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Reviewed, UniProtKB/Swiss-Prot P24814 (GRR1_YEAST)

Last modified June 16, 2009. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    SCF E3 ubiquitin ligase complex F-box protein GRR1
Alternative name(s):
    F-box and leucine-rich repeat protein GRR1
    F-box/LRR-repeat protein GRR1
Gene names
Name: GRR1
Synonyms: CAT80, COT2
Ordered Locus Names: YJR090C
ORF Names: J1885
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1151 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and directs ubiquitination of phosphorylated CLN1, CLN2 and GIC2. Probably constitutes the primary response element required for the generation or interpretation of the signal that induces glucose repression. Ref.1 Ref.4 Ref.5 Ref.6

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with SKP1. Component of the probable SCF(GRR1) complex containing CDC53, SKP1, RBX1 and GRR1. Ref.7

Subcellular location

Membrane. Note: Associated with the particulate fraction. Probably forms a complex by protein-protein interactions via its leucine-rich segment. Ref.1

Induction

Expressed constitutively at low levels.

Sequence similarities

Contains 1 F-box domain.

Contains 12 LRR (leucine-rich) repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SKP1P522862EBI-7898,EBI-4090

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11511151SCF E3 ubiquitin ligase complex F-box protein GRR1
PRO_0000119968

Regions

Domain314 – 36148F-box
Repeat409 – 43426LRR 1
Repeat435 – 46026LRR 2
Repeat461 – 48626LRR 3
Repeat487 – 51226LRR 4
Repeat513 – 54836LRR 5
Repeat549 – 56719LRR 6
Repeat568 – 59326LRR 7
Repeat594 – 61926LRR 8
Repeat620 – 64526LRR 9
Repeat646 – 67025LRR 10
Repeat671 – 69929LRR 11
Repeat700 – 72627LRR 12
Compositional bias38 – 4912Poly-Asn
Compositional bias1045 – 112480Asn-rich

Amino acid modifications

Modified residue1991Phosphoserine Ref.8 Ref.9
Modified residue3001Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P24814-1 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 6BB6C46611E6F825

FASTA1,151132,734
        10         20         30         40         50         60 
MDQDNNNHND SNRLHPPDIH PNLGPQLWLN SSGDFDDNNN NNNNNNNNNS TRPQMPSRTR 

        70         80         90        100        110        120 
ETATSERNAS EVRDATLNNI FRFDSIQRET LLPTNNGQPL NQNFSLTFQP QQQTNALNGI 

       130        140        150        160        170        180 
DINTVNTNLM NGVNVQIDQL NRLLPNLPEE ERKQIHEFKL IVGKKIQEFL VVIEKRRKKI 

       190        200        210        220        230        240 
LNEIELDNLK LKELRIDNSP QAISYLHKLQ RMRLRALETE NMEIRNLRLK ILTIIEEYKK 

       250        260        270        280        290        300 
SLYAYCHSKL RGQQVENPTD NFIIWINSID TTESSDLKEG LQDLSRYSRQ FINNVLSNPS 

       310        320        330        340        350        360 
NQNICTSVTR RSPVFALNML PSEILHLILD KLNQKYDIVK FLTVSKLWAE IIVKILYYRP 

       370        380        390        400        410        420 
HINKKSQLDL FLRTMKLTSE ETVFNYRLMI KRLNFSFVGD YMHDTELNYF VGCKNLERLT 

       430        440        450        460        470        480 
LVFCKHITSV PISAVLRGCK FLQSVDITGI RDVSDDVFDT LATYCPRVQG FYVPQARNVT 

       490        500        510        520        530        540 
FDSLRNFIVH SPMLKRIKIT ANNNMNDELV ELLANKCPLL VEVDITLSPN VTDSSLLKLL 

       550        560        570        580        590        600 
TRLVQLREFR ITHNTNITDN LFQELSKVVD DMPSLRLIDL SGCENITDKT IESIVNLAPK 

       610        620        630        640        650        660 
LRNVFLGKCS RITDASLFQL SKLGKNLQTV HFGHCFNITD NGVRALFHSC TRIQYVDFAC 

       670        680        690        700        710        720 
CTNLTNRTLY ELADLPKLKR IGLVKCTQMT DEGLLNMVSL RGRNDTLERV HLSYCSNLTI 

       730        740        750        760        770        780 
YPIYELLMSC PRLSHLSLTA VPSFLRPDIT MYCRPAPSDF SENQRQIFCV FSGKGVHKLR 

       790        800        810        820        830        840 
HYLVNLTSPA FGPHVDVNDV LTKYIRSKNL IFNGETLEDA LRRIITDLNQ DSAAIIAATG 

       850        860        870        880        890        900 
LNQINGLNND FLFQNINFER IDEVFSWYLN TFDGIRMSSE EVNSLLLQVN KTFCEDPFSD 

       910        920        930        940        950        960 
VDDDQDYVVA PGVNREINSE MCHIVRKFHE LNDHIDDFEV NVASLVRVQF QFTGFLLHEM 

       970        980        990       1000       1010       1020 
TQTYMQMIEL NRQICLVQKT VQESGNIDYQ KGLLIWRLLF IDKFIMVVQK YKLSTVVLRL 

      1030       1040       1050       1060       1070       1080 
YLKDNITLLT RQRELLIAHQ RSAWNNNNDN DANRNANNIV NIVSDAGAND TSNNETNNGN 

      1090       1100       1110       1120       1130       1140 
DDNETENPNF WRQFGNRMQI SPDQMRNLQM GLRNQNMVRN NNNNTIDESM PDTAIDSQMD 

      1150 
EASGTPDEDM L 

« Hide

References

« Hide 'large scale' references
[1]"GRR1 of Saccharomyces cerevisiae is required for glucose repression and encodes a protein with leucine-rich repeats."
Flick J.S., Johnston M.
Mol. Cell. Biol. 11:5101-5112(1991) [PubMed: 1922034] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, LEUCINE-RICH REPEATS.
[2]"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
Huang M.-E., Manus V., Chuat J.-C., Galibert F.
Yeast 12:869-875(1996) [PubMed: 8840504] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"G1 cyclin turnover and nutrient uptake are controlled by a common pathway in yeast."
Barral Y., Jentsch S., Mann C.
Genes Dev. 9:399-409(1995) [PubMed: 7883165] [Abstract]
Cited for: FUNCTION IN UBIQUITINATION OF CLN1 AND CLN2.
[5]"The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex."
Jaquenoud M., Gulli M.P., Peter K., Peter M.
EMBO J. 17:5360-5373(1998) [PubMed: 9736614] [Abstract]
Cited for: FUNCTION IN UBIQUITINATION OF GIC2.
[6]"Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1."
Skowyra D., Koepp D.M., Kamura T., Conrad M.N., Conaway R.C., Conaway J.W., Elledge S.J., Harper J.W.
Science 284:662-665(1999) [PubMed: 10213692] [Abstract]
Cited for: IDENTIFICATION IN SCF COMPLEX, FUNCTION IN UBIQUITINATION OF CLN1.
[7]"Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro."
Kus B.M., Caldon C.E., Andorn-Broza R., Edwards A.M.
Proteins 54:455-467(2004) [PubMed: 14747994] [Abstract]
Cited for: INTERACTION WITH SKP1, RECONSTITUTION OF THE SCF(GRR1) COMPLEX.
[8]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, MASS SPECTROMETRY.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-300, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

M59247 Genomic DNA. Translation: AAA34652.1.
Z49590 Genomic DNA. Translation: CAA89617.1.
L47993 Genomic DNA. Translation: AAB39313.1.
PIRA41529.
RefSeqNP_012623.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:1626N.
IntActP24814. 18 interactions.

Proteomic databases

PeptideAtlasP24814.
PRIDEP24814.

Genome annotation databases

EnsemblYJR090C. Saccharomyces cerevisiae. [Contig view]
GeneID853552.
GenomeReviewsGene locus YJR090C in contig Y13136_GR.
KEGGsce:YJR090C.
NMPDRfig|4932.3.peg.3597.

Organism-specific databases

CYGDYJR090c.
SGDS000003850. GRR1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP24814.
OMAP24814. CPLLVEV.

Gene expression databases

ArrayExpressP24814.
GermOnlineYJR090C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001810. F-box.
IPR001611. Leu-rich_rpt.
[Graphical view]
PfamPF00646. F-box. 1 hit.
PF00560. LRR_1. 1 hit.
[Graphical view]
SMARTSM00256. FBOX. 1 hit.
[Graphical view]
PROSITEPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio974287.

Entry information

Entry nameGRR1_YEAST
AccessionPrimary (citable) accession number: P24814
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 16, 2009
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents