P24772 (B14_VACCW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein B14

Gene names

Ordered Locus Names:	VACWR196
ORF Names:	B14R

Organism

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) [Reference proteome]

Taxonomic identifier

10254 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Contributes to virulence by binding to the host IKBKB subunit of the IKK complex and preventing host NF-kappa-B activation in response to pro-inflammatory stimuli such as TNF-alpha or IL1B. Ref.6
Subunit structure	Homodimers. Interaction with host IKBKB; this interaction inhibits host NF-kappa-B activation. Ref.6
Sequence similarities	Belongs to the orthopoxvirus B14 protein family.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host NF-kappa-B by virus
Developmental stage	Early protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	suppression by virus of host NF-kappaB transcription factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CHUK	O15111	3	EBI-4291651,EBI-81249	From a different organism.
IKBKB	O14920	3	EBI-4291651,EBI-81266	From a different organism.
Ikbkg	O88522	2	EBI-4291651,EBI-998011	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 149

149

Protein B14

PRO_0000099365

Experimental info

Mutagenesis

35

1

Y → E: Disrupts self-association. Ref.6

Mutagenesis

130

1

F → K: Disrupts self-association and NF-kappa-B inhibition by B14. Ref.6

Secondary structure

1

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149

Helix Strand Turn

Details...

Helix

23 – 25

3

Helix

26 – 36

11

Turn

37 – 39

3

Helix

40 – 42

3

Helix

49 – 55

7

Helix

57 – 65

9

Helix

68 – 74

7

Helix

80 – 90

11

Helix

98 – 115

18

Beta strand

118 – 120

3

Helix

123 – 135

13

Helix

138 – 144

7

Helix

145 – 147

3

Sequences

	Sequence		Length	Mass (Da)
	P24772 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: A6118A960A0B6973	FASTA	149	17,382
	10 20 30 40 50 60 MTANFSTHVF SPQHCGCDRL TSIDDVRQCL TEYIYWSSYA YRNRQCAGQL YSTLLSFRDD 70 80 90 100 110 120 AELVFIDIRE LVKNMPWDDV KDCAEIIRCY IPDEQKTIRE ISAIIGLCAY AATYWGGEDH 130 140 PTSNSLNALF VMLEMLNYVD YNIIFRRMN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the right inverted terminal repeat." Smith G.L., Chan Y.S., Howard S.T. J. Gen. Virol. 72:1349-1376(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Vaccinia virus homologues of the Shope fibroma virus inverted terminal repeat proteins and a discontinuous ORF related to the tumor necrosis factor receptor family." Howard S.T., Chan Y.S., Smith G.L. Virology 180:633-647(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb." Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Inhibition of IkappaB kinase by vaccinia virus virulence factor B14." Chen R.A., Ryzhakov G., Cooray S., Randow F., Smith G.L. PLoS Pathog. 4:E22-E22(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HOST IKBKB.
[5]	"A poxvirus Bcl-2-like gene family involved in regulation of host immune response: sequence similarity and evolutionary history." Gonzalez J.M., Esteban M. Virol. J. 7:59-59(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SIMILARITY.
[6]	"Mapping the I{kappa}B Kinase {beta} (IKK{beta})-binding interface of the B14 protein, a vaccinia virus inhibitor of IKK{beta}-mediated activation of nuclear factor {kappa}B." Benfield C.T., Mansur D.S., McCoy L.E., Ferguson B.J., Bahar M.W., Oldring A.P., Grimes J.M., Stuart D.I., Graham S.C., Smith G.L. J. Biol. Chem. 286:20727-20735(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF TYR-35 AND PHE-130.
[7]	"Vaccinia virus proteins A52 and B14 Share a Bcl-2-like fold but have evolved to inhibit NF-kappaB rather than apoptosis." Graham S.C., Bahar M.W., Cooray S., Chen R.A., Whalen D.M., Abrescia N.G., Alderton D., Owens R.J., Stuart D.I., Smith G.L., Grimes J.M. PLoS Pathog. 4:E1000128-E1000128(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.69 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D11079 Genomic DNA. Translation: BAA01844.1.
M58053 Genomic DNA. Translation: AAA47964.1.
AY243312 Genomic DNA. Translation: AAO89475.1.

PIR

JQ1808.

RefSeq

YP_233078.1. NC_006998.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VVY	X-ray	2.69	A/B/C/D	1-149	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P24772. 17 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3707573.

Phylogenomic databases

ProtClustDB

CLSP2509856.

Family and domain databases

InterPro

IPR022819. Poxvirus.
IPR011212. Poxvirus_B15/B22/C16.
[Graphical view]

Pfam

PF06227. Poxvirus. 1 hit.
[Graphical view]

PIRSF

PIRSF017324. UCP017324. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P24772.

Entry information

Entry name

B14_VACCW

Accession

Primary (citable) accession number: P24772
Secondary accession number(s): Q76ZK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program