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Protein

Brachyury protein

Gene

t

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Causes dorsal mesodermal differentiation of animal cap ectoderm when co-expressed with wnt8 and noggin. None of these molecules causes dorsal mesoderm formation when expressed alone. Establishes the left/right axis at early gastrula stage by directly up-regulating mesodermal expression of zic3.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi49 – 217T-boxPROSITE-ProRule annotationAdd BLAST169

GO - Molecular functioni

GO - Biological processi

  • cellular response to fibroblast growth factor stimulus Source: BHF-UCL
  • determination of left/right symmetry Source: UniProtKB
  • embryonic axis specification Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Brachyury protein
Short name:
xBRA
Alternative name(s):
Protein T
Gene namesi
Name:t
Synonyms:bra
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865212. t.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001844171 – 432Brachyury proteinAdd BLAST432

Expressioni

Tissue specificityi

Expressed in presumptive mesodermal cells around the blastopore, and then in the notochord.

Developmental stagei

During gastrula and neurula stages in involuting mesoderm and in the notochord.

Inductioni

By the natural signal and in response to the mesoderm-inducing factors activin A, basic FGF/bFGF, derriere, nodal/nr-1, nodal2/nr-2 and nodal4/nr-4.2 Publications

Interactioni

Subunit structurei

Monomer.

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: BHF-UCL

Structurei

Secondary structure

1432
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi41 – 44Combined sources4
Helixi47 – 56Combined sources10
Beta strandi59 – 61Combined sources3
Beta strandi74 – 78Combined sources5
Beta strandi84 – 97Combined sources14
Beta strandi99 – 103Combined sources5
Beta strandi106 – 112Combined sources7
Beta strandi128 – 130Combined sources3
Helixi131 – 136Combined sources6
Beta strandi145 – 149Combined sources5
Turni152 – 154Combined sources3
Beta strandi160 – 177Combined sources18
Beta strandi180 – 184Combined sources5
Helixi186 – 188Combined sources3
Beta strandi190 – 195Combined sources6
Helixi199 – 208Combined sources10
Helixi210 – 212Combined sources3
Helixi213 – 221Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XBRX-ray2.50A/B39-222[»]
ProteinModelPortaliP24781.
SMRiP24781.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24781.

Family & Domainsi

Sequence similaritiesi

Contains 1 T-box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG004599.
KOiK10172.

Family and domain databases

CDDicd00182. TBOX. 1 hit.
Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR002070. TF_Brachyury.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
[Graphical view]
PRINTSiPR00938. BRACHYURY.
PR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24781-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATESCAKN VQYRVDHLLS AVENELQAGS EKGDPTEKEL KVSLEERDLW
60 70 80 90 100
TRFKELTNEM IVTKNGRRMF PVLKVSMSGL DPNAMYTVLL DFVAADNHRW
110 120 130 140 150
KYVNGEWVPG GKPEPQAPSC VYIHPDSPNF GAHWMKDPVS FSKVKLTNKM
160 170 180 190 200
NGGGQIMLNS LHKYEPRIHI VRVGGTQRMI TSHSFPETQF IAVTAYQNEE
210 220 230 240 250
ITALKIKHNP FAKAFLDAKE RNDYKDILDE GIDSQHSNFS QLGTWLIPNG
260 270 280 290 300
GSLCSPNPHT QFGAPLSLSS PHGCERYSSL RNHRSAPYPS PYTHRNNSPN
310 320 330 340 350
NLADNSSACL SMLQSHDNWS TLQMPAHTGM LPMSHSTGTP PPSSQYPSLW
360 370 380 390 400
SVSNSAITPV SQSGGITNGI SSQYLLGSTP HYSSLSHAVP SPSTGSPLYE
410 420 430
HGAQTEIAEN QYDVTAHSRL SSTWTPVAPP SV
Length:432
Mass (Da):47,599
Last modified:March 1, 1992 - v1
Checksum:i2F91D942D86EE60A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77243 mRNA. Translation: AAA49663.1.
BC072031 mRNA. Translation: AAH72031.1.
PIRiA41056.
RefSeqiNP_001084047.1. NM_001090578.1.
UniGeneiXl.514.

Genome annotation databases

GeneIDi399275.
KEGGixla:399275.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77243 mRNA. Translation: AAA49663.1.
BC072031 mRNA. Translation: AAH72031.1.
PIRiA41056.
RefSeqiNP_001084047.1. NM_001090578.1.
UniGeneiXl.514.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XBRX-ray2.50A/B39-222[»]
ProteinModelPortaliP24781.
SMRiP24781.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399275.
KEGGixla:399275.

Organism-specific databases

CTDi399275.
XenbaseiXB-GENE-865212. t.

Phylogenomic databases

HOVERGENiHBG004599.
KOiK10172.

Miscellaneous databases

EvolutionaryTraceiP24781.

Family and domain databases

CDDicd00182. TBOX. 1 hit.
Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR002070. TF_Brachyury.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
[Graphical view]
PRINTSiPR00938. BRACHYURY.
PR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRAC_XENLA
AccessioniPrimary (citable) accession number: P24781
Secondary accession number(s): Q6IP86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.