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P24808 (CWLA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-acetylmuramoyl-L-alanine amidase CwlA

EC=3.5.1.28
Alternative name(s):
Autolysin
Cell wall hydrolase
Gene names
Name:cwlA
Ordered Locus Names:BSU25900
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length272 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation.

Catalytic activity

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Subcellular location

Secreted Potential.

Sequence similarities

Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3939 By similarity
Chain40 – 272233N-acetylmuramoyl-L-alanine amidase CwlA
PRO_0000006453

Sequences

Sequence LengthMass (Da)Tools
P24808 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 43B2AB1ADC1F5DFA

FASTA27229,958
        10         20         30         40         50         60 
MAIKVVKNLV SKSKYGLKCP NPMKAEYITI HNTANDASAA NEISYMKNNS SSTSFHFAVD 

        70         80         90        100        110        120 
DKQVIQGIPT NRNAWHTGDG TNGTGNRKSI GVEICYSKSG GVRYKAAEKL AIKFVAQLLK 

       130        140        150        160        170        180 
ERGWGIDRVR KHQDWNGKYC PHRILSEGRW IQVKTAIEAE LKKLGGKTNS SKASVAKKKT 

       190        200        210        220        230        240 
TNTSSKKTSY ALPSGIFKVK SPMMRGEKVT QIQKALAALY FYPDKGAKNN GIDGVYGPKT 

       250        260        270 
ADAIRRFQSM YGLTQDGIYG PKTKAKLEAL LK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing and genetic mapping of a Bacillus subtilis cell wall hydrolase gene."
Kuroda A., Sekiguchi J.
J. Gen. Microbiol. 136:2209-2216(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21.
Strain: 168.
[2]"Cloning, expression, sequence analysis and biochemical characterization of an autolytic amidase of Bacillus subtilis 168 trpC2."
Foster S.J.
J. Gen. Microbiol. 137:1987-1998(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"Complete nucleotide sequence of a skin element excised by DNA rearrangement during sporulation in Bacillus subtilis."
Takemaru K., Mizuno M., Sato T., Takeuchi M., Kobayashi Y.
Microbiology 141:323-327(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[4]"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[5]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X51424 Genomic DNA. Translation: CAA35788.1.
M59232 Genomic DNA. Translation: AAA62676.1.
D32216 Genomic DNA. Translation: BAA06960.1.
D84432 Genomic DNA. Translation: BAA12424.1.
AL009126 Genomic DNA. Translation: CAB14531.1.
PIRC44816. S26671.
RefSeqNP_390467.1. NC_000964.3.

3D structure databases

ProteinModelPortalP24808.
SMRP24808. Positions 3-150, 198-270.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU25900.

Proteomic databases

PaxDbP24808.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14531; CAB14531; BSU25900.
GeneID937772.
KEGGbsu:BSU25900.
PATRIC18977024. VBIBacSub10457_2701.

Organism-specific databases

GenoListBSU25900. [Micado]

Phylogenomic databases

eggNOGCOG3409.
HOGENOMHOG000273688.
KOK01447.
OMAKAEYITI.
OrthoDBEOG67T5NN.
PhylomeDBP24808.

Enzyme and pathway databases

BioCycBSUB:BSU25900-MONOMER.

Family and domain databases

Gene3D1.10.101.10. 1 hit.
3.40.80.10. 1 hit.
InterProIPR002502. Amidase_domain.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamPF01510. Amidase_2. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
SMARTSM00644. Ami_2. 1 hit.
[Graphical view]
SUPFAMSSF47090. SSF47090. 1 hit.
SSF55846. SSF55846. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCWLA_BACSU
AccessionPrimary (citable) accession number: P24808
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList