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P24638 (PPAL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Lysosomal acid phosphatase
Short name=LAP
EC=3.1.3.2
Gene names
Name:Acp2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Subcellular location

Lysosome membrane; Single-pass membrane protein; Lumenal side By similarity. Lysosome lumen By similarity. Note: The soluble form arises by proteolytic processing of the membrane-bound form By similarity.

Post-translational modification

The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen By similarity.

Sequence similarities

Belongs to the histidine acid phosphatase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030
Chain31 – 423393Lysosomal acid phosphatase
PRO_0000023961

Regions

Topological domain31 – 381351Lumenal Potential
Transmembrane382 – 40221Helical; Potential
Topological domain403 – 42321Cytoplasmic Potential

Sites

Active site421Nucleophile By similarity
Active site2871Proton donor By similarity

Amino acid modifications

Glycosylation921N-linked (GlcNAc...) Potential
Glycosylation1331N-linked (GlcNAc...) Potential
Glycosylation1671N-linked (GlcNAc...) Potential
Glycosylation1771N-linked (GlcNAc...) Potential
Glycosylation1911N-linked (GlcNAc...) Potential
Glycosylation1971N-linked (GlcNAc...) Potential
Glycosylation2671N-linked (GlcNAc...) Potential
Glycosylation3221N-linked (GlcNAc...) Potential
Glycosylation3311N-linked (GlcNAc...) Potential
Glycosylation3741N-linked (GlcNAc...) Potential
Disulfide bond159 ↔ 370 By similarity
Disulfide bond212 ↔ 310 By similarity
Disulfide bond345 ↔ 349 By similarity

Experimental info

Sequence conflict1311N → S in CAA40485. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P24638 [UniParc].

Last modified July 11, 2003. Version 2.
Checksum: AFD7A5C90A4F2AF4

FASTA42348,509
        10         20         30         40         50         60 
MAGRQTGWSQ AALLQFLLGM CLTVMPPIQA RSLRFVTLLY RHGDRSPVKT YPKDPYQEEK 

        70         80         90        100        110        120 
WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNTSYHRQEV YVRSTDFDRT LMSAEANLAG 

       130        140        150        160        170        180 
LFPPNEVQHF NPNISWQPIP VHTVPITEDR LLKFPLGPCP RYEQLQNETR QTPEYQNRSI 

       190        200        210        220        230        240 
QNAQFLNMVA NETGLTNVTL ETIWNVYDTL FCEQTHGLLL PPWASPQTVQ RLSQLKDFSF 

       250        260        270        280        290        300 
LFLFGIHEQV QKARLQGGVL LAQILKNLTL MATTSQFPKL LVYSAHDTTL VALQMALNVY 

       310        320        330        340        350        360 
NGKQAPYASC HIFELYQEDN GNFSVEMYFR NDSKKAPWPL ILPGCPHRCP LQDFLRLTEP 

       370        380        390        400        410        420 
VIPKDWQKEC QLANDTADTE VIVALAVCGS ILFLLIVLLL TILFRMQAQP PGYHHVADRE 


DHA

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[2]"Molecular cloning of the mouse lysosomal acid phosphatase."
Geier C., von Figura K., Pohlmann R.
Biol. Chem. Hoppe-Seyler 372:301-304(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-423.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC023343 mRNA. Translation: AAH23343.1.
X57199 mRNA. Translation: CAA40485.1.
IPIIPI00154056.
PIRS14742.
RefSeqNP_031413.1. NM_007387.2.
UniGeneMm.45570.

3D structure databases

ProteinModelPortalP24638.
SMRP24638. Positions 31-370.
ModBaseSearch...

PTM databases

PhosphoSiteP24638.

Proteomic databases

PaxDbP24638.
PRIDEP24638.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000002172; ENSMUSP00000002172; ENSMUSG00000002103.
GeneID11432.
KEGGmmu:11432.

Organism-specific databases

CTD53.
MGIMGI:87882. Acp2.

Phylogenomic databases

eggNOGNOG85977.
GeneTreeENSGT00530000062956.
HOGENOMHOG000231439.
HOVERGENHBG002203.
InParanoidP24638.
KOK14410.
OMACHMFELY.

Gene expression databases

ArrayExpressP24638.
BgeeP24638.
CleanExMM_ACP2.
GenevestigatorP24638.
GermOnlineENSMUSG00000002103. Mus musculus.

Family and domain databases

InterProIPR000560. His_Pase_superF_clade-2.
[Graphical view]
PfamPF00328. His_Phos_2. 1 hit.
[Graphical view]
PROSITEPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio278716.
SOURCESearch...

Entry information

Entry namePPAL_MOUSE
AccessionPrimary (citable) accession number: P24638
Secondary accession number(s): Q8QZT5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 11, 2003
Last modified: May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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