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P24772

- B14_VACCW

UniProt

P24772 - B14_VACCW

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Protein

Protein B14

Gene

VACWR196

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Contributes to virulence by binding to the host IKBKB subunit of the IKK complex and preventing host NF-kappa-B activation in response to pro-inflammatory stimuli such as TNF-alpha or IL1B.1 Publication

GO - Biological processi

  1. suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host NF-kappa-B by virus

Names & Taxonomyi

Protein namesi
Recommended name:
Protein B14
Gene namesi
Ordered Locus Names:VACWR196
ORF Names:B14R
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000000344: Genome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi35 – 351Y → E: Disrupts self-association. 1 Publication
Mutagenesisi130 – 1301F → K: Disrupts self-association and NF-kappa-B inhibition by B14. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 149149Protein B14PRO_0000099365Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimers. Interaction with host IKBKB; this interaction inhibits host NF-kappa-B activation.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CHUKO151113EBI-4291651,EBI-81249From a different organism.
IKBKBO149203EBI-4291651,EBI-81266From a different organism.
IkbkgO885222EBI-4291651,EBI-998011From a different organism.

Protein-protein interaction databases

IntActiP24772. 17 interactions.

Structurei

Secondary structure

1
149
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi23 – 253
Helixi26 – 3611
Turni37 – 393
Helixi40 – 423
Helixi49 – 557
Helixi57 – 659
Helixi68 – 747
Helixi80 – 9011
Helixi98 – 11518
Beta strandi118 – 1203
Helixi123 – 13513
Helixi138 – 1447
Helixi145 – 1473

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VVYX-ray2.69A/B/C/D1-149[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24772.

Family & Domainsi

Sequence similaritiesi

Belongs to the orthopoxvirus B14 protein family.Curated

Family and domain databases

InterProiIPR022819. Poxvirus.
IPR011212. Poxvirus_B15/B22/C16.
[Graphical view]
PfamiPF06227. Poxvirus. 1 hit.
[Graphical view]
PIRSFiPIRSF017324. UCP017324. 1 hit.

Sequencei

Sequence statusi: Complete.

P24772 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTANFSTHVF SPQHCGCDRL TSIDDVRQCL TEYIYWSSYA YRNRQCAGQL
60 70 80 90 100
YSTLLSFRDD AELVFIDIRE LVKNMPWDDV KDCAEIIRCY IPDEQKTIRE
110 120 130 140
ISAIIGLCAY AATYWGGEDH PTSNSLNALF VMLEMLNYVD YNIIFRRMN
Length:149
Mass (Da):17,382
Last modified:March 1, 1992 - v1
Checksum:iA6118A960A0B6973
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D11079 Genomic DNA. Translation: BAA01844.1.
M58053 Genomic DNA. Translation: AAA47964.1.
AY243312 Genomic DNA. Translation: AAO89475.1.
PIRiJQ1808.
RefSeqiYP_233078.1. NC_006998.1.

Genome annotation databases

GeneIDi3707573.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D11079 Genomic DNA. Translation: BAA01844.1 .
M58053 Genomic DNA. Translation: AAA47964.1 .
AY243312 Genomic DNA. Translation: AAO89475.1 .
PIRi JQ1808.
RefSeqi YP_233078.1. NC_006998.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2VVY X-ray 2.69 A/B/C/D 1-149 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P24772. 17 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3707573.

Miscellaneous databases

EvolutionaryTracei P24772.

Family and domain databases

InterProi IPR022819. Poxvirus.
IPR011212. Poxvirus_B15/B22/C16.
[Graphical view ]
Pfami PF06227. Poxvirus. 1 hit.
[Graphical view ]
PIRSFi PIRSF017324. UCP017324. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the right inverted terminal repeat."
    Smith G.L., Chan Y.S., Howard S.T.
    J. Gen. Virol. 72:1349-1376(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Vaccinia virus homologues of the Shope fibroma virus inverted terminal repeat proteins and a discontinuous ORF related to the tumor necrosis factor receptor family."
    Howard S.T., Chan Y.S., Smith G.L.
    Virology 180:633-647(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
    Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Inhibition of IkappaB kinase by vaccinia virus virulence factor B14."
    Chen R.A., Ryzhakov G., Cooray S., Randow F., Smith G.L.
    PLoS Pathog. 4:E22-E22(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HOST IKBKB.
  5. "A poxvirus Bcl-2-like gene family involved in regulation of host immune response: sequence similarity and evolutionary history."
    Gonzalez J.M., Esteban M.
    Virol. J. 7:59-59(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIMILARITY.
  6. "Mapping the I{kappa}B Kinase {beta} (IKK{beta})-binding interface of the B14 protein, a vaccinia virus inhibitor of IKK{beta}-mediated activation of nuclear factor {kappa}B."
    Benfield C.T., Mansur D.S., McCoy L.E., Ferguson B.J., Bahar M.W., Oldring A.P., Grimes J.M., Stuart D.I., Graham S.C., Smith G.L.
    J. Biol. Chem. 286:20727-20735(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF TYR-35 AND PHE-130.
  7. "Vaccinia virus proteins A52 and B14 Share a Bcl-2-like fold but have evolved to inhibit NF-kappaB rather than apoptosis."
    Graham S.C., Bahar M.W., Cooray S., Chen R.A., Whalen D.M., Abrescia N.G., Alderton D., Owens R.J., Stuart D.I., Smith G.L., Grimes J.M.
    PLoS Pathog. 4:E1000128-E1000128(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.69 ANGSTROMS).

Entry informationi

Entry nameiB14_VACCW
AccessioniPrimary (citable) accession number: P24772
Secondary accession number(s): Q76ZK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: October 29, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3