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P24600

- MTD1_HERAU

UniProt

P24600 - MTD1_HERAU

Protein

Modification methylase HgiDI

Gene

hgiDIM

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    • Comment

    Functioni

    This methylase recognizes the double-stranded sequence GRCGYC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HgiDI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei75 – 751PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3417. M.HgiDI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase HgiDI (EC:2.1.1.37)
    Short name:
    M.HgiDI
    Alternative name(s):
    Cytosine-specific methyltransferase HgiDI
    Gene namesi
    Name:hgiDIM
    OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
    Taxonomic identifieri65 [NCBI]
    Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Modification methylase HgiDIPRO_0000087888Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP24600.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 297297SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 2 hits.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24600-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTIDLFAGC GGMSLGFMQA GFEIVAAVDN WRPAINTYQQ NFTHPIHELD    50
    LAQIDAAVSL IKTHSPELII GGPPCQDFSS AGKRDEGLGR ANLTLDFAKI 100
    VLAIQPAWVI MENVERARLS KIHQQACSML GDEGYSLAQV VLDASLCGVP 150
    QLRKRTFVIG HRHGSIADLA NVLQQRLAKQ SLTVRDYFGE SLDTDYYYRH 200
    PRTYERRAIF SVNEPSPTIR GVNRPIPATY RMHPKDAGDV SLARPLTTKE 250
    RSLIQTFPLD FKFVGTKSEQ EQMIGNAVPV NLAFFLATSL QAYLNQPRMQ 300
    QLSLLPSFF 309
    Length:309
    Mass (Da):34,439
    Last modified:March 1, 1992 - v1
    Checksum:i74F29BFBF13E142F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55140 Genomic DNA. Translation: CAA38938.1.
    PIRiS14029.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55140 Genomic DNA. Translation: CAA38938.1 .
    PIRi S14029.

    3D structure databases

    ProteinModelPortali P24600.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3417. M.HgiDI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 2 hits.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Stepwise cloning and molecular characterization of the HgiDI restriction-modification system from Herpetosiphon giganteus Hpa2."
      Duesterhoeft A., Erdmann D., Kroeger M.
      Nucleic Acids Res. 19:1049-1056(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HPA2.
    2. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
      Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
      Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISCUSSION OF SEQUENCE.

    Entry informationi

    Entry nameiMTD1_HERAU
    AccessioniPrimary (citable) accession number: P24600
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3