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Protein

Degenerin mec-4

Gene

mec-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable sodium channel subunit. May be needed for mechanosensory transduction (touch sensitivity).

GO - Molecular functioni

  • ligand-gated sodium channel activity Source: WormBase

GO - Biological processi

  • mechanosensory behavior Source: WormBase
  • negative regulation of turning behavior involved in mating Source: WormBase
  • response to mechanical stimulus Source: WormBase
  • sodium ion transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Sodium channel

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-CEL-2672351. Stimuli-sensing channels.

Protein family/group databases

TCDBi1.A.6.2.2. the epithelial na(+) channel (enac) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Degenerin mec-4
Alternative name(s):
Mechanosensory abnormality protein 4
Gene namesi
Name:mec-4
Synonyms:mec-13
ORF Names:T01C8.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiT01C8.7; CE39109; WBGene00003168; mec-4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 109109CytoplasmicSequence analysisAdd
BLAST
Transmembranei110 – 13021HelicalSequence analysisAdd
BLAST
Topological domaini131 – 718588ExtracellularSequence analysisAdd
BLAST
Transmembranei719 – 73921HelicalSequence analysisAdd
BLAST
Topological domaini740 – 76829CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: WormBase
  • neuron projection membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi713 – 7131A → G, S or C: No degeneration. 1 Publication
Mutagenesisi713 – 7131A → R, D, F, L, P, T or V: Degeneration. 1 Publication

Keywords - Diseasei

Neurodegeneration

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 768768Degenerin mec-4PRO_0000181287Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi335 – 3351N-linked (GlcNAc...)Sequence analysis
Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence analysis
Glycosylationi480 – 4801N-linked (GlcNAc...)Sequence analysis
Glycosylationi484 – 4841N-linked (GlcNAc...)Sequence analysis
Glycosylationi503 – 5031N-linked (GlcNAc...)Sequence analysis
Glycosylationi671 – 6711N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP24612.

Expressioni

Tissue specificityi

Coexpressed with mec-6 in touch-cell axons.1 Publication

Interactioni

Subunit structurei

The channel is probably composed of at least the mec-2, mec-4, mec-6 and mec-10 subunits.

Protein-protein interaction databases

STRINGi6239.T01C8.7.2.

Structurei

Secondary structure

1
768
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 87Combined sources
Helixi17 – 226Combined sources
Helixi27 – 3610Combined sources
Helixi41 – 455Combined sources
Turni51 – 533Combined sources
Turni63 – 664Combined sources
Beta strandi70 – 745Combined sources
Helixi78 – 8710Combined sources
Beta strandi98 – 1003Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K2BNMR-A1-103[»]
ProteinModelPortaliP24612.
SMRiP24612. Positions 1-103, 444-733.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24612.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4294. Eukaryota.
ENOG410ZNFK. LUCA.
GeneTreeiENSGT00760000119120.
HOGENOMiHOG000018424.
InParanoidiP24612.
OMAiQCELITG.
PhylomeDBiP24612.

Family and domain databases

InterProiIPR004726. Deg-1.
IPR001873. Na+channel_ASC.
IPR020903. Na+channel_ASC_CS.
[Graphical view]
PANTHERiPTHR11690. PTHR11690. 3 hits.
PfamiPF00858. ASC. 1 hit.
[Graphical view]
PRINTSiPR01078. AMINACHANNEL.
TIGRFAMsiTIGR00867. deg-1. 1 hit.
PROSITEiPS01206. ASC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWMQNLKNY QHLRDPSEYM SQVYGDPLAY LQETTKFVTE REYYEDFGYG
60 70 80 90 100
ECFNSTESEV QCELITGEFD PKLLPYDKRL AWHFKEFCYK TSAHGIPMIG
110 120 130 140 150
EAPNVYYRAV WVVLFLGCMI MLYLNAQSVL DKYNRNEKIV DIQLKFDTAP
160 170 180 190 200
FPAITLCNLN PYKASLATSV DLVKRTLSAF DGAMGKAGGN KDHEEEREVV
210 220 230 240 250
TEPPTTPAPT TKPARRRGKR DLSGAFFEPG FARCLCGSQG SSEQEDKDEE
260 270 280 290 300
KEEELLETTT KKVFNINDAD EEWDGMEEYD NEHYENYDVE ATTGMNMMEE
310 320 330 340 350
CQSERTKFDE PTGFDDRCIC AFDRSTHDAW PCFLNGTWET TECDTCNEHA
360 370 380 390 400
FCTKDNKTAK GHRSPCICAP SRFCVAYNGK TPPIEIWTYL QGGTPTEDPN
410 420 430 440 450
FLEAMGFQGM TDEVAIVTKA KENIMFAMAT LSMQDRERLS TTKRELVHKC
460 470 480 490 500
SFNGKACDIE ADFLTHIDPA FGSCFTFNHN RTVNLTSIRA GPMYGLRMLV
510 520 530 540 550
YVNASDYMPT TEATGVRLTI HDKEDFPFPD TFGYSAPTGY VSSFGLRLRK
560 570 580 590 600
MSRLPAPYGD CVPDGKTSDY IYSNYEYSVE GCYRSCFQQL VLKECRCGDP
610 620 630 640 650
RFPVPENARH CDAADPIARK CLDARMNDLG GLHGSFRCRC QQPCRQSIYS
660 670 680 690 700
VTYSPAKWPS LSLQIQLGSC NGTAVECNKH YKENGAMVEV FYEQLNFEML
710 720 730 740 750
TESEAYGFVN LLADFGGQLG LWCGISFLTC CEFVFLFLET AYMSAEHNYS
760
LYKKKKAEKA KKIASGSF
Length:768
Mass (Da):87,194
Last modified:November 1, 1997 - v3
Checksum:i8899D0FCCB7F62C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53669 Genomic DNA. Translation: AAC47265.1.
FO081053 Genomic DNA. Translation: CCD68838.1.
X58982 Genomic DNA. Translation: CAA41731.1.
PIRiT29859.
RefSeqiNP_510712.2. NM_078311.5.
UniGeneiCel.634.

Genome annotation databases

EnsemblMetazoaiT01C8.7.1; T01C8.7.1; WBGene00003168.
T01C8.7.2; T01C8.7.2; WBGene00003168.
GeneIDi181728.
KEGGicel:CELE_T01C8.7.
UCSCiT01C8.7. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53669 Genomic DNA. Translation: AAC47265.1.
FO081053 Genomic DNA. Translation: CCD68838.1.
X58982 Genomic DNA. Translation: CAA41731.1.
PIRiT29859.
RefSeqiNP_510712.2. NM_078311.5.
UniGeneiCel.634.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K2BNMR-A1-103[»]
ProteinModelPortaliP24612.
SMRiP24612. Positions 1-103, 444-733.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.T01C8.7.2.

Protein family/group databases

TCDBi1.A.6.2.2. the epithelial na(+) channel (enac) family.

Proteomic databases

PaxDbiP24612.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT01C8.7.1; T01C8.7.1; WBGene00003168.
T01C8.7.2; T01C8.7.2; WBGene00003168.
GeneIDi181728.
KEGGicel:CELE_T01C8.7.
UCSCiT01C8.7. c. elegans.

Organism-specific databases

CTDi181728.
WormBaseiT01C8.7; CE39109; WBGene00003168; mec-4.

Phylogenomic databases

eggNOGiKOG4294. Eukaryota.
ENOG410ZNFK. LUCA.
GeneTreeiENSGT00760000119120.
HOGENOMiHOG000018424.
InParanoidiP24612.
OMAiQCELITG.
PhylomeDBiP24612.

Enzyme and pathway databases

ReactomeiR-CEL-2672351. Stimuli-sensing channels.

Miscellaneous databases

EvolutionaryTraceiP24612.
PROiP24612.

Family and domain databases

InterProiIPR004726. Deg-1.
IPR001873. Na+channel_ASC.
IPR020903. Na+channel_ASC_CS.
[Graphical view]
PANTHERiPTHR11690. PTHR11690. 3 hits.
PfamiPF00858. ASC. 1 hit.
[Graphical view]
PRINTSiPR01078. AMINACHANNEL.
TIGRFAMsiTIGR00867. deg-1. 1 hit.
PROSITEiPS01206. ASC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and transmembrane topology of MEC-4, an ion channel subunit required for mechanotransduction in Caenorhabditis elegans."
    Lai C.C., Hong K., Kinnell M., Chalfie M., Driscoll M.
    J. Cell Biol. 133:1071-1081(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "The mec-4 gene is a member of a family of Caenorhabditis elegans genes that can mutate to induce neuronal degeneration."
    Driscoll M., Chalfie M.
    Nature 349:588-593(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 271-768, MUTAGENESIS OF ALA-713.
    Strain: Bristol N2.
  4. "The mechanosensory protein MEC-6 is a subunit of the C. elegans touch-cell degenerin channel."
    Chelur D.S., Ernstrom G.G., Goodman M.B., Yao C.A., Chen L., O'Hagan R., Chalfie M.
    Nature 420:669-673(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INTERACTION WITH DEGENERIN CHANNEL PROTEINS.

Entry informationi

Entry nameiMEC4_CAEEL
AccessioniPrimary (citable) accession number: P24612
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: June 8, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Miscellaneous

Mutations in mec-4 results in the degeneration of a small set of neurons which typically swell to several times their normal diameter before they disappear, presumably due to lysis.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.