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Protein

Modification methylase HgiDI

Gene

hgiDIM

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GRCGYC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HgiDI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei75PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3417. M.HgiDI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HgiDI (EC:2.1.1.37)
Short name:
M.HgiDI
Alternative name(s):
Cytosine-specific methyltransferase HgiDI
Gene namesi
Name:hgiDIM
OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifieri65 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878881 – 309Modification methylase HgiDIAdd BLAST309

Structurei

3D structure databases

ProteinModelPortaliP24600.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 297SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST297

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR031303. C5_meth_CS.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF00145. DNA_methylase. 2 hits.
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiView protein in PROSITE
PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.

Sequencei

Sequence statusi: Complete.

P24600-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTIDLFAGC GGMSLGFMQA GFEIVAAVDN WRPAINTYQQ NFTHPIHELD
60 70 80 90 100
LAQIDAAVSL IKTHSPELII GGPPCQDFSS AGKRDEGLGR ANLTLDFAKI
110 120 130 140 150
VLAIQPAWVI MENVERARLS KIHQQACSML GDEGYSLAQV VLDASLCGVP
160 170 180 190 200
QLRKRTFVIG HRHGSIADLA NVLQQRLAKQ SLTVRDYFGE SLDTDYYYRH
210 220 230 240 250
PRTYERRAIF SVNEPSPTIR GVNRPIPATY RMHPKDAGDV SLARPLTTKE
260 270 280 290 300
RSLIQTFPLD FKFVGTKSEQ EQMIGNAVPV NLAFFLATSL QAYLNQPRMQ

QLSLLPSFF
Length:309
Mass (Da):34,439
Last modified:March 1, 1992 - v1
Checksum:i74F29BFBF13E142F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55140 Genomic DNA. Translation: CAA38938.1.
PIRiS14029.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMTD1_HERAU
AccessioniPrimary (citable) accession number: P24600
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: May 10, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families