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P24814

- GRR1_YEAST

UniProt

P24814 - GRR1_YEAST

Protein

SCF E3 ubiquitin ligase complex F-box protein GRR1

Gene

GRR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and directs ubiquitination of phosphorylated CLN1, CLN2 and GIC2. Probably constitutes the primary response element required for the generation or interpretation of the signal that induces glucose repression.4 Publications

    Pathwayi

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein binding, bridging Source: SGD

    GO - Biological processi

    1. cell cycle arrest in response to pheromone Source: SGD
    2. cellular response to methylmercury Source: SGD
    3. G1/S transition of mitotic cell cycle Source: SGD
    4. glucose metabolic process Source: UniProtKB-KW
    5. protein polyubiquitination Source: SGD
    6. protein ubiquitination Source: SGD
    7. SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism, Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31717-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SCF E3 ubiquitin ligase complex F-box protein GRR1
    Alternative name(s):
    F-box and leucine-rich repeat protein GRR1
    F-box/LRR-repeat protein GRR1
    Gene namesi
    Name:GRR1
    Synonyms:CAT80, COT2
    Ordered Locus Names:YJR090C
    ORF Names:J1885
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR090c.
    SGDiS000003850. GRR1.

    Subcellular locationi

    Membrane 1 Publication
    Note: Associated with the particulate fraction. Probably forms a complex by protein-protein interactions via its leucine-rich segment.

    GO - Cellular componenti

    1. cellular bud neck contractile ring Source: SGD
    2. cytoplasm Source: SGD
    3. membrane Source: UniProtKB-SubCell
    4. nucleus Source: SGD
    5. SCF ubiquitin ligase complex Source: SGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11511151SCF E3 ubiquitin ligase complex F-box protein GRR1PRO_0000119968Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei199 – 1991Phosphoserine1 Publication
    Modified residuei300 – 3001Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP24814.
    PaxDbiP24814.
    PeptideAtlasiP24814.

    Expressioni

    Inductioni

    Expressed constitutively at low levels.

    Gene expression databases

    GenevestigatoriP24814.

    Interactioni

    Subunit structurei

    Interacts with SKP1. Component of the probable SCF(GRR1) complex containing CDC53, SKP1, RBX1 and GRR1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CDC53Q120188EBI-7898,EBI-4321
    SKP1P522866EBI-7898,EBI-4090

    Protein-protein interaction databases

    BioGridi33844. 90 interactions.
    DIPiDIP-1626N.
    IntActiP24814. 8 interactions.
    MINTiMINT-396112.
    STRINGi4932.YJR090C.

    Structurei

    3D structure databases

    ProteinModelPortaliP24814.
    SMRiP24814. Positions 483-753.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini314 – 36148F-boxPROSITE-ProRule annotationAdd
    BLAST
    Repeati399 – 42325LRR 11 PublicationAdd
    BLAST
    Repeati424 – 44926LRR 21 PublicationAdd
    BLAST
    Repeati450 – 47526LRR 31 PublicationAdd
    BLAST
    Repeati476 – 50126LRR 41 PublicationAdd
    BLAST
    Repeati502 – 52726LRR 51 PublicationAdd
    BLAST
    Repeati528 – 55326LRR 61 PublicationAdd
    BLAST
    Repeati554 – 58229LRR 71 PublicationAdd
    BLAST
    Repeati583 – 60826LRR 81 PublicationAdd
    BLAST
    Repeati609 – 63426LRR 91 PublicationAdd
    BLAST
    Repeati635 – 66026LRR 101 PublicationAdd
    BLAST
    Repeati661 – 68525LRR 111 PublicationAdd
    BLAST
    Repeati686 – 71429LRR 121 PublicationAdd
    BLAST
    Repeati715 – 74026LRR 131 PublicationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi38 – 4912Poly-AsnAdd
    BLAST
    Compositional biasi1045 – 112480Asn-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 F-box domain.PROSITE-ProRule annotation
    Contains 13 LRR (leucine-rich) repeats.Curated

    Keywords - Domaini

    Leucine-rich repeat, Repeat

    Phylogenomic databases

    eggNOGiNOG300245.
    GeneTreeiENSGT00750000117590.
    HOGENOMiHOG000112822.
    KOiK03360.
    OMAiGHCFNIT.
    OrthoDBiEOG7V76FS.

    Family and domain databases

    InterProiIPR001810. F-box_dom.
    IPR026940. GrrA/Grr1.
    [Graphical view]
    PANTHERiPTHR23125:SF260. PTHR23125:SF260. 1 hit.
    SMARTiSM00256. FBOX. 1 hit.
    [Graphical view]
    PROSITEiPS50181. FBOX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24814-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDQDNNNHND SNRLHPPDIH PNLGPQLWLN SSGDFDDNNN NNNNNNNNNS     50
    TRPQMPSRTR ETATSERNAS EVRDATLNNI FRFDSIQRET LLPTNNGQPL 100
    NQNFSLTFQP QQQTNALNGI DINTVNTNLM NGVNVQIDQL NRLLPNLPEE 150
    ERKQIHEFKL IVGKKIQEFL VVIEKRRKKI LNEIELDNLK LKELRIDNSP 200
    QAISYLHKLQ RMRLRALETE NMEIRNLRLK ILTIIEEYKK SLYAYCHSKL 250
    RGQQVENPTD NFIIWINSID TTESSDLKEG LQDLSRYSRQ FINNVLSNPS 300
    NQNICTSVTR RSPVFALNML PSEILHLILD KLNQKYDIVK FLTVSKLWAE 350
    IIVKILYYRP HINKKSQLDL FLRTMKLTSE ETVFNYRLMI KRLNFSFVGD 400
    YMHDTELNYF VGCKNLERLT LVFCKHITSV PISAVLRGCK FLQSVDITGI 450
    RDVSDDVFDT LATYCPRVQG FYVPQARNVT FDSLRNFIVH SPMLKRIKIT 500
    ANNNMNDELV ELLANKCPLL VEVDITLSPN VTDSSLLKLL TRLVQLREFR 550
    ITHNTNITDN LFQELSKVVD DMPSLRLIDL SGCENITDKT IESIVNLAPK 600
    LRNVFLGKCS RITDASLFQL SKLGKNLQTV HFGHCFNITD NGVRALFHSC 650
    TRIQYVDFAC CTNLTNRTLY ELADLPKLKR IGLVKCTQMT DEGLLNMVSL 700
    RGRNDTLERV HLSYCSNLTI YPIYELLMSC PRLSHLSLTA VPSFLRPDIT 750
    MYCRPAPSDF SENQRQIFCV FSGKGVHKLR HYLVNLTSPA FGPHVDVNDV 800
    LTKYIRSKNL IFNGETLEDA LRRIITDLNQ DSAAIIAATG LNQINGLNND 850
    FLFQNINFER IDEVFSWYLN TFDGIRMSSE EVNSLLLQVN KTFCEDPFSD 900
    VDDDQDYVVA PGVNREINSE MCHIVRKFHE LNDHIDDFEV NVASLVRVQF 950
    QFTGFLLHEM TQTYMQMIEL NRQICLVQKT VQESGNIDYQ KGLLIWRLLF 1000
    IDKFIMVVQK YKLSTVVLRL YLKDNITLLT RQRELLIAHQ RSAWNNNNDN 1050
    DANRNANNIV NIVSDAGAND TSNNETNNGN DDNETENPNF WRQFGNRMQI 1100
    SPDQMRNLQM GLRNQNMVRN NNNNTIDESM PDTAIDSQMD EASGTPDEDM 1150
    L 1151
    Length:1,151
    Mass (Da):132,734
    Last modified:March 1, 1992 - v1
    Checksum:i6BB6C46611E6F825
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59247 Genomic DNA. Translation: AAA34652.1.
    Z49590 Genomic DNA. Translation: CAA89617.1.
    L47993 Genomic DNA. Translation: AAB39313.1.
    BK006943 Genomic DNA. Translation: DAA08874.1.
    PIRiA41529.
    RefSeqiNP_012623.1. NM_001181747.1.

    Genome annotation databases

    EnsemblFungiiYJR090C; YJR090C; YJR090C.
    GeneIDi853552.
    KEGGisce:YJR090C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59247 Genomic DNA. Translation: AAA34652.1 .
    Z49590 Genomic DNA. Translation: CAA89617.1 .
    L47993 Genomic DNA. Translation: AAB39313.1 .
    BK006943 Genomic DNA. Translation: DAA08874.1 .
    PIRi A41529.
    RefSeqi NP_012623.1. NM_001181747.1.

    3D structure databases

    ProteinModelPortali P24814.
    SMRi P24814. Positions 483-753.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33844. 90 interactions.
    DIPi DIP-1626N.
    IntActi P24814. 8 interactions.
    MINTi MINT-396112.
    STRINGi 4932.YJR090C.

    Proteomic databases

    MaxQBi P24814.
    PaxDbi P24814.
    PeptideAtlasi P24814.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR090C ; YJR090C ; YJR090C .
    GeneIDi 853552.
    KEGGi sce:YJR090C.

    Organism-specific databases

    CYGDi YJR090c.
    SGDi S000003850. GRR1.

    Phylogenomic databases

    eggNOGi NOG300245.
    GeneTreei ENSGT00750000117590.
    HOGENOMi HOG000112822.
    KOi K03360.
    OMAi GHCFNIT.
    OrthoDBi EOG7V76FS.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci YEAST:G3O-31717-MONOMER.

    Miscellaneous databases

    NextBioi 974287.

    Gene expression databases

    Genevestigatori P24814.

    Family and domain databases

    InterProi IPR001810. F-box_dom.
    IPR026940. GrrA/Grr1.
    [Graphical view ]
    PANTHERi PTHR23125:SF260. PTHR23125:SF260. 1 hit.
    SMARTi SM00256. FBOX. 1 hit.
    [Graphical view ]
    PROSITEi PS50181. FBOX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "GRR1 of Saccharomyces cerevisiae is required for glucose repression and encodes a protein with leucine-rich repeats."
      Flick J.S., Johnston M.
      Mol. Cell. Biol. 11:5101-5112(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, LEUCINE-RICH REPEATS.
    2. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
      Huang M.-E., Manus V., Chuat J.-C., Galibert F.
      Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "G1 cyclin turnover and nutrient uptake are controlled by a common pathway in yeast."
      Barral Y., Jentsch S., Mann C.
      Genes Dev. 9:399-409(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN UBIQUITINATION OF CLN1 AND CLN2.
    6. "The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex."
      Jaquenoud M., Gulli M.P., Peter K., Peter M.
      EMBO J. 17:5360-5373(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN UBIQUITINATION OF GIC2.
    7. "Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1."
      Skowyra D., Koepp D.M., Kamura T., Conrad M.N., Conaway R.C., Conaway J.W., Elledge S.J., Harper J.W.
      Science 284:662-665(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN SCF COMPLEX, FUNCTION IN UBIQUITINATION OF CLN1.
    8. "Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro."
      Kus B.M., Caldon C.E., Andorn-Broza R., Edwards A.M.
      Proteins 54:455-467(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SKP1, RECONSTITUTION OF THE SCF(GRR1) COMPLEX.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-300, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGRR1_YEAST
    AccessioniPrimary (citable) accession number: P24814
    Secondary accession number(s): D6VWQ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 142 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3