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P24806 (XTH24_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Xyloglucan endotransglucosylase/hydrolase protein 24

Short name=At-XTH24
Short name=XTH-24
EC=2.4.1.207
Alternative name(s):
Endo-xyloglucan transferase
Meristem protein 5
Short name=MERI-5 protein
Short name=MERI5 protein
Xyloglucan endo-1,4-beta-D-glucanase
Gene names
Name:XTH24
Synonyms:MERI-5, MERI5B, SEN4
Ordered Locus Names:At4g30270
ORF Names:F9N11.120
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues. May be required during development to modify the walls of cells under mechanical stress.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan. Ref.10

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Tissue specificity

Highly expressed. Predominantly expressed in stems. Expressed in shoot apical meristems, also found in seedlings and meristems. Ref.1 Ref.11

Induction

May be transcriptionally regulated by ANGUSTIFOLIA. Ref.12

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

N-glycosylated; essential for its enzymatic activity. Ref.10

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 2 subfamily.

Sequence caution

The sequence AAA32828.1 differs from that shown. Reason: Frameshift at positions 158, 178, 183, 189, 190, 194 and 199.

The sequence CAA79012.1 differs from that shown. Reason: Frameshift at positions 93 and 104.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 269248Xyloglucan endotransglucosylase/hydrolase protein 24
PRO_0000011824

Sites

Active site981Nucleophile By similarity
Active site1021Proton donor By similarity

Amino acid modifications

Glycosylation1061N-linked (GlcNAc...) Probable

Experimental info

Sequence conflict651L → F in AAM63080. Ref.6
Sequence conflict93 – 942GS → DR in AAC39467. Ref.8
Sequence conflict1841A → G Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24806 [UniParc].

Last modified July 11, 2002. Version 2.
Checksum: 648F042BC7ADED86

FASTA26930,756
        10         20         30         40         50         60 
MSPFKIFFFT TLLVAAFSVS AADFNTDVNV AWGNGRGKIL NNGQLLTLSL DKSSGSGFQS 

        70         80         90        100        110        120 
KTEYLFGKID MQIKLVPGNS AGTVTTFYLK SEGSTWDEID FEFLGNMSGD PYTLHTNVYT 

       130        140        150        160        170        180 
QGKGDKEQQF HLWFDPTANF HTYSILWNPQ RIILTVDDTP IREFKNYESL GVLFPKNKPM 

       190        200        210        220        230        240 
RMYASLWNAD DWATRGGLVK TDWSKAPFMA SYRNIKIDSK PNSNWYTQEM DSTSQARLKW 

       250        260 
VQKNYMIYNY CTDHRRFPQG APKECTTSS 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of genes expressed in shoot apical meristems."
Medford J.I., Elmer J.S., Klee H.J.
Plant Cell 3:359-370(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
[2]Kamimai T., Tomita E., Nishitani K.
Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana."
Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M., Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D., Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M., Cooke R. expand/collapse author list , Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C., Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B.
Plant J. 4:1051-1061(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-132.
Strain: cv. Columbia.
Tissue: Green siliques.
[8]"Differential expression of senescence-associated mRNAs during leaf senescence induced by different senescence-inducing factors in Arabidopsis."
Park J.-H., Oh S.A., Kim Y.H., Woo H.R., Nam H.G.
Plant Mol. Biol. 37:445-454(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-120.
Strain: cv. Columbia.
Tissue: Leaf.
[9]"Characterisation of two tomato fruit-expressed cDNAs encoding xyloglucan endo-transglycosylase."
Arrowsmith D.A., De Silva J.
Plant Mol. Biol. 28:391-403(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 104-269.
Strain: cv. Landsberg erecta.
[10]"In vitro activities of four xyloglucan endotransglycosylases from Arabidopsis."
Campbell P., Braam J.
Plant J. 18:371-382(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME ACTIVITY, GLYCOSYLATION.
[11]"A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
Yokoyama R., Nishitani K.
Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[12]"The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation."
Kim G.-T., Shoda K., Tsuge T., Cho K.-H., Uchimiya H., Yokoyama R., Nishitani K., Tsukaya H.
EMBO J. 21:1267-1279(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[13]"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
Rose J.K.C., Braam J., Fry S.C., Nishitani K.
Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M63166 Genomic DNA. Translation: AAA32828.1. Frameshift.
D63508 mRNA. Translation: BAA09783.1.
AL109796 Genomic DNA. Translation: CAB52471.1.
AL161576 Genomic DNA. Translation: CAB81020.1.
CP002687 Genomic DNA. Translation: AEE85745.1.
AY035156 mRNA. Translation: AAK59660.1.
AY063027 mRNA. Translation: AAL34201.1.
AY085867 mRNA. Translation: AAM63080.1.
Z17602 mRNA. Translation: CAA79012.1. Frameshift.
AF035384 mRNA. Translation: AAC39467.1.
X82683 Genomic DNA. Translation: CAA58001.1.
PIRS61555.
T51754.
RefSeqNP_194756.1. NM_119173.3.
UniGeneAt.20967.
At.26243.
At.27681.
At.47568.
At.75103.

3D structure databases

ProteinModelPortalP24806.
SMRP24806. Positions 22-268.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbP24806.
PRIDEP24806.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G30270.1; AT4G30270.1; AT4G30270.
GeneID829150.
KEGGath:AT4G30270.

Organism-specific databases

TAIRAT4G30270.

Phylogenomic databases

eggNOGCOG2273.
HOGENOMHOG000236368.
InParanoidP24806.
KOK08235.
OMAMASYRNI.
PhylomeDBP24806.
ProtClustDBCLSN2915933.

Gene expression databases

ArrayExpressP24806.
GenevestigatorP24806.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXTH24_ARATH
AccessionPrimary (citable) accession number: P24806
Secondary accession number(s): O64956 expand/collapse secondary AC list , Q39148, Q39149, Q41904, Q8LDQ0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 11, 2002
Last modified: April 16, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names