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Protein

SCF E3 ubiquitin ligase complex F-box protein GRR1

Gene

GRR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and directs ubiquitination of phosphorylated CLN1, CLN2 and GIC2. Probably constitutes the primary response element required for the generation or interpretation of the signal that induces glucose repression.4 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • protein binding, bridging Source: SGD

GO - Biological processi

  • cellular response to methylmercury Source: SGD
  • G1/S transition of mitotic cell cycle Source: SGD
  • glucose metabolic process Source: UniProtKB-KW
  • mitotic cell cycle arrest in response to pheromone Source: SGD
  • protein polyubiquitination Source: SGD
  • protein ubiquitination Source: SGD
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-31717-MONOMER.
ReactomeiR-SCE-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
SCF E3 ubiquitin ligase complex F-box protein GRR1
Alternative name(s):
F-box and leucine-rich repeat protein GRR1
F-box/LRR-repeat protein GRR1
Gene namesi
Name:GRR1
Synonyms:CAT80, COT2
Ordered Locus Names:YJR090C
ORF Names:J1885
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR090C.
SGDiS000003850. GRR1.

Subcellular locationi

  • Membrane 1 Publication

  • Note: Associated with the particulate fraction. Probably forms a complex by protein-protein interactions via its leucine-rich segment.

GO - Cellular componenti

  • cellular bud neck contractile ring Source: SGD
  • cytoplasm Source: SGD
  • membrane Source: UniProtKB-SubCell
  • nucleus Source: SGD
  • SCF ubiquitin ligase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001199681 – 1151SCF E3 ubiquitin ligase complex F-box protein GRR1Add BLAST1151

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei199PhosphoserineCombined sources1
Modified residuei300PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP24814.
PRIDEiP24814.

PTM databases

iPTMnetiP24814.

Expressioni

Inductioni

Expressed constitutively at low levels.

Interactioni

Subunit structurei

Interacts with SKP1. Component of the probable SCF(GRR1) complex containing CDC53, SKP1, RBX1 and GRR1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC53Q120188EBI-7898,EBI-4321
SKP1P522866EBI-7898,EBI-4090

GO - Molecular functioni

  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi33844. 95 interactors.
DIPiDIP-1626N.
IntActiP24814. 8 interactors.
MINTiMINT-396112.

Structurei

3D structure databases

ProteinModelPortaliP24814.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini314 – 361F-boxPROSITE-ProRule annotationAdd BLAST48
Repeati399 – 423LRR 11 PublicationAdd BLAST25
Repeati424 – 449LRR 21 PublicationAdd BLAST26
Repeati450 – 475LRR 31 PublicationAdd BLAST26
Repeati476 – 501LRR 41 PublicationAdd BLAST26
Repeati502 – 527LRR 51 PublicationAdd BLAST26
Repeati528 – 553LRR 61 PublicationAdd BLAST26
Repeati554 – 582LRR 71 PublicationAdd BLAST29
Repeati583 – 608LRR 81 PublicationAdd BLAST26
Repeati609 – 634LRR 91 PublicationAdd BLAST26
Repeati635 – 660LRR 101 PublicationAdd BLAST26
Repeati661 – 685LRR 111 PublicationAdd BLAST25
Repeati686 – 714LRR 121 PublicationAdd BLAST29
Repeati715 – 740LRR 131 PublicationAdd BLAST26

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi38 – 49Poly-AsnAdd BLAST12
Compositional biasi1045 – 1124Asn-richAdd BLAST80

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 13 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

GeneTreeiENSGT00760000119059.
HOGENOMiHOG000112822.
InParanoidiP24814.
KOiK03360.
OMAiVDFACCT.
OrthoDBiEOG092C0GWR.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR001810. F-box_dom.
IPR026940. GrrA/Grr1.
IPR032675. L_dom-like.
IPR006553. Leu-rich_rpt_Cys-con_subtyp.
[Graphical view]
PANTHERiPTHR24006:SF518. PTHR24006:SF518. 3 hits.
SMARTiSM00256. FBOX. 1 hit.
SM00367. LRR_CC. 11 hits.
[Graphical view]
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24814-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQDNNNHND SNRLHPPDIH PNLGPQLWLN SSGDFDDNNN NNNNNNNNNS
60 70 80 90 100
TRPQMPSRTR ETATSERNAS EVRDATLNNI FRFDSIQRET LLPTNNGQPL
110 120 130 140 150
NQNFSLTFQP QQQTNALNGI DINTVNTNLM NGVNVQIDQL NRLLPNLPEE
160 170 180 190 200
ERKQIHEFKL IVGKKIQEFL VVIEKRRKKI LNEIELDNLK LKELRIDNSP
210 220 230 240 250
QAISYLHKLQ RMRLRALETE NMEIRNLRLK ILTIIEEYKK SLYAYCHSKL
260 270 280 290 300
RGQQVENPTD NFIIWINSID TTESSDLKEG LQDLSRYSRQ FINNVLSNPS
310 320 330 340 350
NQNICTSVTR RSPVFALNML PSEILHLILD KLNQKYDIVK FLTVSKLWAE
360 370 380 390 400
IIVKILYYRP HINKKSQLDL FLRTMKLTSE ETVFNYRLMI KRLNFSFVGD
410 420 430 440 450
YMHDTELNYF VGCKNLERLT LVFCKHITSV PISAVLRGCK FLQSVDITGI
460 470 480 490 500
RDVSDDVFDT LATYCPRVQG FYVPQARNVT FDSLRNFIVH SPMLKRIKIT
510 520 530 540 550
ANNNMNDELV ELLANKCPLL VEVDITLSPN VTDSSLLKLL TRLVQLREFR
560 570 580 590 600
ITHNTNITDN LFQELSKVVD DMPSLRLIDL SGCENITDKT IESIVNLAPK
610 620 630 640 650
LRNVFLGKCS RITDASLFQL SKLGKNLQTV HFGHCFNITD NGVRALFHSC
660 670 680 690 700
TRIQYVDFAC CTNLTNRTLY ELADLPKLKR IGLVKCTQMT DEGLLNMVSL
710 720 730 740 750
RGRNDTLERV HLSYCSNLTI YPIYELLMSC PRLSHLSLTA VPSFLRPDIT
760 770 780 790 800
MYCRPAPSDF SENQRQIFCV FSGKGVHKLR HYLVNLTSPA FGPHVDVNDV
810 820 830 840 850
LTKYIRSKNL IFNGETLEDA LRRIITDLNQ DSAAIIAATG LNQINGLNND
860 870 880 890 900
FLFQNINFER IDEVFSWYLN TFDGIRMSSE EVNSLLLQVN KTFCEDPFSD
910 920 930 940 950
VDDDQDYVVA PGVNREINSE MCHIVRKFHE LNDHIDDFEV NVASLVRVQF
960 970 980 990 1000
QFTGFLLHEM TQTYMQMIEL NRQICLVQKT VQESGNIDYQ KGLLIWRLLF
1010 1020 1030 1040 1050
IDKFIMVVQK YKLSTVVLRL YLKDNITLLT RQRELLIAHQ RSAWNNNNDN
1060 1070 1080 1090 1100
DANRNANNIV NIVSDAGAND TSNNETNNGN DDNETENPNF WRQFGNRMQI
1110 1120 1130 1140 1150
SPDQMRNLQM GLRNQNMVRN NNNNTIDESM PDTAIDSQMD EASGTPDEDM

L
Length:1,151
Mass (Da):132,734
Last modified:March 1, 1992 - v1
Checksum:i6BB6C46611E6F825
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59247 Genomic DNA. Translation: AAA34652.1.
Z49590 Genomic DNA. Translation: CAA89617.1.
L47993 Genomic DNA. Translation: AAB39313.1.
BK006943 Genomic DNA. Translation: DAA08874.1.
PIRiA41529.
RefSeqiNP_012623.1. NM_001181747.1.

Genome annotation databases

EnsemblFungiiYJR090C; YJR090C; YJR090C.
GeneIDi853552.
KEGGisce:YJR090C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59247 Genomic DNA. Translation: AAA34652.1.
Z49590 Genomic DNA. Translation: CAA89617.1.
L47993 Genomic DNA. Translation: AAB39313.1.
BK006943 Genomic DNA. Translation: DAA08874.1.
PIRiA41529.
RefSeqiNP_012623.1. NM_001181747.1.

3D structure databases

ProteinModelPortaliP24814.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33844. 95 interactors.
DIPiDIP-1626N.
IntActiP24814. 8 interactors.
MINTiMINT-396112.

PTM databases

iPTMnetiP24814.

Proteomic databases

MaxQBiP24814.
PRIDEiP24814.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR090C; YJR090C; YJR090C.
GeneIDi853552.
KEGGisce:YJR090C.

Organism-specific databases

EuPathDBiFungiDB:YJR090C.
SGDiS000003850. GRR1.

Phylogenomic databases

GeneTreeiENSGT00760000119059.
HOGENOMiHOG000112822.
InParanoidiP24814.
KOiK03360.
OMAiVDFACCT.
OrthoDBiEOG092C0GWR.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-31717-MONOMER.
ReactomeiR-SCE-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiP24814.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR001810. F-box_dom.
IPR026940. GrrA/Grr1.
IPR032675. L_dom-like.
IPR006553. Leu-rich_rpt_Cys-con_subtyp.
[Graphical view]
PANTHERiPTHR24006:SF518. PTHR24006:SF518. 3 hits.
SMARTiSM00256. FBOX. 1 hit.
SM00367. LRR_CC. 11 hits.
[Graphical view]
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRR1_YEAST
AccessioniPrimary (citable) accession number: P24814
Secondary accession number(s): D6VWQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 30, 2016
This is version 165 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.