P24814 (GRR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SCF E3 ubiquitin ligase complex F-box protein GRR1

Alternative name(s):
F-box and leucine-rich repeat protein GRR1
F-box/LRR-repeat protein GRR1

Gene names

Name:	GRR1
Synonyms:	CAT80, COT2
Ordered Locus Names:	YJR090C
ORF Names:	J1885

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1151 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and directs ubiquitination of phosphorylated CLN1, CLN2 and GIC2. Probably constitutes the primary response element required for the generation or interpretation of the signal that induces glucose repression. Ref.1 Ref.5 Ref.6 Ref.7
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SKP1. Component of the probable SCF(GRR1) complex containing CDC53, SKP1, RBX1 and GRR1. Ref.8
Subcellular location	Membrane. Note: Associated with the particulate fraction. Probably forms a complex by protein-protein interactions via its leucine-rich segment. Ref.1
Induction	Expressed constitutively at low levels.
Sequence similarities	Contains 1 F-box domain. Contains 13 LRR (leucine-rich) repeats.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism Ubl conjugation pathway
Cellular component	Membrane
Domain	Leucine-rich repeat Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G1/S transition of mitotic cell cycle Traceable author statement. Source: SGD SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from physical interaction Ref.7. Source: SGD cell cycle arrest in response to pheromone Inferred from mutant phenotype. Source: SGD cellular response to methylmercury Inferred from mutant phenotype. Source: SGD glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination Inferred from direct assay Ref.7. Source: GOC response to DNA damage stimulus Inferred from mutant phenotype. Source: SGD
Cellular component	SCF ubiquitin ligase complex Inferred from mutant phenotype Ref.6. Source: SGD cellular bud neck contractile ring Inferred from direct assay. Source: SGD membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: SGD
Molecular function	protein binding, bridging Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC53	Q12018	8	EBI-7898,EBI-4321
SKP1	P52286	8	EBI-7898,EBI-4090

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1151	1151	SCF E3 ubiquitin ligase complex F-box protein GRR1		PRO_0000119968
Regions
	Domain	314 – 361	48	F-box
	Repeat	399 – 423	25	LRR 1
	Repeat	424 – 449	26	LRR 2
	Repeat	450 – 475	26	LRR 3
	Repeat	476 – 501	26	LRR 4
	Repeat	502 – 527	26	LRR 5
	Repeat	528 – 553	26	LRR 6
	Repeat	554 – 582	29	LRR 7
	Repeat	583 – 608	26	LRR 8
	Repeat	609 – 634	26	LRR 9
	Repeat	635 – 660	26	LRR 10
	Repeat	661 – 685	25	LRR 11
	Repeat	686 – 714	29	LRR 12
	Repeat	715 – 740	26	LRR 13
	Compositional bias	38 – 49	12	Poly-Asn
	Compositional bias	1045 – 1124	80	Asn-rich
Amino acid modifications
	Modified residue	199	1	Phosphoserine Ref.9 Ref.10
	Modified residue	300	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P24814 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 6BB6C46611E6F825	FASTA	1,151	132,734
	10 20 30 40 50 60 MDQDNNNHND SNRLHPPDIH PNLGPQLWLN SSGDFDDNNN NNNNNNNNNS TRPQMPSRTR 70 80 90 100 110 120 ETATSERNAS EVRDATLNNI FRFDSIQRET LLPTNNGQPL NQNFSLTFQP QQQTNALNGI 130 140 150 160 170 180 DINTVNTNLM NGVNVQIDQL NRLLPNLPEE ERKQIHEFKL IVGKKIQEFL VVIEKRRKKI 190 200 210 220 230 240 LNEIELDNLK LKELRIDNSP QAISYLHKLQ RMRLRALETE NMEIRNLRLK ILTIIEEYKK 250 260 270 280 290 300 SLYAYCHSKL RGQQVENPTD NFIIWINSID TTESSDLKEG LQDLSRYSRQ FINNVLSNPS 310 320 330 340 350 360 NQNICTSVTR RSPVFALNML PSEILHLILD KLNQKYDIVK FLTVSKLWAE IIVKILYYRP 370 380 390 400 410 420 HINKKSQLDL FLRTMKLTSE ETVFNYRLMI KRLNFSFVGD YMHDTELNYF VGCKNLERLT 430 440 450 460 470 480 LVFCKHITSV PISAVLRGCK FLQSVDITGI RDVSDDVFDT LATYCPRVQG FYVPQARNVT 490 500 510 520 530 540 FDSLRNFIVH SPMLKRIKIT ANNNMNDELV ELLANKCPLL VEVDITLSPN VTDSSLLKLL 550 560 570 580 590 600 TRLVQLREFR ITHNTNITDN LFQELSKVVD DMPSLRLIDL SGCENITDKT IESIVNLAPK 610 620 630 640 650 660 LRNVFLGKCS RITDASLFQL SKLGKNLQTV HFGHCFNITD NGVRALFHSC TRIQYVDFAC 670 680 690 700 710 720 CTNLTNRTLY ELADLPKLKR IGLVKCTQMT DEGLLNMVSL RGRNDTLERV HLSYCSNLTI 730 740 750 760 770 780 YPIYELLMSC PRLSHLSLTA VPSFLRPDIT MYCRPAPSDF SENQRQIFCV FSGKGVHKLR 790 800 810 820 830 840 HYLVNLTSPA FGPHVDVNDV LTKYIRSKNL IFNGETLEDA LRRIITDLNQ DSAAIIAATG 850 860 870 880 890 900 LNQINGLNND FLFQNINFER IDEVFSWYLN TFDGIRMSSE EVNSLLLQVN KTFCEDPFSD 910 920 930 940 950 960 VDDDQDYVVA PGVNREINSE MCHIVRKFHE LNDHIDDFEV NVASLVRVQF QFTGFLLHEM 970 980 990 1000 1010 1020 TQTYMQMIEL NRQICLVQKT VQESGNIDYQ KGLLIWRLLF IDKFIMVVQK YKLSTVVLRL 1030 1040 1050 1060 1070 1080 YLKDNITLLT RQRELLIAHQ RSAWNNNNDN DANRNANNIV NIVSDAGAND TSNNETNNGN 1090 1100 1110 1120 1130 1140 DDNETENPNF WRQFGNRMQI SPDQMRNLQM GLRNQNMVRN NNNNTIDESM PDTAIDSQMD 1150 EASGTPDEDM L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GRR1 of Saccharomyces cerevisiae is required for glucose repression and encodes a protein with leucine-rich repeats." Flick J.S., Johnston M. Mol. Cell. Biol. 11:5101-5112(1991) [PubMed: 1922034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, LEUCINE-RICH REPEATS.
[2]	"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI." Huang M.-E., Manus V., Chuat J.-C., Galibert F. Yeast 12:869-875(1996) [PubMed: 8840504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"G1 cyclin turnover and nutrient uptake are controlled by a common pathway in yeast." Barral Y., Jentsch S., Mann C. Genes Dev. 9:399-409(1995) [PubMed: 7883165] [Abstract] Cited for: FUNCTION IN UBIQUITINATION OF CLN1 AND CLN2.
[6]	"The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex." Jaquenoud M., Gulli M.P., Peter K., Peter M. EMBO J. 17:5360-5373(1998) [PubMed: 9736614] [Abstract] Cited for: FUNCTION IN UBIQUITINATION OF GIC2.
[7]	"Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1." Skowyra D., Koepp D.M., Kamura T., Conrad M.N., Conaway R.C., Conaway J.W., Elledge S.J., Harper J.W. Science 284:662-665(1999) [PubMed: 10213692] [Abstract] Cited for: IDENTIFICATION IN SCF COMPLEX, FUNCTION IN UBIQUITINATION OF CLN1.
[8]	"Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro." Kus B.M., Caldon C.E., Andorn-Broza R., Edwards A.M. Proteins 54:455-467(2004) [PubMed: 14747994] [Abstract] Cited for: INTERACTION WITH SKP1, RECONSTITUTION OF THE SCF(GRR1) COMPLEX.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-300, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M59247 Genomic DNA. Translation: AAA34652.1. Z49590 Genomic DNA. Translation: CAA89617.1. L47993 Genomic DNA. Translation: AAB39313.1. BK006943 Genomic DNA. Translation: DAA08874.1.
PIR	A41529.
RefSeq	NP_012623.1. NM_001181747.1.
3D structure databases
ProteinModelPortal	P24814.
SMR	P24814. Positions 316-358, 384-477, 493-740.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1626N.
IntAct	P24814. 18 interactions.
MINT	MINT-396112.
STRING	P24814.
Proteomic databases
PeptideAtlas	P24814.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR090C; YJR090C; YJR090C.
GeneID	853552.
KEGG	sce:YJR090C.
NMPDR	fig|4932.3.peg.3597.
Organism-specific databases
CYGD	YJR090c.
SGD	S000003850. GRR1.
Phylogenomic databases
eggNOG	fuNOG05722.
GeneTree	EFGT00050000006075.
HOGENOM	HBG202254.
OMA	GHCFNIT.
OrthoDB	EOG4FBN21.
Gene expression databases
ArrayExpress	P24814.
Genevestigator	P24814.
GermOnline	YJR090C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001810. F-box_dom_cyclin-like. [Graphical view]
KO	K03360.
SMART	SM00256. FBOX. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974287.

Entry information

Entry name

GRR1_YEAST

Accession

Primary (citable) accession number: P24814
Secondary accession number(s): D6VWQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	January 25, 2012
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program