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P24600 (MTD1_HERAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase HgiDI

Short name=M.HgiDI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase HgiDI
Gene names
Name:hgiDIM
OrganismHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifier65 [NCBI]
Taxonomic lineageBacteriaChloroflexiHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GRCGYC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HgiDI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Modification methylase HgiDI
PRO_0000087888

Regions

Domain1 – 297297SAM-dependent MTase C5-type

Sites

Active site751 By similarity

Sequences

Sequence LengthMass (Da)Tools
P24600 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 74F29BFBF13E142F

FASTA30934,439
        10         20         30         40         50         60 
MKTIDLFAGC GGMSLGFMQA GFEIVAAVDN WRPAINTYQQ NFTHPIHELD LAQIDAAVSL 

        70         80         90        100        110        120 
IKTHSPELII GGPPCQDFSS AGKRDEGLGR ANLTLDFAKI VLAIQPAWVI MENVERARLS 

       130        140        150        160        170        180 
KIHQQACSML GDEGYSLAQV VLDASLCGVP QLRKRTFVIG HRHGSIADLA NVLQQRLAKQ 

       190        200        210        220        230        240 
SLTVRDYFGE SLDTDYYYRH PRTYERRAIF SVNEPSPTIR GVNRPIPATY RMHPKDAGDV 

       250        260        270        280        290        300 
SLARPLTTKE RSLIQTFPLD FKFVGTKSEQ EQMIGNAVPV NLAFFLATSL QAYLNQPRMQ 


QLSLLPSFF 

« Hide

References

[1]"Stepwise cloning and molecular characterization of the HgiDI restriction-modification system from Herpetosiphon giganteus Hpa2."
Duesterhoeft A., Erdmann D., Kroeger M.
Nucleic Acids Res. 19:1049-1056(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HPA2.
[2]"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55140 Genomic DNA. Translation: CAA38938.1.
PIRS14029.

3D structure databases

ProteinModelPortalP24600.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3417. M.HgiDI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 2 hits.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTD1_HERAU
AccessionPrimary (citable) accession number: P24600
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries