P24594 (IBP5_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like growth factor-binding protein 5
Short name=IBP-5
Short name=IGF-binding protein 5
Short name=IGFBP-5

Gene names

Name:	Igfbp5
Synonyms:	Igfbp-5

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Subcellular location	Secreted.
Tissue specificity	Mostly in kidney.
Sequence similarities	Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Growth factor binding
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to cAMP Inferred from electronic annotation. Source: Compara glucose homeostasis Inferred from electronic annotation. Source: Compara glucose metabolic process Inferred from electronic annotation. Source: Compara hair follicle morphogenesis Inferred from electronic annotation. Source: Compara intracellular signal transduction Inferred from expression pattern PubMed 12193414. Source: RGD mammary gland involution Inferred from electronic annotation. Source: Compara negative regulation of insulin-like growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of osteoblast differentiation Inferred from electronic annotation. Source: Compara negative regulation of smooth muscle cell migration Inferred from electronic annotation. Source: Compara negative regulation of smooth muscle cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of translation Inferred from electronic annotation. Source: Compara osteoblast differentiation Inferred from electronic annotation. Source: Compara positive regulation of insulin-like growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of protein kinase B signaling cascade Inferred from electronic annotation. Source: Compara regulation of cell growth Inferred from electronic annotation. Source: Compara regulation of glucose metabolic process Inferred from electronic annotation. Source: Compara skeletal muscle tissue growth Inferred from expression pattern PubMed 12397024. Source: RGD striated muscle cell differentiation Inferred from electronic annotation. Source: Compara type B pancreatic cell proliferation Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	insulin-like growth factor binding Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 271	252	Insulin-like growth factor-binding protein 5		PRO_0000014388
Regions
	Domain	22 – 102	81	IGFBP N-terminal
	Domain	188 – 262	75	Thyroglobulin type-1
Amino acid modifications
	Disulfide bond	44 ↔ 58		By similarity
	Disulfide bond	66 ↔ 79		By similarity
	Disulfide bond	191 ↔ 218		By similarity
	Disulfide bond	229 ↔ 240		By similarity
	Disulfide bond	242 ↔ 262		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24594 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 545B4C48F9BB5493	FASTA	271	30,298
	10 20 30 40 50 60 MVISVVLLLL AACAVPAQGL GSFVHCEPCD EKALSMCPPS PLGCELVKEP GCGCCMTCAL 70 80 90 100 110 120 AEGQSCGVYT ERCAQGLRCL PRQDEEKPLH ALLHGRGVCL NEKSYGEQTK IERDSREHEE 130 140 150 160 170 180 PTTSEMAEET YSPKVFRPKH TRISELKAEA VKKDRRKKLT QSKFVGGAEN TAHPRVIPAP 190 200 210 220 230 240 EMRQESDQGP CRRHMEASLQ EFKASPRMVP RAVYLPNCDR KGFYKRKQCK PSRGRKRGIC 250 260 270 WCVDKYGMKL PGMEYVDGDF QCHAFDSSNV E

References

[1]	"Identification of five different insulin-like growth factor binding proteins (IGFBPs) from adult rat serum and molecular cloning of a novel IGFBP-5 in rat and human." Shimasaki S., Shimonaka M., Zhang H.-P., Ling N. J. Biol. Chem. 266:10646-10653(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-53. Tissue: Ovary.
[2]	"Cloning of the rat insulin-like growth factor binding protein-5 gene and DNA sequence analysis of its promoter region." Zhu X., Ling N., Shimasaki S. Biochem. Biophys. Res. Commun. 190:1045-1052(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M62781 mRNA. Translation: AAA53533.1. L08275 Genomic DNA. No translation available.
IPI	IPI00215485.
PIR	JC1463.
RefSeq	NP_036949.1. NM_012817.1.
UniGene	Rn.1593.
3D structure databases
ProteinModelPortal	P24594.
SMR	P24594. Positions 24-104.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-204226.
STRING	10116.ENSRNOP00000023530.
Protein family/group databases
MEROPS	I31.952.
Proteomic databases
PRIDE	P24594.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000023530; ENSRNOP00000023530; ENSRNOG00000017206.
GeneID	25285.
KEGG	rno:25285.
UCSC	RGD:2876. rat.
Organism-specific databases
CTD	3488.
RGD	2876. Igfbp5.
Phylogenomic databases
eggNOG	NOG42882.
GeneTree	ENSGT00550000074457.
HOGENOM	HOG000253012.
HOVERGEN	HBG002631.
InParanoid	P24594.
OMA	HEPEFGP.
OrthoDB	EOG4RV2RZ.
Gene expression databases
Genevestigator	P24594.
GermOnline	ENSRNOG00000017206. Rattus norvegicus.
Family and domain databases
Gene3D	4.10.800.10. 1 hit.
InterPro	IPR009030. Growth_fac_rcpt. IPR012213. IGFBP-5. IPR000867. IGFBP-like. IPR009168. IGFBP1-6. IPR022321. IGFBP_1-6_chordata. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000716. Thyroglobulin_1. [Graphical view]
PANTHER	PTHR11551. PTHR11551. 1 hit. PTHR11551:SF4. PTHR11551:SF4. 1 hit.
Pfam	PF00219. IGFBP. 1 hit. PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
PRINTS	PR01976. IGFBPFAMILY. PR01981. IGFBPFAMILY5.
SMART	SM00121. IB. 1 hit. SM00211. TY. 1 hit. [Graphical view]
SUPFAM	SSF57184. Grow_fac_recept. 1 hit. SSF57610. Thyroglobulin_1. 1 hit.
PROSITE	PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	606017.

Entry information

Entry name

IBP5_RAT

Accession

Primary (citable) accession number: P24594

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	April 3, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program