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P24610 (PAX3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Paired box protein Pax-3
Gene names
Name:Pax3
Synonyms:Pax-3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that may regulate cell proliferation, migration and apoptosis. Involved in neural development and myogenesis. Ref.3 Ref.4

Subunit structure

Can bind to DNA as homodimer or a heterodimer with PAX7. Interacts with DAXX By similarity. Interacts with PAXBP1; the interaction links PAX3 to a WDR5-containing histone methyltransferase complex. Ref.4

Subcellular location

Nucleus.

Developmental stage

Expressed during early neurogenesis.

Involvement in disease

The Splotch (Sp) mouse mutant displays defects in neural tube closure in the form of exencephaly and spina bifida. The splotch-delayed (Spd) phenotype is less severe than the other Sp alleles. Ref.3

Sequence similarities

Belongs to the paired homeobox family.

Contains 1 homeobox DNA-binding domain.

Contains 1 paired domain.

Ontologies

Keywords
   Biological processMyogenesis
Neurogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   DiseaseDisease mutation
   DomainHomeobox
Paired box
   LigandDNA-binding
   Molecular functionDevelopmental protein
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcardiac muscle cell differentiation

Non-traceable author statement PubMed 21556048. Source: UniProtKB

cell migration

Inferred from mutant phenotype PubMed 15132998PubMed 17974128. Source: MGI

cell proliferation

Inferred from mutant phenotype PubMed 15132998. Source: MGI

developmental pigmentation

Inferred from mutant phenotype PubMed 15322542. Source: MGI

heart development

Inferred from mutant phenotype PubMed 10231856. Source: MGI

mammary gland specification

Inferred from mutant phenotype PubMed 16720875. Source: MGI

muscle organ development

Inferred from mutant phenotype PubMed 15132998. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 15729346. Source: MGI

neural crest cell migration

Inferred from mutant phenotype PubMed 15384171. Source: MGI

neural tube closure

Inferred from mutant phenotype PubMed 15384171. Source: MGI

neural tube development

Inferred from genetic interaction PubMed 10419687. Source: MGI

neuron fate commitment

Inferred from genetic interaction PubMed 9858722. Source: MGI

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 10946068. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 15729346PubMed 16300735. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from sequence orthology PubMed 11863357. Source: MGI

regulation of somitogenesis

Inferred from mutant phenotype PubMed 17974128. Source: MGI

skeletal muscle cell differentiation

Non-traceable author statement PubMed 21556048. Source: UniProtKB

spinal cord association neuron differentiation

Inferred from genetic interaction PubMed 9858722. Source: MGI

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 15729346. Source: GOC

   Cellular_componentnucleus

Inferred from direct assay PubMed 21177767. Source: MGI

transcription factor complex

Traceable author statement PubMed 10197584. Source: MGI

   Molecular_functionRNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 15729346. Source: MGI

chromatin binding

Inferred from direct assay PubMed 15729346. Source: MGI

protein binding

Inferred from physical interaction PubMed 17662948PubMed 9464541. Source: IntAct

sequence-specific DNA binding

Inferred from direct assay PubMed 11863357. Source: MGI

sequence-specific DNA binding transcription factor activity

Traceable author statement PubMed 10197584. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PSMD4P550363EBI-1208116,EBI-359318From a different organism.
RAD23BP547274EBI-1208116,EBI-954531From a different organism.
Rb1P134053EBI-1208116,EBI-971782
RBL1P287493EBI-1208116,EBI-971402From a different organism.
RBL2Q089993EBI-1208116,EBI-971439From a different organism.
UBBP0CG472EBI-1208116,EBI-413034From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 479479Paired box protein Pax-3
PRO_0000050179

Regions

Domain34 – 161128Paired
DNA binding219 – 27860Homeobox

Amino acid modifications

Modified residue2011Phosphoserine By similarity
Modified residue2051Phosphoserine By similarity
Modified residue2091Phosphoserine By similarity

Natural variations

Natural variant421G → R in Spd. Ref.5

Experimental info

Sequence conflict1401D → G in BAC32185. Ref.2
Sequence conflict3101T → H in CAA42008. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24610 [UniParc].

Last modified March 1, 2005. Version 2.
Checksum: 8806E551FFB755A0

FASTA47952,949
        10         20         30         40         50         60 
MTTLAGAVPR MMRPGPGQNY PRSGFPLEVS TPLGQGRVNQ LGGVFINGRP LPNHIRHKIV 

        70         80         90        100        110        120 
EMAHHGIRPC VISRQLRVSH GCVSKILCRY QETGSIRPGA IGGSKPKQVT TPDVEKKIEE 

       130        140        150        160        170        180 
YKRENPGMFS WEIRDKLLKD AVCDRNTVPS VSSISRILRS KFGKGEEEEA DLERKEAEES 

       190        200        210        220        230        240 
EKKAKHSIDG ILSERASAPQ SDEGSDIDSE PDLPLKRKQR RSRTTFTAEQ LEELERAFER 

       250        260        270        280        290        300 
THYPDIYTRE ELAQRAKLTE ARVQVWFSNR RARWRKQAGA NQLMAFNHLI PGGFPPTAMP 

       310        320        330        340        350        360 
TLPTYQLSET SYQPTSIPQA VSDPSSTVHR PQPLPPSTVH QSTIPSNADS SSAYCLPSTR 

       370        380        390        400        410        420 
HGFSSYTDSF VPPSGPSNPM NPTIGNGLSP QVMGLLTNHG GVPHQPQTDY ALSPLTGGLE 

       430        440        450        460        470 
PTTTVSASCS QRLEHMKNVD SLPTSQPYCP PTYSTAGYSM DPVTGYQYGQ YGQSKPWTF 

« Hide

References

« Hide 'large scale' references
[1]"Pax-3, a novel murine DNA binding protein expressed during early neurogenesis."
Goulding M.D., Chalepakis G., Deutsch U., Erselius J.R., Gruss P.
EMBO J. 10:1135-1147(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Head.
[3]"Splotch (Sp2H), a mutation affecting development of the mouse neural tube, shows a deletion within the paired homeodomain of Pax-3."
Epstein D.J., Vekemans M., Gros P.
Cell 67:767-774(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 219-260, DISEASE, FUNCTION.
[4]"Pax3/7BP is a Pax7- and Pax3-binding protein that regulates the proliferation of muscle precursor cells by an epigenetic mechanism."
Diao Y., Guo X., Li Y., Sun K., Lu L., Jiang L., Fu X., Zhu H., Sun H., Wang H., Wu Z.
Cell Stem Cell 11:231-241(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PAXBP1.
[5]"The splotch-delayed (Spd) mouse mutant carries a point mutation within the paired box of the Pax-3 gene."
Vogan K.J., Epstein D.J., Trasler D.G., Gros P.
Genomics 17:364-369(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT SPD ARG-42.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59358 mRNA. Translation: CAA42008.1.
AK014337 mRNA. Translation: BAB29280.1.
AK045018 mRNA. Translation: BAC32185.1.
AK148354 mRNA. Translation: BAE28501.1.
S66429 mRNA. Translation: AAB20359.1.
S66433 mRNA. Translation: AAB20360.1.
CCDSCCDS15082.1.
PIRS15031.
RefSeqNP_001152992.1. NM_001159520.1.
NP_032807.3. NM_008781.4.
UniGeneMm.1371.

3D structure databases

ProteinModelPortalP24610.
SMRP24610. Positions 35-158, 219-277.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202030. 21 interactions.
IntActP24610. 7 interactions.
MINTMINT-4653013.

PTM databases

PhosphoSiteP24610.

Proteomic databases

PRIDEP24610.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000087086; ENSMUSP00000084320; ENSMUSG00000004872.
GeneID18505.
KEGGmmu:18505.
UCSCuc007bqb.2. mouse.

Organism-specific databases

CTD5077.
MGIMGI:97487. Pax3.

Phylogenomic databases

eggNOGNOG326044.
GeneTreeENSGT00680000099553.
HOGENOMHOG000230939.
HOVERGENHBG009115.
KOK09381.
PhylomeDBP24610.

Gene expression databases

BgeeP24610.
CleanExMM_PAX3.
GenevestigatorP24610.

Family and domain databases

Gene3D1.10.10.10. 2 hits.
1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001523. Paired_dom.
IPR022106. Pax7.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00292. PAX. 1 hit.
PF12360. Pax7. 1 hit.
[Graphical view]
PRINTSPR00027. PAIREDBOX.
SMARTSM00389. HOX. 1 hit.
SM00351. PAX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 2 hits.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00034. PAIRED_1. 1 hit.
PS51057. PAIRED_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPAX3. mouse.
NextBio294224.
PROP24610.
SOURCESearch...

Entry information

Entry namePAX3_MOUSE
AccessionPrimary (citable) accession number: P24610
Secondary accession number(s): Q3UFQ9, Q8BRE7, Q9CXI6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 2005
Last modified: July 9, 2014
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot