Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P24588

- AKAP5_HUMAN

UniProt

P24588 - AKAP5_HUMAN

Protein

A-kinase anchor protein 5

Gene

AKAP5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 3 (15 Dec 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade.

    GO - Molecular functioni

    1. adenylate cyclase binding Source: UniProtKB
    2. protein binding Source: IntAct
    3. protein kinase A binding Source: ProtInc

    GO - Biological processi

    1. energy reserve metabolic process Source: Reactome
    2. positive regulation of cAMP biosynthetic process Source: UniProtKB
    3. positive regulation of protein kinase A signaling Source: UniProtKB
    4. protein targeting Source: InterPro
    5. regulation of insulin secretion Source: Reactome
    6. signal transduction Source: ProtInc
    7. small molecule metabolic process Source: Reactome
    8. synaptic transmission Source: Reactome

    Keywords - Ligandi

    Calmodulin-binding

    Enzyme and pathway databases

    ReactomeiREACT_18274. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
    REACT_18307. Trafficking of AMPA receptors.
    SignaLinkiP24588.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    A-kinase anchor protein 5
    Short name:
    AKAP-5
    Alternative name(s):
    A-kinase anchor protein 79 kDa
    Short name:
    AKAP 79
    H21
    cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
    Gene namesi
    Name:AKAP5
    Synonyms:AKAP79
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:375. AKAP5.

    Subcellular locationi

    Membrane 1 Publication; Lipid-anchor 1 Publication
    Note: Associates with lipid rafts.

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi392 – 3921L → P: Prevents or diminishes RII binding. 1 Publication
    Mutagenesisi396 – 3961A → P: Prevents or diminishes RII binding. 1 Publication
    Mutagenesisi400 – 4001V → P: Prevents or diminishes RII binding. 1 Publication
    Mutagenesisi405 – 4051Q → P: Prevents or diminishes RII binding. 1 Publication
    Mutagenesisi408 – 4081I → P: Prevents or diminishes RII binding. 1 Publication

    Organism-specific databases

    PharmGKBiPA24669.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 427427A-kinase anchor protein 5PRO_0000064529Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi36 – 361S-palmitoyl cysteine1 Publication
    Lipidationi129 – 1291S-palmitoyl cysteine1 Publication

    Post-translational modificationi

    Palmitoylation at Cys-36 and Cys-129 plays a key role in targeting to lipid rafts.1 Publication

    Keywords - PTMi

    Lipoprotein, Palmitate

    Proteomic databases

    MaxQBiP24588.
    PaxDbiP24588.
    PRIDEiP24588.

    PTM databases

    PhosphoSiteiP24588.

    Expressioni

    Tissue specificityi

    Predominantly in the cerebral cortex and the postsynaptic densities of the forebrain, and to a lesser extent in adrenal medulla, lung and anterior pituitary.

    Gene expression databases

    BgeeiP24588.
    CleanExiHS_AKAP5.
    GenevestigatoriP24588.

    Organism-specific databases

    HPAiCAB004308.

    Interactioni

    Subunit structurei

    Binding protein for dimer of the RII-beta regulatory subunit of cAMP-dependent protein kinase (PKA) and also for the protein kinase C (PKC) and the phosphatase calcineurin (PP2B). Each enzyme is inhibited when bound to the anchoring protein. Also binds the beta2-adrenergic receptor. Part of a complex containing AKAP5, ADCY5, ADCY6 AND PDE4C By similarity. Interacts with ADCY8, and enhances its phosphorylation at lipid rafts.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Kcnj2P355612EBI-703640,EBI-703793From a different organism.
    RXFP1Q9HBX92EBI-703640,EBI-8088969

    Protein-protein interaction databases

    BioGridi114874. 14 interactions.
    DIPiDIP-186N.
    IntActiP24588. 8 interactions.
    MINTiMINT-1733931.
    STRINGi9606.ENSP00000315615.

    Structurei

    Secondary structure

    1
    427
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi339 – 3446
    Helixi393 – 40816
    Turni409 – 4113

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2H9RNMR-C391-412[»]
    3LL8X-ray2.00E336-346[»]
    ProteinModelPortaliP24588.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP24588.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini73 – 10331AKAPAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 170170Essential to the intracellular anchoring functionBy similarityAdd
    BLAST
    Regioni392 – 40514PKA-RII subunit binding domainAdd
    BLAST

    Domaini

    RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
    The N-terminal region, which is highly basic, is required for interaction with calmodulin.

    Sequence similaritiesi

    Contains 1 AKAP domain.Curated

    Phylogenomic databases

    eggNOGiNOG25796.
    HOGENOMiHOG000168350.
    HOVERGENiHBG050479.
    InParanoidiP24588.
    KOiK16522.
    OMAiEMASDDN.
    OrthoDBiEOG73V6JZ.
    PhylomeDBiP24588.
    TreeFamiTF105404.

    Family and domain databases

    InterProiIPR001573. Pkinase-A_anch_WSK-motif.
    [Graphical view]
    PfamiPF03832. WSK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24588-1 [UniParc]FASTAAdd to Basket

    « Hide

    METTISEIHV ENKDEKRSAE GSPGAERQKE KASMLCFKRR KKAAKALKPK    50
    AGSEAADVAR KCPQEAGASD QPEPTRGAWA SLKRLVTRRK RSESSKQQKP 100
    LEGEMQPAIN AEDADLSKKK AKSRLKIPCI KFPRGPKRSN HSKIIEDSDC 150
    SIKVQEEAEI LDIQTQTPLN DQATKAKSTQ DLSEGISRKD GDEVCESNVS 200
    NSTTSGEKVI SVELGLDNGH SAIQTGTLIL EEIETIKEKQ DVQPQQASPL 250
    ETSETDHQQP VLSDVPPLPA IPDQQIVEEA SNSTLESAPN GKDYESTEIV 300
    AEETKPKDTE LSQESDFKEN GITEEKSKSE ESKRMEPIAI IITDTEISEF 350
    DVTKSKNVPK QFLISAENEQ VGVFANDNGF EDRTSEQYET LLIETASSLV 400
    KNAIQLSIEQ LVNEMASDDN KINNLLQ 427
    Length:427
    Mass (Da):47,088
    Last modified:December 15, 2009 - v3
    Checksum:iD2A86298EAF1BE32
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti188 – 1881R → Q in AAA58363. (PubMed:1512224)Curated
    Sequence conflicti407 – 4071S → Y no nucleotide entry (PubMed:1733921)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti100 – 1001P → L.
    Corresponds to variant rs2230491 [ dbSNP | Ensembl ].
    VAR_056732
    Natural varianti203 – 2031T → I.4 Publications
    Corresponds to variant rs1256149 [ dbSNP | Ensembl ].
    VAR_060735
    Natural varianti314 – 3141E → K.
    Corresponds to variant rs34433837 [ dbSNP | Ensembl ].
    VAR_056733

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90359 mRNA. Translation: AAA58363.1.
    AK315336 mRNA. Translation: BAG37736.1.
    AL122035 Genomic DNA. No translation available.
    CH471061 Genomic DNA. Translation: EAW80862.1.
    BC131516 mRNA. Translation: AAI31517.1.
    CCDSiCCDS9764.1.
    PIRiA43453.
    RefSeqiNP_004848.3. NM_004857.3.
    UniGeneiHs.656683.

    Genome annotation databases

    EnsembliENST00000320636; ENSP00000315615; ENSG00000179841.
    ENST00000394718; ENSP00000378207; ENSG00000179841.
    GeneIDi9495.
    KEGGihsa:9495.
    UCSCiuc001xhd.4. human.

    Polymorphism databases

    DMDMi281185503.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90359 mRNA. Translation: AAA58363.1 .
    AK315336 mRNA. Translation: BAG37736.1 .
    AL122035 Genomic DNA. No translation available.
    CH471061 Genomic DNA. Translation: EAW80862.1 .
    BC131516 mRNA. Translation: AAI31517.1 .
    CCDSi CCDS9764.1.
    PIRi A43453.
    RefSeqi NP_004848.3. NM_004857.3.
    UniGenei Hs.656683.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2H9R NMR - C 391-412 [» ]
    3LL8 X-ray 2.00 E 336-346 [» ]
    ProteinModelPortali P24588.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114874. 14 interactions.
    DIPi DIP-186N.
    IntActi P24588. 8 interactions.
    MINTi MINT-1733931.
    STRINGi 9606.ENSP00000315615.

    PTM databases

    PhosphoSitei P24588.

    Polymorphism databases

    DMDMi 281185503.

    Proteomic databases

    MaxQBi P24588.
    PaxDbi P24588.
    PRIDEi P24588.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000320636 ; ENSP00000315615 ; ENSG00000179841 .
    ENST00000394718 ; ENSP00000378207 ; ENSG00000179841 .
    GeneIDi 9495.
    KEGGi hsa:9495.
    UCSCi uc001xhd.4. human.

    Organism-specific databases

    CTDi 9495.
    GeneCardsi GC14P064932.
    H-InvDB HIX0037749.
    HGNCi HGNC:375. AKAP5.
    HPAi CAB004308.
    MIMi 604688. gene.
    neXtProti NX_P24588.
    PharmGKBi PA24669.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG25796.
    HOGENOMi HOG000168350.
    HOVERGENi HBG050479.
    InParanoidi P24588.
    KOi K16522.
    OMAi EMASDDN.
    OrthoDBi EOG73V6JZ.
    PhylomeDBi P24588.
    TreeFami TF105404.

    Enzyme and pathway databases

    Reactomei REACT_18274. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
    REACT_18307. Trafficking of AMPA receptors.
    SignaLinki P24588.

    Miscellaneous databases

    EvolutionaryTracei P24588.
    GeneWikii AKAP5.
    GenomeRNAii 9495.
    NextBioi 35572.
    PROi P24588.
    SOURCEi Search...

    Gene expression databases

    Bgeei P24588.
    CleanExi HS_AKAP5.
    Genevestigatori P24588.

    Family and domain databases

    InterProi IPR001573. Pkinase-A_anch_WSK-motif.
    [Graphical view ]
    Pfami PF03832. WSK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Localization of the cAMP-dependent protein kinase to the postsynaptic densities by A-kinase anchoring proteins. Characterization of AKAP 79."
      Carr D.W., Stofko-Hahn R.E., Fraser I.D.C., Cone R.D., Scott J.D.
      J. Biol. Chem. 267:16816-16823(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS, VARIANT ILE-203.
      Tissue: Thyroid.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-203.
      Tissue: Spleen.
    3. "The DNA sequence and analysis of human chromosome 14."
      Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
      , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
      Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ILE-203.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-203.
    6. "Cloning and expression of an intron-less gene for AKAP 75, an anchor protein for the regulatory subunit of cAMP-dependent protein kinase II beta."
      Hirsch A.H., Glantz S.B., Li Y., You Y., Rubin C.S.
      J. Biol. Chem. 267:2131-2134(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 332-427.
      Tissue: Kidney.
    7. "Palmitoylation targets AKAP79 protein to lipid rafts and promotes its regulation of calcium-sensitive adenylyl cyclase type 8."
      Delint-Ramirez I., Willoughby D., Hammond G.V., Ayling L.J., Cooper D.M.
      J. Biol. Chem. 286:32962-32975(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION AT CYS-36 AND CYS-129, INTERACTION WITH ADCY8, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiAKAP5_HUMAN
    AccessioniPrimary (citable) accession number: P24588
    Secondary accession number(s): A2RRB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: December 15, 2009
    Last modified: October 1, 2014
    This is version 132 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3