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Protein

Trypsin

Gene
N/A
Organism
Saccharopolyspora erythraea (Streptomyces erythraeus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei42 – 421Charge relay system
Active sitei88 – 881Charge relay system
Sitei173 – 1731Required for specificityBy similarity
Active sitei179 – 1791Charge relay system

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.102.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin (EC:3.4.21.4)
Alternative name(s):
SET
OrganismiSaccharopolyspora erythraea (Streptomyces erythraeus)
Taxonomic identifieri1836 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227TrypsinPRO_0000088718Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 43
Disulfide bondi150 ↔ 164
Disulfide bondi175 ↔ 199

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

IntActiP24664. 1 interaction.
MINTiMINT-8289191.

Structurei

Secondary structure

1
227
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 113Combined sources
Beta strandi15 – 195Combined sources
Beta strandi25 – 339Combined sources
Beta strandi36 – 394Combined sources
Helixi41 – 444Combined sources
Helixi49 – 513Combined sources
Beta strandi53 – 575Combined sources
Beta strandi66 – 7611Combined sources
Helixi83 – 853Combined sources
Beta strandi90 – 967Combined sources
Helixi111 – 1133Combined sources
Beta strandi118 – 12811Combined sources
Beta strandi138 – 1447Combined sources
Helixi147 – 1537Combined sources
Turni159 – 1613Combined sources
Beta strandi162 – 1654Combined sources
Beta strandi182 – 1854Combined sources
Beta strandi188 – 1958Combined sources
Beta strandi197 – 2004Combined sources
Beta strandi206 – 2105Combined sources
Helixi211 – 2144Combined sources
Helixi215 – 2228Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4M7GX-ray0.81A1-227[»]
ProteinModelPortaliP24664.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 223223Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24664-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
IVGGEDANVQ DHPFTVALVT PDGQQFCGGT LAAPNKVVTA AHCTVGSQPA
60 70 80 90 100
DINVVSGRTV MSSNIGTVSK VTNVWVHPEY QDAAKGFDVS VLTLEAPVKE
110 120 130 140 150
APIELAKADD AGYAPDTAAT ILGWGNTSEG GQQADHLQKA TVPVNSDDTC
160 170 180 190 200
KQAYGEYTPN AMVCAGVPEG GVDTCQGDSG GPMVVNNKLI GVTSWGEGCA
210 220
RPGKPGVYAR VGAYYDVLME QINAGAV
Length:227
Mass (Da):23,308
Last modified:March 1, 1992 - v1
Checksum:iD5AC5E47B227B418
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4M7GX-ray0.81A1-227[»]
ProteinModelPortaliP24664.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24664. 1 interaction.
MINTiMINT-8289191.

Protein family/group databases

MEROPSiS01.102.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Miyamoto K., Matsuo H., Narita K.
    (In) Proceedings of Dai 30 kai tanpakushitsu kouzou touronkai kouen youshishuu, pp.77-80, Tokyo (1979)
    Cited for: PROTEIN SEQUENCE.
  2. "Crystal structure of Streptomyces erythraeus trypsin at 2.7-A resolution."
    Yamane T., Kobuke M., Tsutsui H., Toida T., Suzuki A., Ashida T., Kawata Y., Sakiyama F.
    J. Biochem. 110:945-950(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS), SEQUENCE REVISION.

Entry informationi

Entry nameiTRYP_SACER
AccessioniPrimary (citable) accession number: P24664
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: May 27, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Hardly autolyzes itself at all at its active pH range.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.