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P24636

- TBB4_ARATH

UniProt

P24636 - TBB4_ARATH

Protein

Tubulin beta-4 chain

Gene

TUBB4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (10 Jan 2003)
      Previous versions | rss
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    Functioni

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi140 – 1467GTPSequence Analysis

    GO - Molecular functioni

    1. GTPase activity Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. structural constituent of cytoskeleton Source: TAIR

    GO - Biological processi

    1. microtubule-based process Source: InterPro
    2. protein polymerization Source: InterPro

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_216620. Post-chaperonin tubulin folding pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tubulin beta-4 chain
    Alternative name(s):
    Beta-4-tubulin
    Gene namesi
    Name:TUBB4
    Synonyms:TUB4
    Ordered Locus Names:At5g44340
    ORF Names:K9L2.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G44340.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. cell wall Source: TAIR
    3. chloroplast stroma Source: TAIR
    4. cytosol Source: TAIR
    5. Golgi apparatus Source: TAIR
    6. microtubule Source: UniProtKB-KW
    7. plasma membrane Source: TAIR
    8. plasmodesma Source: TAIR
    9. tubulin complex Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 444444Tubulin beta-4 chainPRO_0000048323Add
    BLAST

    Proteomic databases

    PaxDbiP24636.
    PRIDEiP24636.

    Expressioni

    Gene expression databases

    GenevestigatoriP24636.

    Interactioni

    Subunit structurei

    Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

    Protein-protein interaction databases

    BioGridi19709. 3 interactions.
    IntActiP24636. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP24636.
    SMRiP24636. Positions 2-427.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tubulin family.Curated

    Phylogenomic databases

    eggNOGiCOG5023.
    HOGENOMiHOG000165710.
    InParanoidiP24636.
    KOiK07375.
    OMAiTPPPHIF.
    PhylomeDBiP24636.

    Family and domain databases

    Gene3Di1.10.287.600. 1 hit.
    3.30.1330.20. 1 hit.
    3.40.50.1440. 1 hit.
    InterProiIPR013838. Beta-tubulin_BS.
    IPR002453. Beta_tubulin.
    IPR008280. Tub_FtsZ_C.
    IPR000217. Tubulin.
    IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
    IPR023123. Tubulin_C.
    IPR017975. Tubulin_CS.
    IPR003008. Tubulin_FtsZ_GTPase.
    [Graphical view]
    PANTHERiPTHR11588. PTHR11588. 1 hit.
    PfamiPF00091. Tubulin. 1 hit.
    PF03953. Tubulin_C. 1 hit.
    [Graphical view]
    PRINTSiPR01163. BETATUBULIN.
    PR01161. TUBULIN.
    SMARTiSM00864. Tubulin. 1 hit.
    SM00865. Tubulin_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52490. SSF52490. 1 hit.
    SSF55307. SSF55307. 1 hit.
    PROSITEiPS00227. TUBULIN. 1 hit.
    PS00228. TUBULIN_B_AUTOREG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24636-1 [UniParc]FASTAAdd to Basket

    « Hide

    MREILHIQGG QCGNQIGAKF WEVICDEHGI DHTGQYVGDS PLQLERIDVY    50
    FNEASGGKYV PRAVLMDLEP GTMDSLRSGP FGQIFRPDNF VFGQSGAGNN 100
    WAKGHYTEGA ELIDSVLDVV RKEAENSDCL QGFQVCHSLG GGTGSGMGTL 150
    LISKIREEYP DRMMMTFSVF PSPKVSDTVV EPYNATLSVH QLVENADECM 200
    VLDNEALYDI CFRTLKLANP TFGDLNHLIS ATMSGVTCCL RFPGQLNSDL 250
    RKLAVNLIPF PRLHFFMVGF APLTSRGSQQ YSALSVPELT QQMWDAKNMM 300
    CAADPRHGRY LTASAVFRGK LSTKEVDEQM MNIQNKNSSY FVEWIPNNVK 350
    SSVCDIAPKG LKMASTFIGN STSIQEMFRR VSEQFTAMFR RKAFLHWYTG 400
    EGMDEMEFTE AESNMNDLVA EYQQYQDATA GEEEYEEEEE EYET 444
    Length:444
    Mass (Da):49,823
    Last modified:January 10, 2003 - v2
    Checksum:iEEDC37FE26D1E80D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti313 – 3131A → R in AAA32757. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M21415 Genomic DNA. Translation: AAA32757.1.
    AB011475 Genomic DNA. Translation: BAB10119.1.
    CP002688 Genomic DNA. Translation: AED95098.1.
    AY059075 mRNA. Translation: AAL15181.1.
    AY035141 mRNA. Translation: AAK59645.1.
    PIRiS68122.
    RefSeqiNP_199247.1. NM_123801.1.
    UniGeneiAt.47572.
    At.66973.
    At.70628.
    At.74833.

    Genome annotation databases

    EnsemblPlantsiAT5G44340.1; AT5G44340.1; AT5G44340.
    GeneIDi834459.
    KEGGiath:AT5G44340.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M21415 Genomic DNA. Translation: AAA32757.1 .
    AB011475 Genomic DNA. Translation: BAB10119.1 .
    CP002688 Genomic DNA. Translation: AED95098.1 .
    AY059075 mRNA. Translation: AAL15181.1 .
    AY035141 mRNA. Translation: AAK59645.1 .
    PIRi S68122.
    RefSeqi NP_199247.1. NM_123801.1.
    UniGenei At.47572.
    At.66973.
    At.70628.
    At.74833.

    3D structure databases

    ProteinModelPortali P24636.
    SMRi P24636. Positions 2-427.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 19709. 3 interactions.
    IntActi P24636. 3 interactions.

    Proteomic databases

    PaxDbi P24636.
    PRIDEi P24636.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G44340.1 ; AT5G44340.1 ; AT5G44340 .
    GeneIDi 834459.
    KEGGi ath:AT5G44340.

    Organism-specific databases

    TAIRi AT5G44340.

    Phylogenomic databases

    eggNOGi COG5023.
    HOGENOMi HOG000165710.
    InParanoidi P24636.
    KOi K07375.
    OMAi TPPPHIF.
    PhylomeDBi P24636.

    Enzyme and pathway databases

    Reactomei REACT_216620. Post-chaperonin tubulin folding pathway.

    Gene expression databases

    Genevestigatori P24636.

    Family and domain databases

    Gene3Di 1.10.287.600. 1 hit.
    3.30.1330.20. 1 hit.
    3.40.50.1440. 1 hit.
    InterProi IPR013838. Beta-tubulin_BS.
    IPR002453. Beta_tubulin.
    IPR008280. Tub_FtsZ_C.
    IPR000217. Tubulin.
    IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
    IPR023123. Tubulin_C.
    IPR017975. Tubulin_CS.
    IPR003008. Tubulin_FtsZ_GTPase.
    [Graphical view ]
    PANTHERi PTHR11588. PTHR11588. 1 hit.
    Pfami PF00091. Tubulin. 1 hit.
    PF03953. Tubulin_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01163. BETATUBULIN.
    PR01161. TUBULIN.
    SMARTi SM00864. Tubulin. 1 hit.
    SM00865. Tubulin_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52490. SSF52490. 1 hit.
    SSF55307. SSF55307. 1 hit.
    PROSITEi PS00227. TUBULIN. 1 hit.
    PS00228. TUBULIN_B_AUTOREG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The relatively large beta-tubulin gene family of Arabidopsis contains a member with an unusual transcribed 5' noncoding sequence."
      Marks M.D., West J., Weeks D.P.
      Plant Mol. Biol. 10:91-104(1987)
      [AGRICOLA] [Europe PMC]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
      Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiTBB4_ARATH
    AccessioniPrimary (citable) accession number: P24636
    Secondary accession number(s): Q9FKV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: January 10, 2003
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are nine genes coding for beta-tubulin.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3