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Reviewed, UniProtKB/Swiss-Prot P24599 (T2D1_HERAU)

Last modified June 16, 2009. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Type-2 restriction enzyme HgiDI
      Short name=R.HgiDI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme HgiDI
    Endonuclease HgiDI
Gene names
Name: hgiDIR
OrganismHerpetosiphon aurantiacus (Herpetosiphon giganteus)
Taxonomic identifier65 [NCBI]
Taxonomic lineageBacteriaChloroflexiHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Protein existencePredicted.

General annotation (Comments)

Function

Recognizes the double-stranded sequence GRCGYC and cleaves after R-2.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 359359Type-2 restriction enzyme HgiDI
PRO_0000077313

Sequences

Sequence LengthMass (Da)Tools
P24599-1 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 67C9B01B88B6890C

FASTA35941,204
        10         20         30         40         50         60 
MFEQLNQPGL FGITNSNRDF TKKEAWGKNQ FNNAFPIALA CFMFSQNIKP IYIRLEKRNI 

        70         80         90        100        110        120 
EHNYFAVDQV FQINPLEAQA FFAFEHSYHP YTELIIGKTP AIDVVISNLQ NSQIINAFEI 

       130        140        150        160        170        180 
KLTAIPDNTT ANLPDNLQGC EIVIRPDTIV YLALSIAKVF QQNPLALLDI LDPVCARIGD 

       190        200        210        220        230        240 
WEDATSIQPM IPLFCELLYT IFDRYQAVQI PILLQPIWKT QGKLSILHEN CLDLFVWSNF 

       250        260        270        280        290        300 
ALAKVFLDAS IKPSEKSITR PERTTVWLIK MLYDFAQNGK IDYKRTLDRI TFNLKNDKAF 

       310        320        330        340        350 
AASGMVTRKY MNSPELQNPR IKRHSIKHII INGGQRYLSP ERRLDSAIVS TPGLFEEIL 

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References

[1]"Stepwise cloning and molecular characterization of the HgiDI restriction-modification system from Herpetosiphon giganteus Hpa2."
Duesterhoeft A., Erdmann D., Kroeger M.
Nucleic Acids Res. 19:1049-1056(1991) [PubMed: 2020544] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HPA2.
[2]"Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."
Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.
Gene 157:43-47(1995) [PubMed: 7607523] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55140 Genomic DNA. Translation: CAA38937.1.
PIRS14028.

3D structure databases

ModBaseSearch...

Protein family/group databases

REBASE1102. HgiDI.

Enzyme and pathway databases

BRENDA3.1.21.4. 291850.

Family and domain databases

InterProIPR019044. Restrict_endonuc_II_HindVP.
[Graphical view]
PfamPF09519. RE_HindVP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2D1_HERAU
AccessionPrimary (citable) accession number: P24599
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents