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P24626

- CHI3_ORYSJ

UniProt

P24626 - CHI3_ORYSJ

Protein

Chitinase 3

Gene

Cht3

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (22 Nov 2005)
      Previous versions | rss
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    Functioni

    Hydrolyzes chitin and plays a role in defense against fungal pathogens containing chitin. Inhibits the growth of T.reesei fungus on plate assay.2 Publications

    Catalytic activityi

    Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

    GO - Molecular functioni

    1. beta-N-acetylglucosaminidase activity Source: Gramene
    2. chitinase activity Source: UniProtKB
    3. chitin binding Source: Gramene
    4. endochitinase activity Source: Gramene

    GO - Biological processi

    1. amino sugar metabolic process Source: Gramene
    2. cell wall macromolecule catabolic process Source: InterPro
    3. chitin catabolic process Source: UniProtKB-KW
    4. defense response to fungus Source: UniProtKB
    5. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

    Keywords - Ligandi

    Chitin-binding

    Protein family/group databases

    CAZyiCBM18. Carbohydrate-Binding Module Family 18.
    GH19. Glycoside Hydrolase Family 19.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chitinase 3 (EC:3.2.1.14)
    Alternative name(s):
    Basic endochitinase 1
    Class I chitinase c
    Short name:
    OsChia1c
    Pathogenesis related (PR)-3 chitinase 3
    Gene namesi
    Name:Cht3
    Synonyms:CH6
    Ordered Locus Names:Os06g0726100, LOC_Os06g51050
    ORF Names:P0017G10.2, P0548E04.22
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 6

    Organism-specific databases

    GrameneiP24626.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 320302Chitinase 3PRO_0000005305Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi21 ↔ 36PROSITE-ProRule annotation
    Disulfide bondi30 ↔ 42PROSITE-ProRule annotation
    Disulfide bondi33 ↔ 61PROSITE-ProRule annotation
    Disulfide bondi35 ↔ 49PROSITE-ProRule annotation
    Disulfide bondi53 ↔ 57PROSITE-ProRule annotation
    Disulfide bondi97 ↔ 159PROSITE-ProRule annotation
    Disulfide bondi172 ↔ 180PROSITE-ProRule annotation
    Disulfide bondi279 ↔ 311PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed at low levels in roots, leaves, sheaths and meristems.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP24626.
    SMRiP24626. Positions 19-317.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 5941Chitin-binding type-1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3979.
    HOGENOMiHOG000231411.
    OMAiHNAIVNG.

    Family and domain databases

    Gene3Di3.30.60.10. 1 hit.
    InterProiIPR001002. Chitin-bd_1.
    IPR018371. Chitin-binding_1_CS.
    IPR016283. Glyco_hydro_19.
    IPR000726. Glyco_hydro_19_cat.
    IPR023346. Lysozyme-like_dom.
    [Graphical view]
    PfamiPF00187. Chitin_bind_1. 1 hit.
    PF00182. Glyco_hydro_19. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001060. Endochitinase. 1 hit.
    PRINTSiPR00451. CHITINBINDNG.
    ProDomiPD000609. Chitin_bd_1. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00270. ChtBD1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53955. SSF53955. 1 hit.
    SSF57016. SSF57016. 1 hit.
    PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
    PS50941. CHIT_BIND_I_2. 1 hit.
    PS00773. CHITINASE_19_1. 1 hit.
    PS00774. CHITINASE_19_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24626-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRALALAVVA MAVVAVRGEQ CGSQAGGALC PNCLCCSQYG WCGSTSDYCG    50
    AGCQSQCSGG CGGGPTPPSS GGGSGVASII SPSLFDQMLL HRNDQACAAK 100
    GFYTYDAFVA AANAYPDFAT TGDADTCKRE VAAFLAQTSH ETTGGWPTAP 150
    DGPYSWGYCF KEENNGNAPT YCEPKPEWPC AAGKKYYGRG PIQITYNYNY 200
    GPAGQAIGSD LLNNPDLVAS DATVSFKTAF WFWMTPQSPK PSCHAVITGQ 250
    WTPSADDQAA GRVPGYGEIT NIINGGVECG HGADDKVADR IGFYKRYCDM 300
    LGVSYGDNLD CYNQRPYPPS 320
    Length:320
    Mass (Da):33,681
    Last modified:November 22, 2005 - v2
    Checksum:iD7331BF4A6B592E3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti98 – 981A → R in CAA38249. (PubMed:1893114)Curated
    Sequence conflicti122 – 1221G → R in CAA38249. (PubMed:1893114)Curated
    Sequence conflicti183 – 1831G → A in CAA38249. (PubMed:1893114)Curated
    Sequence conflicti202 – 2065PAGQA → RGAG in CAA38249. (PubMed:1893114)Curated
    Sequence conflicti223 – 2231Missing in CAA38249. (PubMed:1893114)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54367 Genomic DNA. Translation: CAA38249.1.
    D16223 Genomic DNA. Translation: BAA03751.1.
    AP003685 Genomic DNA. Translation: BAD61708.1.
    AP004685 Genomic DNA. Translation: BAD61800.1.
    AK061280 mRNA. No translation available.
    PIRiS14948.
    RefSeqiNP_001058626.1. NM_001065161.1.
    UniGeneiOs.2692.

    Genome annotation databases

    EnsemblPlantsiOS06T0726100-01; OS06T0726100-01; OS06G0726100.
    GeneIDi4342114.
    KEGGiosa:4342114.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54367 Genomic DNA. Translation: CAA38249.1 .
    D16223 Genomic DNA. Translation: BAA03751.1 .
    AP003685 Genomic DNA. Translation: BAD61708.1 .
    AP004685 Genomic DNA. Translation: BAD61800.1 .
    AK061280 mRNA. No translation available.
    PIRi S14948.
    RefSeqi NP_001058626.1. NM_001065161.1.
    UniGenei Os.2692.

    3D structure databases

    ProteinModelPortali P24626.
    SMRi P24626. Positions 19-317.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM18. Carbohydrate-Binding Module Family 18.
    GH19. Glycoside Hydrolase Family 19.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS06T0726100-01 ; OS06T0726100-01 ; OS06G0726100 .
    GeneIDi 4342114.
    KEGGi osa:4342114.

    Organism-specific databases

    Gramenei P24626.

    Phylogenomic databases

    eggNOGi COG3979.
    HOGENOMi HOG000231411.
    OMAi HNAIVNG.

    Family and domain databases

    Gene3Di 3.30.60.10. 1 hit.
    InterProi IPR001002. Chitin-bd_1.
    IPR018371. Chitin-binding_1_CS.
    IPR016283. Glyco_hydro_19.
    IPR000726. Glyco_hydro_19_cat.
    IPR023346. Lysozyme-like_dom.
    [Graphical view ]
    Pfami PF00187. Chitin_bind_1. 1 hit.
    PF00182. Glyco_hydro_19. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001060. Endochitinase. 1 hit.
    PRINTSi PR00451. CHITINBINDNG.
    ProDomi PD000609. Chitin_bd_1. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00270. ChtBD1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53955. SSF53955. 1 hit.
    SSF57016. SSF57016. 1 hit.
    PROSITEi PS00026. CHIT_BIND_I_1. 1 hit.
    PS50941. CHIT_BIND_I_2. 1 hit.
    PS00773. CHITINASE_19_1. 1 hit.
    PS00774. CHITINASE_19_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of a rice genomic clone that encodes a class I endochitinase."
      Huang J.K., Wen L., Swegle M., Tran H.C., Thin T.H., Naylor H.M., Muthukrishnan S., Reeck G.R.
      Plant Mol. Biol. 16:479-480(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Seedling.
    2. "Sequence variation, differential expression and chromosomal location of rice chitinase genes."
      Nishizawa Y., Kishimoto N., Saito A., Hibi T.
      Mol. Gen. Genet. 241:1-10(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.
    5. "Structure, heterologous expression, and properties of rice (Oryza sativa L.) family 19 chitinases."
      Truong N.-H., Park S.-M., Nishizawa Y., Watanabe T., Sasaki T., Itoh Y.
      Biosci. Biotechnol. Biochem. 67:1063-1070(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)."
      Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T.
      Genome 49:619-630(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.
    7. "Role of the loop structure of the catalytic domain in rice class I chitinase."
      Mizuno R., Fukamizo T., Sugiyama S., Nishizawa Y., Kezuka Y., Nonaka T., Suzuki K., Watanabe T.
      J. Biochem. 143:487-495(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCHI3_ORYSJ
    AccessioniPrimary (citable) accession number: P24626
    Secondary accession number(s): Q42994
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: November 22, 2005
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3