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P24814 (GRR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SCF E3 ubiquitin ligase complex F-box protein GRR1
Alternative name(s):
F-box and leucine-rich repeat protein GRR1
F-box/LRR-repeat protein GRR1
Gene names
Name:GRR1
Synonyms:CAT80, COT2
Ordered Locus Names:YJR090C
ORF Names:J1885
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1151 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and directs ubiquitination of phosphorylated CLN1, CLN2 and GIC2. Probably constitutes the primary response element required for the generation or interpretation of the signal that induces glucose repression. Ref.1 Ref.5 Ref.6 Ref.7

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with SKP1. Component of the probable SCF(GRR1) complex containing CDC53, SKP1, RBX1 and GRR1. Ref.7 Ref.8

Subcellular location

Membrane. Note: Associated with the particulate fraction. Probably forms a complex by protein-protein interactions via its leucine-rich segment. Ref.1

Induction

Expressed constitutively at low levels.

Sequence similarities

Contains 1 F-box domain.

Contains 13 LRR (leucine-rich) repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CDC53Q120188EBI-7898,EBI-4321
SKP1P522866EBI-7898,EBI-4090

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11511151SCF E3 ubiquitin ligase complex F-box protein GRR1
PRO_0000119968

Regions

Domain314 – 36148F-box
Repeat399 – 42325LRR 1
Repeat424 – 44926LRR 2
Repeat450 – 47526LRR 3
Repeat476 – 50126LRR 4
Repeat502 – 52726LRR 5
Repeat528 – 55326LRR 6
Repeat554 – 58229LRR 7
Repeat583 – 60826LRR 8
Repeat609 – 63426LRR 9
Repeat635 – 66026LRR 10
Repeat661 – 68525LRR 11
Repeat686 – 71429LRR 12
Repeat715 – 74026LRR 13
Compositional bias38 – 4912Poly-Asn
Compositional bias1045 – 112480Asn-rich

Amino acid modifications

Modified residue1991Phosphoserine Ref.9
Modified residue3001Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P24814 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 6BB6C46611E6F825

FASTA1,151132,734
        10         20         30         40         50         60 
MDQDNNNHND SNRLHPPDIH PNLGPQLWLN SSGDFDDNNN NNNNNNNNNS TRPQMPSRTR 

        70         80         90        100        110        120 
ETATSERNAS EVRDATLNNI FRFDSIQRET LLPTNNGQPL NQNFSLTFQP QQQTNALNGI 

       130        140        150        160        170        180 
DINTVNTNLM NGVNVQIDQL NRLLPNLPEE ERKQIHEFKL IVGKKIQEFL VVIEKRRKKI 

       190        200        210        220        230        240 
LNEIELDNLK LKELRIDNSP QAISYLHKLQ RMRLRALETE NMEIRNLRLK ILTIIEEYKK 

       250        260        270        280        290        300 
SLYAYCHSKL RGQQVENPTD NFIIWINSID TTESSDLKEG LQDLSRYSRQ FINNVLSNPS 

       310        320        330        340        350        360 
NQNICTSVTR RSPVFALNML PSEILHLILD KLNQKYDIVK FLTVSKLWAE IIVKILYYRP 

       370        380        390        400        410        420 
HINKKSQLDL FLRTMKLTSE ETVFNYRLMI KRLNFSFVGD YMHDTELNYF VGCKNLERLT 

       430        440        450        460        470        480 
LVFCKHITSV PISAVLRGCK FLQSVDITGI RDVSDDVFDT LATYCPRVQG FYVPQARNVT 

       490        500        510        520        530        540 
FDSLRNFIVH SPMLKRIKIT ANNNMNDELV ELLANKCPLL VEVDITLSPN VTDSSLLKLL 

       550        560        570        580        590        600 
TRLVQLREFR ITHNTNITDN LFQELSKVVD DMPSLRLIDL SGCENITDKT IESIVNLAPK 

       610        620        630        640        650        660 
LRNVFLGKCS RITDASLFQL SKLGKNLQTV HFGHCFNITD NGVRALFHSC TRIQYVDFAC 

       670        680        690        700        710        720 
CTNLTNRTLY ELADLPKLKR IGLVKCTQMT DEGLLNMVSL RGRNDTLERV HLSYCSNLTI 

       730        740        750        760        770        780 
YPIYELLMSC PRLSHLSLTA VPSFLRPDIT MYCRPAPSDF SENQRQIFCV FSGKGVHKLR 

       790        800        810        820        830        840 
HYLVNLTSPA FGPHVDVNDV LTKYIRSKNL IFNGETLEDA LRRIITDLNQ DSAAIIAATG 

       850        860        870        880        890        900 
LNQINGLNND FLFQNINFER IDEVFSWYLN TFDGIRMSSE EVNSLLLQVN KTFCEDPFSD 

       910        920        930        940        950        960 
VDDDQDYVVA PGVNREINSE MCHIVRKFHE LNDHIDDFEV NVASLVRVQF QFTGFLLHEM 

       970        980        990       1000       1010       1020 
TQTYMQMIEL NRQICLVQKT VQESGNIDYQ KGLLIWRLLF IDKFIMVVQK YKLSTVVLRL 

      1030       1040       1050       1060       1070       1080 
YLKDNITLLT RQRELLIAHQ RSAWNNNNDN DANRNANNIV NIVSDAGAND TSNNETNNGN 

      1090       1100       1110       1120       1130       1140 
DDNETENPNF WRQFGNRMQI SPDQMRNLQM GLRNQNMVRN NNNNTIDESM PDTAIDSQMD 

      1150 
EASGTPDEDM L 

« Hide

References

« Hide 'large scale' references
[1]"GRR1 of Saccharomyces cerevisiae is required for glucose repression and encodes a protein with leucine-rich repeats."
Flick J.S., Johnston M.
Mol. Cell. Biol. 11:5101-5112(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, LEUCINE-RICH REPEATS.
[2]"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
Huang M.-E., Manus V., Chuat J.-C., Galibert F.
Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"G1 cyclin turnover and nutrient uptake are controlled by a common pathway in yeast."
Barral Y., Jentsch S., Mann C.
Genes Dev. 9:399-409(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN UBIQUITINATION OF CLN1 AND CLN2.
[6]"The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex."
Jaquenoud M., Gulli M.P., Peter K., Peter M.
EMBO J. 17:5360-5373(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN UBIQUITINATION OF GIC2.
[7]"Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1."
Skowyra D., Koepp D.M., Kamura T., Conrad M.N., Conaway R.C., Conaway J.W., Elledge S.J., Harper J.W.
Science 284:662-665(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN SCF COMPLEX, FUNCTION IN UBIQUITINATION OF CLN1.
[8]"Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro."
Kus B.M., Caldon C.E., Andorn-Broza R., Edwards A.M.
Proteins 54:455-467(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SKP1, RECONSTITUTION OF THE SCF(GRR1) COMPLEX.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-300, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M59247 Genomic DNA. Translation: AAA34652.1.
Z49590 Genomic DNA. Translation: CAA89617.1.
L47993 Genomic DNA. Translation: AAB39313.1.
BK006943 Genomic DNA. Translation: DAA08874.1.
PIRA41529.
RefSeqNP_012623.1. NM_001181747.1.

3D structure databases

ProteinModelPortalP24814.
SMRP24814. Positions 413-450, 573-754, 1096-1128.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33844. 81 interactions.
DIPDIP-1626N.
IntActP24814. 8 interactions.
MINTMINT-396112.
STRING4932.YJR090C.

Proteomic databases

PaxDbP24814.
PeptideAtlasP24814.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR090C; YJR090C; YJR090C.
GeneID853552.
KEGGsce:YJR090C.

Organism-specific databases

CYGDYJR090c.
SGDS000003850. GRR1.

Phylogenomic databases

eggNOGNOG300245.
GeneTreeENSGT00750000117590.
HOGENOMHOG000112822.
KOK03360.
OMAGHCFNIT.
OrthoDBEOG7V76FS.

Enzyme and pathway databases

BioCycYEAST:G3O-31717-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorP24814.

Family and domain databases

InterProIPR001810. F-box_dom.
[Graphical view]
SMARTSM00256. FBOX. 1 hit.
[Graphical view]
PROSITEPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974287.

Entry information

Entry nameGRR1_YEAST
AccessionPrimary (citable) accession number: P24814
Secondary accession number(s): D6VWQ8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: April 16, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways