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Protein

Alpha-crystallin A chain

Gene

Cryaa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Contributes to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi102 – 1021Zinc 1By similarity
Metal bindingi123 – 1231Zinc 2By similarity
Metal bindingi125 – 1251Zinc 2By similarity
Metal bindingi130 – 1301Zinc 1By similarity
Metal bindingi138 – 1381Zinc 1By similarity

GO - Molecular functioni

GO - Biological processi

  • actin filament organization Source: MGI
  • apoptotic process Source: MGI
  • apoptotic process involved in morphogenesis Source: MGI
  • camera-type eye development Source: MGI
  • embryonic camera-type eye morphogenesis Source: MGI
  • lens development in camera-type eye Source: MGI
  • lens fiber cell morphogenesis Source: MGI
  • lens morphogenesis in camera-type eye Source: MGI
  • microtubule-based process Source: MGI
  • mitochondrion organization Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • negative regulation of gene expression Source: MGI
  • positive regulation of cell growth Source: MGI
  • positive regulation of protein phosphorylation Source: MGI
  • protein folding Source: Ensembl
  • protein homooligomerization Source: Ensembl
  • response to drug Source: Ensembl
  • response to glucocorticoid Source: Ensembl
  • response to hydrogen peroxide Source: MGI
  • response to hypoxia Source: MGI
  • response to lead ion Source: Ensembl
  • response to UV-A Source: MGI
  • tubulin complex assembly Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Eye lens protein

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-crystallin A chain
Gene namesi
Name:Cryaa
Synonyms:Crya1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:88515. Cryaa.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196Alpha-crystallin A chainPRO_0000125872Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCurated
Modified residuei6 – 61Deamidated glutamine; partialBy similarity
Modified residuei21 – 211Omega-N-methylated arginineBy similarity
Modified residuei45 – 451PhosphoserineBy similarity
Modified residuei50 – 501Deamidated glutamine; partialBy similarity
Modified residuei93 – 931N6-acetyllysineBy similarity
Modified residuei122 – 1221N6-acetyllysineBy similarity
Modified residuei124 – 1241Deamidated asparagine; partialBy similarity
Modified residuei145 – 1451PhosphoserineBy similarity
Modified residuei146 – 1461Deamidated asparagine; partialBy similarity
Modified residuei170 – 1701Deamidated glutamine; partialBy similarity
Glycosylationi185 – 1851O-linked (GlcNAc)By similarity

Post-translational modificationi

Acetylation at Lys-93 seems to increase chaperone activity.By similarity
Undergoes age-dependent proteolytical cleavage at the C-terminus.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

PaxDbiP24622.
PRIDEiP24622.

PTM databases

iPTMnetiP24622.
PhosphoSiteiP24622.
UniCarbKBiP24622.

Expressioni

Gene expression databases

BgeeiP24622.
CleanExiMM_CRYAA.
ExpressionAtlasiP24622. baseline and differential.
GenevisibleiP24622. MM.

Interactioni

Subunit structurei

Heteropolymer composed of three CRYAA and one CRYAB subunits. Inter-subunit bridging via zinc ions enhances stability, which is crucial as there is no protein turn over in the lens. Can also form homodimers and higher homooligomers (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000019192.

Structurei

3D structure databases

ProteinModelPortaliP24622.
SMRiP24622. Positions 1-191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small heat shock protein (HSP20) family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3591. Eukaryota.
ENOG410YERS. LUCA.
HOGENOMiHOG000233954.
HOVERGENiHBG054766.
InParanoidiP24622.
KOiK09541.
OMAiGPKVQSG.
OrthoDBiEOG7WHHBK.
PhylomeDBiP24622.
TreeFamiTF105049.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiIPR002068. A-crystallin/Hsp20_dom.
IPR001436. Alpha-crystallin/HSP.
IPR003090. Alpha-crystallin_N.
IPR008978. HSP20-like_chaperone.
[Graphical view]
PfamiPF00525. Crystallin. 1 hit.
PF00011. HSP20. 1 hit.
[Graphical view]
PRINTSiPR00299. ACRYSTALLIN.
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS01031. HSP20. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P24622-1) [UniParc]FASTAAdd to basket

Also known as: Minor, Alpha-A(ins)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDVTIQHPWF KRALGPFYPS RLFDQFFGEG LFEYDLLPFL SSTISPYYRQ
60 70 80 90 100
SLFRTVLDSG ISELMTHMWF VMHQPHAGNP KNNPVKVRSD RDKFVIFLDV
110 120 130 140 150
KHFSPEDLTV KVLEDFVEIH GKHNERQDDH GYISREFHRR YRLPSNVDQS
160 170 180 190
ALSCSLSADG MLTFSGPKVQ SGLDAGHSER AIPVSREEKP SSAPSS
Note: Constitutes approximately 10% of the total alpha-A-crystallin in the lens.
Length:196
Mass (Da):22,489
Last modified:March 1, 1992 - v1
Checksum:iA6063BB21EEB8E78
GO
Isoform 2 (identifier: P24622-2) [UniParc]FASTAAdd to basket

Also known as: Major

The sequence of this isoform differs from the canonical sequence as follows:
     64-86: Missing.

Show »
Length:173
Mass (Da):19,792
Checksum:iE146E3F488591F93
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei64 – 8623Missing in isoform 2. 1 PublicationVSP_011916Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00730 Genomic DNA. Translation: CAA24108.2.
V00730 Genomic DNA. Translation: CAA24109.2.
J00375 Genomic DNA. Translation: AAA37469.1.
J00375 Genomic DNA. Translation: AAA37470.1.
J00376 mRNA. Translation: AAA37471.1.
CCDSiCCDS37551.1. [P24622-1]
PIRiA02893. CYMSA.
B18860.
RefSeqiNP_038529.1. NM_013501.3. [P24622-1]
XP_006523637.1. XM_006523574.2. [P24622-1]
UniGeneiMm.1228.

Genome annotation databases

EnsembliENSMUST00000019192; ENSMUSP00000019192; ENSMUSG00000024041. [P24622-1]
GeneIDi12954.
KEGGimmu:12954.
UCSCiuc008bvp.2. mouse. [P24622-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00730 Genomic DNA. Translation: CAA24108.2.
V00730 Genomic DNA. Translation: CAA24109.2.
J00375 Genomic DNA. Translation: AAA37469.1.
J00375 Genomic DNA. Translation: AAA37470.1.
J00376 mRNA. Translation: AAA37471.1.
CCDSiCCDS37551.1. [P24622-1]
PIRiA02893. CYMSA.
B18860.
RefSeqiNP_038529.1. NM_013501.3. [P24622-1]
XP_006523637.1. XM_006523574.2. [P24622-1]
UniGeneiMm.1228.

3D structure databases

ProteinModelPortaliP24622.
SMRiP24622. Positions 1-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000019192.

PTM databases

iPTMnetiP24622.
PhosphoSiteiP24622.
UniCarbKBiP24622.

Proteomic databases

PaxDbiP24622.
PRIDEiP24622.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019192; ENSMUSP00000019192; ENSMUSG00000024041. [P24622-1]
GeneIDi12954.
KEGGimmu:12954.
UCSCiuc008bvp.2. mouse. [P24622-1]

Organism-specific databases

CTDi1409.
MGIiMGI:88515. Cryaa.

Phylogenomic databases

eggNOGiKOG3591. Eukaryota.
ENOG410YERS. LUCA.
HOGENOMiHOG000233954.
HOVERGENiHBG054766.
InParanoidiP24622.
KOiK09541.
OMAiGPKVQSG.
OrthoDBiEOG7WHHBK.
PhylomeDBiP24622.
TreeFamiTF105049.

Miscellaneous databases

ChiTaRSiCryaa. mouse.
PROiP24622.
SOURCEiSearch...

Gene expression databases

BgeeiP24622.
CleanExiMM_CRYAA.
ExpressionAtlasiP24622. baseline and differential.
GenevisibleiP24622. MM.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiIPR002068. A-crystallin/Hsp20_dom.
IPR001436. Alpha-crystallin/HSP.
IPR003090. Alpha-crystallin_N.
IPR008978. HSP20-like_chaperone.
[Graphical view]
PfamiPF00525. Crystallin. 1 hit.
PF00011. HSP20. 1 hit.
[Graphical view]
PRINTSiPR00299. ACRYSTALLIN.
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS01031. HSP20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Alternative RNA splicing of the murine alpha A-crystallin gene: protein-coding information within an intron."
    King C.R., Piatigorsky J.
    Cell 32:707-712(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
  2. "Alpha A-crystallin messenger RNA of the mouse lens: more noncoding than coding sequences."
    King C.R., Shinohara T., Piatigorsky J.
    Science 215:985-987(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-196 (ISOFORM 2).

Entry informationi

Entry nameiCRYAA_MOUSE
AccessioniPrimary (citable) accession number: P24622
Secondary accession number(s): P02490
, P02496, P82532, Q61444, Q6LEL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 1, 1992
Last modified: June 8, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.