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Protein

Bacterioferritin

Gene

bfr

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Iron-storage protein, whose ferroxidase center binds Fe2+ ions, oxidizes them by dioxygen to Fe3+, and participates in the subsequent Fe3+ oxide mineral core formation within the central cavity of the protein complex.

Catalytic activityi

4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bCuratedNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.Curated
  • Fe cationBy similarityNote: Binds 2 iron ions per subunit. The catalytic dinuclear iron-binding site within each subunit is known as the ferroxidase center.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi18 – 181Iron 1PROSITE-ProRule annotation
Metal bindingi48 – 481Iron (heme axial ligand); shared with dimeric partnerPROSITE-ProRule annotation
Metal bindingi51 – 511Iron 1PROSITE-ProRule annotation
Metal bindingi51 – 511Iron 2PROSITE-ProRule annotation
Metal bindingi54 – 541Iron 1PROSITE-ProRule annotation
Metal bindingi94 – 941Iron 2PROSITE-ProRule annotation
Metal bindingi128 – 1281Iron 1PROSITE-ProRule annotation
Metal bindingi128 – 1281Iron 2PROSITE-ProRule annotation
Metal bindingi131 – 1311Iron 2PROSITE-ProRule annotation

GO - Molecular functioni

  1. ferric iron binding Source: InterPro
  2. ferroxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular iron ion homeostasis Source: UniProtKB-KW
  2. iron ion transport Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Iron storage

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bacterioferritin (EC:1.16.3.1)
Short name:
BFR
Gene namesi
Name:bfr
Ordered Locus Names:sll1341
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 156156BacterioferritinPRO_0000192613Add
BLAST

Proteomic databases

PaxDbiP24602.

Interactioni

Subunit structurei

Homooligomer of 24 subunits, arranged as 12 dimers, that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be deposited.By similarity

Protein-protein interaction databases

STRINGi1148.sll1341.

Structurei

3D structure databases

ProteinModelPortaliP24602.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 146146Ferritin-like diironPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the bacterioferritin family.Curated
Contains 1 ferritin-like diiron domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2193.
HOGENOMiHOG000262383.
InParanoidiP24602.
KOiK03594.
OMAiAIDQYFI.
OrthoDBiEOG6WDSKP.
PhylomeDBiP24602.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
InterProiIPR002024. Bacterioferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PfamiPF00210. Ferritin. 1 hit.
[Graphical view]
PIRSFiPIRSF002560. Bacterioferritin. 1 hit.
PRINTSiPR00601. BACFERRITIN.
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR00754. bfr. 1 hit.
PROSITEiPS00549. BACTERIOFERRITIN. 1 hit.
PS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24602-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGKPAVLAQ LHKLLRGELA ARDQYFIHSR MYQDWGLEKL YSRIDHEMQD
60 70 80 90 100
ETAHASLLIE RILFLEETPD LSQQDPIRVG KTVPEMLQYD LDYEYEVIAN
110 120 130 140 150
LKEAMAVCEQ EQDYQSRDLL LKILADTEED HAYWLEKQLG LIEKIGLQNY

LQSQMS
Length:156
Mass (Da):18,331
Last modified:November 1, 1997 - v2
Checksum:i7C1A13B31157AF86
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 542AH → HA AA sequence (PubMed:1536655).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18637.1.
PIRiS76725.
RefSeqiNP_442825.1. NC_000911.1.
YP_005652886.1. NC_017277.1.
YP_007452701.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA18637; BAA18637; BAA18637.
KEGGisyn:sll1341.
PATRICi23843424. VBISynSp132158_3248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18637.1.
PIRiS76725.
RefSeqiNP_442825.1. NC_000911.1.
YP_005652886.1. NC_017277.1.
YP_007452701.1. NC_020286.1.

3D structure databases

ProteinModelPortaliP24602.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1148.sll1341.

Proteomic databases

PaxDbiP24602.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA18637; BAA18637; BAA18637.
KEGGisyn:sll1341.
PATRICi23843424. VBISynSp132158_3248.

Phylogenomic databases

eggNOGiCOG2193.
HOGENOMiHOG000262383.
InParanoidiP24602.
KOiK03594.
OMAiAIDQYFI.
OrthoDBiEOG6WDSKP.
PhylomeDBiP24602.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
InterProiIPR002024. Bacterioferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PfamiPF00210. Ferritin. 1 hit.
[Graphical view]
PIRSFiPIRSF002560. Bacterioferritin. 1 hit.
PRINTSiPR00601. BACFERRITIN.
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR00754. bfr. 1 hit.
PROSITEiPS00549. BACTERIOFERRITIN. 1 hit.
PS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.
  2. "Purification, characterization and function of bacterioferritin from the cyanobacterium Synechocystis P.C.C. 6803."
    Laulhere J.-P., Laboure A.-M., van Wuytswinkel O., Gagnon J., Briat J.-F.
    Biochem. J. 281:785-793(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-54.

Entry informationi

Entry nameiBFR_SYNY3
AccessioniPrimary (citable) accession number: P24602
Secondary accession number(s): P74531
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: April 1, 2015
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.