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Protein

Beta-lactamase

Gene

ampC

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei90Acyl-ester intermediatePROSITE-ProRule annotation1
Active sitei177Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

SABIO-RKP24735.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Cephalosporinase
Gene namesi
Name:ampC
Ordered Locus Names:PA4110
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4110.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5031.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 261 PublicationAdd BLAST26
ChainiPRO_000001696327 – 397Beta-lactamaseAdd BLAST371

Proteomic databases

PaxDbiP24735.
PRIDEiP24735.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA4110.

Chemistry databases

BindingDBiP24735.

Structurei

Secondary structure

1397
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 48Combined sources19
Beta strandi52 – 60Combined sources9
Beta strandi63 – 73Combined sources11
Turni74 – 77Combined sources4
Beta strandi85 – 87Combined sources3
Helixi89 – 91Combined sources3
Helixi92 – 105Combined sources14
Helixi115 – 118Combined sources4
Helixi120 – 122Combined sources3
Helixi126 – 129Combined sources4
Helixi132 – 136Combined sources5
Helixi155 – 164Combined sources10
Beta strandi173 – 175Combined sources3
Helixi179 – 192Combined sources14
Helixi197 – 203Combined sources7
Helixi205 – 208Combined sources4
Beta strandi212 – 217Combined sources6
Helixi220 – 225Combined sources6
Helixi232 – 234Combined sources3
Helixi245 – 249Combined sources5
Beta strandi252 – 254Combined sources3
Helixi255 – 266Combined sources12
Helixi268 – 270Combined sources3
Helixi273 – 280Combined sources8
Beta strandi283 – 289Combined sources7
Beta strandi292 – 294Combined sources3
Beta strandi299 – 304Combined sources6
Helixi307 – 313Combined sources7
Helixi316 – 320Combined sources5
Beta strandi326 – 333Combined sources8
Beta strandi336 – 346Combined sources11
Beta strandi349 – 356Combined sources8
Helixi357 – 359Combined sources3
Beta strandi361 – 369Combined sources9
Helixi373 – 387Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WZXX-ray1.40A27-397[»]
2WZZX-ray1.57A27-397[»]
3S1YX-ray1.40A27-397[»]
3S22X-ray1.65A27-397[»]
4GZBX-ray1.79A27-397[»]
4HEFX-ray1.86A29-388[»]
4NK3X-ray1.90A27-397[»]
4OOYX-ray1.10A29-387[»]
4WYYX-ray1.28A29-388[»]
4WZ4X-ray1.05A29-388[»]
4X68X-ray1.68A/B32-387[»]
ProteinModelPortaliP24735.
SMRiP24735.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24735.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni342 – 344Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the class-C beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG41066YJ. Bacteria.
COG1680. LUCA.
HOGENOMiHOG000267102.
InParanoidiP24735.
KOiK20320.
OMAiVMLANRN.
PhylomeDBiP24735.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24735-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDTRFPCLC GIAASTLLFA TTPAIAGEAP ADRLKALVDA AVQPVMKAND
60 70 80 90 100
IPGLAVAISL KGEPHYFSYG LASKEDGRRV TPETLFEIGS VSKTFTATLA
110 120 130 140 150
GYALTQDKMR LDDRASQHWP ALQGSRFDGI SLLDLATYTA GGLPLQFPDS
160 170 180 190 200
VQKDQAQIRD YYRQWQPTYA PGSQRLYSNP SIGLFGYLAA RSLGQPFERL
210 220 230 240 250
MEQQVFPALG LEQTHLDVPE AALAQYAQGY GKDDRPLRVG PGPLDAEGYG
260 270 280 290 300
VKTSAADLLR FVDANLHPER LDRPWAQALD ATHRGYYKVG DMTQGLGWEA
310 320 330 340 350
YDWPISLKRL QAGNSTPMAL QPHRIARLPA PQALEGQRLL NKTGSTNGFG
360 370 380 390
AYVAFVPGRD LGLVILANRN YPNAERVKIA YAILSGLEQQ GKVPLKR
Length:397
Mass (Da):43,401
Last modified:December 8, 2000 - v2
Checksum:iC6341A53594BC261
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti397R → A in CAA38522 (PubMed:2125210).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54719 Genomic DNA. Translation: CAA38522.1.
AE004091 Genomic DNA. Translation: AAG07497.1.
X67095 Genomic DNA. Translation: CAA47469.1.
PIRiF83132.
S13408.
RefSeqiNP_252799.1. NC_002516.2.
WP_003101289.1. NZ_ASJY01000658.1.

Genome annotation databases

EnsemblBacteriaiAAG07497; AAG07497; PA4110.
GeneIDi878149.
KEGGipae:PA4110.
PATRICi19842900. VBIPseAer58763_4306.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54719 Genomic DNA. Translation: CAA38522.1.
AE004091 Genomic DNA. Translation: AAG07497.1.
X67095 Genomic DNA. Translation: CAA47469.1.
PIRiF83132.
S13408.
RefSeqiNP_252799.1. NC_002516.2.
WP_003101289.1. NZ_ASJY01000658.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WZXX-ray1.40A27-397[»]
2WZZX-ray1.57A27-397[»]
3S1YX-ray1.40A27-397[»]
3S22X-ray1.65A27-397[»]
4GZBX-ray1.79A27-397[»]
4HEFX-ray1.86A29-388[»]
4NK3X-ray1.90A27-397[»]
4OOYX-ray1.10A29-387[»]
4WYYX-ray1.28A29-388[»]
4WZ4X-ray1.05A29-388[»]
4X68X-ray1.68A/B32-387[»]
ProteinModelPortaliP24735.
SMRiP24735.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4110.

Chemistry databases

BindingDBiP24735.
ChEMBLiCHEMBL5031.

Proteomic databases

PaxDbiP24735.
PRIDEiP24735.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07497; AAG07497; PA4110.
GeneIDi878149.
KEGGipae:PA4110.
PATRICi19842900. VBIPseAer58763_4306.

Organism-specific databases

PseudoCAPiPA4110.

Phylogenomic databases

eggNOGiENOG41066YJ. Bacteria.
COG1680. LUCA.
HOGENOMiHOG000267102.
InParanoidiP24735.
KOiK20320.
OMAiVMLANRN.
PhylomeDBiP24735.

Enzyme and pathway databases

SABIO-RKP24735.

Miscellaneous databases

EvolutionaryTraceiP24735.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPC_PSEAE
AccessioniPrimary (citable) accession number: P24735
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: December 8, 2000
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.