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P24626 (CHI3_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chitinase 3

EC=3.2.1.14
Alternative name(s):
Basic endochitinase 1
Class I chitinase c
Short name=OsChia1c
Pathogenesis related (PR)-3 chitinase 3
Gene names
Name:Cht3
Synonyms:CH6
Ordered Locus Names:Os06g0726100, LOC_Os06g51050
ORF Names:P0017G10.2, P0548E04.22
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes chitin and plays a role in defense against fungal pathogens containing chitin. Inhibits the growth of T.reesei fungus on plate assay. Ref.5 Ref.7

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Tissue specificity

Expressed at low levels in roots, leaves, sheaths and meristems. Ref.6

Sequence similarities

Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.

Contains 1 chitin-binding type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 320302Chitinase 3
PRO_0000005305

Regions

Domain19 – 5941Chitin-binding type-1

Amino acid modifications

Disulfide bond21 ↔ 36 By similarity
Disulfide bond30 ↔ 42 By similarity
Disulfide bond33 ↔ 61 By similarity
Disulfide bond35 ↔ 49 By similarity
Disulfide bond53 ↔ 57 By similarity
Disulfide bond97 ↔ 159 By similarity
Disulfide bond172 ↔ 180 By similarity
Disulfide bond279 ↔ 311 By similarity

Experimental info

Sequence conflict981A → R in CAA38249. Ref.1
Sequence conflict1221G → R in CAA38249. Ref.1
Sequence conflict1831G → A in CAA38249. Ref.1
Sequence conflict202 – 2065PAGQA → RGAG in CAA38249. Ref.1
Sequence conflict2231Missing in CAA38249. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24626 [UniParc].

Last modified November 22, 2005. Version 2.
Checksum: D7331BF4A6B592E3

FASTA32033,681
        10         20         30         40         50         60 
MRALALAVVA MAVVAVRGEQ CGSQAGGALC PNCLCCSQYG WCGSTSDYCG AGCQSQCSGG 

        70         80         90        100        110        120 
CGGGPTPPSS GGGSGVASII SPSLFDQMLL HRNDQACAAK GFYTYDAFVA AANAYPDFAT 

       130        140        150        160        170        180 
TGDADTCKRE VAAFLAQTSH ETTGGWPTAP DGPYSWGYCF KEENNGNAPT YCEPKPEWPC 

       190        200        210        220        230        240 
AAGKKYYGRG PIQITYNYNY GPAGQAIGSD LLNNPDLVAS DATVSFKTAF WFWMTPQSPK 

       250        260        270        280        290        300 
PSCHAVITGQ WTPSADDQAA GRVPGYGEIT NIINGGVECG HGADDKVADR IGFYKRYCDM 

       310        320 
LGVSYGDNLD CYNQRPYPPS 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of a rice genomic clone that encodes a class I endochitinase."
Huang J.K., Wen L., Swegle M., Tran H.C., Thin T.H., Naylor H.M., Muthukrishnan S., Reeck G.R.
Plant Mol. Biol. 16:479-480(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Seedling.
[2]"Sequence variation, differential expression and chromosomal location of rice chitinase genes."
Nishizawa Y., Kishimoto N., Saito A., Hibi T.
Mol. Gen. Genet. 241:1-10(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[5]"Structure, heterologous expression, and properties of rice (Oryza sativa L.) family 19 chitinases."
Truong N.-H., Park S.-M., Nishizawa Y., Watanabe T., Sasaki T., Itoh Y.
Biosci. Biotechnol. Biochem. 67:1063-1070(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)."
Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T.
Genome 49:619-630(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.
[7]"Role of the loop structure of the catalytic domain in rice class I chitinase."
Mizuno R., Fukamizo T., Sugiyama S., Nishizawa Y., Kezuka Y., Nonaka T., Suzuki K., Watanabe T.
J. Biochem. 143:487-495(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54367 Genomic DNA. Translation: CAA38249.1.
D16223 Genomic DNA. Translation: BAA03751.1.
AP003685 Genomic DNA. Translation: BAD61708.1.
AP004685 Genomic DNA. Translation: BAD61800.1.
AK061280 mRNA. No translation available.
PIRS14948.
RefSeqNP_001058626.1. NM_001065161.1.
UniGeneOs.2692.

3D structure databases

ProteinModelPortalP24626.
SMRP24626. Positions 19-317.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS06T0726100-01; OS06T0726100-01; OS06G0726100.
GeneID4342114.
KEGGosa:4342114.

Organism-specific databases

GrameneP24626.

Phylogenomic databases

eggNOGCOG3979.
HOGENOMHOG000231411.
OMARNDQACA.
ProtClustDBCLSN2846287.

Family and domain databases

Gene3D3.30.60.10. 1 hit.
InterProIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFPIRSF001060. Endochitinase. 1 hit.
PRINTSPR00451. CHITINBINDNG.
ProDomPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHI3_ORYSJ
AccessionPrimary (citable) accession number: P24626
Secondary accession number(s): Q42994
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 22, 2005
Last modified: April 16, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries