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Protein

Dosage compensation regulator

Gene

mle

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required in males for dosage compensation of X chromosome linked genes. Mle, msl-1 and msl-3 are colocalized on X chromosome. Each of the msl proteins requires all the other msls for wild-type X-chromosome binding. Probably unwinds double-stranded DNA and RNA in a 3' to 5' direction.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi407 – 414ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent helicase activity Source: FlyBase
  • ATP-dependent RNA helicase activity Source: GO_Central
  • chromatin binding Source: FlyBase
  • double-stranded RNA binding Source: FlyBase
  • helicase activity Source: FlyBase
  • poly(A) RNA binding Source: GO_Central
  • RNA binding Source: FlyBase
  • RNA helicase activity Source: FlyBase

GO - Biological processi

  • axon extension Source: FlyBase
  • determination of adult lifespan Source: FlyBase
  • dosage compensation Source: FlyBase
  • dosage compensation by hyperactivation of X chromosome Source: FlyBase
  • dosage compensation complex assembly involved in dosage compensation by hyperactivation of X chromosome Source: FlyBase
  • male courtship behavior, veined wing generated song production Source: FlyBase
  • positive regulation of heterochromatin assembly Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • RNA processing Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-1810476. RIP-mediated NFkB activation via ZBP1.
R-DME-3134963. DEx/H-box helicases activate type I IFN and inflammatory cytokines production.
R-DME-445989. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-DME-72163. mRNA Splicing - Major Pathway.
R-DME-933542. TRAF6 mediated NF-kB activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Dosage compensation regulator (EC:3.6.4.13)
Alternative name(s):
ATP-dependent RNA helicase mle
Protein male-less
Protein maleless
Protein no action potential
Gene namesi
Name:mleImported
Synonyms:napImported
ORF Names:CG11680Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0002774. mle.

Subcellular locationi

  • Nucleus 1 Publication
  • Chromosome 1 Publication

  • Note: Mle is associated with hundreds of discrete sites along the length of the X chromosome in males and not in females, and is associated with 30-40 autosomal sites in both sexes.

GO - Cellular componenti

  • chromatin Source: FlyBase
  • chromosome Source: FlyBase
  • MSL complex Source: FlyBase
  • nuclear chromosome Source: FlyBase
  • polytene chromosome Source: FlyBase
  • X chromosome Source: FlyBase
  • X chromosome located dosage compensation complex, transcription activating Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551811 – 1293Dosage compensation regulatorAdd BLAST1293

Proteomic databases

PaxDbiP24785.
PRIDEiP24785.

Expressioni

Developmental stagei

Expressed throughout development; highest in embryos and at equal levels in males and females.1 Publication

Gene expression databases

BgeeiFBgn0002774.
ExpressionAtlasiP24785. baseline.
GenevisibleiP24785. DM.

Interactioni

Subunit structurei

Interacts with Top2.1 Publication

Protein-protein interaction databases

BioGridi61429. 14 interactors.
IntActiP24785. 6 interactors.
MINTiMINT-768581.
STRINGi7227.FBpp0085367.

Structurei

Secondary structure

11293
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni121 – 123Combined sources3
Helixi139 – 144Combined sources6
Helixi147 – 154Combined sources8
Helixi159 – 161Combined sources3
Turni162 – 164Combined sources3
Helixi167 – 180Combined sources14
Beta strandi188 – 193Combined sources6
Helixi195 – 197Combined sources3
Beta strandi199 – 208Combined sources10
Helixi209 – 211Combined sources3
Beta strandi213 – 223Combined sources11
Helixi224 – 241Combined sources18
Helixi269 – 281Combined sources13
Helixi289 – 291Combined sources3
Beta strandi296 – 298Combined sources3
Turni337 – 340Combined sources4
Beta strandi345 – 350Combined sources6
Helixi353 – 370Combined sources18
Helixi372 – 382Combined sources11
Helixi385 – 389Combined sources5
Helixi390 – 399Combined sources10
Beta strandi401 – 407Combined sources7
Helixi413 – 427Combined sources15
Helixi431 – 433Combined sources3
Beta strandi435 – 442Combined sources8
Helixi443 – 456Combined sources14
Beta strandi463 – 469Combined sources7
Beta strandi472 – 474Combined sources3
Beta strandi478 – 486Combined sources9
Helixi487 – 493Combined sources7
Helixi494 – 496Combined sources3
Beta strandi503 – 508Combined sources6
Helixi509 – 511Combined sources3
Helixi514 – 529Combined sources16
Beta strandi534 – 541Combined sources8
Helixi544 – 550Combined sources7
Beta strandi556 – 559Combined sources4
Beta strandi566 – 569Combined sources4
Helixi571 – 578Combined sources8
Helixi584 – 600Combined sources17
Helixi606 – 608Combined sources3
Helixi611 – 613Combined sources3
Helixi621 – 629Combined sources9
Beta strandi632 – 634Combined sources3
Helixi637 – 649Combined sources13
Beta strandi655 – 659Combined sources5
Helixi663 – 674Combined sources12
Turni677 – 680Combined sources4
Turni682 – 684Combined sources3
Beta strandi685 – 690Combined sources6
Helixi696 – 700Combined sources5
Turni701 – 703Combined sources3
Beta strandi711 – 716Combined sources6
Helixi718 – 720Combined sources3
Beta strandi721 – 723Combined sources3
Beta strandi729 – 734Combined sources6
Beta strandi737 – 744Combined sources8
Turni745 – 748Combined sources4
Beta strandi749 – 756Combined sources8
Helixi759 – 766Combined sources8
Beta strandi769 – 773Combined sources5
Beta strandi775 – 779Combined sources5
Helixi783 – 788Combined sources6
Helixi796 – 799Combined sources4
Helixi803 – 811Combined sources9
Helixi817 – 822Combined sources6
Beta strandi824 – 826Combined sources3
Helixi830 – 842Combined sources13
Helixi854 – 860Combined sources7
Beta strandi862 – 864Combined sources3
Helixi866 – 877Combined sources12
Helixi881 – 890Combined sources10
Helixi909 – 912Combined sources4
Helixi913 – 915Combined sources3
Helixi921 – 937Combined sources17
Helixi940 – 950Combined sources11
Helixi954 – 973Combined sources20
Helixi978 – 981Combined sources4
Helixi995 – 1007Combined sources13
Turni1008 – 1010Combined sources3
Beta strandi1012 – 1017Combined sources6
Beta strandi1020 – 1023Combined sources4
Helixi1024 – 1026Combined sources3
Beta strandi1027 – 1031Combined sources5
Beta strandi1049 – 1069Combined sources21
Helixi1071 – 1077Combined sources7
Beta strandi1082 – 1084Combined sources3
Helixi1086 – 1088Combined sources3
Beta strandi1090 – 1092Combined sources3
Turni1093 – 1095Combined sources3
Beta strandi1096 – 1100Combined sources5
Helixi1102 – 1124Combined sources23
Helixi1126 – 1128Combined sources3
Helixi1134 – 1147Combined sources14
Turni1149 – 1152Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AORX-ray2.08A/B1-1293[»]
ProteinModelPortaliP24785.
SMRiP24785.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 70DRBM 1PROSITE-ProRule annotationAdd BLAST69
Domaini169 – 241DRBM 2PROSITE-ProRule annotationAdd BLAST73
Domaini394 – 560Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST167
Domaini639 – 813Helicase C-terminalPROSITE-ProRule annotationAdd BLAST175
Repeati1202 – 120817
Repeati1209 – 121527
Repeati1216 – 122237
Repeati1223 – 122947
Repeati1230 – 123657
Repeati1237 – 124367
Repeati1244 – 125077
Repeati1251 – 125787
Repeati1258 – 126396

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1202 – 12639 X 7 AA tandem repeats of G-G-G-Y-G-N-NAdd BLAST62

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi507 – 510DEAH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1173 – 1291Gly-richAdd BLAST119

Sequence similaritiesi

Contains 2 DRBM (double-stranded RNA-binding) domains.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0920. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00760000119189.
InParanoidiP24785.
KOiK13184.
OMAiCSDHVAM.
OrthoDBiEOG091G0PKT.
PhylomeDBiP24785.

Family and domain databases

Gene3Di3.30.160.20. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR014720. dsRBD_dom.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00035. dsrm. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00358. DSRM. 2 hits.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS50137. DS_RBD. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform A (identifier: P24785-1) [UniParc]FASTAAdd to basket
Also known as: 25

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDIKSFLYQF CAKSQIEPKF DIRQTGPKNR QRFLCEVRVE PNTYIGVGNS
60 70 80 90 100
TNKKDAEKNA CRDFVNYLVR VGKLNTNDVP ADAGASGGGP RTGLEGAGMA
110 120 130 140 150
GGSGQQKRVF DGQSGPQDLG EAYRPLNHDG GDGGNRYSVI DRIQEQRDMN
160 170 180 190 200
EAEAFDVNAA IHGNWTIENA KERLNIYKQT NNIRDDYKYT PVGPEHARSF
210 220 230 240 250
LAELSIYVPA LNRTVTARES GSNKKSASKS CALSLVRQLF HLNVIEPFSG
260 270 280 290 300
TLKKKKDEQL KPYPVKLSPN LINKIDEVIK GLDLPVVNPR NIKIELDGPP
310 320 330 340 350
IPLIVNLSRI DSSQQDGEKR QESSVIPWAP PQANWNTWHA CNIDEGELAT
360 370 380 390 400
TSIDDLSMDY ERSLRDRRQN DNEYRQFLEF REKLPIAAMR SEILTAINDN
410 420 430 440 450
PVVIIRGNTG CGKTTQIAQY ILDDYICSGQ GGYANIYVTQ PRRISAISVA
460 470 480 490 500
ERVARERCEQ LGDTVGYSVR FESVFPRPYG AILFCTVGVL LRKLEAGLRG
510 520 530 540 550
VSHIIVDEIH ERDVNSDFLL VILRDMVDTY PDLHVILMSA TIDTTKFSKY
560 570 580 590 600
FGICPVLEVP GRAFPVQQFF LEDIIQMTDF VPSAESRRKR KEVEDEEQLL
610 620 630 640 650
SEDKDEAEIN YNKVCEDKYS QKTRNAMAML SESDVSFELL EALLMHIKSK
660 670 680 690 700
NIPGAILVFL PGWNLIFALM KFLQNTNIFG DTSQYQILPC HSQIPRDEQR
710 720 730 740 750
KVFEPVPEGV TKIILSTNIA ETSITIDDIV FVIDICKARM KLFTSHNNLT
760 770 780 790 800
SYATVWASKT NLEQRKGRAG RVRPGFCFTL CSRARFQALE DNLTPEMFRT
810 820 830 840 850
PLHEMALTIK LLRLGSIHHF LSKALEPPPV DAVIEAEVLL REMRCLDAND
860 870 880 890 900
ELTPLGRLLA RLPIEPRLGK MMVLGAVFGC ADLMAIMASY SSTFSEVFSL
910 920 930 940 950
DIGQRRLANH QKALSGTKCS DHVAMIVASQ MWRREKQRGE HMEARFCDWK
960 970 980 990 1000
GLQMSTMNVI WDAKQQLLDL LQQAGFPEEC MISHEVDERI DGDDPVLDVS
1010 1020 1030 1040 1050
LALLCLGLYP NICVHKEKRK VLTTESKAAL LHKTSVNCSN LAVTFPYPFF
1060 1070 1080 1090 1100
VFGEKIRTRA VSCKQLSMVS PLQVILFGSR KIDLAANNIV RVDNWLNFDI
1110 1120 1130 1140 1150
EPELAAKIGA LKPALEDLIT VACDNPSDIL RLEEPYAQLV KVVKDLCVKS
1160 1170 1180 1190 1200
AGDFGLQRES GILPHQSRQF SDGGGPPKRG RFETGRFTNS SFGRRGNGRT
1210 1220 1230 1240 1250
FGGGYGNNGG GYGNNGGGYG NIGGGYGNNA GGYGNNGGYG NNGGGYRNNG
1260 1270 1280 1290
GGYGNNGGGY GNKRGGFGDS FESNRGSGGG FRNGDQGGRW GNF
Length:1,293
Mass (Da):143,661
Last modified:September 27, 2005 - v2
Checksum:i7667679F069BCAF5
GO
Isoform 12 (identifier: P24785-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     199-226: SFLAELSIYVPALNRTVTARESGSNKKS → YVLPFQLSSACISDLTRYGLRPNLKCSP
     227-1293: Missing.

Show »
Length:226
Mass (Da):25,083
Checksum:i32C6CFA40B0F84AF
GO
Isoform B (identifier: P24785-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-357: Missing.

Note: No experimental confirmation available.
Show »
Length:936
Mass (Da):104,129
Checksum:i608D316CAE0E2472
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti590R → P in AAC41573 (PubMed:1653648).Curated1
Sequence conflicti1263K → N in AAC41573 (PubMed:1653648).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti1276G → V.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0156901 – 357Missing in isoform B. 1 PublicationAdd BLAST357
Alternative sequenceiVSP_005775199 – 226SFLAE…SNKKS → YVLPFQLSSACISDLTRYGL RPNLKCSP in isoform 12. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_005776227 – 1293Missing in isoform 12. 1 PublicationAdd BLAST1067

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74121 mRNA. Translation: AAC41573.1.
AE013599 Genomic DNA. Translation: AAF57297.1.
AE013599 Genomic DNA. Translation: AAM68335.1.
BT003785 mRNA. Translation: AAO41468.1.
BT010267 mRNA. Translation: AAQ23585.1.
PIRiB40025.
RefSeqiNP_476641.1. NM_057293.4. [P24785-1]
NP_724440.1. NM_165451.3. [P24785-3]
UniGeneiDm.2901.

Genome annotation databases

EnsemblMetazoaiFBtr0086031; FBpp0085367; FBgn0002774. [P24785-1]
GeneIDi35523.
KEGGidme:Dmel_CG11680.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74121 mRNA. Translation: AAC41573.1.
AE013599 Genomic DNA. Translation: AAF57297.1.
AE013599 Genomic DNA. Translation: AAM68335.1.
BT003785 mRNA. Translation: AAO41468.1.
BT010267 mRNA. Translation: AAQ23585.1.
PIRiB40025.
RefSeqiNP_476641.1. NM_057293.4. [P24785-1]
NP_724440.1. NM_165451.3. [P24785-3]
UniGeneiDm.2901.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AORX-ray2.08A/B1-1293[»]
ProteinModelPortaliP24785.
SMRiP24785.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61429. 14 interactors.
IntActiP24785. 6 interactors.
MINTiMINT-768581.
STRINGi7227.FBpp0085367.

Proteomic databases

PaxDbiP24785.
PRIDEiP24785.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0086031; FBpp0085367; FBgn0002774. [P24785-1]
GeneIDi35523.
KEGGidme:Dmel_CG11680.

Organism-specific databases

CTDi35523.
FlyBaseiFBgn0002774. mle.

Phylogenomic databases

eggNOGiKOG0920. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00760000119189.
InParanoidiP24785.
KOiK13184.
OMAiCSDHVAM.
OrthoDBiEOG091G0PKT.
PhylomeDBiP24785.

Enzyme and pathway databases

ReactomeiR-DME-1810476. RIP-mediated NFkB activation via ZBP1.
R-DME-3134963. DEx/H-box helicases activate type I IFN and inflammatory cytokines production.
R-DME-445989. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-DME-72163. mRNA Splicing - Major Pathway.
R-DME-933542. TRAF6 mediated NF-kB activation.

Miscellaneous databases

GenomeRNAii35523.
PROiP24785.

Gene expression databases

BgeeiFBgn0002774.
ExpressionAtlasiP24785. baseline.
GenevisibleiP24785. DM.

Family and domain databases

Gene3Di3.30.160.20. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR014720. dsRBD_dom.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00035. dsrm. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00358. DSRM. 2 hits.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS50137. DS_RBD. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMLE_DROME
AccessioniPrimary (citable) accession number: P24785
Secondary accession number(s): Q86NQ4, Q9V9J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: September 27, 2005
Last modified: November 30, 2016
This is version 165 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.