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P24735

- AMPC_PSEAE

UniProt

P24735 - AMPC_PSEAE

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Protein

Beta-lactamase

Gene

ampC

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei177 – 1771Proton acceptorBy similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. antibiotic catabolic process Source: InterPro
  2. regulation of beta-lactamase activity Source: PseudoCAP
  3. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

SABIO-RKP24735.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Cephalosporinase
Gene namesi
Name:ampC
Ordered Locus Names:PA4110
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA4110.

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 397371Beta-lactamasePRO_0000016963Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi208964.PA4110.

Structurei

Secondary structure

1
397
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 4819Combined sources
Beta strandi52 – 609Combined sources
Beta strandi63 – 7210Combined sources
Turni74 – 763Combined sources
Beta strandi85 – 873Combined sources
Helixi89 – 913Combined sources
Helixi92 – 10514Combined sources
Helixi115 – 1184Combined sources
Helixi120 – 1223Combined sources
Helixi126 – 1294Combined sources
Helixi132 – 1365Combined sources
Helixi155 – 16410Combined sources
Beta strandi173 – 1753Combined sources
Helixi179 – 19214Combined sources
Helixi197 – 2037Combined sources
Helixi205 – 2084Combined sources
Beta strandi212 – 2176Combined sources
Helixi220 – 2256Combined sources
Helixi232 – 2343Combined sources
Helixi245 – 2495Combined sources
Beta strandi252 – 2543Combined sources
Helixi255 – 26612Combined sources
Helixi268 – 2703Combined sources
Helixi273 – 2808Combined sources
Beta strandi283 – 2897Combined sources
Beta strandi292 – 2943Combined sources
Beta strandi299 – 3046Combined sources
Helixi307 – 3137Combined sources
Helixi316 – 3205Combined sources
Beta strandi326 – 3338Combined sources
Beta strandi336 – 34611Combined sources
Beta strandi349 – 3568Combined sources
Helixi357 – 3593Combined sources
Beta strandi361 – 3699Combined sources
Helixi373 – 38614Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WZXX-ray1.40A27-397[»]
2WZZX-ray1.57A27-397[»]
3S1YX-ray1.40A27-397[»]
3S22X-ray1.65A27-397[»]
4GZBX-ray1.79A27-397[»]
4HEFX-ray1.86A29-388[»]
4NK3X-ray1.90A27-397[»]
4OOYX-ray1.10A29-387[»]
ProteinModelPortaliP24735.
SMRiP24735. Positions 28-389.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24735.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni342 – 3443Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-C beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1680.
HOGENOMiHOG000267102.
InParanoidiP24735.
KOiK01467.
OMAiKVGDMTQ.
OrthoDBiEOG60SCH1.
PhylomeDBiP24735.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24735-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRDTRFPCLC GIAASTLLFA TTPAIAGEAP ADRLKALVDA AVQPVMKAND
60 70 80 90 100
IPGLAVAISL KGEPHYFSYG LASKEDGRRV TPETLFEIGS VSKTFTATLA
110 120 130 140 150
GYALTQDKMR LDDRASQHWP ALQGSRFDGI SLLDLATYTA GGLPLQFPDS
160 170 180 190 200
VQKDQAQIRD YYRQWQPTYA PGSQRLYSNP SIGLFGYLAA RSLGQPFERL
210 220 230 240 250
MEQQVFPALG LEQTHLDVPE AALAQYAQGY GKDDRPLRVG PGPLDAEGYG
260 270 280 290 300
VKTSAADLLR FVDANLHPER LDRPWAQALD ATHRGYYKVG DMTQGLGWEA
310 320 330 340 350
YDWPISLKRL QAGNSTPMAL QPHRIARLPA PQALEGQRLL NKTGSTNGFG
360 370 380 390
AYVAFVPGRD LGLVILANRN YPNAERVKIA YAILSGLEQQ GKVPLKR
Length:397
Mass (Da):43,401
Last modified:December 8, 2000 - v2
Checksum:iC6341A53594BC261
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti397 – 3971R → A in CAA38522. (PubMed:2125210)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54719 Genomic DNA. Translation: CAA38522.1.
AE004091 Genomic DNA. Translation: AAG07497.1.
X67095 Genomic DNA. Translation: CAA47469.1.
PIRiF83132.
S13408.
RefSeqiNP_252799.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG07497; AAG07497; PA4110.
GeneIDi878149.
KEGGipae:PA4110.
PATRICi19842900. VBIPseAer58763_4306.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54719 Genomic DNA. Translation: CAA38522.1 .
AE004091 Genomic DNA. Translation: AAG07497.1 .
X67095 Genomic DNA. Translation: CAA47469.1 .
PIRi F83132.
S13408.
RefSeqi NP_252799.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2WZX X-ray 1.40 A 27-397 [» ]
2WZZ X-ray 1.57 A 27-397 [» ]
3S1Y X-ray 1.40 A 27-397 [» ]
3S22 X-ray 1.65 A 27-397 [» ]
4GZB X-ray 1.79 A 27-397 [» ]
4HEF X-ray 1.86 A 29-388 [» ]
4NK3 X-ray 1.90 A 27-397 [» ]
4OOY X-ray 1.10 A 29-387 [» ]
ProteinModelPortali P24735.
SMRi P24735. Positions 28-389.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA4110.

Chemistry

BindingDBi P24735.
ChEMBLi CHEMBL5031.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG07497 ; AAG07497 ; PA4110 .
GeneIDi 878149.
KEGGi pae:PA4110.
PATRICi 19842900. VBIPseAer58763_4306.

Organism-specific databases

PseudoCAPi PA4110.

Phylogenomic databases

eggNOGi COG1680.
HOGENOMi HOG000267102.
InParanoidi P24735.
KOi K01467.
OMAi KVGDMTQ.
OrthoDBi EOG60SCH1.
PhylomeDBi P24735.

Enzyme and pathway databases

SABIO-RK P24735.

Miscellaneous databases

EvolutionaryTracei P24735.
PROi P24735.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view ]
Pfami PF00144. Beta-lactamase. 1 hit.
[Graphical view ]
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase."
    Lodge J.M., Minchin S.D., Piddock L.J.V., Busby S.J.W.
    Biochem. J. 272:627-631(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  3. "Two-dimensional polyacrylamide gel electrophoresis isolation and microsequencing of Pseudomonas aeruginosa proteins."
    Michea-Hamzehpour M., Sanchez J.-C., Epp S.F., Paquet N., Hughes G.J., Hochstrasser D.F., Pechere J.-C.
    Enzyme Protein 47:1-8(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-41.
  4. "Investigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampC beta-lactamase promoter."
    Lodge J.M., Busby S.J.W., Piddock L.J.V.
    FEMS Microbiol. Lett. 111:315-320(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiAMPC_PSEAE
AccessioniPrimary (citable) accession number: P24735
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: December 8, 2000
Last modified: November 26, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3