRiP24815. Positions 7-234.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei288 – 30821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0451.
HOVERGENiHBG000014.
InParanoidiP24815.
KOiK00070.
OMAiMAGWSCL.
TreeFamiTF343138.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24815-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGWSCLVTG AGGFVGQRII KMLVQEKELQ EVRALDKVFR PETKEEFSKL
60 70 80 90 100
QTKTKVTVLE GDILDAQCLR RACQGISVVI HTAAVIDVTG VIPRQTILDV
110 120 130 140 150
NLKGTQNLLE ACVQASVPAF IFCSSVDVAG PNSYKKIVLN GHEEQNHEST
160 170 180 190 200
WSDPYPYSKK MAEKAVLAAN GSMLKNGGTL NTCALRPMYI YGERSPFIFN
210 220 230 240 250
AIIRALKNKG ILCVTGKFSI ANPVYVENVA WAHILAARGL RDPKKSTSIQ
260 270 280 290 300
GQFYYISDDT PHQSYDDLNY TLSKEWGLRP NASWSLPLPL LYWLAFLLET
310 320 330 340 350
VSFLLRPVYR YRPLFNRHLI TLSNSTFTFS YKKAQRDLGY EPLVNWEEAK
360 370
QKTSEWIGTI VEQHREILDT KCQ
Length:373
Mass (Da):42,062
Last modified:January 23, 2007 - v3
Checksum:i395F9D786C7BA010
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti128 – 1281V → A in AAH52659. (PubMed:15489334)Curated
Sequence conflicti319 – 3191L → S in AAH52659. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58567 mRNA. Translation: AAA37860.1.
BC052659 mRNA. Translation: AAH52659.1.
CCDSiCCDS17670.1.
PIRiI49762.
RefSeqiNP_032319.1. NM_008293.3.
XP_006501098.1. XM_006501035.1.
XP_006501099.1. XM_006501036.1.
XP_006501100.1. XM_006501037.1.
UniGeneiMm.140811.

Genome annotation databases

EnsembliENSMUST00000107016; ENSMUSP00000102630; ENSMUSG00000027871.
GeneIDi15492.
KEGGimmu:15492.
UCSCiuc008qqh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58567 mRNA. Translation: AAA37860.1 .
BC052659 mRNA. Translation: AAH52659.1 .
CCDSi CCDS17670.1.
PIRi I49762.
RefSeqi NP_032319.1. NM_008293.3.
XP_006501098.1. XM_006501035.1.
XP_006501099.1. XM_006501036.1.
XP_006501100.1. XM_006501037.1.
UniGenei Mm.140811.

3D structure databases

ProteinModelPortali P24815.
SMRi P24815. Positions 7-234.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000102630.

PTM databases

PhosphoSitei P24815.

Proteomic databases

MaxQBi P24815.
PaxDbi P24815.
PRIDEi P24815.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000107016 ; ENSMUSP00000102630 ; ENSMUSG00000027871 .
GeneIDi 15492.
KEGGi mmu:15492.
UCSCi uc008qqh.1. mouse.

Organism-specific databases

CTDi 3283.
MGIi MGI:96233. Hsd3b1.

Phylogenomic databases

eggNOGi COG0451.
HOVERGENi HBG000014.
InParanoidi P24815.
KOi K00070.
OMAi MAGWSCL.
TreeFami TF343138.

Enzyme and pathway databases

UniPathwayi UPA00062 .
Reactomei REACT_204887. Mineralocorticoid biosynthesis.
REACT_207160. Glucocorticoid biosynthesis.
REACT_220332. Androgen biosynthesis.
SABIO-RK P24815.

Miscellaneous databases

NextBioi 288366.
PROi P24815.
SOURCEi Search...

Gene expression databases

Bgeei P24815.
ExpressionAtlasi P24815. baseline and differential.
Genevestigatori P24815.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF01073. 3Beta_HSD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple forms of mouse 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase and differential expression in gonads, adrenal glands, liver, and kidneys of both sexes."
    Bain P.A., Yoo M., Clarke T., Hammond S.H., Payne A.H.
    Proc. Natl. Acad. Sci. U.S.A. 88:8870-8874(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD-1.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Egg.

Entry informationi

Entry namei3BHS1_MOUSE
AccessioniPrimary (citable) accession number: P24815
Secondary accession number(s): Q7TQ00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3