Reviewed,
UniProtKB/Swiss-Prot P24588 (AKAP5_HUMAN)
Last modified
May 26, 2009.
Version 79.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: A-kinase anchor protein 5 Alternative name(s): A-kinase anchor protein 79 kDa Short name=AKAP 79 cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein H21 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 427 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade. |
| Subunit structure | Binding protein for dimer of the RII-beta regulatory subunit of cAMP-dependent protein kinase (PKA) and also for the protein kinase C (PKC) and the phosphatase calcineurin (PP2B). Each enzyme is inhibited when bound to the anchoring protein. Also binds the beta2-adrenergic receptor. |
| Subcellular location | Membrane. Note: Associated with particulate fractions. |
| Tissue specificity | Predominantly in the cerebral cortex and the postsynaptic densities of the forebrain, and to a lesser extent in adrenal medulla, lung and anterior pituitary. |
| Domain | RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer. |
| Miscellaneous | The N-terminal region, which is highly basic, is required for interaction with calmodulin. |
| Sequence similarities | Contains 1 AKAP domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Membrane |
| Coding sequence diversity | Polymorphism |
| Ligand | Calmodulin-binding |
| Technical term | 3D-structure |
| Gene Ontology (GO) | |
| Biological process | protein targeting Inferred from electronic annotation. Source: InterPro signal transduction Ref.1Non-traceable author statement. Source: ProtInc synaptic transmission Ref.1Traceable author statement. Source: ProtInc |
| Cellular component | plasma membrane Ref.1 Non-traceable author statement. Source: ProtInc |
| Molecular function | calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase A binding Ref.1Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| KCNJ2 | P63252 | 1 | EBI-703640,EBI-703457 | |
| Kcnj2 | P35561 | 2 | EBI-703640,EBI-703793 | From a different organism. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||
Molecule processing | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 427 | 427 | A-kinase anchor protein 5 | PRO_0000064529 | ||||||||
Regions | ||||||||||||
| Domain | 73 – 103 | 31 | AKAP | |||||||||
| Region | 1 – 170 | 170 | Essential to the intracellular anchoring function By similarity | |||||||||
| Region | 392 – 405 | 14 | PKA-RII subunit binding domain | |||||||||
Natural variations | ||||||||||||
| Natural variant | 100 | 1 | P → L: dbSNP rs2230491. | VAR_056732 | ||||||||
| Natural variant | 314 | 1 | E → K: dbSNP rs34433837. | VAR_056733 | ||||||||
Experimental info | ||||||||||||
| Mutagenesis | 392 | 1 | L → P: Prevents or diminishes RII binding. | |||||||||
| Mutagenesis | 396 | 1 | A → P: Prevents or diminishes RII binding. | |||||||||
| Mutagenesis | 400 | 1 | V → P: Prevents or diminishes RII binding. | |||||||||
| Mutagenesis | 405 | 1 | Q → P: Prevents or diminishes RII binding. | |||||||||
| Mutagenesis | 408 | 1 | I → P: Prevents or diminishes RII binding. | |||||||||
| Sequence conflict | 407 | 1 | S → Y Ref.2 | |||||||||
Secondary structure | ||||||||||||
Helix Strand Turn | ||||||||||||
| Helix | 393 – 408 | 16 | ||||||||||
| Turn | 409 – 411 | 3 | ||||||||||
Sequences
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References
| [1] | "Localization of the cAMP-dependent protein kinase to the postsynaptic densities by A-kinase anchoring proteins. Characterization of AKAP 79." Carr D.W., Stofko-Hahn R.E., Fraser I.D.C., Cone R.D., Scott J.D. J. Biol. Chem. 267:16816-16823(1992) [PubMed: 1512224] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS. Tissue: Thyroid. |
| [2] | "Cloning and expression of an intron-less gene for AKAP 75, an anchor protein for the regulatory subunit of cAMP-dependent protein kinase II beta." Hirsch A.H., Glantz S.B., Li Y., You Y., Rubin C.S. J. Biol. Chem. 267:2131-2134(1992) [PubMed: 1733921] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 332-427. Tissue: Kidney. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M90359 mRNA. Translation: AAA58363.1. | |||||||||||||
| IPI | IPI00307794. | ||||||||||||
| PIR | A43453. | ||||||||||||
| UniGene | Hs.656683 | ||||||||||||
3D structure databases | |||||||||||||
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| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| DIP | DIP:186N. | ||||||||||||
| IntAct | P24588. 2 interactions. | ||||||||||||
Proteomic databases | |||||||||||||
| PRIDE | P24588. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSG00000179841. Homo sapiens. [Contig view] | ||||||||||||
| KEGG | hsa:9495. | ||||||||||||
Organism-specific databases | |||||||||||||
| GeneCards | GC14P064003. | ||||||||||||
| H-InvDB | HIX0037749. | ||||||||||||
| HGNC | HGNC:375. AKAP5. | ||||||||||||
| HPA | CAB004308. | ||||||||||||
| MIM | 604688. gene. | ||||||||||||
| PharmGKB | PA24669. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | P24588. | ||||||||||||
| HOVERGEN | P24588. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| Pathway_Interaction_DB | tcrcalciumpathway. Calcium signaling in the CD4+ TCR pathway. nfat_3pathway. Role of Calcineurin-dependent NFAT signaling in lymphocytes. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | P24588. | ||||||||||||
| Bgee | P24588. | ||||||||||||
| CleanEx | HS_AKAP5. | ||||||||||||
| GermOnline | ENSG00000179841. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR001573. Pkinase-A_anch_WSK-motif. IPR018459. RII_binding_1. [Graphical view] | ||||||||||||
| Pfam | PF10522. RII_binding_1. 1 hit. PF03832. WSK. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | AKAP5_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P24588 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 14 Human chromosome 14: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


