Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P24806

- XTH24_ARATH

UniProt

P24806 - XTH24_ARATH

Protein

Xyloglucan endotransglucosylase/hydrolase protein 24

Gene

XTH24

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (11 Jul 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues. May be required during development to modify the walls of cells under mechanical stress.

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei98 – 981NucleophilePROSITE-ProRule annotation
    Active sitei102 – 1021Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: TAIR

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro
    2. plant-type cell wall loosening Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xyloglucan endotransglucosylase/hydrolase protein 24 (EC:2.4.1.207)
    Short name:
    At-XTH24
    Short name:
    XTH-24
    Alternative name(s):
    Endo-xyloglucan transferase
    Meristem protein 5
    Short name:
    MERI-5 protein
    Short name:
    MERI5 protein
    Xyloglucan endo-1,4-beta-D-glucanase
    Gene namesi
    Name:XTH24
    Synonyms:MERI-5, MERI5B, SEN4
    Ordered Locus Names:At4g30270
    ORF Names:F9N11.120
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G30270.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: TAIR
    3. cytoplasm Source: TAIR
    4. Golgi apparatus Source: TAIR
    5. plant-type cell wall Source: TAIR
    6. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 269248Xyloglucan endotransglucosylase/hydrolase protein 24PRO_0000011824Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi106 – 1061N-linked (GlcNAc...)1 Publication

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity
    N-glycosylated; essential for its enzymatic activity.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP24806.
    PRIDEiP24806.

    Expressioni

    Tissue specificityi

    Highly expressed. Predominantly expressed in stems. Expressed in shoot apical meristems, also found in seedlings and meristems.2 Publications

    Inductioni

    May be transcriptionally regulated by ANGUSTIFOLIA.1 Publication

    Gene expression databases

    ArrayExpressiP24806.
    GenevestigatoriP24806.

    Structurei

    3D structure databases

    ProteinModelPortaliP24806.
    SMRiP24806. Positions 46-256.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2273.
    HOGENOMiHOG000236368.
    InParanoidiP24806.
    KOiK08235.
    OMAiMASYRNI.
    PhylomeDBiP24806.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24806-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSPFKIFFFT TLLVAAFSVS AADFNTDVNV AWGNGRGKIL NNGQLLTLSL    50
    DKSSGSGFQS KTEYLFGKID MQIKLVPGNS AGTVTTFYLK SEGSTWDEID 100
    FEFLGNMSGD PYTLHTNVYT QGKGDKEQQF HLWFDPTANF HTYSILWNPQ 150
    RIILTVDDTP IREFKNYESL GVLFPKNKPM RMYASLWNAD DWATRGGLVK 200
    TDWSKAPFMA SYRNIKIDSK PNSNWYTQEM DSTSQARLKW VQKNYMIYNY 250
    CTDHRRFPQG APKECTTSS 269
    Length:269
    Mass (Da):30,756
    Last modified:July 11, 2002 - v2
    Checksum:i648F042BC7ADED86
    GO

    Sequence cautioni

    The sequence AAA32828.1 differs from that shown. Reason: Frameshift at positions 158, 178, 183, 189, 190, 194 and 199.
    The sequence CAA79012.1 differs from that shown. Reason: Frameshift at positions 93 and 104.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti65 – 651L → F in AAM63080. 1 PublicationCurated
    Sequence conflicti93 – 942GS → DR in AAC39467. (PubMed:9617812)Curated
    Sequence conflicti184 – 1841A → G(PubMed:1840916)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63166 Genomic DNA. Translation: AAA32828.1. Frameshift.
    D63508 mRNA. Translation: BAA09783.1.
    AL109796 Genomic DNA. Translation: CAB52471.1.
    AL161576 Genomic DNA. Translation: CAB81020.1.
    CP002687 Genomic DNA. Translation: AEE85745.1.
    AY035156 mRNA. Translation: AAK59660.1.
    AY063027 mRNA. Translation: AAL34201.1.
    AY085867 mRNA. Translation: AAM63080.1.
    Z17602 mRNA. Translation: CAA79012.1. Frameshift.
    AF035384 mRNA. Translation: AAC39467.1.
    X82683 Genomic DNA. Translation: CAA58001.1.
    PIRiS61555.
    T51754.
    RefSeqiNP_194756.1. NM_119173.3.
    UniGeneiAt.20967.
    At.26243.
    At.27681.
    At.47568.
    At.75103.

    Genome annotation databases

    EnsemblPlantsiAT4G30270.1; AT4G30270.1; AT4G30270.
    GeneIDi829150.
    KEGGiath:AT4G30270.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63166 Genomic DNA. Translation: AAA32828.1 . Frameshift.
    D63508 mRNA. Translation: BAA09783.1 .
    AL109796 Genomic DNA. Translation: CAB52471.1 .
    AL161576 Genomic DNA. Translation: CAB81020.1 .
    CP002687 Genomic DNA. Translation: AEE85745.1 .
    AY035156 mRNA. Translation: AAK59660.1 .
    AY063027 mRNA. Translation: AAL34201.1 .
    AY085867 mRNA. Translation: AAM63080.1 .
    Z17602 mRNA. Translation: CAA79012.1 . Frameshift.
    AF035384 mRNA. Translation: AAC39467.1 .
    X82683 Genomic DNA. Translation: CAA58001.1 .
    PIRi S61555.
    T51754.
    RefSeqi NP_194756.1. NM_119173.3.
    UniGenei At.20967.
    At.26243.
    At.27681.
    At.47568.
    At.75103.

    3D structure databases

    ProteinModelPortali P24806.
    SMRi P24806. Positions 46-256.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    PaxDbi P24806.
    PRIDEi P24806.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G30270.1 ; AT4G30270.1 ; AT4G30270 .
    GeneIDi 829150.
    KEGGi ath:AT4G30270.

    Organism-specific databases

    TAIRi AT4G30270.

    Phylogenomic databases

    eggNOGi COG2273.
    HOGENOMi HOG000236368.
    InParanoidi P24806.
    KOi K08235.
    OMAi MASYRNI.
    PhylomeDBi P24806.

    Gene expression databases

    ArrayExpressi P24806.
    Genevestigatori P24806.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of genes expressed in shoot apical meristems."
      Medford J.I., Elmer J.S., Klee H.J.
      Plant Cell 3:359-370(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    2. Kamimai T., Tomita E., Nishitani K.
      Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-132.
      Strain: cv. Columbia.
      Tissue: Green siliques.
    8. "Differential expression of senescence-associated mRNAs during leaf senescence induced by different senescence-inducing factors in Arabidopsis."
      Park J.-H., Oh S.A., Kim Y.H., Woo H.R., Nam H.G.
      Plant Mol. Biol. 37:445-454(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-120.
      Strain: cv. Columbia.
      Tissue: Leaf.
    9. "Characterisation of two tomato fruit-expressed cDNAs encoding xyloglucan endo-transglycosylase."
      Arrowsmith D.A., De Silva J.
      Plant Mol. Biol. 28:391-403(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 104-269.
      Strain: cv. Landsberg erecta.
    10. "In vitro activities of four xyloglucan endotransglycosylases from Arabidopsis."
      Campbell P., Braam J.
      Plant J. 18:371-382(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME ACTIVITY, GLYCOSYLATION.
    11. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
      Yokoyama R., Nishitani K.
      Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    12. "The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation."
      Kim G.-T., Shoda K., Tsuge T., Cho K.-H., Uchimiya H., Yokoyama R., Nishitani K., Tsukaya H.
      EMBO J. 21:1267-1279(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    13. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
      Rose J.K.C., Braam J., Fry S.C., Nishitani K.
      Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.

    Entry informationi

    Entry nameiXTH24_ARATH
    AccessioniPrimary (citable) accession number: P24806
    Secondary accession number(s): O64956
    , Q39148, Q39149, Q41904, Q8LDQ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: July 11, 2002
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3