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P24808

- CWLA_BACSU

UniProt

P24808 - CWLA_BACSU

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Protein

N-acetylmuramoyl-L-alanine amidase CwlA

Gene
cwlA, BSU25900
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation.

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

GO - Molecular functioni

  1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

GO - Biological processi

  1. establishment of competence for transformation Source: UniProtKB-KW
  2. peptidoglycan catabolic process Source: InterPro
  3. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Competence, Sporulation

Enzyme and pathway databases

BioCyciBSUB:BSU25900-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylmuramoyl-L-alanine amidase CwlA (EC:3.5.1.28)
Alternative name(s):
Autolysin
Cell wall hydrolase
Gene namesi
Name:cwlA
Ordered Locus Names:BSU25900
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU25900. [Micado]

Subcellular locationi

Secreted Reviewed prediction

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3939 By similarityAdd
BLAST
Chaini40 – 272233N-acetylmuramoyl-L-alanine amidase CwlAPRO_0000006453Add
BLAST

Proteomic databases

PaxDbiP24808.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU25900.

Structurei

3D structure databases

ProteinModelPortaliP24808.
SMRiP24808. Positions 3-150, 198-270.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3409.
HOGENOMiHOG000273688.
KOiK01447.
OMAiKAEYITI.
OrthoDBiEOG67T5NN.
PhylomeDBiP24808.

Family and domain databases

Gene3Di1.10.101.10. 1 hit.
3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF01510. Amidase_2. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF55846. SSF55846. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24808-1 [UniParc]FASTAAdd to Basket

« Hide

MAIKVVKNLV SKSKYGLKCP NPMKAEYITI HNTANDASAA NEISYMKNNS    50
SSTSFHFAVD DKQVIQGIPT NRNAWHTGDG TNGTGNRKSI GVEICYSKSG 100
GVRYKAAEKL AIKFVAQLLK ERGWGIDRVR KHQDWNGKYC PHRILSEGRW 150
IQVKTAIEAE LKKLGGKTNS SKASVAKKKT TNTSSKKTSY ALPSGIFKVK 200
SPMMRGEKVT QIQKALAALY FYPDKGAKNN GIDGVYGPKT ADAIRRFQSM 250
YGLTQDGIYG PKTKAKLEAL LK 272
Length:272
Mass (Da):29,958
Last modified:March 1, 1992 - v1
Checksum:i43B2AB1ADC1F5DFA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51424 Genomic DNA. Translation: CAA35788.1.
M59232 Genomic DNA. Translation: AAA62676.1.
D32216 Genomic DNA. Translation: BAA06960.1.
D84432 Genomic DNA. Translation: BAA12424.1.
AL009126 Genomic DNA. Translation: CAB14531.1.
PIRiS26671. C44816.
RefSeqiNP_390467.1. NC_000964.3.
WP_003229946.1. NZ_CM000487.1.

Genome annotation databases

EnsemblBacteriaiCAB14531; CAB14531; BSU25900.
GeneIDi937772.
KEGGibsu:BSU25900.
PATRICi18977024. VBIBacSub10457_2701.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51424 Genomic DNA. Translation: CAA35788.1 .
M59232 Genomic DNA. Translation: AAA62676.1 .
D32216 Genomic DNA. Translation: BAA06960.1 .
D84432 Genomic DNA. Translation: BAA12424.1 .
AL009126 Genomic DNA. Translation: CAB14531.1 .
PIRi S26671. C44816.
RefSeqi NP_390467.1. NC_000964.3.
WP_003229946.1. NZ_CM000487.1.

3D structure databases

ProteinModelPortali P24808.
SMRi P24808. Positions 3-150, 198-270.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU25900.

Proteomic databases

PaxDbi P24808.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB14531 ; CAB14531 ; BSU25900 .
GeneIDi 937772.
KEGGi bsu:BSU25900.
PATRICi 18977024. VBIBacSub10457_2701.

Organism-specific databases

GenoListi BSU25900. [Micado ]

Phylogenomic databases

eggNOGi COG3409.
HOGENOMi HOG000273688.
KOi K01447.
OMAi KAEYITI.
OrthoDBi EOG67T5NN.
PhylomeDBi P24808.

Enzyme and pathway databases

BioCyci BSUB:BSU25900-MONOMER.

Family and domain databases

Gene3Di 1.10.101.10. 1 hit.
3.40.80.10. 1 hit.
InterProi IPR002502. Amidase_domain.
IPR002477. Peptidoglycan-bd-like.
[Graphical view ]
Pfami PF01510. Amidase_2. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view ]
SMARTi SM00644. Ami_2. 1 hit.
[Graphical view ]
SUPFAMi SSF47090. SSF47090. 1 hit.
SSF55846. SSF55846. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing and genetic mapping of a Bacillus subtilis cell wall hydrolase gene."
    Kuroda A., Sekiguchi J.
    J. Gen. Microbiol. 136:2209-2216(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21.
    Strain: 168.
  2. "Cloning, expression, sequence analysis and biochemical characterization of an autolytic amidase of Bacillus subtilis 168 trpC2."
    Foster S.J.
    J. Gen. Microbiol. 137:1987-1998(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "Complete nucleotide sequence of a skin element excised by DNA rearrangement during sporulation in Bacillus subtilis."
    Takemaru K., Mizuno M., Sato T., Takeuchi M., Kobayashi Y.
    Microbiology 141:323-327(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  4. "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
    Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
    Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  5. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiCWLA_BACSU
AccessioniPrimary (citable) accession number: P24808
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: September 3, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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