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Protein

AP-1-like transcription activator YAP2

Gene

CAD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription activator closely related to YAP1. Like YAP1, its activity can be induced by cadmium and peroxide stress, probably involving similar mechanisms inducing nuclear accumulation and thus transcription activation. YAP2, like YAP1, preferentially binds to promoters with the core binding site 5'-TTA[CG]TAA-3', but does not activate transcription of genes known to be activated by YAP1 in response to oxidative stress (such as TRX2, SSA1, GSH1). It is still not clear whether it is involved in oxidative stress response. When overexpressed, it confers pleiotropic drug-resistance and increases cellular tolerance to cadmium, iron chelators and zinc.4 Publications

Miscellaneous

One of 8 closely related fungi-specific YAP proteins (YAP1 to YAP8), which all seem to be transcription activators of the environmental stress response and metabolism control pathways and to have similar but not identical DNA binding specificities.
Present with 623 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular response to cadmium ion Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to stress Source: SGD

Keywordsi

Molecular functionActivator, DNA-binding
Biological processCadmium resistance, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29964-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1-like transcription activator YAP2
Alternative name(s):
Cadmium resistance protein 1
Transcription factor CAD1
Gene namesi
Name:CAD1
Synonyms:YAP2
Ordered Locus Names:YDR423C
ORF Names:D9461.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR423C.
SGDiS000002831. CAD1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000765221 – 409AP-1-like transcription activator YAP2Add BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi356 ↔ 387In nuclear retained form; alternate
Disulfide bondi356 ↔ 378In nuclear retained form; alternate
Disulfide bondi378 ↔ 387In nuclear retained form; alternate

Post-translational modificationi

Oxidative stress inducing agents may induce a conformational change involving disulfide bond formation between the conserved cysteine residues of the n-CRD (Cys-356, Cys-378 and Cys-387), masking as a result the nuclear export signal and thus causing nuclear accumulation.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP24813.
PRIDEiP24813.

Expressioni

Inductioni

YAP2 expression is at least partially regulated at the level of mRNA stability. Two small upstream open reading frames (uORF) in its mRNA cause increased RNA decay. The translation initiation factor eIF2 counteracts this effect by causing reinitiation at the functional initiation site, thus suppressing RNA decay.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi32482. 67 interactors.
DIPiDIP-4491N.
IntActiP24813. 4 interactors.
MINTiMINT-539300.
STRINGi4932.YDR423C.

Structurei

3D structure databases

ProteinModelPortaliP24813.
SMRiP24813.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 106bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni46 – 69Basic motifPROSITE-ProRule annotationAdd BLAST24
Regioni71 – 99Leucine-zipperPROSITE-ProRule annotationAdd BLAST29

Domaini

YAP2/CAD1 contains a C-terminal cysteine rich domain (c-CRD), but lacks the N-terminal CRD (n-CRD). It probably also contains embedded in the c-CRD a nuclear export signal, with which the nuclear export protein CRM1 may interact in the absence of disulfide bond formation within the c-CRD.

Sequence similaritiesi

Belongs to the bZIP family. YAP subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00530000069148.
InParanoidiP24813.
KOiK09043.
OrthoDBiEOG092C4KCZ.

Family and domain databases

Gene3Di1.10.238.100. 1 hit.
InterProiView protein in InterPro
IPR004827. bZIP.
IPR013910. TF_PAP1.
IPR023167. Yap1_redox_dom.
PfamiView protein in Pfam
PF00170. bZIP_1. 1 hit.
PF08601. PAP1. 1 hit.
SMARTiView protein in SMART
SM00338. BRLZ. 1 hit.
SUPFAMiSSF111430. SSF111430. 1 hit.
PROSITEiView protein in PROSITE
PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.

Sequencei

Sequence statusi: Complete.

P24813-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNILRKGQQ IYLAGDMKKQ MLLNKDGTPK RKVGRPGRKR IDSEAKSRRT
60 70 80 90 100
AQNRAAQRAF RDRKEAKMKS LQERVELLEQ KDAQNKTTTD FLLCSLKSLL
110 120 130 140 150
SEITKYRAKN SDDERILAFL DDLQEQQKRE NEKGTSTAVS KAAKELPSPN
160 170 180 190 200
SDENMTVNTS IEVQPHTQEN EKVMWNIGSW NAPSLTNSWD SPPGNRTGAV
210 220 230 240 250
TIGDESINGS EMPDFSLDLV SNDRQTGLEA LDYDIHNYFP QHSERLTAEK
260 270 280 290 300
IDTSACQCEI DQKYLPYETE DDTLFPSVLP LAVGSQCNNI CNRKCIGTKP
310 320 330 340 350
CSNKEIKCDL ITSHLLNQKS LASVLPVAAS HTKTIRTQSE AIEHISSAIS
360 370 380 390 400
NGKASCYHIL EEISSLPKYS SLDIDDLCSE LIIKAKCTDD CKIVVKARDL

QSALVRQLL
Length:409
Mass (Da):45,752
Last modified:October 1, 1993 - v2
Checksum:i21133AAAF40B0ED7
GO

Sequence cautioni

The sequence AAA34463 differs from that shown. Reason: Frameshift at positions 286 and 339.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36P → L in AAA34463 (PubMed:1514324).Curated1
Sequence conflicti90D → N in AAA34463 (PubMed:1514324).Curated1
Sequence conflicti102E → K in AAA34463 (PubMed:1514324).Curated1
Sequence conflicti125E → Q in AAA34463 (PubMed:1514324).Curated1
Sequence conflicti131N → D in AAA34463 (PubMed:1514324).Curated1
Sequence conflicti142A → V in AAA34463 (PubMed:1514324).Curated1
Sequence conflicti145E → G in AAA34463 (PubMed:1514324).Curated1
Sequence conflicti327V → L in AAB29937 (PubMed:8107671).Curated1
Sequence conflicti337T → I in AAB29937 (PubMed:8107671).Curated1
Sequence conflicti347S → I in AAB29937 (PubMed:8107671).Curated1
Sequence conflicti354A → P in AAB29937 (PubMed:8107671).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14289 Unassigned DNA. Translation: AAA02920.1.
X69106 mRNA. Translation: CAA48858.1.
S68847 Genomic DNA. Translation: AAB29937.1.
U33007 Genomic DNA. Translation: AAB64878.1.
M58331 Genomic DNA. Translation: AAA34463.1. Frameshift.
BK006938 Genomic DNA. Translation: DAA12263.1.
PIRiA48890.
RefSeqiNP_010711.3. NM_001180731.3.

Genome annotation databases

EnsemblFungiiYDR423C; YDR423C; YDR423C.
GeneIDi852033.
KEGGisce:YDR423C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiYAP2_YEAST
AccessioniPrimary (citable) accession number: P24813
Secondary accession number(s): D6VT53, Q02259
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 1, 1993
Last modified: July 5, 2017
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names