Basic endochitinase 1 precursor - Oryza sativa subsp. japonica (Rice)

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Reviewed, UniProtKB/Swiss-Prot P24626 (CHI1_ORYSJ)

Last modified June 16, 2009. Version 78. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Basic endochitinase 1
EC=3.2.1.14

Gene names

Name:	Cht-3
Ordered Locus Names:	Os06g0726100, LOC_Os06g51050
ORF Names:	P0017G10.2, P0548E04.22

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

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Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function

Defense against chitin containing fungal pathogens.

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sequence similarities

Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.

Contains 1 chitin-binding type-1 domain.

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Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

Potential

Chain

19 – 320

302

Basic endochitinase 1

PRO_0000005305

Regions

Domain

19 – 59

Chitin-binding type-1

Amino acid modifications

Disulfide bond

21 ↔ 36

By similarity

Disulfide bond

30 ↔ 42

By similarity

Disulfide bond

35 ↔ 49

By similarity

Disulfide bond

53 ↔ 57

By similarity

Disulfide bond

97 ↔ 159

By similarity

Disulfide bond

172 ↔ 180

By similarity

Disulfide bond

279 ↔ 311

By similarity

Experimental info

Sequence conflict

A → R in CAA38249. Ref.1

Sequence conflict

122

G → R in CAA38249. Ref.1

Sequence conflict

183

G → A in CAA38249. Ref.1

Sequence conflict

202 – 206

PAGQA → RGAG in CAA38249. Ref.1

Sequence conflict

223

Missing in CAA38249. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P24626-1 [UniParc].

Last modified November 22, 2005. Version 2.
Checksum: D7331BF4A6B592E3
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FASTA

320

33,681

        10         20         30         40         50         60 
MRALALAVVA MAVVAVRGEQ CGSQAGGALC PNCLCCSQYG WCGSTSDYCG AGCQSQCSGG 

        70         80         90        100        110        120 
CGGGPTPPSS GGGSGVASII SPSLFDQMLL HRNDQACAAK GFYTYDAFVA AANAYPDFAT 

       130        140        150        160        170        180 
TGDADTCKRE VAAFLAQTSH ETTGGWPTAP DGPYSWGYCF KEENNGNAPT YCEPKPEWPC 

       190        200        210        220        230        240 
AAGKKYYGRG PIQITYNYNY GPAGQAIGSD LLNNPDLVAS DATVSFKTAF WFWMTPQSPK 

       250        260        270        280        290        300 
PSCHAVITGQ WTPSADDQAA GRVPGYGEIT NIINGGVECG HGADDKVADR IGFYKRYCDM 

       310        320 
LGVSYGDNLD CYNQRPYPPS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a rice genomic clone that encodes a class I endochitinase." Huang J.K., Wen L., Swegle M., Tran H.C., Thin T.H., Naylor H.M., Muthukrishnan S., Reeck G.R. Plant Mol. Biol. 16:479-480(1991) [PubMed: 1893114] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Seedling.
[2]	"Sequence variation, differential expression and chromosomal location of rice chitinase genes." Nishizawa Y., Kishimoto N., Saito A., Hibi T. Mol. Gen. Genet. 241:1-10(1993) [PubMed: 7901749] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Oryza sativa nipponbare chromosome 6 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

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Cross-references

Sequence databases
	X54367 Genomic DNA. Translation: CAA38249.1. D16223 Genomic DNA. Translation: BAA03751.1. AP003685 Genomic DNA. Translation: BAD61708.1. AP004685 Genomic DNA. Translation: BAD61800.1. AK061280 mRNA. No translation available.
PIR	S14948.
RefSeq	NP_001058626.1.
UniGene	Os.2692
3D structure databases
HSSP	HSSP built from PDB template 1HEV based on UniProtKB P02877.
SMR	P24626. Positions 19-317.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Genome annotation databases
GeneID	4342114.
KEGG	osa:4342114.
Organism-specific databases
Gramene	P24626.
Family and domain databases
InterPro	IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_binding_1. 1 hit. PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

CHI1_ORYSJ

Accession

Primary (citable) accession number: P24626
Secondary accession number(s): Q42994

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	November 22, 2005
Last modified:	June 16, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Family and domain databases

Entry information

Relevant documents