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P24664

- TRYP_SACER

UniProt

P24664 - TRYP_SACER

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Protein

Trypsin

Gene
N/A
Organism
Saccharopolyspora erythraea (Streptomyces erythraeus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei42 – 421Charge relay system
Active sitei88 – 881Charge relay system
Sitei173 – 1731Required for specificityBy similarity
Active sitei179 – 1791Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.102.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin (EC:3.4.21.4)
Alternative name(s):
SET
OrganismiSaccharopolyspora erythraea (Streptomyces erythraeus)
Taxonomic identifieri1836 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227TrypsinPRO_0000088718Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 43
Disulfide bondi150 ↔ 164
Disulfide bondi175 ↔ 199

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

IntActiP24664. 1 interaction.
MINTiMINT-8289191.

Structurei

Secondary structure

1
227
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 113
Beta strandi15 – 195
Beta strandi25 – 339
Beta strandi36 – 394
Helixi41 – 444
Helixi49 – 513
Beta strandi53 – 575
Beta strandi66 – 7611
Helixi83 – 853
Beta strandi90 – 967
Helixi111 – 1133
Beta strandi118 – 12811
Beta strandi138 – 1447
Helixi147 – 1537
Turni159 – 1613
Beta strandi162 – 1654
Beta strandi182 – 1854
Beta strandi188 – 1958
Beta strandi197 – 2004
Beta strandi206 – 2105
Helixi211 – 2144
Helixi215 – 2228

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4M7GX-ray0.81A1-227[»]
ProteinModelPortaliP24664.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 223223Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24664-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
IVGGEDANVQ DHPFTVALVT PDGQQFCGGT LAAPNKVVTA AHCTVGSQPA
60 70 80 90 100
DINVVSGRTV MSSNIGTVSK VTNVWVHPEY QDAAKGFDVS VLTLEAPVKE
110 120 130 140 150
APIELAKADD AGYAPDTAAT ILGWGNTSEG GQQADHLQKA TVPVNSDDTC
160 170 180 190 200
KQAYGEYTPN AMVCAGVPEG GVDTCQGDSG GPMVVNNKLI GVTSWGEGCA
210 220
RPGKPGVYAR VGAYYDVLME QINAGAV
Length:227
Mass (Da):23,308
Last modified:March 1, 1992 - v1
Checksum:iD5AC5E47B227B418
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4M7G X-ray 0.81 A 1-227 [» ]
ProteinModelPortali P24664.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P24664. 1 interaction.
MINTi MINT-8289191.

Protein family/group databases

MEROPSi S01.102.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Miyamoto K., Matsuo H., Narita K.
    (In) Proceedings of Dai 30 kai tanpakushitsu kouzou touronkai kouen youshishuu, pp.77-80, Tokyo (1979)
    Cited for: PROTEIN SEQUENCE.
  2. "Crystal structure of Streptomyces erythraeus trypsin at 2.7-A resolution."
    Yamane T., Kobuke M., Tsutsui H., Toida T., Suzuki A., Ashida T., Kawata Y., Sakiyama F.
    J. Biochem. 110:945-950(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS), SEQUENCE REVISION.

Entry informationi

Entry nameiTRYP_SACER
AccessioniPrimary (citable) accession number: P24664
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: October 1, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Hardly autolyzes itself at all at its active pH range.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3