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P24772 (B14_VACCW) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein B14
Gene names
Ordered Locus Names:VACWR196
ORF Names:B14R
OrganismVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) [Reference proteome]
Taxonomic identifier10254 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostBos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length149 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Contributes to virulence by binding to the host IKBKB subunit of the IKK complex and preventing host NF-kappa-B activation in response to pro-inflammatory stimuli such as TNF-alpha or IL1B. Ref.6

Subunit structure

Homodimers. Interaction with host IKBKB; this interaction inhibits host NF-kappa-B activation. Ref.6

Sequence similarities

Belongs to the orthopoxvirus B14 protein family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CHUKO151113EBI-4291651,EBI-81249From a different organism.
IKBKBO149203EBI-4291651,EBI-81266From a different organism.
IkbkgO885222EBI-4291651,EBI-998011From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 149149Protein B14
PRO_0000099365

Experimental info

Mutagenesis351Y → E: Disrupts self-association. Ref.6
Mutagenesis1301F → K: Disrupts self-association and NF-kappa-B inhibition by B14. Ref.6

Secondary structure

....................... 149
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P24772 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: A6118A960A0B6973

FASTA14917,382
        10         20         30         40         50         60 
MTANFSTHVF SPQHCGCDRL TSIDDVRQCL TEYIYWSSYA YRNRQCAGQL YSTLLSFRDD 

        70         80         90        100        110        120 
AELVFIDIRE LVKNMPWDDV KDCAEIIRCY IPDEQKTIRE ISAIIGLCAY AATYWGGEDH 

       130        140 
PTSNSLNALF VMLEMLNYVD YNIIFRRMN 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the right inverted terminal repeat."
Smith G.L., Chan Y.S., Howard S.T.
J. Gen. Virol. 72:1349-1376(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Vaccinia virus homologues of the Shope fibroma virus inverted terminal repeat proteins and a discontinuous ORF related to the tumor necrosis factor receptor family."
Howard S.T., Chan Y.S., Smith G.L.
Virology 180:633-647(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Inhibition of IkappaB kinase by vaccinia virus virulence factor B14."
Chen R.A., Ryzhakov G., Cooray S., Randow F., Smith G.L.
PLoS Pathog. 4:E22-E22(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HOST IKBKB.
[5]"A poxvirus Bcl-2-like gene family involved in regulation of host immune response: sequence similarity and evolutionary history."
Gonzalez J.M., Esteban M.
Virol. J. 7:59-59(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SIMILARITY.
[6]"Mapping the I{kappa}B Kinase {beta} (IKK{beta})-binding interface of the B14 protein, a vaccinia virus inhibitor of IKK{beta}-mediated activation of nuclear factor {kappa}B."
Benfield C.T., Mansur D.S., McCoy L.E., Ferguson B.J., Bahar M.W., Oldring A.P., Grimes J.M., Stuart D.I., Graham S.C., Smith G.L.
J. Biol. Chem. 286:20727-20735(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF TYR-35 AND PHE-130.
[7]"Vaccinia virus proteins A52 and B14 Share a Bcl-2-like fold but have evolved to inhibit NF-kappaB rather than apoptosis."
Graham S.C., Bahar M.W., Cooray S., Chen R.A., Whalen D.M., Abrescia N.G., Alderton D., Owens R.J., Stuart D.I., Smith G.L., Grimes J.M.
PLoS Pathog. 4:E1000128-E1000128(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.69 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D11079 Genomic DNA. Translation: BAA01844.1.
M58053 Genomic DNA. Translation: AAA47964.1.
AY243312 Genomic DNA. Translation: AAO89475.1.
PIRJQ1808.
RefSeqYP_233078.1. NC_006998.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2VVYX-ray2.69A/B/C/D1-149[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP24772. 17 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3707573.

Family and domain databases

InterProIPR022819. Poxvirus.
IPR011212. Poxvirus_B15/B22/C16.
[Graphical view]
PfamPF06227. Poxvirus. 1 hit.
[Graphical view]
PIRSFPIRSF017324. UCP017324. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP24772.

Entry information

Entry nameB14_VACCW
AccessionPrimary (citable) accession number: P24772
Secondary accession number(s): Q76ZK9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 11, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references