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P24772

- B14_VACCW

UniProt

P24772 - B14_VACCW

Protein

Protein B14

Gene

VACWR196

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Contributes to virulence by binding to the host IKBKB subunit of the IKK complex and preventing host NF-kappa-B activation in response to pro-inflammatory stimuli such as TNF-alpha or IL1B.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host NF-kappa-B by virus

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein B14
    Gene namesi
    Ordered Locus Names:VACWR196
    ORF Names:B14R
    OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
    Taxonomic identifieri10254 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    ProteomesiUP000000344: Genome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi35 – 351Y → E: Disrupts self-association. 1 Publication
    Mutagenesisi130 – 1301F → K: Disrupts self-association and NF-kappa-B inhibition by B14. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 149149Protein B14PRO_0000099365Add
    BLAST

    Expressioni

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Homodimers. Interaction with host IKBKB; this interaction inhibits host NF-kappa-B activation.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CHUKO151113EBI-4291651,EBI-81249From a different organism.
    IKBKBO149203EBI-4291651,EBI-81266From a different organism.
    IkbkgO885222EBI-4291651,EBI-998011From a different organism.

    Protein-protein interaction databases

    IntActiP24772. 17 interactions.

    Structurei

    Secondary structure

    1
    149
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi23 – 253
    Helixi26 – 3611
    Turni37 – 393
    Helixi40 – 423
    Helixi49 – 557
    Helixi57 – 659
    Helixi68 – 747
    Helixi80 – 9011
    Helixi98 – 11518
    Beta strandi118 – 1203
    Helixi123 – 13513
    Helixi138 – 1447
    Helixi145 – 1473

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2VVYX-ray2.69A/B/C/D1-149[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP24772.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the orthopoxvirus B14 protein family.Curated

    Family and domain databases

    InterProiIPR022819. Poxvirus.
    IPR011212. Poxvirus_B15/B22/C16.
    [Graphical view]
    PfamiPF06227. Poxvirus. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017324. UCP017324. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P24772-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTANFSTHVF SPQHCGCDRL TSIDDVRQCL TEYIYWSSYA YRNRQCAGQL    50
    YSTLLSFRDD AELVFIDIRE LVKNMPWDDV KDCAEIIRCY IPDEQKTIRE 100
    ISAIIGLCAY AATYWGGEDH PTSNSLNALF VMLEMLNYVD YNIIFRRMN 149
    Length:149
    Mass (Da):17,382
    Last modified:March 1, 1992 - v1
    Checksum:iA6118A960A0B6973
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11079 Genomic DNA. Translation: BAA01844.1.
    M58053 Genomic DNA. Translation: AAA47964.1.
    AY243312 Genomic DNA. Translation: AAO89475.1.
    PIRiJQ1808.
    RefSeqiYP_233078.1. NC_006998.1.

    Genome annotation databases

    GeneIDi3707573.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11079 Genomic DNA. Translation: BAA01844.1 .
    M58053 Genomic DNA. Translation: AAA47964.1 .
    AY243312 Genomic DNA. Translation: AAO89475.1 .
    PIRi JQ1808.
    RefSeqi YP_233078.1. NC_006998.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2VVY X-ray 2.69 A/B/C/D 1-149 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P24772. 17 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3707573.

    Miscellaneous databases

    EvolutionaryTracei P24772.

    Family and domain databases

    InterProi IPR022819. Poxvirus.
    IPR011212. Poxvirus_B15/B22/C16.
    [Graphical view ]
    Pfami PF06227. Poxvirus. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017324. UCP017324. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the right inverted terminal repeat."
      Smith G.L., Chan Y.S., Howard S.T.
      J. Gen. Virol. 72:1349-1376(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Vaccinia virus homologues of the Shope fibroma virus inverted terminal repeat proteins and a discontinuous ORF related to the tumor necrosis factor receptor family."
      Howard S.T., Chan Y.S., Smith G.L.
      Virology 180:633-647(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
      Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Inhibition of IkappaB kinase by vaccinia virus virulence factor B14."
      Chen R.A., Ryzhakov G., Cooray S., Randow F., Smith G.L.
      PLoS Pathog. 4:E22-E22(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HOST IKBKB.
    5. "A poxvirus Bcl-2-like gene family involved in regulation of host immune response: sequence similarity and evolutionary history."
      Gonzalez J.M., Esteban M.
      Virol. J. 7:59-59(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SIMILARITY.
    6. "Mapping the I{kappa}B Kinase {beta} (IKK{beta})-binding interface of the B14 protein, a vaccinia virus inhibitor of IKK{beta}-mediated activation of nuclear factor {kappa}B."
      Benfield C.T., Mansur D.S., McCoy L.E., Ferguson B.J., Bahar M.W., Oldring A.P., Grimes J.M., Stuart D.I., Graham S.C., Smith G.L.
      J. Biol. Chem. 286:20727-20735(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF TYR-35 AND PHE-130.
    7. "Vaccinia virus proteins A52 and B14 Share a Bcl-2-like fold but have evolved to inhibit NF-kappaB rather than apoptosis."
      Graham S.C., Bahar M.W., Cooray S., Chen R.A., Whalen D.M., Abrescia N.G., Alderton D., Owens R.J., Stuart D.I., Smith G.L., Grimes J.M.
      PLoS Pathog. 4:E1000128-E1000128(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.69 ANGSTROMS).

    Entry informationi

    Entry nameiB14_VACCW
    AccessioniPrimary (citable) accession number: P24772
    Secondary accession number(s): Q76ZK9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3