Reviewed, UniProtKB/Swiss-Prot P24791 (PME2_RALSO)

Names and origin

Protein names

Recommended name:
    Pectinesterase
      Short name=PE
    EC=3.1.1.11
Alternative name(s):
    Pectin methylesterase

Gene names

Name:

pme

Organism

Ralstonia solanacearum (Pseudomonas solanacearum)

Taxonomic identifier

305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconic acid from pectin: step 1/5.
Subcellular location	Secreted.
Sequence similarities	Belongs to the pectinesterase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 396	370	Pectinesterase		PRO_0000023501
Sites
	Active site	232	1	Proton donor By similarity
	Active site	259	1	Nucleophile By similarity
	Binding site	174	1	Substrate By similarity
	Binding site	324	1	Substrate By similarity
	Binding site	326	1	Substrate By similarity
	Site	231	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24791-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 82E2AFC4C0B54AB4	FASTA	396	41,016
	10 20 30 40 50 60 MQSTTLYLKT AAFLGGCSLF AATALAATST ATRPQLSNAD ARAYTIASYM ASFGTIGSLT 70 80 90 100 110 120 TDNWDPTGGV GAVSGFRANY AVAADGSAQY KTVQAAIDAA VADGGVARKY ISVKAGTYNE 130 140 150 160 170 180 LVCVPESAPP ITLYSLDANA NNTVIVYNNA NPTPASGAKT NPCMGTSSNA TVGTVRSATA 190 200 210 220 230 240 MVRASNFNAR NLTFKNSYVE GTFADNNQSA VALAVRGDKA ILENVSVIGN QDTLYLGATN 250 260 270 280 290 300 NTMVIRAYFK NSFIQGDTDF IFGAGTAVFH GCTIQYTAAR LGARATSYVF APSTAPDNPH 310 320 330 340 350 360 GFLAINSTFN ATGNASNNST HLGRAWDQGV SGTSAYINGS SPNGQVVIRD SSLGAHIRLA 370 380 390 DPWGPSTAGR PYCSSKCAYS ANRFFEYNNT GAGSGN

References

[1]	"Molecular cloning and sequencing of a pectinesterase gene from Pseudomonas solanacearum." Spoek A., Stubenrauch G., Schoergendorfer K., Schwab H. J. Gen. Microbiol. 137:131-140(1991) [PubMed: 2045776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 50905.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M62803 Genomic DNA. Translation: AAA25984.1.
PIR	A49747.
3D structure databases
HSSP	HSSP built from PDB template 1GQ8 based on UniProtKB P83218.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.1.11. 97066.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
PROSITE	PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME2_RALSO

Accession

Primary (citable) accession number: P24791

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)