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Protein

Chitinase 3

Gene

Cht3

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes chitin and plays a role in defense against fungal pathogens containing chitin. Inhibits the growth of T.reesei fungus on plate assay.2 Publications

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  • beta-N-acetylglucosaminidase activity Source: Gramene
  • chitinase activity Source: UniProtKB
  • chitin binding Source: Gramene
  • endochitinase activity Source: Gramene

GO - Biological processi

  • amino sugar metabolic process Source: Gramene
  • cell wall macromolecule catabolic process Source: InterPro
  • chitin catabolic process Source: UniProtKB-KW
  • defense response to fungus Source: UniProtKB
  • polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Enzyme and pathway databases

BRENDAi3.2.1.14. 4460.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase 3 (EC:3.2.1.14)
Alternative name(s):
Basic endochitinase 1
Class I chitinase c
Short name:
OsChia1c
Pathogenesis related (PR)-3 chitinase 3
Gene namesi
Name:Cht3
Synonyms:CH6
Ordered Locus Names:Os06g0726100, LOC_Os06g51050
ORF Names:P0017G10.2, P0548E04.22
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
ProteomesiUP000000763 Componenti: Chromosome 6

Organism-specific databases

GrameneiP24626.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 320302Chitinase 3PRO_0000005305Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi21 ↔ 36PROSITE-ProRule annotation
Disulfide bondi30 ↔ 42PROSITE-ProRule annotation
Disulfide bondi33 ↔ 61PROSITE-ProRule annotation
Disulfide bondi35 ↔ 49PROSITE-ProRule annotation
Disulfide bondi53 ↔ 57PROSITE-ProRule annotation
Disulfide bondi97 ↔ 159PROSITE-ProRule annotation
Disulfide bondi172 ↔ 180PROSITE-ProRule annotation
Disulfide bondi279 ↔ 311PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed at low levels in roots, leaves, sheaths and meristems.1 Publication

Gene expression databases

ExpressionAtlasiP24626. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os06g51050.1.

Structurei

3D structure databases

ProteinModelPortaliP24626.
SMRiP24626. Positions 19-317.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 5941Chitin-binding type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3979.
HOGENOMiHOG000231411.
InParanoidiP24626.
OMAiQNAAVAW.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24626-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRALALAVVA MAVVAVRGEQ CGSQAGGALC PNCLCCSQYG WCGSTSDYCG
60 70 80 90 100
AGCQSQCSGG CGGGPTPPSS GGGSGVASII SPSLFDQMLL HRNDQACAAK
110 120 130 140 150
GFYTYDAFVA AANAYPDFAT TGDADTCKRE VAAFLAQTSH ETTGGWPTAP
160 170 180 190 200
DGPYSWGYCF KEENNGNAPT YCEPKPEWPC AAGKKYYGRG PIQITYNYNY
210 220 230 240 250
GPAGQAIGSD LLNNPDLVAS DATVSFKTAF WFWMTPQSPK PSCHAVITGQ
260 270 280 290 300
WTPSADDQAA GRVPGYGEIT NIINGGVECG HGADDKVADR IGFYKRYCDM
310 320
LGVSYGDNLD CYNQRPYPPS
Length:320
Mass (Da):33,681
Last modified:November 22, 2005 - v2
Checksum:iD7331BF4A6B592E3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti98 – 981A → R in CAA38249 (PubMed:1893114).Curated
Sequence conflicti122 – 1221G → R in CAA38249 (PubMed:1893114).Curated
Sequence conflicti183 – 1831G → A in CAA38249 (PubMed:1893114).Curated
Sequence conflicti202 – 2065PAGQA → RGAG in CAA38249 (PubMed:1893114).Curated
Sequence conflicti223 – 2231Missing in CAA38249 (PubMed:1893114).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54367 Genomic DNA. Translation: CAA38249.1.
D16223 Genomic DNA. Translation: BAA03751.1.
AP003685 Genomic DNA. Translation: BAD61708.1.
AP004685 Genomic DNA. Translation: BAD61800.1.
AK061280 mRNA. No translation available.
PIRiS14948.
RefSeqiNP_001058626.1. NM_001065161.1.
UniGeneiOs.2692.

Genome annotation databases

EnsemblPlantsiOS06T0726100-01; OS06T0726100-01; OS06G0726100.
GeneIDi4342114.
KEGGiosa:4342114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54367 Genomic DNA. Translation: CAA38249.1.
D16223 Genomic DNA. Translation: BAA03751.1.
AP003685 Genomic DNA. Translation: BAD61708.1.
AP004685 Genomic DNA. Translation: BAD61800.1.
AK061280 mRNA. No translation available.
PIRiS14948.
RefSeqiNP_001058626.1. NM_001065161.1.
UniGeneiOs.2692.

3D structure databases

ProteinModelPortaliP24626.
SMRiP24626. Positions 19-317.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os06g51050.1.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS06T0726100-01; OS06T0726100-01; OS06G0726100.
GeneIDi4342114.
KEGGiosa:4342114.

Organism-specific databases

GrameneiP24626.

Phylogenomic databases

eggNOGiCOG3979.
HOGENOMiHOG000231411.
InParanoidiP24626.
OMAiQNAAVAW.

Enzyme and pathway databases

BRENDAi3.2.1.14. 4460.

Gene expression databases

ExpressionAtlasiP24626. baseline and differential.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a rice genomic clone that encodes a class I endochitinase."
    Huang J.K., Wen L., Swegle M., Tran H.C., Thin T.H., Naylor H.M., Muthukrishnan S., Reeck G.R.
    Plant Mol. Biol. 16:479-480(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Seedling.
  2. "Sequence variation, differential expression and chromosomal location of rice chitinase genes."
    Nishizawa Y., Kishimoto N., Saito A., Hibi T.
    Mol. Gen. Genet. 241:1-10(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  5. "Structure, heterologous expression, and properties of rice (Oryza sativa L.) family 19 chitinases."
    Truong N.-H., Park S.-M., Nishizawa Y., Watanabe T., Sasaki T., Itoh Y.
    Biosci. Biotechnol. Biochem. 67:1063-1070(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)."
    Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T.
    Genome 49:619-630(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.
  7. "Role of the loop structure of the catalytic domain in rice class I chitinase."
    Mizuno R., Fukamizo T., Sugiyama S., Nishizawa Y., Kezuka Y., Nonaka T., Suzuki K., Watanabe T.
    J. Biochem. 143:487-495(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCHI3_ORYSJ
AccessioniPrimary (citable) accession number: P24626
Secondary accession number(s): Q42994
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 22, 2005
Last modified: July 22, 2015
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.