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Protein

Trypsin

Gene
N/A
Organism
Saccharopolyspora erythraea (Streptomyces erythraeus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei42Charge relay system1
Active sitei88Charge relay system1
Sitei173Required for specificityBy similarity1
Active sitei179Charge relay system1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.102.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin (EC:3.4.21.4)
Alternative name(s):
SET
OrganismiSaccharopolyspora erythraea (Streptomyces erythraeus)
Taxonomic identifieri1836 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeSaccharopolyspora

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000887181 – 227TrypsinAdd BLAST227

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi27 ↔ 43
Disulfide bondi150 ↔ 164
Disulfide bondi175 ↔ 199

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

IntActiP24664. 1 interactor.
MINTiMINT-8289191.
STRINGi405948.SeryN2_010100013592.

Structurei

Secondary structure

1227
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Beta strandi15 – 19Combined sources5
Beta strandi25 – 33Combined sources9
Beta strandi36 – 39Combined sources4
Helixi41 – 44Combined sources4
Helixi49 – 51Combined sources3
Beta strandi53 – 57Combined sources5
Beta strandi66 – 76Combined sources11
Helixi83 – 85Combined sources3
Beta strandi90 – 96Combined sources7
Helixi111 – 113Combined sources3
Beta strandi118 – 128Combined sources11
Beta strandi138 – 144Combined sources7
Helixi147 – 153Combined sources7
Turni159 – 161Combined sources3
Beta strandi162 – 165Combined sources4
Beta strandi182 – 185Combined sources4
Beta strandi188 – 195Combined sources8
Beta strandi197 – 200Combined sources4
Beta strandi206 – 210Combined sources5
Helixi211 – 214Combined sources4
Helixi215 – 222Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4M7GX-ray0.81A1-227[»]
ProteinModelPortaliP24664.
SMRiP24664.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 223Peptidase S1PROSITE-ProRule annotationAdd BLAST223

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105NDH. Bacteria.
COG5640. LUCA.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24664-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
IVGGEDANVQ DHPFTVALVT PDGQQFCGGT LAAPNKVVTA AHCTVGSQPA
60 70 80 90 100
DINVVSGRTV MSSNIGTVSK VTNVWVHPEY QDAAKGFDVS VLTLEAPVKE
110 120 130 140 150
APIELAKADD AGYAPDTAAT ILGWGNTSEG GQQADHLQKA TVPVNSDDTC
160 170 180 190 200
KQAYGEYTPN AMVCAGVPEG GVDTCQGDSG GPMVVNNKLI GVTSWGEGCA
210 220
RPGKPGVYAR VGAYYDVLME QINAGAV
Length:227
Mass (Da):23,308
Last modified:March 1, 1992 - v1
Checksum:iD5AC5E47B227B418
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4M7GX-ray0.81A1-227[»]
ProteinModelPortaliP24664.
SMRiP24664.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24664. 1 interactor.
MINTiMINT-8289191.
STRINGi405948.SeryN2_010100013592.

Protein family/group databases

MEROPSiS01.102.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105NDH. Bacteria.
COG5640. LUCA.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRYP_SACER
AccessioniPrimary (citable) accession number: P24664
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Hardly autolyzes itself at all at its active pH range.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.