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P24735

- AMPC_PSEAE

UniProt

P24735 - AMPC_PSEAE

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Protein

Beta-lactamase

Gene
ampC, PA4110
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901Acyl-ester intermediate By similarity
Active sitei177 – 1771Proton acceptor By similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. antibiotic catabolic process Source: InterPro
  2. regulation of beta-lactamase activity Source: PseudoCAP
  3. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

SABIO-RKP24735.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Cephalosporinase
Gene namesi
Name:ampC
Ordered Locus Names:PA4110
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA4110.

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 397371Beta-lactamasePRO_0000016963Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi208964.PA4110.

Structurei

Secondary structure

1
397
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 4820
Beta strandi52 – 609
Beta strandi63 – 7210
Turni74 – 763
Beta strandi85 – 873
Helixi89 – 913
Helixi92 – 10514
Helixi115 – 1184
Helixi120 – 1223
Helixi126 – 1294
Helixi132 – 1365
Helixi155 – 16410
Beta strandi173 – 1753
Helixi179 – 19214
Helixi197 – 2037
Helixi205 – 2084
Beta strandi212 – 2176
Helixi220 – 2256
Helixi232 – 2343
Helixi245 – 2495
Beta strandi252 – 2543
Helixi255 – 26612
Helixi268 – 2703
Helixi273 – 2808
Beta strandi283 – 2897
Beta strandi292 – 2943
Beta strandi299 – 3046
Helixi307 – 3137
Helixi316 – 3205
Beta strandi326 – 3338
Beta strandi336 – 34510
Beta strandi350 – 3567
Helixi357 – 3593
Beta strandi361 – 3699
Helixi373 – 38816

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WZXX-ray1.40A27-397[»]
2WZZX-ray1.57A27-397[»]
3S1YX-ray1.40A27-397[»]
3S22X-ray1.65A27-397[»]
4GZBX-ray1.79A27-397[»]
4HEFX-ray1.86A29-388[»]
4NK3X-ray1.90A27-397[»]
ProteinModelPortaliP24735.
SMRiP24735. Positions 28-389.

Miscellaneous databases

EvolutionaryTraceiP24735.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni342 – 3443Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1680.
HOGENOMiHOG000267102.
KOiK01467.
OMAiKVGDMTQ.
OrthoDBiEOG60SCH1.
PhylomeDBiP24735.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24735-1 [UniParc]FASTAAdd to Basket

« Hide

MRDTRFPCLC GIAASTLLFA TTPAIAGEAP ADRLKALVDA AVQPVMKAND    50
IPGLAVAISL KGEPHYFSYG LASKEDGRRV TPETLFEIGS VSKTFTATLA 100
GYALTQDKMR LDDRASQHWP ALQGSRFDGI SLLDLATYTA GGLPLQFPDS 150
VQKDQAQIRD YYRQWQPTYA PGSQRLYSNP SIGLFGYLAA RSLGQPFERL 200
MEQQVFPALG LEQTHLDVPE AALAQYAQGY GKDDRPLRVG PGPLDAEGYG 250
VKTSAADLLR FVDANLHPER LDRPWAQALD ATHRGYYKVG DMTQGLGWEA 300
YDWPISLKRL QAGNSTPMAL QPHRIARLPA PQALEGQRLL NKTGSTNGFG 350
AYVAFVPGRD LGLVILANRN YPNAERVKIA YAILSGLEQQ GKVPLKR 397
Length:397
Mass (Da):43,401
Last modified:December 8, 2000 - v2
Checksum:iC6341A53594BC261
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti397 – 3971R → A in CAA38522. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54719 Genomic DNA. Translation: CAA38522.1.
AE004091 Genomic DNA. Translation: AAG07497.1.
X67095 Genomic DNA. Translation: CAA47469.1.
PIRiF83132.
S13408.
RefSeqiNP_252799.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG07497; AAG07497; PA4110.
GeneIDi878149.
KEGGipae:PA4110.
PATRICi19842900. VBIPseAer58763_4306.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54719 Genomic DNA. Translation: CAA38522.1 .
AE004091 Genomic DNA. Translation: AAG07497.1 .
X67095 Genomic DNA. Translation: CAA47469.1 .
PIRi F83132.
S13408.
RefSeqi NP_252799.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2WZX X-ray 1.40 A 27-397 [» ]
2WZZ X-ray 1.57 A 27-397 [» ]
3S1Y X-ray 1.40 A 27-397 [» ]
3S22 X-ray 1.65 A 27-397 [» ]
4GZB X-ray 1.79 A 27-397 [» ]
4HEF X-ray 1.86 A 29-388 [» ]
4NK3 X-ray 1.90 A 27-397 [» ]
ProteinModelPortali P24735.
SMRi P24735. Positions 28-389.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA4110.

Chemistry

BindingDBi P24735.
ChEMBLi CHEMBL5031.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG07497 ; AAG07497 ; PA4110 .
GeneIDi 878149.
KEGGi pae:PA4110.
PATRICi 19842900. VBIPseAer58763_4306.

Organism-specific databases

PseudoCAPi PA4110.

Phylogenomic databases

eggNOGi COG1680.
HOGENOMi HOG000267102.
KOi K01467.
OMAi KVGDMTQ.
OrthoDBi EOG60SCH1.
PhylomeDBi P24735.

Enzyme and pathway databases

SABIO-RK P24735.

Miscellaneous databases

EvolutionaryTracei P24735.
PROi P24735.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view ]
Pfami PF00144. Beta-lactamase. 1 hit.
[Graphical view ]
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing and analysis of the structural gene and regulatory region of the Pseudomonas aeruginosa chromosomal ampC beta-lactamase."
    Lodge J.M., Minchin S.D., Piddock L.J.V., Busby S.J.W.
    Biochem. J. 272:627-631(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  3. "Two-dimensional polyacrylamide gel electrophoresis isolation and microsequencing of Pseudomonas aeruginosa proteins."
    Michea-Hamzehpour M., Sanchez J.-C., Epp S.F., Paquet N., Hughes G.J., Hochstrasser D.F., Pechere J.-C.
    Enzyme Protein 47:1-8(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-41.
  4. "Investigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampC beta-lactamase promoter."
    Lodge J.M., Busby S.J.W., Piddock L.J.V.
    FEMS Microbiol. Lett. 111:315-320(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiAMPC_PSEAE
AccessioniPrimary (citable) accession number: P24735
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: December 8, 2000
Last modified: July 9, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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