P24717 (RNAS1_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease pancreatic
EC=3.1.27.5

Alternative name(s):
RNase 1
RNase A

Gene names

Name:	RNASE1
Synonyms:	RNS1

Organism

Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	130 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – 6	›6
	Chain	7 – 130	124	Ribonuclease pancreatic		PRO_0000030918
Regions
	Region	47 – 51	5	Substrate binding By similarity
Sites
	Active site	18	1	Proton acceptor By similarity
	Active site	125	1	Proton donor By similarity
	Binding site	13	1	Substrate By similarity
	Binding site	16	1	Substrate By similarity
	Binding site	72	1	Substrate By similarity
	Binding site	91	1	Substrate By similarity
Amino acid modifications
	Glycosylation	40	1	N-linked (GlcNAc...) Probable
	Disulfide bond	32 ↔ 90		By similarity
	Disulfide bond	46 ↔ 101		By similarity
	Disulfide bond	64 ↔ 116		By similarity
	Disulfide bond	71 ↔ 78		By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P24717 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 8D227FD6491B413D	FASTA	130	14,517
	10 20 30 40 50 60 VQPSLGKESA AMKFERQHMD STVATSSSPT YCNQMMKRRN MTQGQCKPVN TFVHESLADV 70 80 90 100 110 120 HAVCSQENVK CKNGKSNCYK SHSALHITDC RLKGNAKYPN CDYQTSQHQK HIIVACEGNP 130 FVPVHFDATV

References

[1]	"The DNA sequences of the human and hamster secretory ribonucleases determined with the polymerase chain reaction (PCR)." Haugg M., Schein C.H. Nucleic Acids Res. 20:612-612(1992) [PubMed: 1741299] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Ovary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62945 Genomic DNA. Translation: CAA44717.1.
PIR	S22808.
3D structure databases
ProteinModelPortal	P24717.
SMR	P24717. Positions 7-130.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008396.
Family and domain databases
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_CRIGR

Accession

Primary (citable) accession number: P24717

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program