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P24599

- T2D1_HERAU

UniProt

P24599 - T2D1_HERAU

Protein

Type-2 restriction enzyme HgiDI

Gene

hgiDIR

Organism
Herpetosiphon aurantiacus (Herpetosiphon giganteus)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 57 (03 Sep 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GRCGYC and cleaves after R-2.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1102. HgiDI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme HgiDI (EC:3.1.21.4)
    Short name:
    R.HgiDI
    Alternative name(s):
    Endonuclease HgiDI
    Type II restriction enzyme HgiDI
    Gene namesi
    Name:hgiDIR
    OrganismiHerpetosiphon aurantiacus (Herpetosiphon giganteus)
    Taxonomic identifieri65 [NCBI]
    Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 359359Type-2 restriction enzyme HgiDIPRO_0000077313Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR019044. Restrct_endonuc_II_HindVP.
    [Graphical view]
    PfamiPF09519. RE_HindVP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24599-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFEQLNQPGL FGITNSNRDF TKKEAWGKNQ FNNAFPIALA CFMFSQNIKP    50
    IYIRLEKRNI EHNYFAVDQV FQINPLEAQA FFAFEHSYHP YTELIIGKTP 100
    AIDVVISNLQ NSQIINAFEI KLTAIPDNTT ANLPDNLQGC EIVIRPDTIV 150
    YLALSIAKVF QQNPLALLDI LDPVCARIGD WEDATSIQPM IPLFCELLYT 200
    IFDRYQAVQI PILLQPIWKT QGKLSILHEN CLDLFVWSNF ALAKVFLDAS 250
    IKPSEKSITR PERTTVWLIK MLYDFAQNGK IDYKRTLDRI TFNLKNDKAF 300
    AASGMVTRKY MNSPELQNPR IKRHSIKHII INGGQRYLSP ERRLDSAIVS 350
    TPGLFEEIL 359
    Length:359
    Mass (Da):41,204
    Last modified:March 1, 1992 - v1
    Checksum:i67C9B01B88B6890C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55140 Genomic DNA. Translation: CAA38937.1.
    PIRiS14028.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55140 Genomic DNA. Translation: CAA38937.1 .
    PIRi S14028.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1102. HgiDI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019044. Restrct_endonuc_II_HindVP.
    [Graphical view ]
    Pfami PF09519. RE_HindVP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Stepwise cloning and molecular characterization of the HgiDI restriction-modification system from Herpetosiphon giganteus Hpa2."
      Duesterhoeft A., Erdmann D., Kroeger M.
      Nucleic Acids Res. 19:1049-1056(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HPA2.
    2. "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
      Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
      Gene 157:43-47(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISCUSSION OF SEQUENCE.

    Entry informationi

    Entry nameiT2D1_HERAU
    AccessioniPrimary (citable) accession number: P24599
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: September 3, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3