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Protein

Creatine kinase, testis isozyme

Gene

tck1

Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei193ATPPROSITE-ProRule annotation1
Binding sitei238ATPPROSITE-ProRule annotation1
Binding sitei294ATPPROSITE-ProRule annotation1
Binding sitei337ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi130 – 134ATPPROSITE-ProRule annotation5
Nucleotide bindingi322 – 327ATPPROSITE-ProRule annotation6

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase, testis isozyme (EC:2.7.3.2)
Gene namesi
Name:tck1
OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifieri8022 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002119841 – 383Creatine kinase, testis isozymeAdd BLAST383

Proteomic databases

PRIDEiP24722.

Expressioni

Tissue specificityi

Exists in many tissues, but preferentially in testis.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP24722.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 100Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST87
Domaini127 – 369Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG001339.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKINDKMAK LTIKRLSPEE EFPDLSQHNN HMAKVLTQDM YTKLRDRATP
60 70 80 90 100
NGFTIDGVIQ TGIDNPGHPF IMTVGCVAGD EETYEVFKEL LDPIIEDRHG
110 120 130 140 150
GYKPTDKHKT DLNPDNLKGG DDLDPNYVIS SRVRTGRSIR GFCLPPHCSR
160 170 180 190 200
GERRGVEKMS VEALDSLSGD LKGKYYALKN MTDAEQQQLI DDHFLFDKPV
210 220 230 240 250
SPLLLASGMA RDWPDGRGIW HNDTKTFLVW VNEEDHLRVI SMQKGGNMKE
260 270 280 290 300
VFNRFCTGLT KIETLFKDKG TSFMWNEHLG YVLTCPSNLG TGLRAGVHVK
310 320 330 340 350
IPNISKHAKF EEVLKRLRLQ KRGTGGVDTA AVGGTFDISN ADRLGFSEVE
360 370 380
LVQMVVDGVK LLVEMEKKLE KGQSIDDLMP AQK
Length:383
Mass (Da):43,004
Last modified:March 1, 1992 - v1
Checksum:i608BAAA115C7D1EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53859 mRNA. Translation: CAA37852.1.
PIRiS13164.
RefSeqiNP_001118187.1. NM_001124715.1.
UniGeneiOmy.35804.

Genome annotation databases

GeneIDi100136767.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53859 mRNA. Translation: CAA37852.1.
PIRiS13164.
RefSeqiNP_001118187.1. NM_001124715.1.
UniGeneiOmy.35804.

3D structure databases

ProteinModelPortaliP24722.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP24722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100136767.

Organism-specific databases

CTDi1152.

Phylogenomic databases

HOVERGENiHBG001339.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCRT_ONCMY
AccessioniPrimary (citable) accession number: P24722
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: October 5, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.