Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein B14

Gene

VACWR196

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to virulence by binding to the host IKBKB subunit of the IKK complex and preventing host NF-kappa-B activation in response to pro-inflammatory stimuli such as TNF-alpha or IL1B.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host NF-kappa-B by virus

Names & Taxonomyi

Protein namesi
Recommended name:
Protein B14
Gene namesi
Ordered Locus Names:VACWR196
ORF Names:B14R
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Proteomesi
  • UP000000344 Componenti: Genome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi35Y → E: Disrupts self-association. 1 Publication1
Mutagenesisi130F → K: Disrupts self-association and NF-kappa-B inhibition by B14. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000993651 – 149Protein B14Add BLAST149

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimers. Interaction with host IKBKB; this interaction inhibits host NF-kappa-B activation.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CHUKO151113EBI-4291651,EBI-81249From a different organism.
IKBKBO149203EBI-4291651,EBI-81266From a different organism.
IkbkgO885222EBI-4291651,EBI-998011From a different organism.

Protein-protein interaction databases

IntActiP24772. 17 interactors.

Structurei

Secondary structure

1149
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 25Combined sources3
Helixi26 – 36Combined sources11
Turni37 – 39Combined sources3
Helixi40 – 42Combined sources3
Helixi49 – 55Combined sources7
Helixi57 – 65Combined sources9
Helixi68 – 74Combined sources7
Helixi80 – 90Combined sources11
Helixi98 – 115Combined sources18
Beta strandi118 – 120Combined sources3
Helixi123 – 135Combined sources13
Helixi138 – 144Combined sources7
Helixi145 – 147Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VVYX-ray2.69A/B/C/D1-149[»]
SMRiP24772.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24772.

Family & Domainsi

Sequence similaritiesi

Belongs to the orthopoxvirus B14 protein family.Curated

Family and domain databases

InterProiIPR022819. Poxvirus.
IPR011212. Poxvirus_B15/B22/C16.
[Graphical view]
PfamiPF06227. Poxvirus. 1 hit.
[Graphical view]
PIRSFiPIRSF017324. UCP017324. 1 hit.

Sequencei

Sequence statusi: Complete.

P24772-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTANFSTHVF SPQHCGCDRL TSIDDVRQCL TEYIYWSSYA YRNRQCAGQL
60 70 80 90 100
YSTLLSFRDD AELVFIDIRE LVKNMPWDDV KDCAEIIRCY IPDEQKTIRE
110 120 130 140
ISAIIGLCAY AATYWGGEDH PTSNSLNALF VMLEMLNYVD YNIIFRRMN
Length:149
Mass (Da):17,382
Last modified:March 1, 1992 - v1
Checksum:iA6118A960A0B6973
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11079 Genomic DNA. Translation: BAA01844.1.
M58053 Genomic DNA. Translation: AAA47964.1.
AY243312 Genomic DNA. Translation: AAO89475.1.
PIRiJQ1808.
RefSeqiYP_233078.1. NC_006998.1.

Genome annotation databases

GeneIDi3707573.
KEGGivg:3707573.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11079 Genomic DNA. Translation: BAA01844.1.
M58053 Genomic DNA. Translation: AAA47964.1.
AY243312 Genomic DNA. Translation: AAO89475.1.
PIRiJQ1808.
RefSeqiYP_233078.1. NC_006998.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VVYX-ray2.69A/B/C/D1-149[»]
SMRiP24772.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24772. 17 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3707573.
KEGGivg:3707573.

Miscellaneous databases

EvolutionaryTraceiP24772.

Family and domain databases

InterProiIPR022819. Poxvirus.
IPR011212. Poxvirus_B15/B22/C16.
[Graphical view]
PfamiPF06227. Poxvirus. 1 hit.
[Graphical view]
PIRSFiPIRSF017324. UCP017324. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB14_VACCW
AccessioniPrimary (citable) accession number: P24772
Secondary accession number(s): Q76ZK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 2, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.