P24658 (L_CDVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Canine distemper virus (strain Onderstepoort) (CDV) [Complete proteome]

Taxonomic identifier

11233 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Morbillivirus

Virus host

Canis familiaris (Dog) (Canis lupus familiaris) [TaxID: 9615]
Ailuropoda melanoleuca (Giant panda) [TaxID: 9646]
Ailurus fulgens (Lesser panda) (Red panda) [TaxID: 9649]
Procyon lotor (Raccoon) [TaxID: 9654]
Mustela [TaxID: 9665]
Panthera leo (Lion) [TaxID: 9689]
Zalophus californianus (California sealion) [TaxID: 9704]

Protein attributes

Sequence length	2184 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the paramyxovirus L protein family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2184	2184	RNA-directed RNA polymerase L		PRO_0000142726
Regions
	Domain	656 – 840	185	RdRp catalytic
	Nucleotide binding	1785 – 1794	10	ATP Potential
Experimental info
	Sequence conflict	44	1	T → R in BAA00655. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P24658 [UniParc]. Last modified May 30, 2000. Version 3. Checksum: BEF45756BBF0D37D	FASTA	2,184	248,190
	10 20 30 40 50 60 MDSVSVNQIL YPEVHLDSPI VTNKLVSILE YARIRHNYQL LDTTLVRNIK ERISEGFSNQ 70 80 90 100 110 120 MIINCIEIGS IINQTLLSYP KHNHVIYPNC NKLLFHAQDR VISLRLRNIF KRGNSIYSKI 130 140 150 160 170 180 TDGVKKCLND INLNIGLGGA LDKTIGTKID EAGIIMQSSQ WFEPFLLWFT IKTEMRSVIK 190 200 210 220 230 240 SSTHNCRKRR QNPVFVKGES LNVLVSRDLV CIIDLTSHIV YYLTFEMVLM YCDVIEGRLM 250 260 270 280 290 300 TDTAMAIDQR YSTLHVRIRY LWDLIDGFFP DLGNSTYQLV ALLEPLSLAY LQLKDITFSL 310 320 330 340 350 360 RGAFLSHCFA EIQEILQDNG FYTEETFQTL TQALDFVFIT EDIHITGEIF SFFRSFGHPR 370 380 390 400 410 420 LEAITAAENV RKHMNQPKVV SYETMMKGHA IFCGIIINGY RDRHGGTWPP MDLPVHASPI 430 440 450 460 470 480 IRNAHASGEG ITYSQCIENW KSFAGIRFKC FMPLSLDSDL TMYLKDKALA ALRKEWDSVY 490 500 510 520 530 540 PKEFLRYNPP RSTESRRLVN VFLEDSQFDP YNMIMYVISG QYLEDPDFNL SYSLKEKEIK 550 560 570 580 590 600 EVGRLFAKMT YKMRACQVIA ENLISNGIGK YFKDNGMAKD EHDLTKSLHT LAVSGVPKDK 610 620 630 640 650 660 KDSHRGLTNQ RKSLKPAPYR GTRHSVSSPS SRYIDPNPNF CTSRREDNDI EIYETVSAFI 670 680 690 700 710 720 TTDLKKYCLN WRYETISIFA QRLNEIYGLP SFFQWLHRRL EQSILYVSDP HCPPDLDRHV 730 740 750 760 770 780 DLNTAPNSQI FIKYPMGGVE GYCQKLWTIS TIPYLYLAAH ESGVRIASLV QGDNQTIAVT 790 800 810 820 830 840 KRVPSTWSYA LKKSEASRVT TEYFIALRQR LHDVGHHLKA NETIISSHFF VYSKGIYYDG 850 860 870 880 890 900 MLISQSLKSI ARCVFWSETI VDETRAACSN ISTTLAKAIE KGFDRYLAYT LNILKIIQQV 910 920 930 940 950 960 LISLGFTINS AMTRDVIEPL LQDHCLLTKM AILPAPIGGF NYLNMSRLFV RNIGDPVTSS 970 980 990 1000 1010 1020 IADLKRMIRS GLLGVEILHQ VMTQYPGDSS YLDWASDPYS ANLPCVQSIT RLLKNITARH 1030 1040 1050 1060 1070 1080 VLINSPNPML RGLFHDESQD EDEALAAFLM DRKIIIPRAA HEILDNTITG AREAIAGMLD 1090 1100 1110 1120 1130 1140 TTKGLIRASM KRGGLTPRII TRLSTYDYEQ FRAGIRLFSG KGHDQLIDQD SCSVQLARAL 1150 1160 1170 1180 1190 1200 RNHMWAKLAK GRPIYGLEVP DILESMKGYM IRRHESCLLC ASGSHNYGWF FIPANCQLDS 1210 1220 1230 1240 1250 1260 ITEGTSALRV PYIGSTTEER TDMKLAFVKS PSRSLKSAVR IATVYSWAYG DDDESWQEAW 1270 1280 1290 1300 1310 1320 TLAKQRADIS LEELRMITPI STSTNLAHRL RDKSTQVKYS GTSLIRVARY ATISNDNLSF 1330 1340 1350 1360 1370 1380 IIDDKKVDTN FIYQQGMLLG LGILEHLFRL SSTTGDSNTV LHLHVETDCC VIPMSDHPRV 1390 1400 1410 1420 1430 1440 PGLRKVVIPR NICTNPLIYD SNPIIEKDAV RLYNQSHRKH IVEFVTWTTG QLYHVLAKST 1450 1460 1470 1480 1490 1500 AMSMVEMITK FEKDHLNEVT ALIGDDDINS FITEFLLVEP RLFTVYLGQC AAINWGFEIH 1510 1520 1530 1540 1550 1560 YHRPSGKYQM GELLFSFLSR MSKGVFKILA NALSHPKVYR RFWDSGMIEP VHGPSLDSQN 1570 1580 1590 1600 1610 1620 LHITVCNLIY NCYMIYLDLL LNDELDDFSF ILCESDEDVI PERFDNIQAR HLCILSDLYC 1630 1640 1650 1660 1670 1680 NPRDCPQIRG LTPTQKCAVL SGYLKSKALE SHVGLTWNDK PILIDQYSCS LTYLRRGSIK 1690 1700 1710 1720 1730 1740 QIRLRVDPGF ITDAVGCLER RPLRNNSTSK ASELTSGFDP PKDDLAKLLS QLSTRTHNLP 1750 1760 1770 1780 1790 1800 ITGLGVRNYE VHSFRRIGIN STACYKAVEI ASVIKNEFTS EEHGLFLGEG SGAMLTVYKE 1810 1820 1830 1840 1850 1860 LLRLSRCYYN SGVSVESRTG QREISPYPSE VSLVEHQLGL DKLVTVLFNG RPEVTWVGSV 1870 1880 1890 1900 1910 1920 DCYKYILSQI SASSLGLIHS DIESLPDKDI IEKLEELSAI LSMTLILGKV GSVLVIKIMP 1930 1940 1950 1960 1970 1980 VSGDWVQGFI LYALPHFLRS FIVYPRYSNF VSTEAYLVFT GLRAGRLINP EGIKQQILRV 1990 2000 2010 2020 2030 2040 GIRTSPGLVG HILSSKQTAC VQSLHGPPFH AKSFNPHLQG LTSIEKVLIN CGLTINGLKV 2050 2060 2070 2080 2090 2100 CKNLLHHDIS SGEEGLKGSI TILYRELARF KDNHQSSHGM FHAYPVLIAS QERELVSIIA 2110 2120 2130 2140 2150 2160 KKYCGYILLY SGDLYEITRI VRNLKANHII FDLHRNLFMD NLSRSDRSLI LTTIPKKNWL 2170 2180 FQLETKEIKE WFKLLGYSAL IRNH

References

[1]	"Canine distemper virus L gene: sequence and comparison with related viruses." Sidhu M.S., Menonna J.P., Cook S.D., Dowling P.C., Udem S.A. Virology 193:50-65(1993) [PubMed: 8438585] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	Sidhu M.S. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"The nucleotide sequence of the gene encoding the attachment protein H of canine distemper virus." Curran M.D., Clarke D.K., Rima B.K. J. Gen. Virol. 72:443-447(1991) [PubMed: 1993883] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-63.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF014953 Genomic RNA. Translation: AAC26996.1. D00758 Genomic RNA. Translation: BAA00655.1.
PIR	A45389.
RefSeq	NP_047207.1. NC_001921.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1489795.
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. IPR016269. RNA-dir_pol_paramyxovirus. IPR024352. RNA_pol_cap_MeTfrase. [Graphical view]
Pfam	PF12803. G-7-MTase. 1 hit. PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000830. RNA_pol_ParamyxoV. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_CDVO

Accession

Primary (citable) accession number: P24658

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	May 30, 2000
Last modified:	December 14, 2011
This is version 69 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program