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Protein

RNA polymerase sigma-54 factor

Gene

rpoN

Organism
Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core RNA polymerase) serves as the receptor for receipt of the melting signal from the remotely bound activator protein NifA for the expression of the nitrogen fixation proteins.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi365 – 384H-T-H motifSequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Sigma factor, Transferase
Biological processNitrogen fixation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma-54 factor
Gene namesi
Name:rpoN
Synonyms:ntrA
OrganismiAcidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)
Taxonomic identifieri920 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAcidithiobacilliaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002055441 – 475RNA polymerase sigma-54 factorAdd BLAST475

Structurei

3D structure databases

ProteinModelPortaliP24695.
SMRiP24695.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi453 – 461RPON box9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 38Gln-richAdd BLAST36

Sequence similaritiesi

Belongs to the sigma-54 factor family.Curated

Phylogenomic databases

eggNOGiENOG4105DTW. Bacteria.
COG1508. LUCA.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiView protein in InterPro
IPR000394. RNA_pol_sigma_54.
IPR007046. RNA_pol_sigma_54_core-bd.
IPR007634. RNA_pol_sigma_54_DNA-bd.
IPR036388. WH-like_DNA-bd_sf.
PANTHERiPTHR32248. PTHR32248. 1 hit.
PfamiView protein in Pfam
PF00309. Sigma54_AID. 1 hit.
PF04963. Sigma54_CBD. 1 hit.
PF04552. Sigma54_DBD. 1 hit.
PIRSFiPIRSF000774. RpoN. 1 hit.
PRINTSiPR00045. SIGMA54FCT.
TIGRFAMsiTIGR02395. rpoN_sigma. 1 hit.
PROSITEiView protein in PROSITE
PS00717. SIGMA54_1. 1 hit.
PS00718. SIGMA54_2. 1 hit.
PS50044. SIGMA54_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P24695-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQGLELKLG QHLAMTPQLQ QAIRLLQLST VDLQQEVQGM LESNPLLDEE
60 70 80 90 100
TGDEGGGGPI PETVELPSEE RQLDLAAENI LPDELPVDSQ WDDIFDMGTS
110 120 130 140 150
GSGNGSDEDL PDFESRNSRT QSLQDYLRWQ ADMTHFTADE RNMAELIIDA
160 170 180 190 200
IDERGYLADS LEDLAATMNV QEDALLAVLL RVQDFDPPGV GARNLSECLL
210 220 230 240 250
LQLKQMVEKD DAHVLLAQRI VKDHLQALGR HDYPRLCTVL GVDEAALRAA
260 270 280 290 300
MALISALNPK PGEDVGTEST EYVIPDVIVR WAGSRLRTDL NPEAMPKLRI
310 320 330 340 350
NRHYADMAGG KDAAHKYIQD QLNEARWFIK SLQSRQDTIL KVARAIVERQ
360 370 380 390 400
KDFFANGPES MRPMVLRHIA DAVEMHESTV SRVTNQKYMI TPRGLYEFKY
410 420 430 440 450
FFSSHVGTDS GGSASATAIR ALLIKMTQAE DAQHPLSDAE IARVLADQGI
460 470
QIARRTVAKY REAANVPPAS QRRRL
Length:475
Mass (Da):52,928
Last modified:March 1, 1992 - v1
Checksum:iB7B000DB08696305
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58480 Genomic DNA. Translation: AAA27379.1.
PIRiB37761.
RefSeqiWP_012537552.1. NZ_LQRJ01000049.1.

Similar proteinsi

Entry informationi

Entry nameiRP54_ACIFR
AccessioniPrimary (citable) accession number: P24695
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: October 25, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families