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P24808

- CWLA_BACSU

UniProt

P24808 - CWLA_BACSU

Protein

N-acetylmuramoyl-L-alanine amidase CwlA

Gene

cwlA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation.

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

    GO - Molecular functioni

    1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. establishment of competence for transformation Source: UniProtKB-KW
    2. peptidoglycan catabolic process Source: InterPro
    3. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation, Competence, Sporulation

    Enzyme and pathway databases

    BioCyciBSUB:BSU25900-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylmuramoyl-L-alanine amidase CwlA (EC:3.5.1.28)
    Alternative name(s):
    Autolysin
    Cell wall hydrolase
    Gene namesi
    Name:cwlA
    Ordered Locus Names:BSU25900
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU25900. [Micado]

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3939By similarityAdd
    BLAST
    Chaini40 – 272233N-acetylmuramoyl-L-alanine amidase CwlAPRO_0000006453Add
    BLAST

    Proteomic databases

    PaxDbiP24808.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU25900.

    Structurei

    3D structure databases

    ProteinModelPortaliP24808.
    SMRiP24808. Positions 3-150, 198-270.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3409.
    HOGENOMiHOG000273688.
    KOiK01447.
    OMAiKAEYITI.
    OrthoDBiEOG67T5NN.
    PhylomeDBiP24808.

    Family and domain databases

    Gene3Di1.10.101.10. 1 hit.
    3.40.80.10. 1 hit.
    InterProiIPR002502. Amidase_domain.
    IPR002477. Peptidoglycan-bd-like.
    [Graphical view]
    PfamiPF01510. Amidase_2. 1 hit.
    PF01471. PG_binding_1. 1 hit.
    [Graphical view]
    SMARTiSM00644. Ami_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF47090. SSF47090. 1 hit.
    SSF55846. SSF55846. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24808-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAIKVVKNLV SKSKYGLKCP NPMKAEYITI HNTANDASAA NEISYMKNNS    50
    SSTSFHFAVD DKQVIQGIPT NRNAWHTGDG TNGTGNRKSI GVEICYSKSG 100
    GVRYKAAEKL AIKFVAQLLK ERGWGIDRVR KHQDWNGKYC PHRILSEGRW 150
    IQVKTAIEAE LKKLGGKTNS SKASVAKKKT TNTSSKKTSY ALPSGIFKVK 200
    SPMMRGEKVT QIQKALAALY FYPDKGAKNN GIDGVYGPKT ADAIRRFQSM 250
    YGLTQDGIYG PKTKAKLEAL LK 272
    Length:272
    Mass (Da):29,958
    Last modified:March 1, 1992 - v1
    Checksum:i43B2AB1ADC1F5DFA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X51424 Genomic DNA. Translation: CAA35788.1.
    M59232 Genomic DNA. Translation: AAA62676.1.
    D32216 Genomic DNA. Translation: BAA06960.1.
    D84432 Genomic DNA. Translation: BAA12424.1.
    AL009126 Genomic DNA. Translation: CAB14531.1.
    PIRiS26671. C44816.
    RefSeqiNP_390467.1. NC_000964.3.
    WP_003229946.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB14531; CAB14531; BSU25900.
    GeneIDi937772.
    KEGGibsu:BSU25900.
    PATRICi18977024. VBIBacSub10457_2701.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X51424 Genomic DNA. Translation: CAA35788.1 .
    M59232 Genomic DNA. Translation: AAA62676.1 .
    D32216 Genomic DNA. Translation: BAA06960.1 .
    D84432 Genomic DNA. Translation: BAA12424.1 .
    AL009126 Genomic DNA. Translation: CAB14531.1 .
    PIRi S26671. C44816.
    RefSeqi NP_390467.1. NC_000964.3.
    WP_003229946.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali P24808.
    SMRi P24808. Positions 3-150, 198-270.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU25900.

    Proteomic databases

    PaxDbi P24808.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14531 ; CAB14531 ; BSU25900 .
    GeneIDi 937772.
    KEGGi bsu:BSU25900.
    PATRICi 18977024. VBIBacSub10457_2701.

    Organism-specific databases

    GenoListi BSU25900. [Micado ]

    Phylogenomic databases

    eggNOGi COG3409.
    HOGENOMi HOG000273688.
    KOi K01447.
    OMAi KAEYITI.
    OrthoDBi EOG67T5NN.
    PhylomeDBi P24808.

    Enzyme and pathway databases

    BioCyci BSUB:BSU25900-MONOMER.

    Family and domain databases

    Gene3Di 1.10.101.10. 1 hit.
    3.40.80.10. 1 hit.
    InterProi IPR002502. Amidase_domain.
    IPR002477. Peptidoglycan-bd-like.
    [Graphical view ]
    Pfami PF01510. Amidase_2. 1 hit.
    PF01471. PG_binding_1. 1 hit.
    [Graphical view ]
    SMARTi SM00644. Ami_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47090. SSF47090. 1 hit.
    SSF55846. SSF55846. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing and genetic mapping of a Bacillus subtilis cell wall hydrolase gene."
      Kuroda A., Sekiguchi J.
      J. Gen. Microbiol. 136:2209-2216(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21.
      Strain: 168.
    2. "Cloning, expression, sequence analysis and biochemical characterization of an autolytic amidase of Bacillus subtilis 168 trpC2."
      Foster S.J.
      J. Gen. Microbiol. 137:1987-1998(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "Complete nucleotide sequence of a skin element excised by DNA rearrangement during sporulation in Bacillus subtilis."
      Takemaru K., Mizuno M., Sato T., Takeuchi M., Kobayashi Y.
      Microbiology 141:323-327(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / JH642.
    4. "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
      Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
      Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / JH642.
    5. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.

    Entry informationi

    Entry nameiCWLA_BACSU
    AccessioniPrimary (citable) accession number: P24808
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3