Reviewed, UniProtKB/Swiss-Prot P24638 (PPAL_MOUSE)

Names and origin

Protein names

Recommended name:
    Lysosomal acid phosphatase
      Short name=LAP
    EC=3.1.3.2

Gene names

Name:

Acp2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Subcellular location	Lysosome membrane; Single-pass membrane protein; Lumenal side By similarity. Lysosome lumen By similarity. Note: The soluble form arises by proteolytic processing of the membrane-bound form By similarity.
Post-translational modification	The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen By similarity.
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Cellular component	Lysosome Membrane
Domain	Signal Transmembrane
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	lysosome organization Inferred from mutant phenotype. Source: MGI skeletal system development Inferred from mutant phenotype. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosomal lumen Inferred from electronic annotation. Source: UniProtKB-SubCell lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acid phosphatase activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30
	Chain	31 – 423	393	Lysosomal acid phosphatase		PRO_0000023961
Regions
	Topological domain	31 – 381	351	Lumenal Potential
	Transmembrane	382 – 402	21	Potential
	Topological domain	403 – 423	21	Cytoplasmic Potential
Sites
	Active site	42	1	Nucleophile By similarity
	Active site	287	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
	Glycosylation	177	1	N-linked (GlcNAc...) Potential
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Glycosylation	267	1	N-linked (GlcNAc...) Potential
	Glycosylation	322	1	N-linked (GlcNAc...) Potential
	Glycosylation	331	1	N-linked (GlcNAc...) Potential
	Glycosylation	374	1	N-linked (GlcNAc...) Potential
	Disulfide bond	159 ↔ 370		By similarity
	Disulfide bond	212 ↔ 310		By similarity
	Disulfide bond	345 ↔ 349		By similarity
Experimental info
	Sequence conflict	131	1	N → S in CAA40485. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P24638-1 [UniParc]. Last modified July 11, 2003. Version 2. Checksum: AFD7A5C90A4F2AF4	FASTA	423	48,509
	10 20 30 40 50 60 MAGRQTGWSQ AALLQFLLGM CLTVMPPIQA RSLRFVTLLY RHGDRSPVKT YPKDPYQEEK 70 80 90 100 110 120 WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNTSYHRQEV YVRSTDFDRT LMSAEANLAG 130 140 150 160 170 180 LFPPNEVQHF NPNISWQPIP VHTVPITEDR LLKFPLGPCP RYEQLQNETR QTPEYQNRSI 190 200 210 220 230 240 QNAQFLNMVA NETGLTNVTL ETIWNVYDTL FCEQTHGLLL PPWASPQTVQ RLSQLKDFSF 250 260 270 280 290 300 LFLFGIHEQV QKARLQGGVL LAQILKNLTL MATTSQFPKL LVYSAHDTTL VALQMALNVY 310 320 330 340 350 360 NGKQAPYASC HIFELYQEDN GNFSVEMYFR NDSKKAPWPL ILPGCPHRCP LQDFLRLTEP 370 380 390 400 410 420 VIPKDWQKEC QLANDTADTE VIVALAVCGS ILFLLIVLLL TILFRMQAQP PGYHHVADRE DHA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[2]	"Molecular cloning of the mouse lysosomal acid phosphatase." Geier C., von Figura K., Pohlmann R. Biol. Chem. Hoppe-Seyler 372:301-304(1991) [PubMed: 2059337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-423.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC023343 mRNA. Translation: AAH23343.1. X57199 mRNA. Translation: CAA40485.1.
IPI	IPI00154056.
PIR	S14742.
RefSeq	NP_031413.1.
UniGene	Mm.45570
3D structure databases
HSSP	HSSP built from PDB template 1ND5 based on UniProtKB P15309.
ModBase	Search...
Proteomic databases
PRIDE	P24638.
Genome annotation databases
Ensembl	ENSMUSG00000002103. Mus musculus. [Contig view]
GeneID	11432.
KEGG	mmu:11432.
Organism-specific databases
MGI	MGI:87882. Acp2.
Phylogenomic databases
HOGENOM	P24638.
HOVERGEN	P24638.
OMA	P24638. ELYQEDS.
Enzyme and pathway databases
BRENDA	3.1.3.2. 244.
Gene expression databases
ArrayExpress	P24638.
Bgee	P24638.
CleanEx	MM_ACP2.
GermOnline	ENSMUSG00000002103. Mus musculus.
Family and domain databases
InterPro	IPR000560. Histidine_acid_Pase. [Graphical view]
Pfam	PF00328. Acid_phosphat_A. 1 hit. [Graphical view]
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	278716.
SOURCE	Search...

Entry information

Entry name

PPAL_MOUSE

Accession

Primary (citable) accession number: P24638
Secondary accession number(s): Q8QZT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	July 11, 2003
Last modified:	June 16, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)