Tyrosine-protein kinase Tec - Mus musculus (Mouse)

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Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase Tec
EC=2.7.10.2

Gene names

Name:

Tec

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	630 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	May be an important signal transducer for cell division and/or for differentiation in the liver system.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Interacts with INPP5D/SHIP1 and INPPL1/SHIP2 By similarity.
Subcellular location	Cytoplasm Probable.
Tissue specificity	Preferentially expressed in liver. Expression is also seen in the hematopoietic cells such as bone marrow, thymus and spleen. Lower expression is seen in the heart, kidney and ovary.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily. Contains 1 Btk-type zinc finger. Contains 1 PH domain. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

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Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	SH2 domain SH3 domain Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro protein kinase cascade Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: MGI zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P24604-1) Also known as: TecIV; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P24604-2) The sequence of this isoform differs from the canonical sequence as follows: 1-94: MNFNTILEEI...DANTLYIFAP → MMVSFPVKINFHS 224-245: Missing.

Isoform 3 (identifier: P24604-3) Also known as: TecIIb; The sequence of this isoform differs from the canonical sequence as follows: 604-630: RPEGRPSLEDLLRTIDELVECEETFGR → ESCLCRVAQDLSSKNLIGSRF

Isoform 4 (identifier: P24604-4) Also known as: TecIIa; The sequence of this isoform differs from the canonical sequence as follows: 224-245: Missing. 604-630: RPEGRPSLEDLLRTIDELVECEETFGR → ESCLCRVAQDLSSKNLIGSRF

Isoform 5 (identifier: P24604-5) Also known as: TecIII; The sequence of this isoform differs from the canonical sequence as follows: 224-245: Missing.

Isoform 6 (identifier: P24604-6) Also known as: TecI; The sequence of this isoform differs from the canonical sequence as follows: 82-100: VVHDANTLYIFAPSPQSRD → STKQGPMGEEVKRRNKEQQ 101-630: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 630

630

Tyrosine-protein kinase Tec

PRO_0000088171

Regions

Domain

4 – 111

108

PH

Domain

178 – 238

61

SH3

Domain

246 – 344

99

SH2

Domain

369 – 622

254

Protein kinase

Zinc finger

113 – 149

37

Btk-type

Nucleotide binding

375 – 383

9

ATP By similarity

Sites

Active site

488

1

Proton acceptor By similarity

Metal binding

121

1

Zinc By similarity

Metal binding

132

1

Zinc By similarity

Metal binding

133

1

Zinc By similarity

Metal binding

143

1

Zinc By similarity

Binding site

397

1

ATP By similarity

Amino acid modifications

Modified residue

205

1

Phosphotyrosine; by autocatalysis By similarity

Modified residue

227

1

Phosphotyrosine By similarity

Modified residue

274

1

Phosphoserine By similarity

Modified residue

518

1

Phosphotyrosine; by autocatalysis Ref.5

Natural variations

Alternative sequence

1 – 94

94

MNFNT…YIFAP → MMVSFPVKINFHS in isoform 2.

VSP_005012

Alternative sequence

82 – 100

19

VVHDA…PQSRD → STKQGPMGEEVKRRNKEQQ in isoform 6.

VSP_005013

Alternative sequence

101 – 630

530

Missing in isoform 6.

VSP_005014

Alternative sequence

224 – 245

22

Missing in isoform 2, isoform 4 and isoform 5.

VSP_005015

Alternative sequence

604 – 630

27

RPEGR…ETFGR → ESCLCRVAQDLSSKNLIGSR F in isoform 3 and isoform 4.

VSP_005016

Experimental info

Sequence conflict

23

1

L → P in AAD43404. Ref.2

Sequence conflict

23

1

L → P in AAD43402. Ref.2

Sequence conflict

23

1

L → P in AAD43405. Ref.2

Sequence conflict

23

1

L → P in AAD43406. Ref.2

Sequence conflict

23

1

L → P in AAD43407. Ref.2

Sequence conflict

31

1

C → F in AAD43404. Ref.2

Sequence conflict

31

1

C → F in AAD43402. Ref.2

Sequence conflict

31

1

C → F in AAD43405. Ref.2

Sequence conflict

31

1

C → F in AAD43406. Ref.2

Sequence conflict

31

1

C → F in AAD43407. Ref.2

Sequence conflict

34

1

P → T in AAD43404. Ref.2

Sequence conflict

34

1

P → T in AAD43402. Ref.2

Sequence conflict

34

1

P → T in AAD43405. Ref.2

Sequence conflict

34

1

P → T in AAD43406. Ref.2

Sequence conflict

34

1

P → T in AAD43407. Ref.2

Sequence conflict

535

1

V → E in AAA40018. Ref.4

Sequence conflict

550 – 553

4

FGVL → YGIP Ref.4

Sequence conflict

590

1

T → S in AAD43402. Ref.2

Sequence conflict

590

1

T → S in AAD43405. Ref.2

Sequence conflict

590

1

T → S in AAD43406. Ref.2

Sequence conflict

590

1

T → S in AAD43407. Ref.2

Sequence conflict

611

1

L → F Ref.2

Sequence conflict

611

1

L → F Ref.3

Secondary structure

1

.

630

Helix Strand Turn

Details...

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 (TecIV) [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 262640EE90D4A6D2 Show »	FASTA	630	73,426
10 20 30 40 50 60 MNFNTILEEI LIKRSQQKKK TSLLNYKERL CVLPKSVLSY YEGRAEKKYR KGVIDISKIK 70 80 90 100 110 120 CVEIVKNDDG VIPCQNKFPF QVVHDANTLY IFAPSPQSRD RWVKKLKEEI KNNNNIMIKY 130 140 150 160 170 180 HPKFWADGSY QCCRQTEKLA PGCEKYNLFE SSIRKTLPPA PEIKKRRPPP PIPPEEENTE 190 200 210 220 230 240 EIVVAMYDFQ ATEAHDLRLE RGQEYIILEK NDLHWWRARD KYGSEGYIPS NYVTGKKSNN 250 260 270 280 290 300 LDQYEWYCRN TNRSKAEQLL RTEDKEGGFM VRDSSQPGLY TVSLYTKFGG EGSSGFRHYH 310 320 330 340 350 360 IKETATSPKK YYLAEKHAFG SIPEIIEYHK HNAAGLVTRL RYPVSTKGKN APTTAGFSYD 370 380 390 400 410 420 KWEINPSELT FMRELGSGLF GVVRLGKWRA QYKVAIKAIR EGAMCEEDFI EEAKVMMKLT 430 440 450 460 470 480 HPKLVQLYGV CTQQKPIYIV TEFMERGCLL NFLRQRQGHF SRDMLLSMCQ DVCEGMEYLE 490 500 510 520 530 540 RNSFIHRDLA ARNCLVNEAG VVKVSDFGMA RYVLDDQYTS SSGAKFPVKW CPPEVFNYSR 550 560 570 580 590 600 FSSKSDVWSF GVLMWEIFTE GRMPFEKNTN YEVVTMVTRG HRLHRPKLAT KYLYEVMLRC 610 620 630 WQERPEGRPS LEDLLRTIDE LVECEETFGR « Hide
Isoform 2. Checksum: 72819611523F0872 Show »	FASTA	527	61,523
10 20 30 40 50 60 MMVSFPVKIN FHSSPQSRDR WVKKLKEEIK NNNNIMIKYH PKFWADGSYQ CCRQTEKLAP 70 80 90 100 110 120 GCEKYNLFES SIRKTLPPAP EIKKRRPPPP IPPEEENTEE IVVAMYDFQA TEAHDLRLER 130 140 150 160 170 180 GQEYIILEKN DLHWWRARDK YGWYCRNTNR SKAEQLLRTE DKEGGFMVRD SSQPGLYTVS 190 200 210 220 230 240 LYTKFGGEGS SGFRHYHIKE TATSPKKYYL AEKHAFGSIP EIIEYHKHNA AGLVTRLRYP 250 260 270 280 290 300 VSTKGKNAPT TAGFSYDKWE INPSELTFMR ELGSGLFGVV RLGKWRAQYK VAIKAIREGA 310 320 330 340 350 360 MCEEDFIEEA KVMMKLTHPK LVQLYGVCTQ QKPIYIVTEF MERGCLLNFL RQRQGHFSRD 370 380 390 400 410 420 MLLSMCQDVC EGMEYLERNS FIHRDLAARN CLVNEAGVVK VSDFGMARYV LDDQYTSSSG 430 440 450 460 470 480 AKFPVKWCPP EVFNYSRFSS KSDVWSFGVL MWEIFTEGRM PFEKNTNYEV VTMVTRGHRL 490 500 510 520 HRPKLATKYL YEVMLRCWQE RPEGRPSLED LLRTIDELVE CEETFGR « Hide
Isoform 3 (TecIIb). Checksum: 196018BC9EC862A5 Show »	FASTA	624	72,592
10 20 30 40 50 60 MNFNTILEEI LIKRSQQKKK TSLLNYKERL CVLPKSVLSY YEGRAEKKYR KGVIDISKIK 70 80 90 100 110 120 CVEIVKNDDG VIPCQNKFPF QVVHDANTLY IFAPSPQSRD RWVKKLKEEI KNNNNIMIKY 130 140 150 160 170 180 HPKFWADGSY QCCRQTEKLA PGCEKYNLFE SSIRKTLPPA PEIKKRRPPP PIPPEEENTE 190 200 210 220 230 240 EIVVAMYDFQ ATEAHDLRLE RGQEYIILEK NDLHWWRARD KYGSEGYIPS NYVTGKKSNN 250 260 270 280 290 300 LDQYEWYCRN TNRSKAEQLL RTEDKEGGFM VRDSSQPGLY TVSLYTKFGG EGSSGFRHYH 310 320 330 340 350 360 IKETATSPKK YYLAEKHAFG SIPEIIEYHK HNAAGLVTRL RYPVSTKGKN APTTAGFSYD 370 380 390 400 410 420 KWEINPSELT FMRELGSGLF GVVRLGKWRA QYKVAIKAIR EGAMCEEDFI EEAKVMMKLT 430 440 450 460 470 480 HPKLVQLYGV CTQQKPIYIV TEFMERGCLL NFLRQRQGHF SRDMLLSMCQ DVCEGMEYLE 490 500 510 520 530 540 RNSFIHRDLA ARNCLVNEAG VVKVSDFGMA RYVLDDQYTS SSGAKFPVKW CPPEVFNYSR 550 560 570 580 590 600 FSSKSDVWSF GVLMWEIFTE GRMPFEKNTN YEVVTMVTRG HRLHRPKLAT KYLYEVMLRC 610 620 WQEESCLCRV AQDLSSKNLI GSRF « Hide
Isoform 4 (TecIIa). Checksum: B213B18A8519E4F5 Show »	FASTA	602	70,104
10 20 30 40 50 60 MNFNTILEEI LIKRSQQKKK TSLLNYKERL CVLPKSVLSY YEGRAEKKYR KGVIDISKIK 70 80 90 100 110 120 CVEIVKNDDG VIPCQNKFPF QVVHDANTLY IFAPSPQSRD RWVKKLKEEI KNNNNIMIKY 130 140 150 160 170 180 HPKFWADGSY QCCRQTEKLA PGCEKYNLFE SSIRKTLPPA PEIKKRRPPP PIPPEEENTE 190 200 210 220 230 240 EIVVAMYDFQ ATEAHDLRLE RGQEYIILEK NDLHWWRARD KYGWYCRNTN RSKAEQLLRT 250 260 270 280 290 300 EDKEGGFMVR DSSQPGLYTV SLYTKFGGEG SSGFRHYHIK ETATSPKKYY LAEKHAFGSI 310 320 330 340 350 360 PEIIEYHKHN AAGLVTRLRY PVSTKGKNAP TTAGFSYDKW EINPSELTFM RELGSGLFGV 370 380 390 400 410 420 VRLGKWRAQY KVAIKAIREG AMCEEDFIEE AKVMMKLTHP KLVQLYGVCT QQKPIYIVTE 430 440 450 460 470 480 FMERGCLLNF LRQRQGHFSR DMLLSMCQDV CEGMEYLERN SFIHRDLAAR NCLVNEAGVV 490 500 510 520 530 540 KVSDFGMARY VLDDQYTSSS GAKFPVKWCP PEVFNYSRFS SKSDVWSFGV LMWEIFTEGR 550 560 570 580 590 600 MPFEKNTNYE VVTMVTRGHR LHRPKLATKY LYEVMLRCWQ EESCLCRVAQ DLSSKNLIGS RF « Hide
Isoform 5 (TecIII). Checksum: C43EF410FD030DA1 Show »	FASTA	608	70,938
10 20 30 40 50 60 MNFNTILEEI LIKRSQQKKK TSLLNYKERL CVLPKSVLSY YEGRAEKKYR KGVIDISKIK 70 80 90 100 110 120 CVEIVKNDDG VIPCQNKFPF QVVHDANTLY IFAPSPQSRD RWVKKLKEEI KNNNNIMIKY 130 140 150 160 170 180 HPKFWADGSY QCCRQTEKLA PGCEKYNLFE SSIRKTLPPA PEIKKRRPPP PIPPEEENTE 190 200 210 220 230 240 EIVVAMYDFQ ATEAHDLRLE RGQEYIILEK NDLHWWRARD KYGWYCRNTN RSKAEQLLRT 250 260 270 280 290 300 EDKEGGFMVR DSSQPGLYTV SLYTKFGGEG SSGFRHYHIK ETATSPKKYY LAEKHAFGSI 310 320 330 340 350 360 PEIIEYHKHN AAGLVTRLRY PVSTKGKNAP TTAGFSYDKW EINPSELTFM RELGSGLFGV 370 380 390 400 410 420 VRLGKWRAQY KVAIKAIREG AMCEEDFIEE AKVMMKLTHP KLVQLYGVCT QQKPIYIVTE 430 440 450 460 470 480 FMERGCLLNF LRQRQGHFSR DMLLSMCQDV CEGMEYLERN SFIHRDLAAR NCLVNEAGVV 490 500 510 520 530 540 KVSDFGMARY VLDDQYTSSS GAKFPVKWCP PEVFNYSRFS SKSDVWSFGV LMWEIFTEGR 550 560 570 580 590 600 MPFEKNTNYE VVTMVTRGHR LHRPKLATKY LYEVMLRCWQ ERPEGRPSLE DLLRTIDELV ECEETFGR « Hide
Isoform 6 (TecI). Checksum: 32AB0BC0567F2CEC Show »	FASTA	100	11,723
10 20 30 40 50 60 MNFNTILEEI LIKRSQQKKK TSLLNYKERL CVLPKSVLSY YEGRAEKKYR KGVIDISKIK 70 80 90 100 CVEIVKNDDG VIPCQNKFPF QSTKQGPMGE EVKRRNKEQQ « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of a novel form of Tec kinase in hematopoietic cells and mapping of the gene to chromosome 5 near Kit." Mano H., Mano K., Tang B., Koehler M., Yi T., Gilbert D.J., Jenkins N.A., Copeland N.G., Ihle J.N. Oncogene 8:417-424(1993) [PubMed: 7678927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Splice variants of the mouse Tec gene are differentially expressed in vivo." Merkel A.L., Atmosukarto I.I.C., Stevens K., Rathjen P.D., Booker G.W. Cytogenet. Cell Genet. 84:132-139(1999) [PubMed: 10343129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 3; 4; 5 AND 6). Strain: 129.
[3]	"A novel protein-tyrosine kinase, tec, is preferentially expressed in liver." Mano H., Ishikawa F., Nishida J., Hirai H., Takaku F. Oncogene 5:1781-1786(1990) [PubMed: 2284097] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 82-630 (ISOFORM 2). Strain: BALB/c. Tissue: Liver.
[4]	"The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family." Wilks A.F., Kurban R.R., Hovens C.M., Ralph S.J. Gene 85:67-74(1989) [PubMed: 2482828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 485-553.
[5]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-518, MASS SPECTROMETRY. Tissue: Mast cell.
[6]	"The solution structure and intramolecular associations of the Tec kinase SRC homology 3 domain." Pursglove S.E., Mulhern T.D., Mackay J.P., Hinds M.G., Booker G.W. J. Biol. Chem. 277:755-762(2002) [PubMed: 11684687] [Abstract] Cited for: STRUCTURE BY NMR OF 179-245.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ

S53716 mRNA. Translation: AAA13515.2.
AF071938, AF071936, AF071937 Genomic DNA. Translation: AAD43404.1.
AF071946

AF071945 Genomic DNA. Translation: AAD43402.1.
AF071946

AF071945 Genomic DNA. Translation: AAD43405.1.
AF071946

AF071945 Genomic DNA. Translation: AAD43406.1.
AF071946

AF071945 Genomic DNA. Translation: AAD43407.1.
X55663 mRNA. Translation: CAA39196.1.
M33427 mRNA. Translation: AAA40018.1.

IPI

IPI00108628.
IPI00227790.
IPI00227791.
IPI00227792.
IPI00227793.
IPI00227794.

PIR

JU0215.
S13763.
T01380.

RefSeq

NP_001106931.1.
NP_001106932.1.
NP_001106935.1.

UniGene

Mm.319581

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GL5	NMR	-	A	181-244	[»]

SMR

P24604. Positions 5-145, 154-236, 180-627.

ModBase

Search...

Protein-protein interaction databases

STRING

P24604.

PTM databases

PhosphoSite

P24604.

Proteomic databases

PRIDE

P24604.

Genome annotation databases

Ensembl

ENSMUST00000071944; ENSMUSP00000071836; ENSMUSG00000029217; Mus musculus. [Genome view]
ENSMUST00000073843; ENSMUSP00000073509; ENSMUSG00000029217; Mus musculus. [Genome view]
ENSMUST00000113593; ENSMUSP00000109223; ENSMUSG00000029217; Mus musculus. [Genome view]
ENSMUST00000113594; ENSMUSP00000109224; ENSMUSG00000029217; Mus musculus. [Genome view]

GeneID

21682.

KEGG

mmu:21682.

UCSC

uc008xsc.1. mouse.

Organism-specific databases

CTD

21682.

MGI

MGI:98662. Tec.

Phylogenomic databases

eggNOG

roNOG13967.

HOGENOM

HBG755340.

HOVERGEN

P24604.

InParanoid

P24604.

Enzyme and pathway databases

BRENDA

2.7.10.2. 244.

Gene expression databases

ArrayExpress

P24604.

Bgee

P24604.

Genevestigator

P24604.

GermOnline

ENSMUSG00000029217. Mus musculus.

Family and domain databases

InterPro

IPR011009. Kinase-like_dom.
IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR020473. SH3_region.
IPR020755. Tyr_kinase_non-rcpt_Txk/Tec.
IPR020635. Tyr_Pkinase_cat_dom.
IPR020685. Tyr_prot_kinase.
IPR008266. Tyr_prot_kinase_AS.
IPR001562. Znf_Btk_motif.
[Graphical view]

Gene3D

G3DSA:2.30.29.30. PH_type. 1 hit.
G3DSA:3.30.505.10. SH2. 1 hit.

PANTHER

PTHR23256:SF263. Tyr_kinase_non-rcpt_Txk/Tec. 1 hit.
PTHR23256. Tyr_prot_kinase. 1 hit.

Pfam

PF00779. BTK. 1 hit.
PF00169. PH. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

PRINTS

PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00402. TECBTKDOMAIN.

SMART

SM00107. BTK. 1 hit.
SM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]

PROSITE

PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]

ProtoNet

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Other Resources

SOURCE

Search...

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Entry information

Entry name

TEC_MOUSE

Accession

Primary (citable) accession number: P24604
Secondary accession number(s): Q9R1M9

Q9WVN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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