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P24638

- PPAL_MOUSE

UniProt

P24638 - PPAL_MOUSE

Protein

Lysosomal acid phosphatase

Gene

Acp2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (11 Jul 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei42 – 421NucleophileBy similarity
    Active sitei287 – 2871Proton donorBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: MGI

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. lysosome organization Source: MGI
    3. skeletal system development Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysosomal acid phosphatase (EC:3.1.3.2)
    Short name:
    LAP
    Gene namesi
    Name:Acp2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:87882. Acp2.

    Subcellular locationi

    Lysosome membrane By similarity; Single-pass membrane protein By similarity; Lumenal side By similarity. Lysosome lumen By similarity
    Note: The soluble form arises by proteolytic processing of the membrane-bound form.By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. lysosomal lumen Source: UniProtKB-SubCell
    3. lysosomal membrane Source: UniProtKB-SubCell
    4. lysosome Source: MGI

    Keywords - Cellular componenti

    Lysosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Add
    BLAST
    Chaini31 – 423393Lysosomal acid phosphatasePRO_0000023961Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi159 ↔ 370By similarity
    Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi212 ↔ 310By similarity
    Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi345 ↔ 349By similarity
    Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP24638.
    PRIDEiP24638.

    PTM databases

    PhosphoSiteiP24638.

    Expressioni

    Gene expression databases

    ArrayExpressiP24638.
    BgeeiP24638.
    CleanExiMM_ACP2.
    GenevestigatoriP24638.

    Structurei

    3D structure databases

    ProteinModelPortaliP24638.
    SMRiP24638. Positions 31-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini31 – 381351LumenalSequence AnalysisAdd
    BLAST
    Topological domaini403 – 42321CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei382 – 40221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the histidine acid phosphatase family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG85977.
    GeneTreeiENSGT00530000062956.
    HOGENOMiHOG000231439.
    HOVERGENiHBG002203.
    InParanoidiP24638.
    KOiK14410.
    OMAiQDFLHLT.
    PhylomeDBiP24638.
    TreeFamiTF312893.

    Family and domain databases

    Gene3Di3.40.50.1240. 1 hit.
    InterProiIPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    [Graphical view]
    PfamiPF00328. His_Phos_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53254. SSF53254. 1 hit.
    PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
    PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24638-1 [UniParc]FASTAAdd to Basket

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    MAGRQTGWSQ AALLQFLLGM CLTVMPPIQA RSLRFVTLLY RHGDRSPVKT    50
    YPKDPYQEEK WPQGFGQLTK EGMLQHWELG QALRQRYHGF LNTSYHRQEV 100
    YVRSTDFDRT LMSAEANLAG LFPPNEVQHF NPNISWQPIP VHTVPITEDR 150
    LLKFPLGPCP RYEQLQNETR QTPEYQNRSI QNAQFLNMVA NETGLTNVTL 200
    ETIWNVYDTL FCEQTHGLLL PPWASPQTVQ RLSQLKDFSF LFLFGIHEQV 250
    QKARLQGGVL LAQILKNLTL MATTSQFPKL LVYSAHDTTL VALQMALNVY 300
    NGKQAPYASC HIFELYQEDN GNFSVEMYFR NDSKKAPWPL ILPGCPHRCP 350
    LQDFLRLTEP VIPKDWQKEC QLANDTADTE VIVALAVCGS ILFLLIVLLL 400
    TILFRMQAQP PGYHHVADRE DHA 423
    Length:423
    Mass (Da):48,509
    Last modified:July 11, 2003 - v2
    Checksum:iAFD7A5C90A4F2AF4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti131 – 1311N → S in CAA40485. (PubMed:2059337)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC023343 mRNA. Translation: AAH23343.1.
    X57199 mRNA. Translation: CAA40485.1.
    CCDSiCCDS16427.1.
    PIRiS14742.
    RefSeqiNP_031413.1. NM_007387.2.
    UniGeneiMm.45570.

    Genome annotation databases

    EnsembliENSMUST00000002172; ENSMUSP00000002172; ENSMUSG00000002103.
    GeneIDi11432.
    KEGGimmu:11432.
    UCSCiuc008kvf.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC023343 mRNA. Translation: AAH23343.1 .
    X57199 mRNA. Translation: CAA40485.1 .
    CCDSi CCDS16427.1.
    PIRi S14742.
    RefSeqi NP_031413.1. NM_007387.2.
    UniGenei Mm.45570.

    3D structure databases

    ProteinModelPortali P24638.
    SMRi P24638. Positions 31-370.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P24638.

    Proteomic databases

    PaxDbi P24638.
    PRIDEi P24638.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002172 ; ENSMUSP00000002172 ; ENSMUSG00000002103 .
    GeneIDi 11432.
    KEGGi mmu:11432.
    UCSCi uc008kvf.2. mouse.

    Organism-specific databases

    CTDi 53.
    MGIi MGI:87882. Acp2.

    Phylogenomic databases

    eggNOGi NOG85977.
    GeneTreei ENSGT00530000062956.
    HOGENOMi HOG000231439.
    HOVERGENi HBG002203.
    InParanoidi P24638.
    KOi K14410.
    OMAi QDFLHLT.
    PhylomeDBi P24638.
    TreeFami TF312893.

    Miscellaneous databases

    NextBioi 278716.
    PROi P24638.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P24638.
    Bgeei P24638.
    CleanExi MM_ACP2.
    Genevestigatori P24638.

    Family and domain databases

    Gene3Di 3.40.50.1240. 1 hit.
    InterProi IPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    [Graphical view ]
    Pfami PF00328. His_Phos_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53254. SSF53254. 1 hit.
    PROSITEi PS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
    PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    2. "Molecular cloning of the mouse lysosomal acid phosphatase."
      Geier C., von Figura K., Pohlmann R.
      Biol. Chem. Hoppe-Seyler 372:301-304(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-423.

    Entry informationi

    Entry nameiPPAL_MOUSE
    AccessioniPrimary (citable) accession number: P24638
    Secondary accession number(s): Q8QZT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: July 11, 2003
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3