P24617 (T2P2_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme NgoPII
Short name=R.NgoPII
EC=3.1.21.4

Alternative name(s):
Endonuclease NgoPII
Type II restriction enzyme NgoPII

Gene names

Name:	ngoPIIR
Synonyms:	dcrB

Organism

Neisseria gonorrhoeae

Taxonomic identifier

485 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGCC and cleaves after G-2.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Type-2 restriction enzyme NgoPII		PRO_0000077348

Sequences

	Sequence		Length	Mass (Da)
	P24617 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 1BF8B25BEEC96903	FASTA	278	31,501
	10 20 30 40 50 60 MNIIDAIINL ANNPVVGVES HSQSNNRANQ AGDALEEYVK DLFSGSFNLN ETQRIARHAK 70 80 90 100 110 120 VFSYLGNNSN PPDAMLRNGD AIEVKKIESK DSALALNSSH PKSKLSVDDS MLTKACKDAE 130 140 150 160 170 180 KWEEKDIIYI VGVVDKKKNL KHLAMVYGID YCADAECYLK IKNQIKEGIG NIGGIQFAET 190 200 210 220 230 240 KELGRVNRID PLNITYLRVR GMWGIENPWF VFNYIYQRNM EKSFNFMAII NEDKWNSFNN 250 260 270 TDKLLAIQDS KLAISDIKIK NPNNPARLRN AKLITYHL

References

[1]	"Nucleotide sequence and genetic organization of the NgoPII restriction-modification system of Neisseria gonorrhoeae." Sullivan K.M., Saunders J.R. Mol. Gen. Genet. 216:380-387(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: P9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52661 Genomic DNA. Translation: CAA36887.1.
PIR	S04419.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1331. NgoPII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019046. Restrct_endonuc_II_NgoPII. [Graphical view]
Pfam	PF09521. RE_NgoPII. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2P2_NEIGO

Accession

Primary (citable) accession number: P24617

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program