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Protein

Cation-dependent mannose-6-phosphate receptor

Gene

M6pr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.

GO - Molecular functioni

GO - Biological processi

  • intracellular protein transport Source: MGI
  • secretion of lysosomal enzymes Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
Cation-dependent mannose-6-phosphate receptor
Short name:
CD Man-6-P receptor
Short name:
CD-MPR
Alternative name(s):
46 kDa mannose 6-phosphate receptor
Short name:
MPR 46
Gene namesi
Name:M6pr
Synonyms:46mpr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:96904. M6pr.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 186165LumenalSequence analysisAdd
BLAST
Transmembranei187 – 21125HelicalSequence analysisAdd
BLAST
Topological domaini212 – 27867CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Add
BLAST
Chaini22 – 278257Cation-dependent mannose-6-phosphate receptorPRO_0000019227Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi58 – 581N-linked (GlcNAc...)Sequence analysis
Glycosylationi84 – 841N-linked (GlcNAc...)2 Publications
Glycosylationi95 – 951N-linked (GlcNAc...)Sequence analysis
Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence analysis
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence analysis
Modified residuei268 – 2681PhosphoserineCombined sources

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP24668.
PaxDbiP24668.
PRIDEiP24668.

PTM databases

iPTMnetiP24668.
PhosphoSiteiP24668.
SwissPalmiP24668.

Expressioni

Gene expression databases

BgeeiP24668.
CleanExiMM_M6PR.
ExpressionAtlasiP24668. baseline and differential.
GenevisibleiP24668. MM.

Interactioni

Subunit structurei

Homodimer. Binds GGA1, GGA2 and GGA3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi201263. 7 interactions.
IntActiP24668. 9 interactions.
MINTiMINT-4102408.
STRINGi10090.ENSMUSP00000007602.

Structurei

3D structure databases

ProteinModelPortaliP24668.
SMRiP24668. Positions 29-181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The extracellular domain is homologous to the repeating units (of approximately 147 AA) of the cation-independent mannose 6-phosphate receptor.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IK0D. Eukaryota.
ENOG4111GED. LUCA.
HOGENOMiHOG000013085.
HOVERGENiHBG006395.
InParanoidiP24668.
KOiK10089.
OMAiSDWIMLI.
OrthoDBiEOG7PP576.
PhylomeDBiP24668.
TreeFamiTF328910.

Family and domain databases

Gene3Di2.70.130.10. 1 hit.
InterProiIPR028927. Man-6-P_rcpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR000296. Man_6_P_rcpt.
[Graphical view]
PfamiPF02157. Man-6-P_recep. 1 hit.
[Graphical view]
PRINTSiPR00715. MAN6PRECEPTR.
SUPFAMiSSF50911. SSF50911. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24668-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFPFSGCWRT ELLLLLLLAV AVRESWQIEE KSCDLVGEKD KESKNEVALL
60 70 80 90 100
ERLRPLFNKS FESTVGQGSD TYSYIFRVCR EASNHSSGAG LVQINKSNDK
110 120 130 140 150
ETVVGRINET HIFNGSNWIM LIYKGGDEYD NHCGKEQRRA VVMISCNRHT
160 170 180 190 200
LAANFNPVSE ERGKVQDCFY LFEMDSSLAC SPEVSHLSVG SILLVIFASL
210 220 230 240 250
VAVYIIGGFL YQRLVVGAKG MEQFPHLAFW QDLGNLVADG CDFVCRSKPR
260 270
NVPAAYRGVG DDQLGEESEE RDDHLLPM
Length:278
Mass (Da):31,172
Last modified:March 1, 1992 - v1
Checksum:i8E0E8727CE793E7D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti196 – 1961I → T in AAA39482 (PubMed:1848553).Curated
Sequence conflicti221 – 2211M → T in AAA39482 (PubMed:1848553).Curated
Sequence conflicti226 – 2261H → P in AAA39482 (PubMed:1848553).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64068 mRNA. Translation: CAA45423.1.
X64070 mRNA. Translation: CAA45426.1.
X56831 mRNA. Translation: CAA40162.1.
M63286 mRNA. Translation: AAA39735.1.
BC027210 mRNA. Translation: AAH27210.1.
BC046956 mRNA. Translation: AAH46956.1.
BC080811 mRNA. Translation: AAH80811.1.
M58585 mRNA. Translation: AAA39482.1.
CCDSiCCDS20493.1.
PIRiA40399.
RefSeqiNP_034879.2. NM_010749.6.
UniGeneiMm.249225.

Genome annotation databases

EnsembliENSMUST00000007602; ENSMUSP00000007602; ENSMUSG00000007458.
ENSMUST00000112610; ENSMUSP00000108229; ENSMUSG00000007458.
GeneIDi17113.
KEGGimmu:17113.
UCSCiuc009dow.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64068 mRNA. Translation: CAA45423.1.
X64070 mRNA. Translation: CAA45426.1.
X56831 mRNA. Translation: CAA40162.1.
M63286 mRNA. Translation: AAA39735.1.
BC027210 mRNA. Translation: AAH27210.1.
BC046956 mRNA. Translation: AAH46956.1.
BC080811 mRNA. Translation: AAH80811.1.
M58585 mRNA. Translation: AAA39482.1.
CCDSiCCDS20493.1.
PIRiA40399.
RefSeqiNP_034879.2. NM_010749.6.
UniGeneiMm.249225.

3D structure databases

ProteinModelPortaliP24668.
SMRiP24668. Positions 29-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201263. 7 interactions.
IntActiP24668. 9 interactions.
MINTiMINT-4102408.
STRINGi10090.ENSMUSP00000007602.

PTM databases

iPTMnetiP24668.
PhosphoSiteiP24668.
SwissPalmiP24668.

Proteomic databases

EPDiP24668.
PaxDbiP24668.
PRIDEiP24668.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007602; ENSMUSP00000007602; ENSMUSG00000007458.
ENSMUST00000112610; ENSMUSP00000108229; ENSMUSG00000007458.
GeneIDi17113.
KEGGimmu:17113.
UCSCiuc009dow.1. mouse.

Organism-specific databases

CTDi4074.
MGIiMGI:96904. M6pr.

Phylogenomic databases

eggNOGiENOG410IK0D. Eukaryota.
ENOG4111GED. LUCA.
HOGENOMiHOG000013085.
HOVERGENiHBG006395.
InParanoidiP24668.
KOiK10089.
OMAiSDWIMLI.
OrthoDBiEOG7PP576.
PhylomeDBiP24668.
TreeFamiTF328910.

Enzyme and pathway databases

ReactomeiR-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

PROiP24668.
SOURCEiSearch...

Gene expression databases

BgeeiP24668.
CleanExiMM_M6PR.
ExpressionAtlasiP24668. baseline and differential.
GenevisibleiP24668. MM.

Family and domain databases

Gene3Di2.70.130.10. 1 hit.
InterProiIPR028927. Man-6-P_rcpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR000296. Man_6_P_rcpt.
[Graphical view]
PfamiPF02157. Man-6-P_recep. 1 hit.
[Graphical view]
PRINTSiPR00715. MAN6PRECEPTR.
SUPFAMiSSF50911. SSF50911. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gene and pseudogene of the mouse cation-dependent mannose 6-phosphate receptor. Genomic organization, expression, and chromosomal localization."
    Ludwig T., Ruether U., Metzger R., Copeland N.G., Jenkins N.A., Lobel P., Hoflack B.
    J. Biol. Chem. 267:12211-12219(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Molecular cloning of the mouse 46-kDa mannose 6-phosphate receptor (MPR 46)."
    Koester A., Nagel G., von Figura K., Pohlmann R.
    Biol. Chem. Hoppe-Seyler 372:297-300(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  3. "Cloning, sequencing, and functional characterization of the murine 46-kDa mannose 6-phosphate receptor."
    Ma Z., Grubb J.H., Sly W.S.
    J. Biol. Chem. 266:10589-10595(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland and Olfactory epithelium.
  5. "Differential regulation of mannose 6-phosphate receptors and their ligands during the myogenic development of C2 cells."
    Szebenyi G., Rotwein P.
    J. Biol. Chem. 266:5534-5539(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 156-233.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-84.
    Tissue: Myoblast.
  8. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-84.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiMPRD_MOUSE
AccessioniPrimary (citable) accession number: P24668
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 8, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This receptor has optimal binding in the presence of divalent cations.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.