Reviewed, UniProtKB/Swiss-Prot P24808 (CWLA_BACSU)

Names and origin

Protein names

Recommended name:
    N-acetylmuramoyl-L-alanine amidase cwlA
    EC=3.5.1.28
Alternative name(s):
    Cell wall hydrolase
    Autolysin

Gene names

Name:	cwlA
Ordered Locus Names:	BSU25900

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Competence Sporulation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW establishment of competence for transformation Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 39	39	By similarity
	Chain	40 – 272	233	N-acetylmuramoyl-L-alanine amidase cwlA		PRO_0000006453

Sequences

	Sequence		Length	Mass (Da)
	P24808-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 43B2AB1ADC1F5DFA	FASTA	272	29,958
	10 20 30 40 50 60 MAIKVVKNLV SKSKYGLKCP NPMKAEYITI HNTANDASAA NEISYMKNNS SSTSFHFAVD 70 80 90 100 110 120 DKQVIQGIPT NRNAWHTGDG TNGTGNRKSI GVEICYSKSG GVRYKAAEKL AIKFVAQLLK 130 140 150 160 170 180 ERGWGIDRVR KHQDWNGKYC PHRILSEGRW IQVKTAIEAE LKKLGGKTNS SKASVAKKKT 190 200 210 220 230 240 TNTSSKKTSY ALPSGIFKVK SPMMRGEKVT QIQKALAALY FYPDKGAKNN GIDGVYGPKT 250 260 270 ADAIRRFQSM YGLTQDGIYG PKTKAKLEAL LK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and genetic mapping of a Bacillus subtilis cell wall hydrolase gene." Kuroda A., Sekiguchi J. J. Gen. Microbiol. 136:2209-2216(1990) [PubMed: 2127796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21. Strain: 168.
[2]	"Cloning, expression, sequence analysis and biochemical characterization of an autolytic amidase of Bacillus subtilis 168 trpC2." Foster S.J. J. Gen. Microbiol. 137:1987-1998(1991) [PubMed: 1683402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"Complete nucleotide sequence of a skin element excised by DNA rearrangement during sporulation in Bacillus subtilis." Takemaru K., Mizuno M., Sato T., Takeuchi M., Kobayashi Y. Microbiology 141:323-327(1995) [PubMed: 7704261] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[4]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[5]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X51424 Genomic DNA. Translation: CAA35788.1. M59232 Genomic DNA. Translation: AAA62676.1. D32216 Genomic DNA. Translation: BAA06960.1. D84432 Genomic DNA. Translation: BAA12424.1. AL009126 Genomic DNA. Translation: CAB14531.1.
PIR	C44816. S26671.
RefSeq	NP_390467.1.
3D structure databases
HSSP	HSSP built from PDB template 1LBU based on UniProtKB P00733.
ModBase	Search...
Genome annotation databases
GeneID	937772.
GenomeReviews	Gene locus BSU25900 in contig AL009126_GR.
KEGG	bsu:BSU25900.
NMPDR	fig|224308.1.peg.2592.
Organism-specific databases
SubtiList	BG10505. cwlA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P24808.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2585-MON.
BRENDA	3.5.1.28. 150.
Family and domain databases
InterPro	IPR002502. Amidase_2. IPR002477. Peptidoglycan-bd-like. [Graphical view]
Gene3D	G3DSA:1.10.101.10. PGBD_1. 1 hit.
Pfam	PF01510. Amidase_2. 1 hit. PF01471. PG_binding_1. 1 hit. [Graphical view]
SMART	SM00644. Ami_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CWLA_BACSU

Accession

Primary (citable) accession number: P24808

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)