Paired box protein Pax-3 - Mus musculus (Mouse)

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P24610 (PAX3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 131. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Paired box protein Pax-3

Gene names

Name:	Pax3
Synonyms:	Pax-3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor that may regulate cell proliferation, migration and apoptosis. Involved in neural development and myogenesis. Ref.3 Ref.4
Subunit structure	Can bind to DNA as homodimer or a heterodimer with PAX7. Interacts with DAXX By similarity. Interacts with PAXBP1; the interaction links PAX3 to a WDR5-containing histone methyltransferase complex. Ref.4
Subcellular location	Nucleus.
Developmental stage	Expressed during early neurogenesis.
Involvement in disease	The Splotch (Sp) mouse mutant displays defects in neural tube closure in the form of exencephaly and spina bifida. The splotch-delayed (Spd) phenotype is less severe than the other Sp alleles. Ref.3
Sequence similarities	Belongs to the paired homeobox family. Contains 1 homeobox DNA-binding domain. Contains 1 paired domain.

Ontologies

Keywords
Biological process	Myogenesis Neurogenesis Transcription Transcription regulation
Cellular component	Nucleus
Disease	Disease mutation
Domain	Homeobox Paired box
Ligand	DNA-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiac muscle cell differentiation Inferred from expression pattern PubMed 21556048. Source: UniProtKB cell proliferation Inferred from mutant phenotype PubMed 15132998. Source: MGI developmental pigmentation Inferred from mutant phenotype PubMed 15322542. Source: MGI mammary gland specification Inferred from mutant phenotype PubMed 16720875. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction PubMed 15729346. Source: MGI neural crest cell migration Inferred from mutant phenotype PubMed 15384171. Source: MGI neural tube closure Inferred from mutant phenotype PubMed 15384171. Source: MGI neuron fate commitment Inferred from genetic interaction PubMed 9858722. Source: MGI positive regulation of cell proliferation Inferred from mutant phenotype PubMed 10946068. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 15729346 PubMed 16300735. Source: MGI skeletal muscle cell differentiation Inferred from expression pattern PubMed 21556048. Source: UniProtKB spinal cord association neuron differentiation Inferred from genetic interaction PubMed 9858722. Source: MGI
Cellular_component	transcription factor complex Traceable author statement PubMed 10197584. Source: MGI
Molecular_function	RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 15729346. Source: MGI chromatin binding Inferred from direct assay PubMed 15729346. Source: MGI sequence-specific DNA binding Inferred from direct assay PubMed 11863357. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PSMD4	P55036	3	EBI-1208116,EBI-359318	From a different organism.
RAD23B	P54727	4	EBI-1208116,EBI-954531	From a different organism.
Rb1	P13405	3	EBI-1208116,EBI-971782
RBL1	P28749	3	EBI-1208116,EBI-971402	From a different organism.
RBL2	Q08999	3	EBI-1208116,EBI-971439	From a different organism.
UBB	P0CG47	2	EBI-1208116,EBI-413034	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 479

479

Paired box protein Pax-3

PRO_0000050179

Regions

Domain

34 – 161

128

Paired

DNA binding

219 – 278

Homeobox

Amino acid modifications

Modified residue

201

Phosphoserine By similarity

Modified residue

205

Phosphoserine By similarity

Modified residue

209

Phosphoserine By similarity

Natural variations

Natural variant

G → R in Spd. Ref.5

Experimental info

Sequence conflict

140

D → G in BAC32185. Ref.2

Sequence conflict

310

T → H in CAA42008. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P24610 [UniParc].

Last modified March 1, 2005. Version 2.
Checksum: 8806E551FFB755A0
Show »

FASTA

479

52,949

        10         20         30         40         50         60 
MTTLAGAVPR MMRPGPGQNY PRSGFPLEVS TPLGQGRVNQ LGGVFINGRP LPNHIRHKIV 

        70         80         90        100        110        120 
EMAHHGIRPC VISRQLRVSH GCVSKILCRY QETGSIRPGA IGGSKPKQVT TPDVEKKIEE 

       130        140        150        160        170        180 
YKRENPGMFS WEIRDKLLKD AVCDRNTVPS VSSISRILRS KFGKGEEEEA DLERKEAEES 

       190        200        210        220        230        240 
EKKAKHSIDG ILSERASAPQ SDEGSDIDSE PDLPLKRKQR RSRTTFTAEQ LEELERAFER 

       250        260        270        280        290        300 
THYPDIYTRE ELAQRAKLTE ARVQVWFSNR RARWRKQAGA NQLMAFNHLI PGGFPPTAMP 

       310        320        330        340        350        360 
TLPTYQLSET SYQPTSIPQA VSDPSSTVHR PQPLPPSTVH QSTIPSNADS SSAYCLPSTR 

       370        380        390        400        410        420 
HGFSSYTDSF VPPSGPSNPM NPTIGNGLSP QVMGLLTNHG GVPHQPQTDY ALSPLTGGLE 

       430        440        450        460        470 
PTTTVSASCS QRLEHMKNVD SLPTSQPYCP PTYSTAGYSM DPVTGYQYGQ YGQSKPWTF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pax-3, a novel murine DNA binding protein expressed during early neurogenesis." Goulding M.D., Chalepakis G., Deutsch U., Erselius J.R., Gruss P. EMBO J. 10:1135-1147(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Head.
[3]	"Splotch (Sp2H), a mutation affecting development of the mouse neural tube, shows a deletion within the paired homeodomain of Pax-3." Epstein D.J., Vekemans M., Gros P. Cell 67:767-774(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 219-260, DISEASE, FUNCTION.
[4]	"Pax3/7BP is a Pax7- and Pax3-binding protein that regulates the proliferation of muscle precursor cells by an epigenetic mechanism." Diao Y., Guo X., Li Y., Sun K., Lu L., Jiang L., Fu X., Zhu H., Sun H., Wang H., Wu Z. Cell Stem Cell 11:231-241(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PAXBP1.
[5]	"The splotch-delayed (Spd) mouse mutant carries a point mutation within the paired box of the Pax-3 gene." Vogan K.J., Epstein D.J., Trasler D.G., Gros P. Genomics 17:364-369(1993) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT SPD ARG-42.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59358 mRNA. Translation: CAA42008.1. AK014337 mRNA. Translation: BAB29280.1. AK045018 mRNA. Translation: BAC32185.1. AK148354 mRNA. Translation: BAE28501.1. S66429 mRNA. Translation: AAB20359.1. S66433 mRNA. Translation: AAB20360.1.
IPI	IPI00625217.
PIR	S15031.
RefSeq	NP_001152992.1. NM_001159520.1. NP_032807.3. NM_008781.4.
UniGene	Mm.1371.
3D structure databases
ProteinModelPortal	P24610.
SMR	P24610. Positions 35-158, 219-277.
ModBase	Search...
Protein-protein interaction databases
IntAct	P24610. 6 interactions.
MINT	MINT-4653013.
PTM databases
PhosphoSite	P24610.
Proteomic databases
PRIDE	P24610.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000087086; ENSMUSP00000084320; ENSMUSG00000004872.
GeneID	18505.
KEGG	mmu:18505.
UCSC	uc007bqb.2. mouse.
Organism-specific databases
CTD	5077.
MGI	MGI:97487. Pax3.
Phylogenomic databases
eggNOG	NOG326044.
GeneTree	ENSGT00680000099553.
HOGENOM	HOG000230939.
HOVERGEN	HBG009115.
KO	K09381.
Gene expression databases
ArrayExpress	P24610.
Bgee	P24610.
CleanEx	MM_PAX3.
Genevestigator	P24610.
GermOnline	ENSMUSG00000004872. Mus musculus.
Family and domain databases
Gene3D	1.10.10.10. 2 hits. 1.10.10.60. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR001356. Homeodomain. IPR009057. Homeodomain-like. IPR001523. Paired_dom. IPR022106. Pax7. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. PF00292. PAX. 1 hit. PF12360. Pax7. 1 hit. [Graphical view]
PRINTS	PR00027. PAIREDBOX.
SMART	SM00389. HOX. 1 hit. SM00351. PAX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 2 hits.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. PS00034. PAIRED_1. 1 hit. PS51057. PAIRED_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PAX3. mouse.
NextBio	294224.
SOURCE	Search...

Entry information

Entry name

PAX3_MOUSE

Accession

Primary (citable) accession number: P24610
Secondary accession number(s): Q3UFQ9, Q8BRE7, Q9CXI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 2005
Last modified:	May 1, 2013
This is version 131 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents