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Protein

Lysosomal acid phosphatase

Gene

Acp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei42 – 421NucleophileBy similarity
Active sitei287 – 2871Proton donorBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: MGI

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. lysosome organization Source: MGI
  3. skeletal system development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal acid phosphatase (EC:3.1.3.2)
Short name:
LAP
Gene namesi
Name:Acp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:87882. Acp2.

Subcellular locationi

Lysosome membrane By similarity; Single-pass membrane protein By similarity; Lumenal side By similarity. Lysosome lumen By similarity
Note: The soluble form arises by proteolytic processing of the membrane-bound form.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini31 – 381351LumenalSequence AnalysisAdd
BLAST
Transmembranei382 – 40221HelicalSequence AnalysisAdd
BLAST
Topological domaini403 – 42321CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. lysosomal lumen Source: UniProtKB-SubCell
  4. lysosomal membrane Source: UniProtKB-SubCell
  5. lysosome Source: MGI
  6. membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Add
BLAST
Chaini31 – 423393Lysosomal acid phosphatasePRO_0000023961Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi159 ↔ 370By similarity
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi212 ↔ 310By similarity
Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi345 ↔ 349By similarity
Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP24638.
PaxDbiP24638.
PRIDEiP24638.

PTM databases

PhosphoSiteiP24638.

Expressioni

Gene expression databases

BgeeiP24638.
CleanExiMM_ACP2.
ExpressionAtlasiP24638. baseline and differential.
GenevestigatoriP24638.

Structurei

3D structure databases

ProteinModelPortaliP24638.
SMRiP24638. Positions 31-370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG85977.
GeneTreeiENSGT00530000062956.
HOGENOMiHOG000231439.
HOVERGENiHBG002203.
InParanoidiP24638.
KOiK14410.
OMAiQDFLHLT.
PhylomeDBiP24638.
TreeFamiTF312893.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24638-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRQTGWSQ AALLQFLLGM CLTVMPPIQA RSLRFVTLLY RHGDRSPVKT
60 70 80 90 100
YPKDPYQEEK WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNTSYHRQEV
110 120 130 140 150
YVRSTDFDRT LMSAEANLAG LFPPNEVQHF NPNISWQPIP VHTVPITEDR
160 170 180 190 200
LLKFPLGPCP RYEQLQNETR QTPEYQNRSI QNAQFLNMVA NETGLTNVTL
210 220 230 240 250
ETIWNVYDTL FCEQTHGLLL PPWASPQTVQ RLSQLKDFSF LFLFGIHEQV
260 270 280 290 300
QKARLQGGVL LAQILKNLTL MATTSQFPKL LVYSAHDTTL VALQMALNVY
310 320 330 340 350
NGKQAPYASC HIFELYQEDN GNFSVEMYFR NDSKKAPWPL ILPGCPHRCP
360 370 380 390 400
LQDFLRLTEP VIPKDWQKEC QLANDTADTE VIVALAVCGS ILFLLIVLLL
410 420
TILFRMQAQP PGYHHVADRE DHA
Length:423
Mass (Da):48,509
Last modified:July 11, 2003 - v2
Checksum:iAFD7A5C90A4F2AF4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti131 – 1311N → S in CAA40485 (PubMed:2059337).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC023343 mRNA. Translation: AAH23343.1.
X57199 mRNA. Translation: CAA40485.1.
CCDSiCCDS16427.1.
PIRiS14742.
RefSeqiNP_031413.1. NM_007387.2.
UniGeneiMm.45570.

Genome annotation databases

EnsembliENSMUST00000002172; ENSMUSP00000002172; ENSMUSG00000002103.
GeneIDi11432.
KEGGimmu:11432.
UCSCiuc008kvf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC023343 mRNA. Translation: AAH23343.1.
X57199 mRNA. Translation: CAA40485.1.
CCDSiCCDS16427.1.
PIRiS14742.
RefSeqiNP_031413.1. NM_007387.2.
UniGeneiMm.45570.

3D structure databases

ProteinModelPortaliP24638.
SMRiP24638. Positions 31-370.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiP24638.

Proteomic databases

MaxQBiP24638.
PaxDbiP24638.
PRIDEiP24638.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002172; ENSMUSP00000002172; ENSMUSG00000002103.
GeneIDi11432.
KEGGimmu:11432.
UCSCiuc008kvf.2. mouse.

Organism-specific databases

CTDi53.
MGIiMGI:87882. Acp2.

Phylogenomic databases

eggNOGiNOG85977.
GeneTreeiENSGT00530000062956.
HOGENOMiHOG000231439.
HOVERGENiHBG002203.
InParanoidiP24638.
KOiK14410.
OMAiQDFLHLT.
PhylomeDBiP24638.
TreeFamiTF312893.

Miscellaneous databases

ChiTaRSiAcp2. mouse.
NextBioi278716.
PROiP24638.
SOURCEiSearch...

Gene expression databases

BgeeiP24638.
CleanExiMM_ACP2.
ExpressionAtlasiP24638. baseline and differential.
GenevestigatoriP24638.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  2. "Molecular cloning of the mouse lysosomal acid phosphatase."
    Geier C., von Figura K., Pohlmann R.
    Biol. Chem. Hoppe-Seyler 372:301-304(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-423.

Entry informationi

Entry nameiPPAL_MOUSE
AccessioniPrimary (citable) accession number: P24638
Secondary accession number(s): Q8QZT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 11, 2003
Last modified: February 4, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.