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Protein

Xyloglucan endotransglucosylase/hydrolase protein 24

Gene

XTH24

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues. May be required during development to modify the walls of cells under mechanical stress.

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981NucleophilePROSITE-ProRule annotation
Active sitei102 – 1021Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: TAIR

GO - Biological processi

  1. cellular glucan metabolic process Source: InterPro
  2. plant-type cell wall loosening Source: TAIR
  3. plant-type cell wall organization or biogenesis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BRENDAi2.4.1.207. 399.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglucosylase/hydrolase protein 24 (EC:2.4.1.207)
Short name:
At-XTH24
Short name:
XTH-24
Alternative name(s):
Endo-xyloglucan transferase
Meristem protein 5
Short name:
MERI-5 protein
Short name:
MERI5 protein
Xyloglucan endo-1,4-beta-D-glucanase
Gene namesi
Name:XTH24
Synonyms:MERI-5, MERI5B, SEN4
Ordered Locus Names:At4g30270
ORF Names:F9N11.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G30270.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
  2. cell wall Source: TAIR
  3. cytoplasm Source: TAIR
  4. Golgi apparatus Source: TAIR
  5. plant-type cell wall Source: TAIR
  6. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 269248Xyloglucan endotransglucosylase/hydrolase protein 24PRO_0000011824Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi106 – 1061N-linked (GlcNAc...)1 Publication

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity
N-glycosylated; essential for its enzymatic activity.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP24806.
PRIDEiP24806.

Expressioni

Tissue specificityi

Highly expressed. Predominantly expressed in stems. Expressed in shoot apical meristems, also found in seedlings and meristems.2 Publications

Inductioni

May be transcriptionally regulated by ANGUSTIFOLIA.1 Publication

Gene expression databases

ExpressionAtlasiP24806. baseline and differential.
GenevestigatoriP24806.

Structurei

3D structure databases

ProteinModelPortaliP24806.
SMRiP24806. Positions 46-256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiP24806.
KOiK08235.
OMAiMASYRNI.
PhylomeDBiP24806.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24806-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPFKIFFFT TLLVAAFSVS AADFNTDVNV AWGNGRGKIL NNGQLLTLSL
60 70 80 90 100
DKSSGSGFQS KTEYLFGKID MQIKLVPGNS AGTVTTFYLK SEGSTWDEID
110 120 130 140 150
FEFLGNMSGD PYTLHTNVYT QGKGDKEQQF HLWFDPTANF HTYSILWNPQ
160 170 180 190 200
RIILTVDDTP IREFKNYESL GVLFPKNKPM RMYASLWNAD DWATRGGLVK
210 220 230 240 250
TDWSKAPFMA SYRNIKIDSK PNSNWYTQEM DSTSQARLKW VQKNYMIYNY
260
CTDHRRFPQG APKECTTSS
Length:269
Mass (Da):30,756
Last modified:July 10, 2002 - v2
Checksum:i648F042BC7ADED86
GO

Sequence cautioni

The sequence AAA32828.1 differs from that shown. Reason: Frameshift at positions 158, 178, 183, 189, 190, 194 and 199. Curated
The sequence CAA79012.1 differs from that shown. Reason: Frameshift at positions 93 and 104. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651L → F in AAM63080 (Ref. 6) Curated
Sequence conflicti93 – 942GS → DR in AAC39467 (PubMed:9617812).Curated
Sequence conflicti184 – 1841A → G (PubMed:1840916).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63166 Genomic DNA. Translation: AAA32828.1. Frameshift.
D63508 mRNA. Translation: BAA09783.1.
AL109796 Genomic DNA. Translation: CAB52471.1.
AL161576 Genomic DNA. Translation: CAB81020.1.
CP002687 Genomic DNA. Translation: AEE85745.1.
AY035156 mRNA. Translation: AAK59660.1.
AY063027 mRNA. Translation: AAL34201.1.
AY085867 mRNA. Translation: AAM63080.1.
Z17602 mRNA. Translation: CAA79012.1. Frameshift.
AF035384 mRNA. Translation: AAC39467.1.
X82683 Genomic DNA. Translation: CAA58001.1.
PIRiS61555.
T51754.
RefSeqiNP_194756.1. NM_119173.3.
UniGeneiAt.20967.
At.26243.
At.27681.
At.47568.
At.75103.

Genome annotation databases

EnsemblPlantsiAT4G30270.1; AT4G30270.1; AT4G30270.
GeneIDi829150.
KEGGiath:AT4G30270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63166 Genomic DNA. Translation: AAA32828.1. Frameshift.
D63508 mRNA. Translation: BAA09783.1.
AL109796 Genomic DNA. Translation: CAB52471.1.
AL161576 Genomic DNA. Translation: CAB81020.1.
CP002687 Genomic DNA. Translation: AEE85745.1.
AY035156 mRNA. Translation: AAK59660.1.
AY063027 mRNA. Translation: AAL34201.1.
AY085867 mRNA. Translation: AAM63080.1.
Z17602 mRNA. Translation: CAA79012.1. Frameshift.
AF035384 mRNA. Translation: AAC39467.1.
X82683 Genomic DNA. Translation: CAA58001.1.
PIRiS61555.
T51754.
RefSeqiNP_194756.1. NM_119173.3.
UniGeneiAt.20967.
At.26243.
At.27681.
At.47568.
At.75103.

3D structure databases

ProteinModelPortaliP24806.
SMRiP24806. Positions 46-256.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbiP24806.
PRIDEiP24806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G30270.1; AT4G30270.1; AT4G30270.
GeneIDi829150.
KEGGiath:AT4G30270.

Organism-specific databases

TAIRiAT4G30270.

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiP24806.
KOiK08235.
OMAiMASYRNI.
PhylomeDBiP24806.

Enzyme and pathway databases

BRENDAi2.4.1.207. 399.

Gene expression databases

ExpressionAtlasiP24806. baseline and differential.
GenevestigatoriP24806.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of genes expressed in shoot apical meristems."
    Medford J.I., Elmer J.S., Klee H.J.
    Plant Cell 3:359-370(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
  2. Kamimai T., Tomita E., Nishitani K.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-132.
    Strain: cv. Columbia.
    Tissue: Green siliques.
  8. "Differential expression of senescence-associated mRNAs during leaf senescence induced by different senescence-inducing factors in Arabidopsis."
    Park J.-H., Oh S.A., Kim Y.H., Woo H.R., Nam H.G.
    Plant Mol. Biol. 37:445-454(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-120.
    Strain: cv. Columbia.
    Tissue: Leaf.
  9. "Characterisation of two tomato fruit-expressed cDNAs encoding xyloglucan endo-transglycosylase."
    Arrowsmith D.A., De Silva J.
    Plant Mol. Biol. 28:391-403(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 104-269.
    Strain: cv. Landsberg erecta.
  10. "In vitro activities of four xyloglucan endotransglycosylases from Arabidopsis."
    Campbell P., Braam J.
    Plant J. 18:371-382(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME ACTIVITY, GLYCOSYLATION.
  11. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
    Yokoyama R., Nishitani K.
    Plant Cell Physiol. 42:1025-1033(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  12. "The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation."
    Kim G.-T., Shoda K., Tsuge T., Cho K.-H., Uchimiya H., Yokoyama R., Nishitani K., Tsukaya H.
    EMBO J. 21:1267-1279(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  13. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
    Rose J.K.C., Braam J., Fry S.C., Nishitani K.
    Plant Cell Physiol. 43:1421-1435(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiXTH24_ARATH
AccessioniPrimary (citable) accession number: P24806
Secondary accession number(s): O64956
, Q39148, Q39149, Q41904, Q8LDQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 29, 1992
Last sequence update: July 10, 2002
Last modified: March 31, 2015
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.