Reviewed, UniProtKB/Swiss-Prot P24735 (AMPC_PSEAE)

Names and origin

Protein names

Recommended name:
    Beta-lactamase
    EC=3.5.2.6
Alternative name(s):
    Cephalosporinase

Gene names

Name:	ampC
Ordered Locus Names:	PA4110

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the class-C beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Periplasm
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Ref.3
	Chain	27 – 397	371	Beta-lactamase		PRO_0000016963
Regions
	Region	342 – 344	3	Substrate binding By similarity
Sites
	Active site	90	1	Acyl-ester intermediate By similarity
	Active site	177	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	397	1	R → A in CAA38522. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24735-1 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: C6341A53594BC261	FASTA	397	43,401
	10 20 30 40 50 60 MRDTRFPCLC GIAASTLLFA TTPAIAGEAP ADRLKALVDA AVQPVMKAND IPGLAVAISL 70 80 90 100 110 120 KGEPHYFSYG LASKEDGRRV TPETLFEIGS VSKTFTATLA GYALTQDKMR LDDRASQHWP 130 140 150 160 170 180 ALQGSRFDGI SLLDLATYTA GGLPLQFPDS VQKDQAQIRD YYRQWQPTYA PGSQRLYSNP 190 200 210 220 230 240 SIGLFGYLAA RSLGQPFERL MEQQVFPALG LEQTHLDVPE AALAQYAQGY GKDDRPLRVG 250 260 270 280 290 300 PGPLDAEGYG VKTSAADLLR FVDANLHPER LDRPWAQALD ATHRGYYKVG DMTQGLGWEA 310 320 330 340 350 360 YDWPISLKRL QAGNSTPMAL QPHRIARLPA PQALEGQRLL NKTGSTNGFG AYVAFVPGRD 370 380 390 LGLVILANRN YPNAERVKIA YAILSGLEQQ GKVPLKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase." Lodge J.M., Minchin S.D., Piddock L.J.V., Busby S.J.W. Biochem. J. 272:627-631(1990) [PubMed: 2125210] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]	"Two-dimensional polyacrylamide gel electrophoresis isolation and microsequencing of Pseudomonas aeruginosa proteins." Michea-Hamzehpour M., Sanchez J.-C., Epp S.F., Paquet N., Hughes G.J., Hochstrasser D.F., Pechere J.-C. Enzyme Protein 47:1-8(1993) [PubMed: 8012497] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-41.
[4]	"Investigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampC beta-lactamase promoter." Lodge J.M., Busby S.J.W., Piddock L.J.V. FEMS Microbiol. Lett. 111:315-320(1993) [PubMed: 8405939] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54719 Genomic DNA. Translation: CAA38522.1. AE004091 Genomic DNA. Translation: AAG07497.1. X67095 Genomic DNA. Translation: CAA47469.1.
PIR	F83132. S13408.
RefSeq	NP_252799.1.
3D structure databases
HSSP	HSSP built from PDB template 1GA9 based on UniProtKB P00811.
ModBase	Search...
Protein family/group databases
MEROPS	S12.006.
Genome annotation databases
GeneID	878149.
GenomeReviews	Gene locus PA4110 in contig AE004091_GR.
KEGG	pae:PA4110.
Organism-specific databases
PseudoCAP	PA4110.
CMR	Search...
Phylogenomic databases
HOGENOM	P24735.
OMA	YNYGVAS.
Enzyme and pathway databases
BioCyc	PAER208964:PA4110-MON.
BRENDA	3.5.2.6. 354.
Family and domain databases
InterPro	IPR001586. Beta_lactam_C_AS. IPR001466. Beta_lactamase-related. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PROSITE	PS00336. BETA_LACTAMASE_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPC_PSEAE

Accession

Primary (citable) accession number: P24735

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	December 8, 2000
Last modified:	November 3, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)