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Protein

ATP-dependent RNA helicase DBP2

Gene

DBP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase involved nonsense-mediated mRNA decay and ribosome biogenesis through rRNA processing.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi157 – 164ATP8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • RNA binding Source: UniProtKB-KW
  • RNA-dependent ATPase activity Source: SGD

GO - Biological processi

  • messenger ribonucleoprotein complex assembly Source: SGD
  • nuclear polyadenylation-dependent mRNA catabolic process Source: SGD
  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: SGD
  • RNA secondary structure unwinding Source: GO_Central
  • rRNA processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Nonsense-mediated mRNA decay, Ribosome biogenesis, rRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33136-MONOMER.
ReactomeiR-SCE-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DBP2 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 2
p68-like protein
Gene namesi
Name:DBP2
Ordered Locus Names:YNL112W
ORF Names:N1945
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL112W.
SGDiS000005056. DBP2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi163K → R: Decreases nonsense-mediated mRNA decay. 1 Publication1
Mutagenesisi268E → D: Decreases nonsense-mediated mRNA decay. 1 Publication1
Mutagenesisi300T → A: Decreases nonsense-mediated mRNA decay. 1 Publication1
Mutagenesisi447R → K: Decreases nonsense-mediated mRNA decay. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550001 – 546ATP-dependent RNA helicase DBP2Add BLAST546

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei88PhosphoserineCombined sources1
Modified residuei90PhosphoserineCombined sources1
Cross-linki474Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP24783.
PRIDEiP24783.

PTM databases

iPTMnetiP24783.

Interactioni

Subunit structurei

Interacts with UPF1. Associates with polysomes.2 Publications

Protein-protein interaction databases

BioGridi35713. 74 interactors.
DIPiDIP-2438N.
IntActiP24783. 41 interactors.
MINTiMINT-636962.

Structurei

3D structure databases

ProteinModelPortaliP24783.
SMRiP24783.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini144 – 319Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST176
Domaini347 – 494Helicase C-terminalPROSITE-ProRule annotationAdd BLAST148

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni505 – 530RNA-binding RGG-boxAdd BLAST26

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi113 – 141Q motifAdd BLAST29
Motifi267 – 270DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00820000126976.
HOGENOMiHOG000268804.
InParanoidiP24783.
KOiK12823.
OMAiVFANRSC.
OrthoDBiEOG092C1B4A.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24783-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYGGRDQQY NKTNYKSRGG DFRGGRNSDR NSYNDRPQGG NYRGGFGGRS
60 70 80 90 100
NYNQPQELIK PNWDEELPKL PTFEKNFYVE HESVRDRSDS EIAQFRKENE
110 120 130 140 150
MTISGHDIPK PITTFDEAGF PDYVLNEVKA EGFDKPTGIQ CQGWPMALSG
160 170 180 190 200
RDMVGIAATG SGKTLSYCLP GIVHINAQPL LAPGDGPIVL VLAPTRELAV
210 220 230 240 250
QIQTECSKFG HSSRIRNTCV YGGVPKSQQI RDLSRGSEIV IATPGRLIDM
260 270 280 290 300
LEIGKTNLKR VTYLVLDEAD RMLDMGFEPQ IRKIVDQIRP DRQTLMWSAT
310 320 330 340 350
WPKEVKQLAA DYLNDPIQVQ VGSLELSASH NITQIVEVVS DFEKRDRLNK
360 370 380 390 400
YLETASQDNE YKTLIFASTK RMCDDITKYL REDGWPALAI HGDKDQRERD
410 420 430 440 450
WVLQEFRNGR SPIMVATDVA ARGIDVKGIN YVINYDMPGN IEDYVHRIGR
460 470 480 490 500
TGRAGATGTA ISFFTEQNKG LGAKLISIMR EANQNIPPEL LKYDRRSYGG
510 520 530 540
GHPRYGGGRG GRGGYGRRGG YGGGRGGYGG NRQRDGGWGN RGRSNY
Length:546
Mass (Da):60,999
Last modified:March 1, 1992 - v1
Checksum:i30FE3D4C7E653120
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti425D → GN in CAA93395 (PubMed:9090055).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52649 Genomic DNA. Translation: CAA36874.1.
Z71388 Genomic DNA. Translation: CAA95991.1.
Z69382 Genomic DNA. Translation: CAA93395.1.
M64991 Genomic DNA. No translation available.
BK006947 Genomic DNA. Translation: DAA10435.1.
PIRiS13757.
RefSeqiNP_014287.3. NM_001182950.3.

Genome annotation databases

EnsemblFungiiYNL112W; YNL112W; YNL112W.
GeneIDi855611.
KEGGisce:YNL112W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52649 Genomic DNA. Translation: CAA36874.1.
Z71388 Genomic DNA. Translation: CAA95991.1.
Z69382 Genomic DNA. Translation: CAA93395.1.
M64991 Genomic DNA. No translation available.
BK006947 Genomic DNA. Translation: DAA10435.1.
PIRiS13757.
RefSeqiNP_014287.3. NM_001182950.3.

3D structure databases

ProteinModelPortaliP24783.
SMRiP24783.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35713. 74 interactors.
DIPiDIP-2438N.
IntActiP24783. 41 interactors.
MINTiMINT-636962.

PTM databases

iPTMnetiP24783.

Proteomic databases

MaxQBiP24783.
PRIDEiP24783.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL112W; YNL112W; YNL112W.
GeneIDi855611.
KEGGisce:YNL112W.

Organism-specific databases

EuPathDBiFungiDB:YNL112W.
SGDiS000005056. DBP2.

Phylogenomic databases

GeneTreeiENSGT00820000126976.
HOGENOMiHOG000268804.
InParanoidiP24783.
KOiK12823.
OMAiVFANRSC.
OrthoDBiEOG092C1B4A.

Enzyme and pathway databases

BioCyciYEAST:G3O-33136-MONOMER.
ReactomeiR-SCE-72165. mRNA Splicing - Minor Pathway.

Miscellaneous databases

PROiP24783.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDBP2_YEAST
AccessioniPrimary (citable) accession number: P24783
Secondary accession number(s): D6W169, Q05456
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 2, 2016
This is version 171 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 33100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.