Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P24664 (TRYP_SACER) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trypsin

EC=3.4.21.4
Alternative name(s):
SET
OrganismSaccharopolyspora erythraea (Streptomyces erythraeus)
Taxonomic identifier1836 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Miscellaneous

Hardly autolyzes itself at all at its active pH range.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227Trypsin
PRO_0000088718

Regions

Domain1 – 223223Peptidase S1

Sites

Active site421Charge relay system
Active site881Charge relay system
Active site1791Charge relay system
Site1731Required for specificity By similarity

Amino acid modifications

Disulfide bond27 ↔ 43
Disulfide bond150 ↔ 164
Disulfide bond175 ↔ 199

Secondary structure

.......................................... 227
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P24664 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: D5AC5E47B227B418

FASTA22723,308
        10         20         30         40         50         60 
IVGGEDANVQ DHPFTVALVT PDGQQFCGGT LAAPNKVVTA AHCTVGSQPA DINVVSGRTV 

        70         80         90        100        110        120 
MSSNIGTVSK VTNVWVHPEY QDAAKGFDVS VLTLEAPVKE APIELAKADD AGYAPDTAAT 

       130        140        150        160        170        180 
ILGWGNTSEG GQQADHLQKA TVPVNSDDTC KQAYGEYTPN AMVCAGVPEG GVDTCQGDSG 

       190        200        210        220 
GPMVVNNKLI GVTSWGEGCA RPGKPGVYAR VGAYYDVLME QINAGAV 

« Hide

References

[1]Miyamoto K., Matsuo H., Narita K.
(In) Proceedings of Dai 30 kai tanpakushitsu kouzou touronkai kouen youshishuu, pp.77-80, Tokyo (1979)
Cited for: PROTEIN SEQUENCE.
[2]"Crystal structure of Streptomyces erythraeus trypsin at 2.7-A resolution."
Yamane T., Kobuke M., Tsutsui H., Toida T., Suzuki A., Ashida T., Kawata Y., Sakiyama F.
J. Biochem. 110:945-950(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS), SEQUENCE REVISION.

Cross-references

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4M7GX-ray0.81A1-227[»]
ProteinModelPortalP24664.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP24664. 1 interaction.
MINTMINT-8289191.

Protein family/group databases

MEROPSS01.102.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRYP_SACER
AccessionPrimary (citable) accession number: P24664
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references