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Protein

Xyloglucan endotransglucosylase/hydrolase protein 24

Gene

XTH24

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues. May be required during development to modify the walls of cells under mechanical stress.

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98NucleophilePROSITE-ProRule annotation1
Active sitei102Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cell wall biogenesis Source: InterPro
  • plant-type cell wall loosening Source: TAIR
  • plant-type cell wall organization or biogenesis Source: TAIR
  • xyloglucan metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BRENDAi2.4.1.207. 399.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglucosylase/hydrolase protein 24 (EC:2.4.1.207)
Short name:
At-XTH24
Short name:
XTH-24
Alternative name(s):
Endo-xyloglucan transferase
Meristem protein 5
Short name:
MERI-5 protein
Short name:
MERI5 protein
Xyloglucan endo-1,4-beta-D-glucanase
Gene namesi
Name:XTH24
Synonyms:MERI-5, MERI5B, SEN4
Ordered Locus Names:At4g30270
ORF Names:F9N11.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G30270.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: UniProtKB-SubCell
  • cell wall Source: TAIR
  • cytoplasm Source: TAIR
  • Golgi apparatus Source: TAIR
  • plant-type cell wall Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001182422 – 269Xyloglucan endotransglucosylase/hydrolase protein 24Add BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi106N-linked (GlcNAc...)1 Publication1

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity
N-glycosylated; essential for its enzymatic activity.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP24806.

Expressioni

Tissue specificityi

Highly expressed. Predominantly expressed in stems. Expressed in shoot apical meristems, also found in seedlings and meristems.2 Publications

Inductioni

May be transcriptionally regulated by ANGUSTIFOLIA.1 Publication

Gene expression databases

ExpressionAtlasiP24806. baseline and differential.
GenevisibleiP24806. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G30270.1.

Structurei

3D structure databases

ProteinModelPortaliP24806.
SMRiP24806.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 212GH16PROSITE-ProRule annotationAdd BLAST191

Sequence similaritiesi

Contains 1 GH16 (glycosyl hydrolase family 16) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JBCJ. Eukaryota.
COG2273. LUCA.
HOGENOMiHOG000236368.
InParanoidiP24806.
KOiK08235.
OMAiWSKAPFM.
OrthoDBiEOG09360GR5.
PhylomeDBiP24806.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
IPR010713. XET_C.
IPR016455. XTH.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24806-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPFKIFFFT TLLVAAFSVS AADFNTDVNV AWGNGRGKIL NNGQLLTLSL
60 70 80 90 100
DKSSGSGFQS KTEYLFGKID MQIKLVPGNS AGTVTTFYLK SEGSTWDEID
110 120 130 140 150
FEFLGNMSGD PYTLHTNVYT QGKGDKEQQF HLWFDPTANF HTYSILWNPQ
160 170 180 190 200
RIILTVDDTP IREFKNYESL GVLFPKNKPM RMYASLWNAD DWATRGGLVK
210 220 230 240 250
TDWSKAPFMA SYRNIKIDSK PNSNWYTQEM DSTSQARLKW VQKNYMIYNY
260
CTDHRRFPQG APKECTTSS
Length:269
Mass (Da):30,756
Last modified:July 11, 2002 - v2
Checksum:i648F042BC7ADED86
GO

Sequence cautioni

The sequence AAA32828 differs from that shown. Reason: Frameshift at positions 158, 178, 183, 189, 190, 194 and 199.Curated
The sequence CAA79012 differs from that shown. Reason: Frameshift at positions 93 and 104.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65L → F in AAM63080 (Ref. 6) Curated1
Sequence conflicti93 – 94GS → DR in AAC39467 (PubMed:9617812).Curated2
Sequence conflicti184A → G (PubMed:1840916).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63166 Genomic DNA. Translation: AAA32828.1. Frameshift.
D63508 mRNA. Translation: BAA09783.1.
AL109796 Genomic DNA. Translation: CAB52471.1.
AL161576 Genomic DNA. Translation: CAB81020.1.
CP002687 Genomic DNA. Translation: AEE85745.1.
AY035156 mRNA. Translation: AAK59660.1.
AY063027 mRNA. Translation: AAL34201.1.
AY085867 mRNA. Translation: AAM63080.1.
Z17602 mRNA. Translation: CAA79012.1. Frameshift.
AF035384 mRNA. Translation: AAC39467.1.
X82683 Genomic DNA. Translation: CAA58001.1.
PIRiS61555.
T51754.
RefSeqiNP_194756.1. NM_119173.4.
UniGeneiAt.20967.
At.26243.
At.27681.
At.47568.
At.75103.

Genome annotation databases

EnsemblPlantsiAT4G30270.1; AT4G30270.1; AT4G30270.
GeneIDi829150.
GrameneiAT4G30270.1; AT4G30270.1; AT4G30270.
KEGGiath:AT4G30270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63166 Genomic DNA. Translation: AAA32828.1. Frameshift.
D63508 mRNA. Translation: BAA09783.1.
AL109796 Genomic DNA. Translation: CAB52471.1.
AL161576 Genomic DNA. Translation: CAB81020.1.
CP002687 Genomic DNA. Translation: AEE85745.1.
AY035156 mRNA. Translation: AAK59660.1.
AY063027 mRNA. Translation: AAL34201.1.
AY085867 mRNA. Translation: AAM63080.1.
Z17602 mRNA. Translation: CAA79012.1. Frameshift.
AF035384 mRNA. Translation: AAC39467.1.
X82683 Genomic DNA. Translation: CAA58001.1.
PIRiS61555.
T51754.
RefSeqiNP_194756.1. NM_119173.4.
UniGeneiAt.20967.
At.26243.
At.27681.
At.47568.
At.75103.

3D structure databases

ProteinModelPortaliP24806.
SMRiP24806.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G30270.1.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbiP24806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G30270.1; AT4G30270.1; AT4G30270.
GeneIDi829150.
GrameneiAT4G30270.1; AT4G30270.1; AT4G30270.
KEGGiath:AT4G30270.

Organism-specific databases

TAIRiAT4G30270.

Phylogenomic databases

eggNOGiENOG410JBCJ. Eukaryota.
COG2273. LUCA.
HOGENOMiHOG000236368.
InParanoidiP24806.
KOiK08235.
OMAiWSKAPFM.
OrthoDBiEOG09360GR5.
PhylomeDBiP24806.

Enzyme and pathway databases

BRENDAi2.4.1.207. 399.

Miscellaneous databases

PROiP24806.

Gene expression databases

ExpressionAtlasiP24806. baseline and differential.
GenevisibleiP24806. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
IPR010713. XET_C.
IPR016455. XTH.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXTH24_ARATH
AccessioniPrimary (citable) accession number: P24806
Secondary accession number(s): O64956
, Q39148, Q39149, Q41904, Q8LDQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 11, 2002
Last modified: November 30, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.