P24735 (AMPC_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase
EC=3.5.2.6

Alternative name(s):
Cephalosporinase

Gene names

Name:	ampC
Ordered Locus Names:	PA4110

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the class-C beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Periplasm
Domain	Signal
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

26

Ref.3

Chain

27 – 397

371

Beta-lactamase

PRO_0000016963

Regions

Region

342 – 344

3

Substrate binding By similarity

Sites

Active site

90

1

Acyl-ester intermediate By similarity

Active site

177

1

Proton acceptor By similarity

Experimental info

Sequence conflict

397

1

R → A in CAA38522. Ref.1

Secondary structure

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397

Helix Strand Turn

Details...

Helix

29 – 48

20

Beta strand

52 – 60

9

Beta strand

63 – 72

10

Turn

74 – 76

3

Beta strand

85 – 87

3

Helix

89 – 91

3

Helix

92 – 105

14

Helix

115 – 118

4

Helix

120 – 122

3

Helix

126 – 129

4

Helix

132 – 136

5

Helix

155 – 164

10

Beta strand

173 – 175

3

Helix

179 – 192

14

Helix

197 – 203

7

Helix

205 – 208

4

Beta strand

212 – 217

6

Helix

220 – 225

6

Helix

232 – 234

3

Helix

245 – 249

5

Beta strand

252 – 254

3

Helix

255 – 266

12

Helix

268 – 270

3

Helix

273 – 280

8

Beta strand

283 – 289

7

Beta strand

292 – 294

3

Beta strand

299 – 304

6

Helix

307 – 313

7

Helix

316 – 320

5

Beta strand

326 – 333

8

Beta strand

336 – 345

10

Beta strand

350 – 356

7

Helix

357 – 359

3

Beta strand

361 – 369

9

Helix

373 – 388

16

Sequences

	Sequence		Length	Mass (Da)
	P24735 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: C6341A53594BC261	FASTA	397	43,401
	10 20 30 40 50 60 MRDTRFPCLC GIAASTLLFA TTPAIAGEAP ADRLKALVDA AVQPVMKAND IPGLAVAISL 70 80 90 100 110 120 KGEPHYFSYG LASKEDGRRV TPETLFEIGS VSKTFTATLA GYALTQDKMR LDDRASQHWP 130 140 150 160 170 180 ALQGSRFDGI SLLDLATYTA GGLPLQFPDS VQKDQAQIRD YYRQWQPTYA PGSQRLYSNP 190 200 210 220 230 240 SIGLFGYLAA RSLGQPFERL MEQQVFPALG LEQTHLDVPE AALAQYAQGY GKDDRPLRVG 250 260 270 280 290 300 PGPLDAEGYG VKTSAADLLR FVDANLHPER LDRPWAQALD ATHRGYYKVG DMTQGLGWEA 310 320 330 340 350 360 YDWPISLKRL QAGNSTPMAL QPHRIARLPA PQALEGQRLL NKTGSTNGFG AYVAFVPGRD 370 380 390 LGLVILANRN YPNAERVKIA YAILSGLEQQ GKVPLKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase." Lodge J.M., Minchin S.D., Piddock L.J.V., Busby S.J.W. Biochem. J. 272:627-631(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]	"Two-dimensional polyacrylamide gel electrophoresis isolation and microsequencing of Pseudomonas aeruginosa proteins." Michea-Hamzehpour M., Sanchez J.-C., Epp S.F., Paquet N., Hughes G.J., Hochstrasser D.F., Pechere J.-C. Enzyme Protein 47:1-8(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-41.
[4]	"Investigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampC beta-lactamase promoter." Lodge J.M., Busby S.J.W., Piddock L.J.V. FEMS Microbiol. Lett. 111:315-320(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X54719 Genomic DNA. Translation: CAA38522.1.
AE004091 Genomic DNA. Translation: AAG07497.1.
X67095 Genomic DNA. Translation: CAA47469.1.

PIR

F83132.
S13408.

RefSeq

NP_252799.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WZX	X-ray	1.40	A	27-397	[»]
2WZZ	X-ray	1.57	A	27-397	[»]
3S1Y	X-ray	1.40	A	27-396	[»]
3S22	X-ray	1.65	A	27-396	[»]

ProteinModelPortal

P24735.

SMR

P24735. Positions 28-389.

ModBase

Search...

Protein-protein interaction databases

STRING

208964.PA4110.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

878149.

KEGG

pae:PA4110.

PATRIC

19842900. VBIPseAer58763_4306.

Organism-specific databases

PseudoCAP

PA4110.

Phylogenomic databases

eggNOG

COG1680.

HOGENOM

HOG000267102.

KO

K01467.

OMA

QRLYSNP.

ProtClustDB

PRK11289.

Enzyme and pathway databases

SABIO-RK

P24735.

Family and domain databases

Gene3D

3.40.710.10. 1 hit.

InterPro

IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]

Pfam

PF00144. Beta-lactamase. 1 hit.
[Graphical view]

SUPFAM

SSF56601. PBP_transp_fold. 1 hit.

PROSITE

PS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P24735.

ChEMBL

CHEMBL5031.

EvolutionaryTrace

P24735.

Entry information

Entry name

AMPC_PSEAE

Accession

Primary (citable) accession number: P24735

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	December 8, 2000
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program