Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent RNA helicase DBP2

Gene

DBP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase involved nonsense-mediated mRNA decay and ribosome biogenesis through rRNA processing.2 Publications

Miscellaneous

Present with 33100 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi157 – 164ATP8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • RNA binding Source: UniProtKB-KW
  • RNA-dependent ATPase activity Source: SGD

GO - Biological processi

  • messenger ribonucleoprotein complex assembly Source: SGD
  • nuclear polyadenylation-dependent mRNA catabolic process Source: SGD
  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: SGD
  • RNA secondary structure unwinding Source: GO_Central
  • rRNA processing Source: SGD

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processNonsense-mediated mRNA decay, Ribosome biogenesis, rRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33136-MONOMER
ReactomeiR-SCE-3899300 SUMOylation of transcription cofactors
R-SCE-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DBP2 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 2
p68-like protein
Gene namesi
Name:DBP2
Ordered Locus Names:YNL112W
ORF Names:N1945
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL112W
SGDiS000005056 DBP2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi163K → R: Decreases nonsense-mediated mRNA decay. 1 Publication1
Mutagenesisi268E → D: Decreases nonsense-mediated mRNA decay. 1 Publication1
Mutagenesisi300T → A: Decreases nonsense-mediated mRNA decay. 1 Publication1
Mutagenesisi447R → K: Decreases nonsense-mediated mRNA decay. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550001 – 546ATP-dependent RNA helicase DBP2Add BLAST546

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei88PhosphoserineCombined sources1
Modified residuei90PhosphoserineCombined sources1
Cross-linki474Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP24783
PaxDbiP24783
PRIDEiP24783

PTM databases

iPTMnetiP24783

Interactioni

Subunit structurei

Interacts with UPF1. Associates with polysomes.2 Publications

Protein-protein interaction databases

BioGridi35713, 199 interactors
DIPiDIP-2438N
IntActiP24783, 42 interactors
MINTiP24783
STRINGi4932.YNL112W

Structurei

3D structure databases

ProteinModelPortaliP24783
SMRiP24783
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini144 – 319Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST176
Domaini347 – 494Helicase C-terminalPROSITE-ProRule annotationAdd BLAST148

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni505 – 530RNA-binding RGG-boxAdd BLAST26

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi113 – 141Q motifAdd BLAST29
Motifi267 – 270DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00900000140856
HOGENOMiHOG000268804
InParanoidiP24783
KOiK12823
OMAiANMHNGM
OrthoDBiEOG092C1B4A

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

P24783-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYGGRDQQY NKTNYKSRGG DFRGGRNSDR NSYNDRPQGG NYRGGFGGRS
60 70 80 90 100
NYNQPQELIK PNWDEELPKL PTFEKNFYVE HESVRDRSDS EIAQFRKENE
110 120 130 140 150
MTISGHDIPK PITTFDEAGF PDYVLNEVKA EGFDKPTGIQ CQGWPMALSG
160 170 180 190 200
RDMVGIAATG SGKTLSYCLP GIVHINAQPL LAPGDGPIVL VLAPTRELAV
210 220 230 240 250
QIQTECSKFG HSSRIRNTCV YGGVPKSQQI RDLSRGSEIV IATPGRLIDM
260 270 280 290 300
LEIGKTNLKR VTYLVLDEAD RMLDMGFEPQ IRKIVDQIRP DRQTLMWSAT
310 320 330 340 350
WPKEVKQLAA DYLNDPIQVQ VGSLELSASH NITQIVEVVS DFEKRDRLNK
360 370 380 390 400
YLETASQDNE YKTLIFASTK RMCDDITKYL REDGWPALAI HGDKDQRERD
410 420 430 440 450
WVLQEFRNGR SPIMVATDVA ARGIDVKGIN YVINYDMPGN IEDYVHRIGR
460 470 480 490 500
TGRAGATGTA ISFFTEQNKG LGAKLISIMR EANQNIPPEL LKYDRRSYGG
510 520 530 540
GHPRYGGGRG GRGGYGRRGG YGGGRGGYGG NRQRDGGWGN RGRSNY
Length:546
Mass (Da):60,999
Last modified:March 1, 1992 - v1
Checksum:i30FE3D4C7E653120
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti425D → GN in CAA93395 (PubMed:9090055).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52649 Genomic DNA Translation: CAA36874.1
Z71388 Genomic DNA Translation: CAA95991.1
Z69382 Genomic DNA Translation: CAA93395.1
M64991 Genomic DNA No translation available.
BK006947 Genomic DNA Translation: DAA10435.1
PIRiS13757
RefSeqiNP_014287.3, NM_001182950.3

Genome annotation databases

EnsemblFungiiYNL112W; YNL112W; YNL112W
GeneIDi855611
KEGGisce:YNL112W

Similar proteinsi

Entry informationi

Entry nameiDBP2_YEAST
AccessioniPrimary (citable) accession number: P24783
Secondary accession number(s): D6W169, Q05456
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: May 23, 2018
This is version 184 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health