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Protein

Tubulin beta-4 chain

Gene

TUBB4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi140 – 1467GTPSequence Analysis

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_277547. Prefoldin mediated transfer of substrate to CCT/TriC.
REACT_304635. Formation of tubulin folding intermediates by CCT/TriC.
REACT_306784. Post-chaperonin tubulin folding pathway.
REACT_342512. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin beta-4 chain
Alternative name(s):
Beta-4-tubulin
Gene namesi
Name:TUBB4
Synonyms:TUB4
Ordered Locus Names:At5g44340
ORF Names:K9L2.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G44340.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • cell wall Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytosol Source: TAIR
  • Golgi apparatus Source: TAIR
  • microtubule Source: UniProtKB-KW
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • tubulin complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Tubulin beta-4 chainPRO_0000048323Add
BLAST

Proteomic databases

PaxDbiP24636.
PRIDEiP24636.

Interactioni

Subunit structurei

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

Protein-protein interaction databases

BioGridi19709. 4 interactions.
IntActiP24636. 3 interactions.
STRINGi3702.AT5G44340.1.

Structurei

3D structure databases

ProteinModelPortaliP24636.
SMRiP24636. Positions 2-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tubulin family.Curated

Phylogenomic databases

eggNOGiCOG5023.
HOGENOMiHOG000165710.
InParanoidiP24636.
KOiK07375.
OMAiWETICDE.
PhylomeDBiP24636.

Family and domain databases

Gene3Di1.10.287.600. 1 hit.
3.30.1330.20. 1 hit.
3.40.50.1440. 1 hit.
InterProiIPR013838. Beta-tubulin_BS.
IPR002453. Beta_tubulin.
IPR008280. Tub_FtsZ_C.
IPR000217. Tubulin.
IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
IPR023123. Tubulin_C.
IPR017975. Tubulin_CS.
IPR003008. Tubulin_FtsZ_GTPase.
[Graphical view]
PANTHERiPTHR11588. PTHR11588. 1 hit.
PfamiPF00091. Tubulin. 1 hit.
PF03953. Tubulin_C. 1 hit.
[Graphical view]
PRINTSiPR01163. BETATUBULIN.
PR01161. TUBULIN.
SMARTiSM00864. Tubulin. 1 hit.
SM00865. Tubulin_C. 1 hit.
[Graphical view]
SUPFAMiSSF52490. SSF52490. 1 hit.
SSF55307. SSF55307. 1 hit.
PROSITEiPS00227. TUBULIN. 1 hit.
PS00228. TUBULIN_B_AUTOREG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24636-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREILHIQGG QCGNQIGAKF WEVICDEHGI DHTGQYVGDS PLQLERIDVY
60 70 80 90 100
FNEASGGKYV PRAVLMDLEP GTMDSLRSGP FGQIFRPDNF VFGQSGAGNN
110 120 130 140 150
WAKGHYTEGA ELIDSVLDVV RKEAENSDCL QGFQVCHSLG GGTGSGMGTL
160 170 180 190 200
LISKIREEYP DRMMMTFSVF PSPKVSDTVV EPYNATLSVH QLVENADECM
210 220 230 240 250
VLDNEALYDI CFRTLKLANP TFGDLNHLIS ATMSGVTCCL RFPGQLNSDL
260 270 280 290 300
RKLAVNLIPF PRLHFFMVGF APLTSRGSQQ YSALSVPELT QQMWDAKNMM
310 320 330 340 350
CAADPRHGRY LTASAVFRGK LSTKEVDEQM MNIQNKNSSY FVEWIPNNVK
360 370 380 390 400
SSVCDIAPKG LKMASTFIGN STSIQEMFRR VSEQFTAMFR RKAFLHWYTG
410 420 430 440
EGMDEMEFTE AESNMNDLVA EYQQYQDATA GEEEYEEEEE EYET
Length:444
Mass (Da):49,823
Last modified:January 10, 2003 - v2
Checksum:iEEDC37FE26D1E80D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti313 – 3131A → R in AAA32757 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21415 Genomic DNA. Translation: AAA32757.1.
AB011475 Genomic DNA. Translation: BAB10119.1.
CP002688 Genomic DNA. Translation: AED95098.1.
AY059075 mRNA. Translation: AAL15181.1.
AY035141 mRNA. Translation: AAK59645.1.
PIRiS68122.
RefSeqiNP_199247.1. NM_123801.1.
UniGeneiAt.47572.
At.66973.
At.70628.
At.74833.

Genome annotation databases

EnsemblPlantsiAT5G44340.1; AT5G44340.1; AT5G44340.
GeneIDi834459.
KEGGiath:AT5G44340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21415 Genomic DNA. Translation: AAA32757.1.
AB011475 Genomic DNA. Translation: BAB10119.1.
CP002688 Genomic DNA. Translation: AED95098.1.
AY059075 mRNA. Translation: AAL15181.1.
AY035141 mRNA. Translation: AAK59645.1.
PIRiS68122.
RefSeqiNP_199247.1. NM_123801.1.
UniGeneiAt.47572.
At.66973.
At.70628.
At.74833.

3D structure databases

ProteinModelPortaliP24636.
SMRiP24636. Positions 2-427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19709. 4 interactions.
IntActiP24636. 3 interactions.
STRINGi3702.AT5G44340.1.

Proteomic databases

PaxDbiP24636.
PRIDEiP24636.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G44340.1; AT5G44340.1; AT5G44340.
GeneIDi834459.
KEGGiath:AT5G44340.

Organism-specific databases

TAIRiAT5G44340.

Phylogenomic databases

eggNOGiCOG5023.
HOGENOMiHOG000165710.
InParanoidiP24636.
KOiK07375.
OMAiWETICDE.
PhylomeDBiP24636.

Enzyme and pathway databases

ReactomeiREACT_277547. Prefoldin mediated transfer of substrate to CCT/TriC.
REACT_304635. Formation of tubulin folding intermediates by CCT/TriC.
REACT_306784. Post-chaperonin tubulin folding pathway.
REACT_342512. Kinesins.

Miscellaneous databases

PROiP24636.

Family and domain databases

Gene3Di1.10.287.600. 1 hit.
3.30.1330.20. 1 hit.
3.40.50.1440. 1 hit.
InterProiIPR013838. Beta-tubulin_BS.
IPR002453. Beta_tubulin.
IPR008280. Tub_FtsZ_C.
IPR000217. Tubulin.
IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
IPR023123. Tubulin_C.
IPR017975. Tubulin_CS.
IPR003008. Tubulin_FtsZ_GTPase.
[Graphical view]
PANTHERiPTHR11588. PTHR11588. 1 hit.
PfamiPF00091. Tubulin. 1 hit.
PF03953. Tubulin_C. 1 hit.
[Graphical view]
PRINTSiPR01163. BETATUBULIN.
PR01161. TUBULIN.
SMARTiSM00864. Tubulin. 1 hit.
SM00865. Tubulin_C. 1 hit.
[Graphical view]
SUPFAMiSSF52490. SSF52490. 1 hit.
SSF55307. SSF55307. 1 hit.
PROSITEiPS00227. TUBULIN. 1 hit.
PS00228. TUBULIN_B_AUTOREG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The relatively large beta-tubulin gene family of Arabidopsis contains a member with an unusual transcribed 5' noncoding sequence."
    Marks M.D., West J., Weeks D.P.
    Plant Mol. Biol. 10:91-104(1987)
    [AGRICOLA] [Europe PMC]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
    Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiTBB4_ARATH
AccessioniPrimary (citable) accession number: P24636
Secondary accession number(s): Q9FKV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 10, 2003
Last modified: June 24, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are nine genes coding for beta-tubulin.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.