P24599 (T2D1_HERAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme HgiDI
Short name=R.HgiDI
EC=3.1.21.4

Alternative name(s):
Endonuclease HgiDI
Type II restriction enzyme HgiDI

Gene names

Name:

hgiDIR

Organism

Herpetosiphon aurantiacus (Herpetosiphon giganteus)

Taxonomic identifier

65 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Chloroflexi › Herpetosiphonales › Herpetosiphonaceae › Herpetosiphon

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence GRCGYC and cleaves after R-2.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Type-2 restriction enzyme HgiDI		PRO_0000077313

Sequences

	Sequence		Length	Mass (Da)
	P24599 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 67C9B01B88B6890C	FASTA	359	41,204
	10 20 30 40 50 60 MFEQLNQPGL FGITNSNRDF TKKEAWGKNQ FNNAFPIALA CFMFSQNIKP IYIRLEKRNI 70 80 90 100 110 120 EHNYFAVDQV FQINPLEAQA FFAFEHSYHP YTELIIGKTP AIDVVISNLQ NSQIINAFEI 130 140 150 160 170 180 KLTAIPDNTT ANLPDNLQGC EIVIRPDTIV YLALSIAKVF QQNPLALLDI LDPVCARIGD 190 200 210 220 230 240 WEDATSIQPM IPLFCELLYT IFDRYQAVQI PILLQPIWKT QGKLSILHEN CLDLFVWSNF 250 260 270 280 290 300 ALAKVFLDAS IKPSEKSITR PERTTVWLIK MLYDFAQNGK IDYKRTLDRI TFNLKNDKAF 310 320 330 340 350 AASGMVTRKY MNSPELQNPR IKRHSIKHII INGGQRYLSP ERRLDSAIVS TPGLFEEIL

References

[1]	"Stepwise cloning and molecular characterization of the HgiDI restriction-modification system from Herpetosiphon giganteus Hpa2." Duesterhoeft A., Erdmann D., Kroeger M. Nucleic Acids Res. 19:1049-1056(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HPA2.
[2]	"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus." Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D. Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55140 Genomic DNA. Translation: CAA38937.1.
PIR	S14028.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1102. HgiDI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019044. Restrct_endonuc_II_HindVP. [Graphical view]
Pfam	PF09519. RE_HindVP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2D1_HERAU

Accession

Primary (citable) accession number: P24599

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program