P24601 (METB_HERAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cystathionine gamma-synthase
Short name=CGS
EC=2.5.1.48

Alternative name(s):
O-succinylhomoserine (thiol)-lyase

Gene names

Name:

metB

Organism

Herpetosiphon aurantiacus (Herpetosiphon giganteus)

Taxonomic identifier

65 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Herpetosiphonales › Herpetosiphonaceae › Herpetosiphon

Protein attributes

Sequence length	319 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cystathionine gamma-synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›319	›319	Probable cystathionine gamma-synthase		PRO_0000114760
Amino acid modifications
	Modified residue	197	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Non-terminal residue	319	1

Sequences

	Sequence		Length	Mass (Da)
	P24601 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 8C7D14992CC7BEBD	FASTA	319	34,685
	10 20 30 40 50 60 MDYGFATRAI HAGQDPESVT GAVIVPIYQT STYAQRGVGD HTGYEYSRTD NPTRTALQTC 70 80 90 100 110 120 LAALEEAKHA LVFASGLGAS TTLMLMLKAG DHVICGDDVY GGTYRLFQRV MTEHGLSFDF 130 140 150 160 170 180 IDMADPEAVR AAIKPNTRLI WLETPTNPLL KLAPIAAITR VAREHGIWTI VDNTFASPYN 190 200 210 220 230 240 QRPITLGADM VLHSTTKYIG GHSDVVGGAI MTSNDELYEK LKFLQNAAGA VPGPFDCWLV 250 260 270 280 290 300 LRGVKTLSIR MRDDERNALA IAQFLTEHPG VEKVIYPGLP SHPQHNLARE QMRGFGGMIS 310 ILLKGGAEAA NAMVSKTKL

References

[1]	"Stepwise cloning and molecular characterization of the HgiDI restriction-modification system from Herpetosiphon giganteus Hpa2." Duesterhoeft A., Erdmann D., Kroeger M. Nucleic Acids Res. 19:1049-1056(1991) [PubMed: 2020544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HPA2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55140 Genomic DNA. Translation: CAA38939.1.
PIR	S14030.
3D structure databases
ProteinModelPortal	P24601.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METB_HERAU

Accession

Primary (citable) accession number: P24601

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	September 21, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program