Reviewed, UniProtKB/Swiss-Prot P24722 (KCRT_ONCMY)

Names and origin

Protein names

Recommended name:
    Creatine kinase, testis isozyme
    EC=2.7.3.2

Gene names

Name:

tck1

Organism

Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)

Taxonomic identifier

8022 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Protacanthopterygii › Salmoniformes › Salmonidae › Salmoninae › Oncorhynchus

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Catalytic activity	ATP + creatine = ADP + phosphocreatine.
Tissue specificity	Exists in many tissues, but preferentially in testis. Ref.1
Sequence similarities	Belongs to the ATP:guanido phosphotransferase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW creatine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	Creatine kinase, testis isozyme		PRO_0000211984
Regions
	Nucleotide binding	130 – 134	5	ATP By similarity
	Nucleotide binding	322 – 327	6	ATP By similarity
Sites
	Binding site	193	1	ATP By similarity
	Binding site	238	1	ATP By similarity
	Binding site	294	1	ATP By similarity
	Binding site	337	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24722-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 608BAAA115C7D1EA	FASTA	383	43,004
	10 20 30 40 50 60 MEKINDKMAK LTIKRLSPEE EFPDLSQHNN HMAKVLTQDM YTKLRDRATP NGFTIDGVIQ 70 80 90 100 110 120 TGIDNPGHPF IMTVGCVAGD EETYEVFKEL LDPIIEDRHG GYKPTDKHKT DLNPDNLKGG 130 140 150 160 170 180 DDLDPNYVIS SRVRTGRSIR GFCLPPHCSR GERRGVEKMS VEALDSLSGD LKGKYYALKN 190 200 210 220 230 240 MTDAEQQQLI DDHFLFDKPV SPLLLASGMA RDWPDGRGIW HNDTKTFLVW VNEEDHLRVI 250 260 270 280 290 300 SMQKGGNMKE VFNRFCTGLT KIETLFKDKG TSFMWNEHLG YVLTCPSNLG TGLRAGVHVK 310 320 330 340 350 360 IPNISKHAKF EEVLKRLRLQ KRGTGGVDTA AVGGTFDISN ADRLGFSEVE LVQMVVDGVK 370 380 LLVEMEKKLE KGQSIDDLMP AQK

References

[1]	"A novel creatine kinase cDNA whose transcript shows enhanced testicular expression." Garber A.T., Winkfein R.J., Dixon G.H. Biochim. Biophys. Acta 1087:256-258(1990) [PubMed: 2223887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X53859 mRNA. Translation: CAA37852.1.
PIR	S13164.
RefSeq	NP_001118187.1.
UniGene	Omy.35804
3D structure databases
HSSP	HSSP built from PDB template 2CRK based on UniProtKB P00563.
SMR	P24722. Positions 15-383.
ModBase	Search...
Genome annotation databases
GeneID	100136767.
Phylogenomic databases
HOVERGEN	P24722.
Enzyme and pathway databases
BRENDA	2.7.3.2. 3435.
Family and domain databases
InterPro	IPR000749. ATP-guanido_PTrfase. IPR014746. Gln_synth/guanido_kin_cat. [Graphical view]
Gene3D	G3DSA:1.10.135.10. ATP-gua_Ptrans. 1 hit. G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
PANTHER	PTHR11547. ATP-gua_Ptrans. 1 hit.
Pfam	PF00217. ATP-gua_Ptrans. 1 hit. PF02807. ATP-gua_PtransN. 1 hit. [Graphical view]
PROSITE	PS00112. GUANIDO_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KCRT_ONCMY

Accession

Primary (citable) accession number: P24722

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	March 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)