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Protein

Myogenic factor 5

Gene

Myf5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.1 Publication

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: GO_Central
  2. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
  3. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
  4. sequence-specific DNA binding Source: MGI

GO - Biological processi

  1. camera-type eye development Source: MGI
  2. cartilage condensation Source: MGI
  3. embryonic skeletal system morphogenesis Source: MGI
  4. extracellular matrix organization Source: MGI
  5. muscle organ development Source: MGI
  6. muscle organ morphogenesis Source: MGI
  7. muscle tissue morphogenesis Source: MGI
  8. ossification Source: MGI
  9. positive regulation of myoblast differentiation Source: GO_Central
  10. positive regulation of myoblast fusion Source: GO_Central
  11. positive regulation of skeletal muscle fiber development Source: GO_Central
  12. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  13. regulation of cell-matrix adhesion Source: MGI
  14. skeletal muscle cell differentiation Source: MGI
  15. skeletal muscle tissue development Source: MGI
  16. somitogenesis Source: MGI
  17. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Myogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myogenic factor 5
Short name:
Myf-5
Gene namesi
Name:Myf5
Synonyms:Myf-5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:97252. Myf5.

Subcellular locationi

GO - Cellular componenti

  1. nucleoplasm Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 255255Myogenic factor 5PRO_0000127345Add
BLAST

Proteomic databases

PRIDEiP24699.

PTM databases

PhosphoSiteiP24699.

Expressioni

Gene expression databases

BgeeiP24699.
CleanExiMM_MYF5.
ExpressionAtlasiP24699. baseline and differential.
GenevestigatoriP24699.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein.

Protein-protein interaction databases

BioGridi201641. 1 interaction.
MINTiMINT-1519267.
STRINGi10090.ENSMUSP00000000445.

Structurei

3D structure databases

ProteinModelPortaliP24699.
SMRiP24699. Positions 76-140.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini83 – 13452bHLHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG239177.
GeneTreeiENSGT00530000063004.
HOGENOMiHOG000234800.
HOVERGENiHBG006429.
InParanoidiP24699.
KOiK18484.
OMAiDMMDGCQ.
OrthoDBiEOG76QFK1.
PhylomeDBiP24699.
TreeFamiTF316344.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR002546. Basic.
IPR011598. bHLH_dom.
IPR022032. Myf5.
[Graphical view]
PfamiPF01586. Basic. 1 hit.
PF00010. HLH. 1 hit.
PF12232. Myf5. 1 hit.
[Graphical view]
SMARTiSM00520. BASIC. 1 hit.
SM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24699-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDMTDGCQFS PSEYFYEGSC IPSPEDEFGD QFEPRVAAFG AHKAELQGSD
60 70 80 90 100
DEEHVRAPTG HHQAGHCLMW ACKACKRKST TMDRRKAATM RERRRLKKVN
110 120 130 140 150
QAFETLKRCT TTNPNQRLPK VEILRNAIRY IESLQELLRE QVENYYSLPG
160 170 180 190 200
QSCSEPTSPT SNCSDGMPEC NSPVWSRKNS SFDSIYCPDV SNACAADKSS
210 220 230 240 250
VSSLDCLSSI VDRITSTEPS ELALQDTASL SPATSANSQP ATPGPSSSRL

IYHVL
Length:255
Mass (Da):28,229
Last modified:February 29, 1992 - v1
Checksum:i0BDCBEDF2ABCAEC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56182 mRNA. Translation: CAA39643.1.
AK044894 mRNA. Translation: BAC32132.1.
AK083402 mRNA. Translation: BAC38902.1.
CCDSiCCDS24161.1.
PIRiS22825.
RefSeqiNP_032682.1. NM_008656.5.
XP_006513382.1. XM_006513319.2.
UniGeneiMm.4984.

Genome annotation databases

EnsembliENSMUST00000000445; ENSMUSP00000000445; ENSMUSG00000000435.
GeneIDi17877.
KEGGimmu:17877.
UCSCiuc007gyz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56182 mRNA. Translation: CAA39643.1.
AK044894 mRNA. Translation: BAC32132.1.
AK083402 mRNA. Translation: BAC38902.1.
CCDSiCCDS24161.1.
PIRiS22825.
RefSeqiNP_032682.1. NM_008656.5.
XP_006513382.1. XM_006513319.2.
UniGeneiMm.4984.

3D structure databases

ProteinModelPortaliP24699.
SMRiP24699. Positions 76-140.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201641. 1 interaction.
MINTiMINT-1519267.
STRINGi10090.ENSMUSP00000000445.

PTM databases

PhosphoSiteiP24699.

Proteomic databases

PRIDEiP24699.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000445; ENSMUSP00000000445; ENSMUSG00000000435.
GeneIDi17877.
KEGGimmu:17877.
UCSCiuc007gyz.1. mouse.

Organism-specific databases

CTDi4617.
MGIiMGI:97252. Myf5.

Phylogenomic databases

eggNOGiNOG239177.
GeneTreeiENSGT00530000063004.
HOGENOMiHOG000234800.
HOVERGENiHBG006429.
InParanoidiP24699.
KOiK18484.
OMAiDMMDGCQ.
OrthoDBiEOG76QFK1.
PhylomeDBiP24699.
TreeFamiTF316344.

Miscellaneous databases

NextBioi292665.
PROiP24699.
SOURCEiSearch...

Gene expression databases

BgeeiP24699.
CleanExiMM_MYF5.
ExpressionAtlasiP24699. baseline and differential.
GenevestigatoriP24699.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR002546. Basic.
IPR011598. bHLH_dom.
IPR022032. Myf5.
[Graphical view]
PfamiPF01586. Basic. 1 hit.
PF00010. HLH. 1 hit.
PF12232. Myf5. 1 hit.
[Graphical view]
SMARTiSM00520. BASIC. 1 hit.
SM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The MyoD family of myogenic factors is regulated by electrical activity: isolation and characterization of a mouse Myf-5 cDNA."
    Buonanno A., Apone L., Morasso M.I., Beers R., Brenner H.R., Eftimie R.
    Nucleic Acids Res. 20:539-544(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H.
    Tissue: Skeletal muscle and Smooth muscle.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  3. "Sequential association of myogenic regulatory factors and E proteins at muscle-specific genes."
    Londhe P., Davie J.K.
    Skelet. Muscle 1:14-14(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PROMOTER BINDING.

Entry informationi

Entry nameiMYF5_MOUSE
AccessioniPrimary (citable) accession number: P24699
Secondary accession number(s): Q543W7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 29, 1992
Last sequence update: February 29, 1992
Last modified: March 31, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.