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Reviewed, UniProtKB/Swiss-Prot P24788 (CD2L1_MOUSE)

Last modified June 16, 2009. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    PITSLRE serine/threonine-protein kinase CDC2L1
    EC=2.7.11.22
Alternative name(s):
    Cell division cycle 2-like protein kinase 1
    Galactosyltransferase-associated protein kinase p58/GTA
Gene names
Name: Cdc2l1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length784 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Acts as a negative regulator of the normal cell cycle progression. In vitro, this protein kinase has been shown to phosphorylate a number of substrates, including histone h1, casein, and galactosyltransferase. May function in regulating proliferation by the phosphorylation and subsequent plasma membrane targeting of galactosyltransferase.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium.

Enzyme regulation

Phosphorylation at Thr-437 or Tyr-438 inactivates the enzyme, while phosphorylation at Thr-584 activates it By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAA03518.1 differs from that shown. Reason: Frameshift at several positions.

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform 1 (identifier: P24788-1)

Also known as: p130PITSLRE;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P24788-2)

Also known as: p58clk-1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-345: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 784784PITSLRE serine/threonine-protein kinase CDC2L1
PRO_0000024313

Regions

Domain427 – 712286Protein kinase
Nucleotide binding433 – 4419ATP By similarity
Compositional bias291 – 30414Poly-Glu
Compositional bias309 – 32517Poly-Glu

Sites

Active site5511Proton acceptor By similarity
Binding site4561ATP By similarity

Amino acid modifications

Modified residue651Phosphoserine By similarity
Modified residue2701Phosphoserine Ref.5 Ref.6
Modified residue3701Phosphoserine By similarity
Modified residue3731Phosphoserine By similarity
Modified residue3841Phosphothreonine By similarity
Modified residue3991Phosphoserine By similarity
Modified residue4031Phosphoserine By similarity
Modified residue4371Phosphothreonine By similarity
Modified residue4381Phosphotyrosine By similarity
Modified residue5781Phosphoserine By similarity
Modified residue5831Phosphotyrosine By similarity
Modified residue5841Phosphothreonine By similarity
Modified residue7401Phosphothreonine Ref.7
Modified residue7411Phosphoserine Ref.7

Natural variations

Alternative sequence1 – 345345Missing in isoform 2.
VSP_018835

Experimental info

Sequence conflict35 – 373LKN → MSQ in AAH52920. Ref.4
Sequence conflict2841Missing in AAA66169. Ref.2
Sequence conflict5601S → T in AAA03518. Ref.1
Sequence conflict6081V → C in AAA03518. Ref.1
Sequence conflict6451T → S in AAA03518. Ref.1
Sequence conflict6681Y → I in AAA03518. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (p130PITSLRE) [UniParc].

Last modified January 4, 2005. Version 2.
Checksum: CDF03AC3957FA351

FASTA78491,513
        10         20         30         40         50         60 
MGDEKDSWKV KTLDEILQEK KRRKEQEEKA EIKRLKNSDD RDSKRDSLEE GELRDHRMEI 

        70         80         90        100        110        120 
TIRNSPYRRE DSMEDRGEED DSLAIKPPQQ MSRKEKAHHR KDEKRKEKRR HRSHSAEGGK 

       130        140        150        160        170        180 
HARVKEKERE HERRKRHREE QDKARREWER QKRREMAREH SRRERDRLEQ LERKRERERK 

       190        200        210        220        230        240 
LREQQKEQRE QKERERRAEE RRKEREARRE VSAHHRTMRE EYSDKGKVGH WSRSPLRPPR 

       250        260        270        280        290        300 
ERFEMGDNRK PVKEEKVEER DLLSDLQDIS DSERKTSSAE SSSAESGSGS EEEEEEEEEE 

       310        320        330        340        350        360 
EEEEGSTSEE SEEEEEEEEE EEEEETGSNS EEASEQSAEE VSDEEMSEDE DRENENHILV 

       370        380        390        400        410        420 
VPESRFDRDS GDSEEGEEEV GEGTPQSSAP TEGDYVPDSP ALSPIELKQE LPKYLPALQG 

       430        440        450        460        470        480 
CRSVEEFQCL NRIEEGTYGV VYRAKDKKTD EIVALKRLKM EKEKEGFPIT SLREINTILK 

       490        500        510        520        530        540 
AQHPNIVTVR EIVVGSNMDK IYIVMNYVEH DLKSLMETMK QPFLPGEVKT LMIQLLSGVK 

       550        560        570        580        590        600 
HLHDNWILHR DLKTSNLLLS HAGILKVGDF GLAREYGSPL KAYTPVVVTL WYRAPELLLG 

       610        620        630        640        650        660 
AKEYSTAVDM WSVGCIFGEL LTQKPLFPGK SDIDQINKIF KDLGTPSEKI WPGYNDLPAV 

       670        680        690        700        710        720 
KKMTFSEYPY NNLRKRFGAL LSDQGFDLMN KFLTYYPGRR INAEDGLKHE YFRETPLPID 

       730        740        750        760        770        780 
PSMFPTWPAK SEQQRVKRGT SPRPPEGGLG YSQLGDDDLK ETGFHLTTTN QGASAAGPGF 


SLKF 

« Hide

Isoform 2 (p58clk-1).

Checksum: A31D79C4919C1A3B
Show »

FASTA43949,509

References

« Hide 'large scale' references
[1]"Regulated expression of a cell division control-related protein kinase during development."
Kidd V.J., Luo W., Xiang J.L., Tu F., Easton J., McCune S., Snead M.L.
Cell Growth Differ. 2:85-93(1991) [PubMed: 2069872] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"A cyclin-dependent kinase homologue, p130PITSLRE is a phosphotyrosine-independent SH2 ligand."
Malek S.N., Desiderio S.
J. Biol. Chem. 269:33009-33020(1994) [PubMed: 7528743] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Stomach.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 35-784 (ISOFORM 1).
Tissue: Eye.
[5]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed: 15345747] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270, MASS SPECTROMETRY.
Tissue: Brain.
[6]"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry."
Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.
J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270, MASS SPECTROMETRY.
Tissue: Liver.
[7]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-740 AND SER-741, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

M58633 mRNA. Translation: AAA03518.1. Frameshift.
L37092 mRNA. Translation: AAA66169.1.
AK077668 mRNA. Translation: BAC36942.1.
AK147133 mRNA. Translation: BAE27703.1.
BC052920 mRNA. Translation: AAH52920.1.
IPIIPI00110050.
IPI00649424.
PIRA55817.
RefSeqNP_031687.2.
UniGeneMm.267410

3D structure databases

HSSPHSSP built from PDB template 1H00 based on UniProtKB P24941.
ModBaseSearch...

PTM databases

PhosphoSiteP24788.

Proteomic databases

PRIDEP24788.

Genome annotation databases

EnsemblENSMUSG00000029062. Mus musculus. [Contig view]
GeneID12537.
KEGGmmu:12537.

Organism-specific databases

MGIMGI:88353. Cdc2l1.

Phylogenomic databases

HOGENOMP24788.
HOVERGENP24788.
OMAP24788. EGDFVPD.

Enzyme and pathway databases

BRENDA2.7.11.22. 244.

Gene expression databases

BgeeP24788.
CleanExMM_CDC2L1.
GermOnlineENSMUSG00000029062. Mus musculus.

Family and domain databases

InterProIPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio281574.
SOURCESearch...

Entry information

Entry nameCD2L1_MOUSE
AccessionPrimary (citable) accession number: P24788
Secondary accession number(s): Q3UI03 expand/collapse secondary AC list , Q61399, Q7TST4, Q8BP53
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 4, 2005
Last modified: June 16, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents