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P24806

- XTH24_ARATH

UniProt

P24806 - XTH24_ARATH

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Protein
Xyloglucan endotransglucosylase/hydrolase protein 24
Gene
XTH24, MERI-5, MERI5B, SEN4, At4g30270, F9N11.120
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues. May be required during development to modify the walls of cells under mechanical stress.

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981Nucleophile By similarity
Active sitei102 – 1021Proton donor By similarity

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: TAIR

GO - Biological processi

  1. cellular glucan metabolic process Source: InterPro
  2. plant-type cell wall loosening Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglucosylase/hydrolase protein 24 (EC:2.4.1.207)
Short name:
At-XTH24
Short name:
XTH-24
Alternative name(s):
Endo-xyloglucan transferase
Meristem protein 5
Short name:
MERI-5 protein
Short name:
MERI5 protein
Xyloglucan endo-1,4-beta-D-glucanase
Gene namesi
Name:XTH24
Synonyms:MERI-5, MERI5B, SEN4
Ordered Locus Names:At4g30270
ORF Names:F9N11.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G30270.

Subcellular locationi

Secretedcell wall Inferred. Secretedextracellular spaceapoplast Inferred

GO - Cellular componenti

  1. Golgi apparatus Source: TAIR
  2. apoplast Source: UniProtKB-SubCell
  3. cell wall Source: TAIR
  4. cytoplasm Source: TAIR
  5. plant-type cell wall Source: TAIR
  6. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 269248Xyloglucan endotransglucosylase/hydrolase protein 24
PRO_0000011824Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi106 – 1061N-linked (GlcNAc...) Inferred

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.
N-glycosylated; essential for its enzymatic activity.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP24806.
PRIDEiP24806.

Expressioni

Tissue specificityi

Highly expressed. Predominantly expressed in stems. Expressed in shoot apical meristems, also found in seedlings and meristems.2 Publications

Inductioni

May be transcriptionally regulated by ANGUSTIFOLIA.1 Publication

Gene expression databases

ArrayExpressiP24806.
GenevestigatoriP24806.

Structurei

3D structure databases

ProteinModelPortaliP24806.
SMRiP24806. Positions 46-256.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiP24806.
KOiK08235.
OMAiMASYRNI.
PhylomeDBiP24806.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24806-1 [UniParc]FASTAAdd to Basket

« Hide

MSPFKIFFFT TLLVAAFSVS AADFNTDVNV AWGNGRGKIL NNGQLLTLSL    50
DKSSGSGFQS KTEYLFGKID MQIKLVPGNS AGTVTTFYLK SEGSTWDEID 100
FEFLGNMSGD PYTLHTNVYT QGKGDKEQQF HLWFDPTANF HTYSILWNPQ 150
RIILTVDDTP IREFKNYESL GVLFPKNKPM RMYASLWNAD DWATRGGLVK 200
TDWSKAPFMA SYRNIKIDSK PNSNWYTQEM DSTSQARLKW VQKNYMIYNY 250
CTDHRRFPQG APKECTTSS 269
Length:269
Mass (Da):30,756
Last modified:July 11, 2002 - v2
Checksum:i648F042BC7ADED86
GO

Sequence cautioni

The sequence AAA32828.1 differs from that shown. Reason: Frameshift at positions 158, 178, 183, 189, 190, 194 and 199.
The sequence CAA79012.1 differs from that shown. Reason: Frameshift at positions 93 and 104.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651L → F in AAM63080. 1 Publication
Sequence conflicti93 – 942GS → DR in AAC39467. 1 Publication
Sequence conflicti184 – 1841A → G1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63166 Genomic DNA. Translation: AAA32828.1. Frameshift.
D63508 mRNA. Translation: BAA09783.1.
AL109796 Genomic DNA. Translation: CAB52471.1.
AL161576 Genomic DNA. Translation: CAB81020.1.
CP002687 Genomic DNA. Translation: AEE85745.1.
AY035156 mRNA. Translation: AAK59660.1.
AY063027 mRNA. Translation: AAL34201.1.
AY085867 mRNA. Translation: AAM63080.1.
Z17602 mRNA. Translation: CAA79012.1. Frameshift.
AF035384 mRNA. Translation: AAC39467.1.
X82683 Genomic DNA. Translation: CAA58001.1.
PIRiS61555.
T51754.
RefSeqiNP_194756.1. NM_119173.3.
UniGeneiAt.20967.
At.26243.
At.27681.
At.47568.
At.75103.

Genome annotation databases

EnsemblPlantsiAT4G30270.1; AT4G30270.1; AT4G30270.
GeneIDi829150.
KEGGiath:AT4G30270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63166 Genomic DNA. Translation: AAA32828.1 . Frameshift.
D63508 mRNA. Translation: BAA09783.1 .
AL109796 Genomic DNA. Translation: CAB52471.1 .
AL161576 Genomic DNA. Translation: CAB81020.1 .
CP002687 Genomic DNA. Translation: AEE85745.1 .
AY035156 mRNA. Translation: AAK59660.1 .
AY063027 mRNA. Translation: AAL34201.1 .
AY085867 mRNA. Translation: AAM63080.1 .
Z17602 mRNA. Translation: CAA79012.1 . Frameshift.
AF035384 mRNA. Translation: AAC39467.1 .
X82683 Genomic DNA. Translation: CAA58001.1 .
PIRi S61555.
T51754.
RefSeqi NP_194756.1. NM_119173.3.
UniGenei At.20967.
At.26243.
At.27681.
At.47568.
At.75103.

3D structure databases

ProteinModelPortali P24806.
SMRi P24806. Positions 46-256.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbi P24806.
PRIDEi P24806.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G30270.1 ; AT4G30270.1 ; AT4G30270 .
GeneIDi 829150.
KEGGi ath:AT4G30270.

Organism-specific databases

TAIRi AT4G30270.

Phylogenomic databases

eggNOGi COG2273.
HOGENOMi HOG000236368.
InParanoidi P24806.
KOi K08235.
OMAi MASYRNI.
PhylomeDBi P24806.

Gene expression databases

ArrayExpressi P24806.
Genevestigatori P24806.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF005604. XET. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of genes expressed in shoot apical meristems."
    Medford J.I., Elmer J.S., Klee H.J.
    Plant Cell 3:359-370(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
  2. Kamimai T., Tomita E., Nishitani K.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-132.
    Strain: cv. Columbia.
    Tissue: Green siliques.
  8. "Differential expression of senescence-associated mRNAs during leaf senescence induced by different senescence-inducing factors in Arabidopsis."
    Park J.-H., Oh S.A., Kim Y.H., Woo H.R., Nam H.G.
    Plant Mol. Biol. 37:445-454(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-120.
    Strain: cv. Columbia.
    Tissue: Leaf.
  9. "Characterisation of two tomato fruit-expressed cDNAs encoding xyloglucan endo-transglycosylase."
    Arrowsmith D.A., De Silva J.
    Plant Mol. Biol. 28:391-403(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 104-269.
    Strain: cv. Landsberg erecta.
  10. "In vitro activities of four xyloglucan endotransglycosylases from Arabidopsis."
    Campbell P., Braam J.
    Plant J. 18:371-382(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME ACTIVITY, GLYCOSYLATION.
  11. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
    Yokoyama R., Nishitani K.
    Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  12. "The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation."
    Kim G.-T., Shoda K., Tsuge T., Cho K.-H., Uchimiya H., Yokoyama R., Nishitani K., Tsukaya H.
    EMBO J. 21:1267-1279(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  13. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
    Rose J.K.C., Braam J., Fry S.C., Nishitani K.
    Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiXTH24_ARATH
AccessioniPrimary (citable) accession number: P24806
Secondary accession number(s): O64956
, Q39148, Q39149, Q41904, Q8LDQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 11, 2002
Last modified: June 11, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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