Reviewed, UniProtKB/Swiss-Prot P24815 (3BHS1_MOUSE)

Names and origin

Protein names

Recommended name:
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
      Short name=3-beta-HSD I
Including the following 2 domains:
    1- Recommended name:
            3-beta-hydroxy-Delta(5)-steroid dehydrogenase
              EC=1.1.1.145
        Alternative name(s):
            3-beta-hydroxy-5-ene steroid dehydrogenase
            Progesterone reductase
    2- Recommended name:
            Steroid Delta-isomerase
              EC=5.3.3.1
        Alternative name(s):
            Delta-5-3-ketosteroid isomerase

Gene names

Name:	Hsd3b1
Synonyms:	Hsd3b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Catalytic activity	A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
Tissue specificity	Steroidogenic tissues (includes testes, ovaries and adrenal glands).
Sequence similarities	Belongs to the 3-beta-HSD family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Domain	Transmembrane
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 373	372	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1		PRO_0000087780
Regions
	Transmembrane	288 – 308	21	Potential
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	159	1	NAD By similarity
Experimental info
	Sequence conflict	128	1	V → A in AAH52659. Ref.2
	Sequence conflict	319	1	L → S in AAH52659. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P24815-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 395F9D786C7BA010	FASTA	373	42,062
	10 20 30 40 50 60 MAGWSCLVTG AGGFVGQRII KMLVQEKELQ EVRALDKVFR PETKEEFSKL QTKTKVTVLE 70 80 90 100 110 120 GDILDAQCLR RACQGISVVI HTAAVIDVTG VIPRQTILDV NLKGTQNLLE ACVQASVPAF 130 140 150 160 170 180 IFCSSVDVAG PNSYKKIVLN GHEEQNHEST WSDPYPYSKK MAEKAVLAAN GSMLKNGGTL 190 200 210 220 230 240 NTCALRPMYI YGERSPFIFN AIIRALKNKG ILCVTGKFSI ANPVYVENVA WAHILAARGL 250 260 270 280 290 300 RDPKKSTSIQ GQFYYISDDT PHQSYDDLNY TLSKEWGLRP NASWSLPLPL LYWLAFLLET 310 320 330 340 350 360 VSFLLRPVYR YRPLFNRHLI TLSNSTFTFS YKKAQRDLGY EPLVNWEEAK QKTSEWIGTI 370 VEQHREILDT KCQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multiple forms of mouse 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase and differential expression in gonads, adrenal glands, liver, and kidneys of both sexes." Bain P.A., Yoo M., Clarke T., Hammond S.H., Payne A.H. Proc. Natl. Acad. Sci. U.S.A. 88:8870-8874(1991) [PubMed: 1924345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CD-1.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Egg.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M58567 mRNA. Translation: AAA37860.1. BC052659 mRNA. Translation: AAH52659.1.
IPI	IPI00229070.
PIR	I49762.
RefSeq	NP_032319.1.
UniGene	Mm.140811
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P24815.
Genome annotation databases
Ensembl	ENSMUSG00000027871. Mus musculus. [Contig view]
GeneID	15492.
KEGG	mmu:15492.
Organism-specific databases
MGI	MGI:96233. Hsd3b1.
Phylogenomic databases
HOVERGEN	P24815.
OMA	P24815. LQDPKKA.
Enzyme and pathway databases
BRENDA	1.1.1.145. 244. 5.3.3.1. 244.
Gene expression databases
ArrayExpress	P24815.
Bgee	P24815.
GermOnline	ENSMUSG00000027871. Mus musculus.
Family and domain databases
InterPro	IPR002225. 3Beta_OHSteriod_DH/Estase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01073. 3Beta_HSD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	288366.
SOURCE	Search...

Entry information

Entry name

3BHS1_MOUSE

Accession

Primary (citable) accession number: P24815
Secondary accession number(s): Q7TQ00

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)