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Protein

A-kinase anchor protein 5

Gene

Akap5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May anchor the PKA kinase to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors.

GO - Molecular functioni

  • actin binding Source: RGD
  • adenylate cyclase binding Source: RGD
  • beta-2 adrenergic receptor binding Source: ARUK-UCL
  • cadherin binding Source: RGD
  • calmodulin binding Source: UniProtKB-KW
  • GABA receptor binding Source: RGD
  • glutamate receptor binding Source: SynGO-UCL
  • G-protein coupled receptor binding Source: RGD
  • kinase binding Source: RGD
  • protein complex binding Source: RGD
  • protein complex scaffold activity Source: SynGO-UCL
  • protein domain specific binding Source: RGD
  • protein kinase A regulatory subunit binding Source: RGD
  • protein kinase binding Source: RGD
  • protein phosphatase 2B binding Source: RGD
  • scaffold protein binding Source: SynGO-UCL
  • SH3 domain binding Source: SynGO-UCL

GO - Biological processi

  • cellular protein complex disassembly Source: RGD
  • cellular response to organonitrogen compound Source: RGD
  • hippocampus development Source: RGD
  • maternal process involved in female pregnancy Source: RGD
  • negative regulation of adenylate cyclase activity Source: RGD
  • negative regulation of ion transport Source: RGD
  • negative regulation of potassium ion transport Source: RGD
  • positive regulation of adenylate cyclase activity Source: RGD
  • positive regulation of calcium ion transport into cytosol Source: RGD
  • positive regulation of dendrite morphogenesis Source: RGD
  • positive regulation of NFAT protein import into nucleus Source: RGD
  • positive regulation of protein phosphorylation Source: RGD
  • protein targeting Source: InterPro
  • regulation of insulin secretion Source: Reactome
  • response to electrical stimulus Source: RGD
  • response to muscle activity Source: RGD
  • signal transduction Source: InterPro
  • spinal cord development Source: RGD

Keywordsi

Molecular functionCalmodulin-binding

Enzyme and pathway databases

ReactomeiR-RNO-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 5
Short name:
AKAP-5
Alternative name(s):
A-kinase anchor protein 150 kDa
Short name:
AKAP 150
Short name:
P150
cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
Gene namesi
Name:Akap5
Synonyms:Akap150
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620829. Akap5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000645161 – 714A-kinase anchor protein 5Add BLAST714

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1
Modified residuei22PhosphoserineCombined sources1
Lipidationi36S-palmitoyl cysteineBy similarity1
Lipidationi123S-palmitoyl cysteineBy similarity1

Post-translational modificationi

Palmitoylation at Cys-36 and Cys-123 plays a key role in targeting to lipid rafts.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP24587.
PRIDEiP24587.

PTM databases

iPTMnetiP24587.
PhosphoSitePlusiP24587.
SwissPalmiP24587.

Interactioni

Subunit structurei

Interacts with ADCY8, and enhances its phosphorylation at lipid rafts (By similarity). Binds dimer of the RII-beta regulatory subunit of cAMP-dependent protein kinase.By similarity

GO - Molecular functioni

  • actin binding Source: RGD
  • adenylate cyclase binding Source: RGD
  • beta-2 adrenergic receptor binding Source: ARUK-UCL
  • cadherin binding Source: RGD
  • calmodulin binding Source: UniProtKB-KW
  • GABA receptor binding Source: RGD
  • glutamate receptor binding Source: SynGO-UCL
  • G-protein coupled receptor binding Source: RGD
  • kinase binding Source: RGD
  • protein complex binding Source: RGD
  • protein complex scaffold activity Source: SynGO-UCL
  • protein domain specific binding Source: RGD
  • protein kinase A regulatory subunit binding Source: RGD
  • protein kinase binding Source: RGD
  • protein phosphatase 2B binding Source: RGD
  • scaffold protein binding Source: SynGO-UCL
  • SH3 domain binding Source: SynGO-UCL

Protein-protein interaction databases

CORUMiP24587.
IntActiP24587. 1 interactor.
STRINGi10116.ENSRNOP00000008416.

Structurei

3D structure databases

SMRiP24587.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 101AKAPAdd BLAST30
Repeati305 – 3121; approximate8
Repeati322 – 3292; approximate8
Repeati330 – 3373; approximate8
Repeati350 – 3574; approximate8
Repeati358 – 3655; approximate8
Repeati366 – 3736; approximate8
Repeati398 – 4057; approximate8
Repeati414 – 4218; approximate8
Repeati430 – 43798
Repeati438 – 445108
Repeati446 – 453118
Repeati454 – 461128
Repeati462 – 469138
Repeati470 – 47714; approximate8
Repeati486 – 49315; approximate8
Repeati494 – 501168
Repeati502 – 509178
Repeati510 – 517188
Repeati518 – 525198
Repeati526 – 53320; approximate8
Repeati534 – 541218
Repeati542 – 54922; approximate8
Repeati550 – 55723; approximate8
Repeati558 – 56524; approximate8
Repeati566 – 573258
Repeati574 – 58126; approximate8
Repeati582 – 58927; approximate8
Repeati590 – 59728; approximate8

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 164Essential to the intracellular anchoring functionBy similarityAdd BLAST164
Regioni305 – 59728 X 8 AA repeats of V-G-Q-A-E-E-A-TAdd BLAST293
Regioni675 – 696RII-beta subunit binding domainBy similarityAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi326 – 595Glu-richAdd BLAST270

Domaini

The N-terminal region, which is highly basic, is required for interaction with calmodulin.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IIWH. Eukaryota.
ENOG4111833. LUCA.
InParanoidiP24587.
PhylomeDBiP24587.

Family and domain databases

InterProiView protein in InterPro
IPR001573. Pkinase-A_anch_WSK-motif.
PfamiView protein in Pfam
PF03832. WSK. 1 hit.

Sequencei

Sequence statusi: Complete.

P24587-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METSVSEIQI ETKDEKRPEA ASPQKERQER KTATLCFKRR KKVNKKKAKA
60 70 80 90 100
GSKTAEETEK HAPEAGGSGQ RQPAGAWASI KRLVTHRKPS ESAEKQKPSE
110 120 130 140 150
AEMQPEDGAL PKKKTKSKLK IPCIRFSRGA KRSRPSKLTE DSGYVRVQGE
160 170 180 190 200
ADDLEIKAQI QPDEQATQAK STQGLQEDVI VRDGKEIQES HISNNVISGE
210 220 230 240 250
HVIGIELELE KESSALRMRT PGSEKEAKVI LVKQGVQVQE ASVLENSAAD
260 270 280 290 300
SPQPVTSTAP LSPATTHQLG LEEPSDSIRE SAPSGKDDGR RKTAAEEKKS
310 320 330 340 350
GETALGQAEE ASSVSQADKS VLSQAEEATV GHTEEATVIQ AQSQAKEGKL
360 370 380 390 400
SQAEEATVAQ AKETVLSQAE EVKLSQIEEP AISQAKKATV GQAKEAYVSQ
410 420 430 440 450
AEEAIVGHTE KATMGQAEEA TVGHIEKTTV GQAEEATVGQ AEEATVGQAE
460 470 480 490 500
EATVGQAEEA TVGQAEEATV GQAGEATVSH IEKTTVGQAE EAIVGQAEEA
510 520 530 540 550
TVGQAEEATV GQAEEATVGQ AEEATVDQAE EATVGQAEEA TVGQAGEAAV
560 570 580 590 600
GQAEEAIVAQ AEEATVGQAG EATVGQAEKA TVGQAEEPIV GQAEETVLRH
610 620 630 640 650
ASDLKVNGVD AEKPRSEESK RMEPIAIIIT DTEISEFDVK KSKNVPKQFL
660 670 680 690 700
ISMENEQVGV FANDSDFEGR TSEQYETLLI ETASSLVKNA IELSVEQLVN
710
EMVSEDNQIN TLFQ
Length:714
Mass (Da):75,962
Last modified:December 6, 2005 - v2
Checksum:i933ED7E0CD236AEA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti251S → G in AAA50420 (PubMed:2538452).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67136 mRNA. Translation: AAB07887.1.
J04597 mRNA. Translation: AAA50420.1.
PIRiA32461.
UniGeneiRn.122003.
Rn.215609.

Genome annotation databases

UCSCiRGD:620829. rat.

Similar proteinsi

Entry informationi

Entry nameiAKAP5_RAT
AccessioniPrimary (citable) accession number: P24587
Secondary accession number(s): P70593
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: December 6, 2005
Last modified: October 25, 2017
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome