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P24814

- GRR1_YEAST

UniProt

P24814 - GRR1_YEAST

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Protein
SCF E3 ubiquitin ligase complex F-box protein GRR1
Gene
GRR1, CAT80, COT2, YJR090C, J1885
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and directs ubiquitination of phosphorylated CLN1, CLN2 and GIC2. Probably constitutes the primary response element required for the generation or interpretation of the signal that induces glucose repression.4 Publications

Pathwayi

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein binding, bridging Source: SGD

GO - Biological processi

  1. G1/S transition of mitotic cell cycle Source: SGD
  2. SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  3. cell cycle arrest in response to pheromone Source: SGD
  4. cellular response to methylmercury Source: SGD
  5. glucose metabolic process Source: UniProtKB-KW
  6. protein polyubiquitination Source: SGD
  7. protein ubiquitination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-31717-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
SCF E3 ubiquitin ligase complex F-box protein GRR1
Alternative name(s):
F-box and leucine-rich repeat protein GRR1
F-box/LRR-repeat protein GRR1
Gene namesi
Name:GRR1
Synonyms:CAT80, COT2
Ordered Locus Names:YJR090C
ORF Names:J1885
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR090c.
SGDiS000003850. GRR1.

Subcellular locationi

Membrane
Note: Associated with the particulate fraction. Probably forms a complex by protein-protein interactions via its leucine-rich segment.1 Publication

GO - Cellular componenti

  1. SCF ubiquitin ligase complex Source: SGD
  2. cellular bud neck contractile ring Source: SGD
  3. cytoplasm Source: SGD
  4. membrane Source: UniProtKB-SubCell
  5. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11511151SCF E3 ubiquitin ligase complex F-box protein GRR1
PRO_0000119968Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991Phosphoserine1 Publication
Modified residuei300 – 3001Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP24814.
PaxDbiP24814.
PeptideAtlasiP24814.

Expressioni

Inductioni

Expressed constitutively at low levels.

Gene expression databases

GenevestigatoriP24814.

Interactioni

Subunit structurei

Interacts with SKP1. Component of the probable SCF(GRR1) complex containing CDC53, SKP1, RBX1 and GRR1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC53Q120188EBI-7898,EBI-4321
SKP1P522866EBI-7898,EBI-4090

Protein-protein interaction databases

BioGridi33844. 90 interactions.
DIPiDIP-1626N.
IntActiP24814. 8 interactions.
MINTiMINT-396112.
STRINGi4932.YJR090C.

Structurei

3D structure databases

ProteinModelPortaliP24814.
SMRiP24814. Positions 483-753.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini314 – 36148F-box
Add
BLAST
Repeati399 – 42325LRR 1
Add
BLAST
Repeati424 – 44926LRR 2
Add
BLAST
Repeati450 – 47526LRR 3
Add
BLAST
Repeati476 – 50126LRR 4
Add
BLAST
Repeati502 – 52726LRR 5
Add
BLAST
Repeati528 – 55326LRR 6
Add
BLAST
Repeati554 – 58229LRR 7
Add
BLAST
Repeati583 – 60826LRR 8
Add
BLAST
Repeati609 – 63426LRR 9
Add
BLAST
Repeati635 – 66026LRR 10
Add
BLAST
Repeati661 – 68525LRR 11
Add
BLAST
Repeati686 – 71429LRR 12
Add
BLAST
Repeati715 – 74026LRR 13
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi38 – 4912Poly-Asn
Add
BLAST
Compositional biasi1045 – 112480Asn-rich
Add
BLAST

Sequence similaritiesi

Contains 1 F-box domain.

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiNOG300245.
GeneTreeiENSGT00750000117590.
HOGENOMiHOG000112822.
KOiK03360.
OMAiGHCFNIT.
OrthoDBiEOG7V76FS.

Family and domain databases

InterProiIPR001810. F-box_dom.
IPR026940. GrrA/Grr1.
[Graphical view]
PANTHERiPTHR23125:SF260. PTHR23125:SF260. 1 hit.
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24814-1 [UniParc]FASTAAdd to Basket

« Hide

MDQDNNNHND SNRLHPPDIH PNLGPQLWLN SSGDFDDNNN NNNNNNNNNS     50
TRPQMPSRTR ETATSERNAS EVRDATLNNI FRFDSIQRET LLPTNNGQPL 100
NQNFSLTFQP QQQTNALNGI DINTVNTNLM NGVNVQIDQL NRLLPNLPEE 150
ERKQIHEFKL IVGKKIQEFL VVIEKRRKKI LNEIELDNLK LKELRIDNSP 200
QAISYLHKLQ RMRLRALETE NMEIRNLRLK ILTIIEEYKK SLYAYCHSKL 250
RGQQVENPTD NFIIWINSID TTESSDLKEG LQDLSRYSRQ FINNVLSNPS 300
NQNICTSVTR RSPVFALNML PSEILHLILD KLNQKYDIVK FLTVSKLWAE 350
IIVKILYYRP HINKKSQLDL FLRTMKLTSE ETVFNYRLMI KRLNFSFVGD 400
YMHDTELNYF VGCKNLERLT LVFCKHITSV PISAVLRGCK FLQSVDITGI 450
RDVSDDVFDT LATYCPRVQG FYVPQARNVT FDSLRNFIVH SPMLKRIKIT 500
ANNNMNDELV ELLANKCPLL VEVDITLSPN VTDSSLLKLL TRLVQLREFR 550
ITHNTNITDN LFQELSKVVD DMPSLRLIDL SGCENITDKT IESIVNLAPK 600
LRNVFLGKCS RITDASLFQL SKLGKNLQTV HFGHCFNITD NGVRALFHSC 650
TRIQYVDFAC CTNLTNRTLY ELADLPKLKR IGLVKCTQMT DEGLLNMVSL 700
RGRNDTLERV HLSYCSNLTI YPIYELLMSC PRLSHLSLTA VPSFLRPDIT 750
MYCRPAPSDF SENQRQIFCV FSGKGVHKLR HYLVNLTSPA FGPHVDVNDV 800
LTKYIRSKNL IFNGETLEDA LRRIITDLNQ DSAAIIAATG LNQINGLNND 850
FLFQNINFER IDEVFSWYLN TFDGIRMSSE EVNSLLLQVN KTFCEDPFSD 900
VDDDQDYVVA PGVNREINSE MCHIVRKFHE LNDHIDDFEV NVASLVRVQF 950
QFTGFLLHEM TQTYMQMIEL NRQICLVQKT VQESGNIDYQ KGLLIWRLLF 1000
IDKFIMVVQK YKLSTVVLRL YLKDNITLLT RQRELLIAHQ RSAWNNNNDN 1050
DANRNANNIV NIVSDAGAND TSNNETNNGN DDNETENPNF WRQFGNRMQI 1100
SPDQMRNLQM GLRNQNMVRN NNNNTIDESM PDTAIDSQMD EASGTPDEDM 1150
L 1151
Length:1,151
Mass (Da):132,734
Last modified:March 1, 1992 - v1
Checksum:i6BB6C46611E6F825
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59247 Genomic DNA. Translation: AAA34652.1.
Z49590 Genomic DNA. Translation: CAA89617.1.
L47993 Genomic DNA. Translation: AAB39313.1.
BK006943 Genomic DNA. Translation: DAA08874.1.
PIRiA41529.
RefSeqiNP_012623.1. NM_001181747.1.

Genome annotation databases

EnsemblFungiiYJR090C; YJR090C; YJR090C.
GeneIDi853552.
KEGGisce:YJR090C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59247 Genomic DNA. Translation: AAA34652.1 .
Z49590 Genomic DNA. Translation: CAA89617.1 .
L47993 Genomic DNA. Translation: AAB39313.1 .
BK006943 Genomic DNA. Translation: DAA08874.1 .
PIRi A41529.
RefSeqi NP_012623.1. NM_001181747.1.

3D structure databases

ProteinModelPortali P24814.
SMRi P24814. Positions 483-753.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33844. 90 interactions.
DIPi DIP-1626N.
IntActi P24814. 8 interactions.
MINTi MINT-396112.
STRINGi 4932.YJR090C.

Proteomic databases

MaxQBi P24814.
PaxDbi P24814.
PeptideAtlasi P24814.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR090C ; YJR090C ; YJR090C .
GeneIDi 853552.
KEGGi sce:YJR090C.

Organism-specific databases

CYGDi YJR090c.
SGDi S000003850. GRR1.

Phylogenomic databases

eggNOGi NOG300245.
GeneTreei ENSGT00750000117590.
HOGENOMi HOG000112822.
KOi K03360.
OMAi GHCFNIT.
OrthoDBi EOG7V76FS.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci YEAST:G3O-31717-MONOMER.

Miscellaneous databases

NextBioi 974287.

Gene expression databases

Genevestigatori P24814.

Family and domain databases

InterProi IPR001810. F-box_dom.
IPR026940. GrrA/Grr1.
[Graphical view ]
PANTHERi PTHR23125:SF260. PTHR23125:SF260. 1 hit.
SMARTi SM00256. FBOX. 1 hit.
[Graphical view ]
PROSITEi PS50181. FBOX. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "GRR1 of Saccharomyces cerevisiae is required for glucose repression and encodes a protein with leucine-rich repeats."
    Flick J.S., Johnston M.
    Mol. Cell. Biol. 11:5101-5112(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, LEUCINE-RICH REPEATS.
  2. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
    Huang M.-E., Manus V., Chuat J.-C., Galibert F.
    Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "G1 cyclin turnover and nutrient uptake are controlled by a common pathway in yeast."
    Barral Y., Jentsch S., Mann C.
    Genes Dev. 9:399-409(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN UBIQUITINATION OF CLN1 AND CLN2.
  6. "The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex."
    Jaquenoud M., Gulli M.P., Peter K., Peter M.
    EMBO J. 17:5360-5373(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN UBIQUITINATION OF GIC2.
  7. "Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1."
    Skowyra D., Koepp D.M., Kamura T., Conrad M.N., Conaway R.C., Conaway J.W., Elledge S.J., Harper J.W.
    Science 284:662-665(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN SCF COMPLEX, FUNCTION IN UBIQUITINATION OF CLN1.
  8. "Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro."
    Kus B.M., Caldon C.E., Andorn-Broza R., Edwards A.M.
    Proteins 54:455-467(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKP1, RECONSTITUTION OF THE SCF(GRR1) COMPLEX.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-300, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGRR1_YEAST
AccessioniPrimary (citable) accession number: P24814
Secondary accession number(s): D6VWQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: July 9, 2014
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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