Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P24592 (IBP6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 150. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Insulin-like growth factor-binding protein 6

Short name=IBP-6
Short name=IGF-binding protein 6
Short name=IGFBP-6
Gene names
Name:IGFBP6
Synonyms:IBP6
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length240 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Subcellular location

Secreted.

Post-translational modification

O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid. Ref.10

Sequence similarities

Contains 1 IGFBP N-terminal domain.

Contains 1 thyroglobulin type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Probable
Chain28 – 240213Insulin-like growth factor-binding protein 6
PRO_0000014389

Regions

Domain28 – 10780IGFBP N-terminal
Domain160 – 23475Thyroglobulin type-1

Amino acid modifications

Glycosylation1261O-linked (HexNAc...) Ref.10 Ref.12
Glycosylation1441O-linked (HexNAc...) By similarity
Glycosylation1451O-linked (HexNAc...) By similarity
Glycosylation1461O-linked (HexNAc...) By similarity
Glycosylation1521O-linked (HexNAc...) By similarity
Disulfide bond29 ↔ 32 Ref.11
Disulfide bond40 ↔ 44 Ref.11
Disulfide bond57 ↔ 63 Ref.11
Disulfide bond71 ↔ 84 Ref.11
Disulfide bond78 ↔ 104 Ref.11
Disulfide bond163 ↔ 190 Ref.11
Disulfide bond201 ↔ 212 Ref.11
Disulfide bond214 ↔ 234 Ref.11

Natural variations

Natural variant1281R → G. Ref.3
Corresponds to variant rs9658616 [ dbSNP | Ensembl ].
VAR_018932
Natural variant1341R → L.
Corresponds to variant rs34995393 [ dbSNP | Ensembl ].
VAR_049565
Natural variant2171R → Q. Ref.3
Corresponds to variant rs6413498 [ dbSNP | Ensembl ].
VAR_018933
Natural variant2361T → P.
Corresponds to variant rs1053134 [ dbSNP | Ensembl ].
VAR_011907

Experimental info

Sequence conflict21T → C in AAA88070. Ref.5
Sequence conflict28 – 292RC → AA AA sequence Ref.6
Sequence conflict28 – 292RC → LA AA sequence Ref.8
Sequence conflict321C → H AA sequence Ref.6
Sequence conflict55 – 573EGC → QGG AA sequence Ref.6

Secondary structure

............... 240
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P24592 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 285308231C025009

FASTA24025,322
        10         20         30         40         50         60 
MTPHRLLPPL LLLLALLLAA SPGGALARCP GCGQGVQAGC PGGCVEEEDG GSPAEGCAEA 

        70         80         90        100        110        120 
EGCLRREGQE CGVYTPNCAP GLQCHPPKDD EAPLRALLLG RGRCLPARAP AVAEENPKES 

       130        140        150        160        170        180 
KPQAGTARPQ DVNRRDQQRN PGTSTTPSQP NSAGVQDTEM GPCRRHLDSV LQQLQTEVYR 

       190        200        210        220        230        240 
GAQTLYVPNC DHRGFYRKRQ CRSSQGQRRG PCWCVDRMGK SLPGSPDGNG SSSCPTGSSG 

« Hide

References

« Hide 'large scale' references
[1]"Identification and molecular cloning of two new 30-kDa insulin-like growth factor binding proteins isolated from adult human serum."
Kiefer M.C., Masiarz F.R., Bauer D.M., Zapf J.
J. Biol. Chem. 266:9043-9049(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Osteosarcoma.
[2]"Characterization and chromosomal localization of the human insulin-like growth factor-binding protein 6 gene."
Ehrenborg E., Zazzi H., Lagercrantz S., Granqvist M., Hillerbrand U., Allander S.V., Larsson C., Luthman H.
Mamm. Genome 10:376-380(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]NIEHS SNPs program
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLY-128 AND GLN-217.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas.
[5]"Isolation and molecular cloning of insulin-like growth factor-binding protein-6."
Shimasaki S., Gao L., Shimonaka M., Ling N.
Mol. Endocrinol. 5:938-948(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-240.
Tissue: Placenta.
[6]"Isolation from adult human serum of four insulin-like growth factor (IGF) binding proteins and molecular cloning of one of them that is increased by IGF I administration and in extrapancreatic tumor hypoglycemia."
Zapf J., Kiefer M., Merryweather J., Musiarz F., Bauer D., Born W., Fischer J.A., Froesch E.R.
J. Biol. Chem. 265:14892-14898(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-57.
Tissue: Serum.
[7]"Isolation from human cerebrospinal fluid of a new insulin-like growth factor-binding protein with a selective affinity for IGF-II."
Roghani M., Hossenlopp P., Lepage P., Balland A., Binoux M.
FEBS Lett. 255:253-258(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY PROTEIN SEQUENCE OF 28-42.
Tissue: Cerebrospinal fluid.
[8]"Purification and properties of a novel insulin-like growth factor-II binding protein from transformed human fibroblasts."
Martin J.L., Willetts K.E., Baxter R.C.
J. Biol. Chem. 265:4124-4130(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-42.
Tissue: Fibroblast.
[9]"A novel human insulin-like growth factor binding protein secreted by osteoblast-like cells."
Andress D.L., Birnbaum R.S.
Biochem. Biophys. Res. Commun. 176:213-218(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-42.
Tissue: Osteosarcoma.
[10]"Identification of O-glycosylation sites and partial characterization of carbohydrate structure and disulfide linkages of human insulin-like growth factor binding protein 6."
Neumann G.M., Marinaro J.A., Bach L.A.
Biochemistry 37:6572-6585(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT THR-126; SER-144; THR-145; THR-146 AND SER-152.
[11]"The N-terminal disulfide linkages of human insulin-like growth factor-binding protein-6 (hIGFBP-6) and hIGFBP-1 are different as determined by mass spectrometry."
Neumann G.M., Bach L.A.
J. Biol. Chem. 274:14587-14594(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BONDS.
[12]"Enrichment of glycopeptides for glycan structure and attachment site identification."
Nilsson J., Rueetschi U., Halim A., Hesse C., Carlsohn E., Brinkmalm G., Larson G.
Nat. Methods 6:809-811(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT THR-126, STRUCTURE OF CARBOHYDRATES.
Tissue: Cerebrospinal fluid.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M62402 mRNA. Translation: AAB06187.1.
AJ006952 Genomic DNA. Translation: CAA07346.1.
AY443494 Genomic DNA. Translation: AAR05445.1.
BC003507 mRNA. Translation: AAH03507.1.
BC005007 mRNA. Translation: AAH05007.1.
BC010162 mRNA. Translation: AAH10162.1.
BC011708 mRNA. Translation: AAH11708.1.
M69054 mRNA. Translation: AAA88070.1.
CCDSCCDS8846.1.
PIRA39842.
S05699.
RefSeqNP_002169.1. NM_002178.2.
UniGeneHs.274313.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1RMJNMR-A161-240[»]
DisProtDP00211.
ProteinModelPortalP24592.
SMRP24592. Positions 25-105, 161-240.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109710. 2 interactions.
IntActP24592. 1 interaction.
MINTMINT-2859943.
STRING9606.ENSP00000301464.

Chemistry

BindingDBP24592.
ChEMBLCHEMBL2139.

Polymorphism databases

DMDM124068.

Proteomic databases

MaxQBP24592.
PaxDbP24592.
PeptideAtlasP24592.
PRIDEP24592.

Protocols and materials databases

DNASU3489.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000301464; ENSP00000301464; ENSG00000167779.
GeneID3489.
KEGGhsa:3489.
UCSCuc001sbu.1. human.

Organism-specific databases

CTD3489.
GeneCardsGC12P053491.
HGNCHGNC:5475. IGFBP6.
HPAHPA008005.
MIM146735. gene.
neXtProtNX_P24592.
PharmGKBPA29708.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG47794.
HOGENOMHOG000253012.
HOVERGENHBG002631.
InParanoidP24592.
OMARDQQRNP.
PhylomeDBP24592.
TreeFamTF331211.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.

Gene expression databases

ArrayExpressP24592.
BgeeP24592.
CleanExHS_IGFBP6.
GenevestigatorP24592.

Family and domain databases

Gene3D4.10.40.20. 1 hit.
4.10.800.10. 1 hit.
InterProIPR009030. Growth_fac_rcpt_N_dom.
IPR022326. IGFBP-6.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERPTHR11551. PTHR11551. 1 hit.
PfamPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSPR01976. IGFBPFAMILY.
PR01982. IGFBPFAMILY6.
SMARTSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEPS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP24592.
GeneWikiIGFBP6.
GenomeRNAi3489.
NextBio13722.
PROP24592.
SOURCESearch...

Entry information

Entry nameIBP6_HUMAN
AccessionPrimary (citable) accession number: P24592
Secondary accession number(s): Q14492
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: July 9, 2014
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM