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P24735 (AMPC_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase

EC=3.5.2.6
Alternative name(s):
Cephalosporinase
Gene names
Name:ampC
Ordered Locus Names:PA4110
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the class-C beta-lactamase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Ref.3
Chain27 – 397371Beta-lactamase
PRO_0000016963

Regions

Region342 – 3443Substrate binding By similarity

Sites

Active site901Acyl-ester intermediate By similarity
Active site1771Proton acceptor By similarity

Experimental info

Sequence conflict3971R → A in CAA38522. Ref.1

Secondary structure

.................................................................... 397
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P24735 [UniParc].

Last modified December 8, 2000. Version 2.
Checksum: C6341A53594BC261

FASTA39743,401
        10         20         30         40         50         60 
MRDTRFPCLC GIAASTLLFA TTPAIAGEAP ADRLKALVDA AVQPVMKAND IPGLAVAISL 

        70         80         90        100        110        120 
KGEPHYFSYG LASKEDGRRV TPETLFEIGS VSKTFTATLA GYALTQDKMR LDDRASQHWP 

       130        140        150        160        170        180 
ALQGSRFDGI SLLDLATYTA GGLPLQFPDS VQKDQAQIRD YYRQWQPTYA PGSQRLYSNP 

       190        200        210        220        230        240 
SIGLFGYLAA RSLGQPFERL MEQQVFPALG LEQTHLDVPE AALAQYAQGY GKDDRPLRVG 

       250        260        270        280        290        300 
PGPLDAEGYG VKTSAADLLR FVDANLHPER LDRPWAQALD ATHRGYYKVG DMTQGLGWEA 

       310        320        330        340        350        360 
YDWPISLKRL QAGNSTPMAL QPHRIARLPA PQALEGQRLL NKTGSTNGFG AYVAFVPGRD 

       370        380        390 
LGLVILANRN YPNAERVKIA YAILSGLEQQ GKVPLKR 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase."
Lodge J.M., Minchin S.D., Piddock L.J.V., Busby S.J.W.
Biochem. J. 272:627-631(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]"Two-dimensional polyacrylamide gel electrophoresis isolation and microsequencing of Pseudomonas aeruginosa proteins."
Michea-Hamzehpour M., Sanchez J.-C., Epp S.F., Paquet N., Hughes G.J., Hochstrasser D.F., Pechere J.-C.
Enzyme Protein 47:1-8(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 27-41.
[4]"Investigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampC beta-lactamase promoter."
Lodge J.M., Busby S.J.W., Piddock L.J.V.
FEMS Microbiol. Lett. 111:315-320(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3.
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54719 Genomic DNA. Translation: CAA38522.1.
AE004091 Genomic DNA. Translation: AAG07497.1.
X67095 Genomic DNA. Translation: CAA47469.1.
PIRF83132.
S13408.
RefSeqNP_252799.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2WZXX-ray1.40A27-397[»]
2WZZX-ray1.57A27-397[»]
3S1YX-ray1.40A27-396[»]
3S22X-ray1.65A27-396[»]
4GZBX-ray1.79A27-397[»]
4HEFX-ray1.86A29-388[»]
4NK3X-ray1.90A27-397[»]
ProteinModelPortalP24735.
SMRP24735. Positions 28-389.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA4110.

Chemistry

BindingDBP24735.
ChEMBLCHEMBL5031.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID878149.
KEGGpae:PA4110.
PATRIC19842900. VBIPseAer58763_4306.

Organism-specific databases

PseudoCAPPA4110.

Phylogenomic databases

eggNOGCOG1680.
HOGENOMHOG000267102.
KOK01467.
OMAQRLYSNP.
OrthoDBEOG60SCH1.
ProtClustDBPRK11289.

Enzyme and pathway databases

SABIO-RKP24735.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP24735.
PROP24735.

Entry information

Entry nameAMPC_PSEAE
AccessionPrimary (citable) accession number: P24735
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: December 8, 2000
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references