Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Degenerin mec-4

Gene

mec-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable sodium channel subunit (PubMed:12478294). May be needed for mechanosensory transduction (touch sensitivity) (PubMed:8655580). Negatively regulates the turning step of male mating behavior (PubMed:17611271).3 Publications

Miscellaneous

Mutations in mec-4 results in the degeneration of a small set of neurons which typically swell to several times their normal diameter before they disappear, presumably due to lysis.1 Publication

GO - Molecular functioni

  • ligand-gated sodium channel activity Source: WormBase

GO - Biological processi

  • detection of mechanical stimulus involved in sensory perception of touch Source: UniProtKB
  • mechanosensory behavior Source: UniProtKB
  • negative regulation of turning behavior involved in mating Source: WormBase
  • positive regulation of detection of mechanical stimulus involved in sensory perception of touch Source: UniProtKB
  • positive regulation of mechanosensory behavior Source: UniProtKB
  • response to mechanical stimulus Source: WormBase

Keywordsi

Molecular functionIon channel, Sodium channel
Biological processIon transport, Sodium transport, Transport
LigandSodium

Enzyme and pathway databases

ReactomeiR-CEL-2672351. Stimuli-sensing channels.

Protein family/group databases

TCDBi1.A.6.2.2. the epithelial na(+) channel (enac) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Degenerin mec-4
Alternative name(s):
Mechanosensory abnormality protein 4
Gene namesi
Name:mec-4
Synonyms:mec-13
ORF Names:T01C8.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiT01C8.7; CE39109; WBGene00003168; mec-4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 109CytoplasmicSequence analysisAdd BLAST109
Transmembranei110 – 130HelicalSequence analysisAdd BLAST21
Topological domaini131 – 718ExtracellularSequence analysisAdd BLAST588
Transmembranei719 – 739HelicalSequence analysisAdd BLAST21
Topological domaini740 – 768CytoplasmicSequence analysisAdd BLAST29

GO - Cellular componenti

  • axon Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: WormBase
  • neuron projection membrane Source: WormBase

Keywords - Cellular componenti

Cell projection, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi230G → E in e1339; abnormal repetitive turning behavior during male mating. 1 Publication1
Mutagenesisi713A → G, S or C: No degeneration. 1 Publication1
Mutagenesisi713A → R, D, F, L, P, T or V: Degeneration. 1 Publication1

Keywords - Diseasei

Neurodegeneration

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001812871 – 768Degenerin mec-4Add BLAST768

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi335N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi356N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi480N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi484N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi503N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi671N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP24612.

Expressioni

Tissue specificityi

Expressed in AVM, ALM, PVM and PLM touch neurons.2 Publications

Gene expression databases

BgeeiWBGene00003168.

Interactioni

Subunit structurei

The channel is probably composed of at least the mec-2, mec-4, mec-6 and mec-10 subunits.1 Publication

Protein-protein interaction databases

STRINGi6239.T01C8.7.2.

Structurei

Secondary structure

1768
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 8Combined sources7
Helixi17 – 22Combined sources6
Helixi27 – 36Combined sources10
Helixi41 – 45Combined sources5
Beta strandi48 – 50Combined sources3
Turni51 – 53Combined sources3
Turni63 – 66Combined sources4
Beta strandi70 – 74Combined sources5
Helixi78 – 87Combined sources10
Beta strandi98 – 100Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2K2BNMR-A1-103[»]
5TTTNMR-A1-103[»]
ProteinModelPortaliP24612.
SMRiP24612.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24612.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4294. Eukaryota.
ENOG410ZNFK. LUCA.
GeneTreeiENSGT00760000119120.
HOGENOMiHOG000018424.
InParanoidiP24612.
KOiK03440.
OMAiFCYKTSA.
OrthoDBiEOG091G0KW6.
PhylomeDBiP24612.

Family and domain databases

InterProiView protein in InterPro
IPR004726. Deg-1.
IPR001873. ENaC.
IPR020903. ENaC_CS.
PANTHERiPTHR11690. PTHR11690. 1 hit.
PfamiView protein in Pfam
PF00858. ASC. 1 hit.
PRINTSiPR01078. AMINACHANNEL.
TIGRFAMsiTIGR00867. deg-1. 1 hit.
PROSITEiView protein in PROSITE
PS01206. ASC. 1 hit.

Sequencei

Sequence statusi: Complete.

P24612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWMQNLKNY QHLRDPSEYM SQVYGDPLAY LQETTKFVTE REYYEDFGYG
60 70 80 90 100
ECFNSTESEV QCELITGEFD PKLLPYDKRL AWHFKEFCYK TSAHGIPMIG
110 120 130 140 150
EAPNVYYRAV WVVLFLGCMI MLYLNAQSVL DKYNRNEKIV DIQLKFDTAP
160 170 180 190 200
FPAITLCNLN PYKASLATSV DLVKRTLSAF DGAMGKAGGN KDHEEEREVV
210 220 230 240 250
TEPPTTPAPT TKPARRRGKR DLSGAFFEPG FARCLCGSQG SSEQEDKDEE
260 270 280 290 300
KEEELLETTT KKVFNINDAD EEWDGMEEYD NEHYENYDVE ATTGMNMMEE
310 320 330 340 350
CQSERTKFDE PTGFDDRCIC AFDRSTHDAW PCFLNGTWET TECDTCNEHA
360 370 380 390 400
FCTKDNKTAK GHRSPCICAP SRFCVAYNGK TPPIEIWTYL QGGTPTEDPN
410 420 430 440 450
FLEAMGFQGM TDEVAIVTKA KENIMFAMAT LSMQDRERLS TTKRELVHKC
460 470 480 490 500
SFNGKACDIE ADFLTHIDPA FGSCFTFNHN RTVNLTSIRA GPMYGLRMLV
510 520 530 540 550
YVNASDYMPT TEATGVRLTI HDKEDFPFPD TFGYSAPTGY VSSFGLRLRK
560 570 580 590 600
MSRLPAPYGD CVPDGKTSDY IYSNYEYSVE GCYRSCFQQL VLKECRCGDP
610 620 630 640 650
RFPVPENARH CDAADPIARK CLDARMNDLG GLHGSFRCRC QQPCRQSIYS
660 670 680 690 700
VTYSPAKWPS LSLQIQLGSC NGTAVECNKH YKENGAMVEV FYEQLNFEML
710 720 730 740 750
TESEAYGFVN LLADFGGQLG LWCGISFLTC CEFVFLFLET AYMSAEHNYS
760
LYKKKKAEKA KKIASGSF
Length:768
Mass (Da):87,194
Last modified:November 1, 1997 - v3
Checksum:i8899D0FCCB7F62C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53669 Genomic DNA. Translation: AAC47265.1.
FO081053 Genomic DNA. Translation: CCD68838.1.
X58982 Genomic DNA. Translation: CAA41731.1.
PIRiT29859.
RefSeqiNP_510712.2. NM_078311.5.
UniGeneiCel.634.

Genome annotation databases

EnsemblMetazoaiT01C8.7.1; T01C8.7.1; WBGene00003168.
T01C8.7.2; T01C8.7.2; WBGene00003168.
GeneIDi181728.
KEGGicel:CELE_T01C8.7.
UCSCiT01C8.7. c. elegans.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMEC4_CAEEL
AccessioniPrimary (citable) accession number: P24612
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: July 5, 2017
This is version 130 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families