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Protein

ATP-dependent RNA helicase DBP1

Gene

DBP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-binding RNA helicase involved in translation initiation. Remodels RNA in response to ADP and ATP concentrations by facilitating disruption, but also formation of RNA duplexes (By similarity). Redundant to DED1, may be required in conditions in which DED1 expression is decreased.By similarity2 Publications

Miscellaneous

Present with 1480 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi198 – 205ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • RNA secondary structure unwinding Source: GO_Central
  • translational initiation Source: SGD

Keywordsi

Molecular functionHelicase, Hydrolase, Initiation factor, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34018-MONOMER
ReactomeiR-SCE-1834941 STING mediated induction of host immune responses
R-SCE-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DBP1 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 1
Helicase CA1
Gene namesi
Name:DBP1
Ordered Locus Names:YPL119C
ORF Names:LPH8C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL119C
SGDiS000006040 DBP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550161 – 617ATP-dependent RNA helicase DBP1Add BLAST617

Proteomic databases

MaxQBiP24784
PaxDbiP24784
PRIDEiP24784

Interactioni

Protein-protein interaction databases

BioGridi36062, 100 interactors
DIPiDIP-2576N
IntActiP24784, 9 interactors
MINTiP24784
STRINGi4932.YPL119C

Structurei

3D structure databases

ProteinModelPortaliP24784
SMRiP24784
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini185 – 374Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST190
Domaini385 – 545Helicase C-terminalPROSITE-ProRule annotationAdd BLAST161

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi154 – 182Q motifAdd BLAST29
Motifi318 – 321DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00900000140933
HOGENOMiHOG000268804
InParanoidiP24784
KOiK11594
OMAiGPDWWGQ
OrthoDBiEOG092C1B4A

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

P24784-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLPQKVSN LSINNKENGG GGGKSSYVPP HLRSRGKPSF ERSTPKQEDK
60 70 80 90 100
VTGGDFFRRA GRQTGNNGGF FGFSKERNGG TSANYNRGGS SNYKSSGNRW
110 120 130 140 150
VNGKHIPGPK NAKLEAELFG VHDDPDYHSS GIKFDNYDNI PVDASGKDVP
160 170 180 190 200
EPILDFSSPP LDELLMENIK LASFTKPTPV QKYSIPIVTK GRDLMACAQT
210 220 230 240 250
GSGKTGGFLF PLFTELFRSG PSPVPEKAQS FYSRKGYPSA LVLAPTRELA
260 270 280 290 300
TQIFEEARKF TYRSWVRPCV VYGGAPIGNQ MREVDRGCDL LVATPGRLND
310 320 330 340 350
LLERGKVSLA NIKYLVLDEA DRMLDMGFEP QIRHIVEECD MPSVENRQTL
360 370 380 390 400
MFSATFPVDI QHLARDFLDN YIFLSVGRVG STSENITQRI LYVDDMDKKS
410 420 430 440 450
ALLDLLSAEH KGLTLIFVET KRMADQLTDF LIMQNFKATA IHGDRTQAER
460 470 480 490 500
ERALSAFKAN VADILVATAV AARGLDIPNV THVINYDLPS DIDDYVHRIG
510 520 530 540 550
RTGRAGNTGV ATSFFNSNNQ NIVKGLMEIL NEANQEVPTF LSDLSRQNSR
560 570 580 590 600
GGRTRGGGGF FNSRNNGSRD YRKHGGNGSF GSTRPRNTGT SNWGSIGGGF
610
RNDNEKNGYG NSNASWW
Length:617
Mass (Da):67,917
Last modified:October 1, 1996 - v2
Checksum:i8152404B7628671D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43 – 44ST → RS in CAA39465 (PubMed:1857205).Curated2
Sequence conflicti48E → K in CAA39465 (PubMed:1857205).Curated1
Sequence conflicti88G → R in CAA39465 (PubMed:1857205).Curated1
Sequence conflicti115E → QK in CAA39465 (PubMed:1857205).Curated1
Sequence conflicti496V → I no nucleotide entry (PubMed:2406722).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55993 Genomic DNA Translation: CAA39465.1
U43503 Genomic DNA Translation: AAB68243.1
BK006949 Genomic DNA Translation: DAA11315.1
PIRiS62003
RefSeqiNP_015206.1, NM_001183933.1

Genome annotation databases

EnsemblFungiiYPL119C; YPL119C; YPL119C
GeneIDi855984
KEGGisce:YPL119C

Similar proteinsi

Entry informationi

Entry nameiDBP1_YEAST
AccessioniPrimary (citable) accession number: P24784
Secondary accession number(s): D6W3P9, P20446
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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