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P24735

- AMPC_PSEAE

UniProt

P24735 - AMPC_PSEAE

Protein

Beta-lactamase

Gene

ampC

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (08 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei90 – 901Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei177 – 1771Proton acceptorBy similarity

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. antibiotic catabolic process Source: InterPro
    2. regulation of beta-lactamase activity Source: PseudoCAP
    3. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Enzyme and pathway databases

    SABIO-RKP24735.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase (EC:3.5.2.6)
    Alternative name(s):
    Cephalosporinase
    Gene namesi
    Name:ampC
    Ordered Locus Names:PA4110
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA4110.

    Subcellular locationi

    Periplasm By similarity

    GO - Cellular componenti

    1. outer membrane-bounded periplasmic space Source: InterPro

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 26261 PublicationAdd
    BLAST
    Chaini27 – 397371Beta-lactamasePRO_0000016963Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi208964.PA4110.

    Structurei

    Secondary structure

    1
    397
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi29 – 4820
    Beta strandi52 – 609
    Beta strandi63 – 7210
    Turni74 – 763
    Beta strandi85 – 873
    Helixi89 – 913
    Helixi92 – 10514
    Helixi115 – 1184
    Helixi120 – 1223
    Helixi126 – 1294
    Helixi132 – 1365
    Helixi155 – 16410
    Beta strandi173 – 1753
    Helixi179 – 19214
    Helixi197 – 2037
    Helixi205 – 2084
    Beta strandi212 – 2176
    Helixi220 – 2256
    Helixi232 – 2343
    Helixi245 – 2495
    Beta strandi252 – 2543
    Helixi255 – 26612
    Helixi268 – 2703
    Helixi273 – 2808
    Beta strandi283 – 2897
    Beta strandi292 – 2943
    Beta strandi299 – 3046
    Helixi307 – 3137
    Helixi316 – 3205
    Beta strandi326 – 3338
    Beta strandi336 – 34510
    Beta strandi350 – 3567
    Helixi357 – 3593
    Beta strandi361 – 3699
    Helixi373 – 38816

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2WZXX-ray1.40A27-397[»]
    2WZZX-ray1.57A27-397[»]
    3S1YX-ray1.40A27-397[»]
    3S22X-ray1.65A27-397[»]
    4GZBX-ray1.79A27-397[»]
    4HEFX-ray1.86A29-388[»]
    4NK3X-ray1.90A27-397[»]
    ProteinModelPortaliP24735.
    SMRiP24735. Positions 28-389.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP24735.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni342 – 3443Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-C beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1680.
    HOGENOMiHOG000267102.
    KOiK01467.
    OMAiKVGDMTQ.
    OrthoDBiEOG60SCH1.
    PhylomeDBiP24735.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR001586. Beta-lactam_class-C_AS.
    [Graphical view]
    PfamiPF00144. Beta-lactamase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24735-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRDTRFPCLC GIAASTLLFA TTPAIAGEAP ADRLKALVDA AVQPVMKAND    50
    IPGLAVAISL KGEPHYFSYG LASKEDGRRV TPETLFEIGS VSKTFTATLA 100
    GYALTQDKMR LDDRASQHWP ALQGSRFDGI SLLDLATYTA GGLPLQFPDS 150
    VQKDQAQIRD YYRQWQPTYA PGSQRLYSNP SIGLFGYLAA RSLGQPFERL 200
    MEQQVFPALG LEQTHLDVPE AALAQYAQGY GKDDRPLRVG PGPLDAEGYG 250
    VKTSAADLLR FVDANLHPER LDRPWAQALD ATHRGYYKVG DMTQGLGWEA 300
    YDWPISLKRL QAGNSTPMAL QPHRIARLPA PQALEGQRLL NKTGSTNGFG 350
    AYVAFVPGRD LGLVILANRN YPNAERVKIA YAILSGLEQQ GKVPLKR 397
    Length:397
    Mass (Da):43,401
    Last modified:December 8, 2000 - v2
    Checksum:iC6341A53594BC261
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti397 – 3971R → A in CAA38522. (PubMed:2125210)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54719 Genomic DNA. Translation: CAA38522.1.
    AE004091 Genomic DNA. Translation: AAG07497.1.
    X67095 Genomic DNA. Translation: CAA47469.1.
    PIRiF83132.
    S13408.
    RefSeqiNP_252799.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG07497; AAG07497; PA4110.
    GeneIDi878149.
    KEGGipae:PA4110.
    PATRICi19842900. VBIPseAer58763_4306.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54719 Genomic DNA. Translation: CAA38522.1 .
    AE004091 Genomic DNA. Translation: AAG07497.1 .
    X67095 Genomic DNA. Translation: CAA47469.1 .
    PIRi F83132.
    S13408.
    RefSeqi NP_252799.1. NC_002516.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2WZX X-ray 1.40 A 27-397 [» ]
    2WZZ X-ray 1.57 A 27-397 [» ]
    3S1Y X-ray 1.40 A 27-397 [» ]
    3S22 X-ray 1.65 A 27-397 [» ]
    4GZB X-ray 1.79 A 27-397 [» ]
    4HEF X-ray 1.86 A 29-388 [» ]
    4NK3 X-ray 1.90 A 27-397 [» ]
    ProteinModelPortali P24735.
    SMRi P24735. Positions 28-389.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA4110.

    Chemistry

    BindingDBi P24735.
    ChEMBLi CHEMBL5031.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG07497 ; AAG07497 ; PA4110 .
    GeneIDi 878149.
    KEGGi pae:PA4110.
    PATRICi 19842900. VBIPseAer58763_4306.

    Organism-specific databases

    PseudoCAPi PA4110.

    Phylogenomic databases

    eggNOGi COG1680.
    HOGENOMi HOG000267102.
    KOi K01467.
    OMAi KVGDMTQ.
    OrthoDBi EOG60SCH1.
    PhylomeDBi P24735.

    Enzyme and pathway databases

    SABIO-RK P24735.

    Miscellaneous databases

    EvolutionaryTracei P24735.
    PROi P24735.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR001586. Beta-lactam_class-C_AS.
    [Graphical view ]
    Pfami PF00144. Beta-lactamase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00336. BETA_LACTAMASE_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase."
      Lodge J.M., Minchin S.D., Piddock L.J.V., Busby S.J.W.
      Biochem. J. 272:627-631(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    3. "Two-dimensional polyacrylamide gel electrophoresis isolation and microsequencing of Pseudomonas aeruginosa proteins."
      Michea-Hamzehpour M., Sanchez J.-C., Epp S.F., Paquet N., Hughes G.J., Hochstrasser D.F., Pechere J.-C.
      Enzyme Protein 47:1-8(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 27-41.
    4. "Investigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampC beta-lactamase promoter."
      Lodge J.M., Busby S.J.W., Piddock L.J.V.
      FEMS Microbiol. Lett. 111:315-320(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3.
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiAMPC_PSEAE
    AccessioniPrimary (citable) accession number: P24735
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: December 8, 2000
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3