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P24722

- KCRT_ONCMY

UniProt

P24722 - KCRT_ONCMY

Protein

Creatine kinase, testis isozyme

Gene

tck1

Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

    Catalytic activityi

    ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei193 – 1931ATPPROSITE-ProRule annotation
    Binding sitei238 – 2381ATPPROSITE-ProRule annotation
    Binding sitei294 – 2941ATPPROSITE-ProRule annotation
    Binding sitei337 – 3371ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi130 – 1345ATPPROSITE-ProRule annotation
    Nucleotide bindingi322 – 3276ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. creatine kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Creatine kinase, testis isozyme (EC:2.7.3.2)
    Gene namesi
    Name:tck1
    OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
    Taxonomic identifieri8022 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 383383Creatine kinase, testis isozymePRO_0000211984Add
    BLAST

    Proteomic databases

    PRIDEiP24722.

    Expressioni

    Tissue specificityi

    Exists in many tissues, but preferentially in testis.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP24722.
    SMRiP24722. Positions 15-383.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 10087Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini127 – 369243Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG001339.

    Family and domain databases

    Gene3Di1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view]
    PfamiPF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view]
    SUPFAMiSSF48034. SSF48034. 1 hit.
    PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24722-1 [UniParc]FASTAAdd to Basket

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    MEKINDKMAK LTIKRLSPEE EFPDLSQHNN HMAKVLTQDM YTKLRDRATP    50
    NGFTIDGVIQ TGIDNPGHPF IMTVGCVAGD EETYEVFKEL LDPIIEDRHG 100
    GYKPTDKHKT DLNPDNLKGG DDLDPNYVIS SRVRTGRSIR GFCLPPHCSR 150
    GERRGVEKMS VEALDSLSGD LKGKYYALKN MTDAEQQQLI DDHFLFDKPV 200
    SPLLLASGMA RDWPDGRGIW HNDTKTFLVW VNEEDHLRVI SMQKGGNMKE 250
    VFNRFCTGLT KIETLFKDKG TSFMWNEHLG YVLTCPSNLG TGLRAGVHVK 300
    IPNISKHAKF EEVLKRLRLQ KRGTGGVDTA AVGGTFDISN ADRLGFSEVE 350
    LVQMVVDGVK LLVEMEKKLE KGQSIDDLMP AQK 383
    Length:383
    Mass (Da):43,004
    Last modified:March 1, 1992 - v1
    Checksum:i608BAAA115C7D1EA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53859 mRNA. Translation: CAA37852.1.
    PIRiS13164.
    RefSeqiNP_001118187.1. NM_001124715.1.
    UniGeneiOmy.35804.

    Genome annotation databases

    GeneIDi100136767.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53859 mRNA. Translation: CAA37852.1 .
    PIRi S13164.
    RefSeqi NP_001118187.1. NM_001124715.1.
    UniGenei Omy.35804.

    3D structure databases

    ProteinModelPortali P24722.
    SMRi P24722. Positions 15-383.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P24722.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100136767.

    Organism-specific databases

    CTDi 1152.

    Phylogenomic databases

    HOVERGENi HBG001339.

    Family and domain databases

    Gene3Di 1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view ]
    Pfami PF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48034. SSF48034. 1 hit.
    PROSITEi PS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel creatine kinase cDNA whose transcript shows enhanced testicular expression."
      Garber A.T., Winkfein R.J., Dixon G.H.
      Biochim. Biophys. Acta 1087:256-258(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Testis.

    Entry informationi

    Entry nameiKCRT_ONCMY
    AccessioniPrimary (citable) accession number: P24722
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3