P24626 (CHI3_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitinase 3
EC=3.2.1.14

Alternative name(s):
Basic endochitinase 1
Class I chitinase c
Short name=OsChia1c
Pathogenesis related (PR)-3 chitinase 3

Gene names

Name:	Cht3
Synonyms:	CH6
Ordered Locus Names:	Os06g0726100, LOC_Os06g51050
ORF Names:	P0017G10.2, P0548E04.22

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes chitin and plays a role in defense against fungal pathogens containing chitin. Inhibits the growth of T.reesei fungus on plate assay. Ref.5 Ref.7
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Tissue specificity	Expressed at low levels in roots, leaves, sheaths and meristems. Ref.6
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	amino sugar metabolic process Inferred from sequence or structural similarity Ref.1. Source: Gramene cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response to fungus Inferred from direct assay Ref.7. Source: UniProtKB jasmonic acid and ethylene-dependent systemic resistance, ethylene mediated signaling pathway Inferred from electronic annotation. Source: EnsemblPlants/Gramene polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene vacuole Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	beta-N-acetylglucosaminidase activity Inferred from sequence or structural similarity Ref.1. Source: Gramene chitin binding Inferred from sequence or structural similarity Ref.1. Source: Gramene endochitinase activity Inferred from sequence or structural similarity Ref.1. Source: Gramene
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 320	302	Chitinase 3		PRO_0000005305
Regions
	Domain	19 – 59	41	Chitin-binding type-1
Amino acid modifications
	Disulfide bond	21 ↔ 36		By similarity
	Disulfide bond	30 ↔ 42		By similarity
	Disulfide bond	33 ↔ 61		By similarity
	Disulfide bond	35 ↔ 49		By similarity
	Disulfide bond	53 ↔ 57		By similarity
	Disulfide bond	97 ↔ 159		By similarity
	Disulfide bond	172 ↔ 180		By similarity
	Disulfide bond	279 ↔ 311		By similarity
Experimental info
	Sequence conflict	98	1	A → R in CAA38249. Ref.1
	Sequence conflict	122	1	G → R in CAA38249. Ref.1
	Sequence conflict	183	1	G → A in CAA38249. Ref.1
	Sequence conflict	202 – 206	5	PAGQA → RGAG in CAA38249. Ref.1
	Sequence conflict	223	1	Missing in CAA38249. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24626 [UniParc]. Last modified November 22, 2005. Version 2. Checksum: D7331BF4A6B592E3	FASTA	320	33,681
	10 20 30 40 50 60 MRALALAVVA MAVVAVRGEQ CGSQAGGALC PNCLCCSQYG WCGSTSDYCG AGCQSQCSGG 70 80 90 100 110 120 CGGGPTPPSS GGGSGVASII SPSLFDQMLL HRNDQACAAK GFYTYDAFVA AANAYPDFAT 130 140 150 160 170 180 TGDADTCKRE VAAFLAQTSH ETTGGWPTAP DGPYSWGYCF KEENNGNAPT YCEPKPEWPC 190 200 210 220 230 240 AAGKKYYGRG PIQITYNYNY GPAGQAIGSD LLNNPDLVAS DATVSFKTAF WFWMTPQSPK 250 260 270 280 290 300 PSCHAVITGQ WTPSADDQAA GRVPGYGEIT NIINGGVECG HGADDKVADR IGFYKRYCDM 310 320 LGVSYGDNLD CYNQRPYPPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a rice genomic clone that encodes a class I endochitinase." Huang J.K., Wen L., Swegle M., Tran H.C., Thin T.H., Naylor H.M., Muthukrishnan S., Reeck G.R. Plant Mol. Biol. 16:479-480(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Seedling.
[2]	"Sequence variation, differential expression and chromosomal location of rice chitinase genes." Nishizawa Y., Kishimoto N., Saito A., Hibi T. Mol. Gen. Genet. 241:1-10(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"Structure, heterologous expression, and properties of rice (Oryza sativa L.) family 19 chitinases." Truong N.-H., Park S.-M., Nishizawa Y., Watanabe T., Sasaki T., Itoh Y. Biosci. Biotechnol. Biochem. 67:1063-1070(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)." Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T. Genome 49:619-630(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.
[7]	"Role of the loop structure of the catalytic domain in rice class I chitinase." Mizuno R., Fukamizo T., Sugiyama S., Nishizawa Y., Kezuka Y., Nonaka T., Suzuki K., Watanabe T. J. Biochem. 143:487-495(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54367 Genomic DNA. Translation: CAA38249.1. D16223 Genomic DNA. Translation: BAA03751.1. AP003685 Genomic DNA. Translation: BAD61708.1. AP004685 Genomic DNA. Translation: BAD61800.1. AK061280 mRNA. No translation available.
PIR	S14948.
RefSeq	NP_001058626.1. NM_001065161.1.
UniGene	Os.2692.
3D structure databases
ProteinModelPortal	P24626.
SMR	P24626. Positions 19-317.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os06g51050.1; LOC_Os06g51050.1; LOC_Os06g51050.
GeneID	4342114.
KEGG	dosa:Os06t0726100-01. osa:4342114.
Organism-specific databases
Gramene	P24626.
Phylogenomic databases
eggNOG	COG3979.
HOGENOM	HOG000231411.
OMA	SAPEAYC.
ProtClustDB	CLSN2846287.
Gene expression databases
ArrayExpress	P24626.
Family and domain databases
Gene3D	3.30.60.10. 1 hit.
InterPro	IPR001002. Chitin-bd_1. IPR018371. Chitin-binding_1_CS. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. IPR023346. Lysozyme-like_dom. [Graphical view]
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_bd_1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
SUPFAM	SSF57016. Chitin_bd_1. 1 hit. SSF53955. SSF53955. 1 hit.
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI3_ORYSJ

Accession

Primary (citable) accession number: P24626
Secondary accession number(s): Q42994

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	November 22, 2005
Last modified:	April 3, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program