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P24748 (G3P_CITFR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glyceraldehyde-3-phosphate dehydrogenase
Short name=GAPDH
EC=1.2.1.12
Gene names
Name:gap
OrganismCitrobacter freundii
Taxonomic identifier546 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length294 AA.
Sequence statusFragment.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNAD binding

Inferred from electronic annotation. Source: InterPro

NADP binding

Inferred from electronic annotation. Source: InterPro

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›294›294Glyceraldehyde-3-phosphate dehydrogenase
PRO_0000145644

Regions

Region134 – 1363Glyceraldehyde 3-phosphate binding By similarity
Region194 – 1952Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site1351Nucleophile By similarity
Binding site191NAD By similarity
Binding site631NAD; via carbonyl oxygen By similarity
Binding site1651Glyceraldehyde 3-phosphate By similarity
Binding site2171Glyceraldehyde 3-phosphate By similarity
Site1621Activates thiol group during catalysis By similarity

Experimental info

Non-terminal residue11
Non-terminal residue2941

Sequences

Sequence LengthMass (Da)Tools
P24748 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: A112B777854B146A

FASTA29431,478
        10         20         30         40         50         60 
IVFRAAQERS DIEIVAINDL LDADYMAYML KYDSTHGRFN GTVEVKDGHL IVNGKKIRVT 

        70         80         90        100        110        120 
AERDPANLKW DEVGVDVVAE ATGLFLTDET ARKHITAGAK KVVLTGPSKD NTPMFVKGAN 

       130        140        150        160        170        180 
FDKYEGQDIV SNASCTTNCL APLAKVINDN FGIIEGLMTT VHATTATQKT VDGPSHKDWR 

       190        200        210        220        230        240 
GGRGASQNII PSSTGAAKAV GKVLPELNGK LTGMAFRVPT PNVSVVDLTV RLEKAASYEE 

       250        260        270        280        290 
IKKAIKAASE GPMKGVLGYT EDDVVSTDFN GEVCTSVFDA KAGIALNDNF VKLV

« Hide

References

[1]"Molecular and evolutionary relationships among enteric bacteria."
Lawrence J.G., Ochman H., Hartl D.L.
J. Gen. Microbiol. 137:1911-1921(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: OS60.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M63370 Genomic DNA. Translation: AAA23094.1.
PIRI40701.

3D structure databases

ProteinModelPortalP24748.
SMRP24748. Positions 1-294.
ModBaseSearch...

Proteomic databases

PRIDEP24748.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00109; UER00184.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR10836. PTHR10836. 1 hit.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000149. GAP_DH. 1 hit.
PRINTSPR00078. G3PDHDRGNASE.
SMARTSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01534. GAPDH-I. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG3P_CITFR
AccessionPrimary (citable) accession number: P24748
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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